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Gene information
General information
TC005611 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3574580..3575701
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005611
    iBeetle screen iB_03988
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005611 RA
      MSSILEEKL REAACIGDI EAVLALLSQ 
      NLDINAKNS VNGWTALHW AAKRGNSQV 
      ADILLSHGA NPKIQNLKG EDPSQAPLD 
      DAKFTPNYL KNAPVNGQV DIGKLRGKP 
      DFSAMPTTA LPACLNDDL VLNVRIAGS 
      SDPDFIEVE IPRWKLTYL NLFKICCEE 
      LEVLESQVE RIRKLPSTR LRKDSDVKR 
      LDNFQSLEL VLKMPLGSD KPNNCYQSI 
      STCKNQTIL Y
    • >TC005611 RB
      MSSILEEKL REAACIGDI EAVLALLSQ NLDINAKNS 
      VNGWTALHW AAKRGNSQV ADILLSHGA NPKIQNLKG 
      ETPGNLCTN PETLALLGE DPSQAPLDD AKFTPNYLK 
      NAPVNGQVD IGKLRGKPD FSAMPTTAL PACLNDDLV 
      LNVRIAGSS DPDFIEVEI PRWKLTYLN LFKICCEEL 
      EVLESQVER IRKLPSTRL RKDSDVKRL DNFQSLELV 
      LKMPLGSDK PNNCYQSIS TCKNQTILY
    CDS sequence
    • >TC005611 RA
      atgagctca attcttgaa gaaaaactg cgtgaagcc 
      gcctgtatt ggggacatc gaggcagtc ctagccctc 
      ctatcgcaa aatctcgac ataaacgca aaaaacagc 
      gtcaacgga tggacggcc ctgcactgg gcggctaaa 
      cgcggcaat tcgcaagtg gccgatatt ctcctaagc 
      cacggcgcg aatcccaag attcagaac ttgaaaggg 
      gaagacccg agtcaagcc cctctcgac gacgcgaaa 
      ttcactccc aattatctc aaaaacgcg ccggtcaac 
      ggccaagtt gacatcggg aaactgagg ggcaagccg 
      gacttctcg gccatgccc accacggcg ctcccggcg 
      tgtctcaat gatgatctc gtgctaaat gtgcgcatt 
      gcgggttca agcgacccc gattttatc gaagtggag 
      attccgcgc tggaagttg acttatctc aacttgttc 
      aagatctgt tgtgaggag ttggaggtg ttggagtct 
      caagttgag aggattcgg aagttgccg agtacgaga 
      ctgaggaaa gatagtgat gttaagaga ttggataat 
      ttccagagt ctcgagttg gtgctcaag atgcccctg 
      ggcagtgat aaacccaac aattgctat cagagtata 
      tcaacttgt aagaatcaa acaatttta tactaa
    • >TC005611 RB
      atgagctca attcttgaa gaaaaactg cgtgaagcc 
      gcctgtatt ggggacatc gaggcagtc ctagccctc 
      ctatcgcaa aatctcgac ataaacgca aaaaacagc 
      gtcaacgga tggacggcc ctgcactgg gcggctaaa 
      cgcggcaat tcgcaagtg gccgatatt ctcctaagc 
      cacggcgcg aatcccaag attcagaac ttgaaaggt 
      gaaacgcct ggtaatctt tgtactaat cccgaaacg 
      cttgctttg ttaggggaa gacccgagt caagcccct 
      ctcgacgac gcgaaattc actcccaat tatctcaaa 
      aacgcgccg gtcaacggc caagttgac atcgggaaa 
      ctgaggggc aagccggac ttctcggcc atgcccacc 
      acggcgctc ccggcgtgt ctcaatgat gatctcgtg 
      ctaaatgtg cgcattgcg ggttcaagc gaccccgat 
      tttatcgaa gtggagatt ccgcgctgg aagttgact 
      tatctcaac ttgttcaag atctgttgt gaggagttg 
      gaggtgttg gagtctcaa gttgagagg attcggaag 
      ttgccgagt acgagactg aggaaagat agtgatgtt 
      aagagattg gataatttc cagagtctc gagttggtg 
      ctcaagatg cccctgggc agtgataaa cccaacaat 
      tgctatcag agtatatca acttgtaag aatcaaaca 
      attttatac taa
    mRNA sequence
    • >TC005611 RA
      acatgcgta ttgagtttc aaaaatgta aaattaatc 
      gagactgtg ccacttcgt tacgtttct gtaaacccc 
      acgcgcttt ctagcccca atcacgcca catgagacg 
      gccctcatt cccccaaca ctgttgcgg tgtctcaaa 
      taacctccc aaaaaatga accatgagc tcaattctt 
      gaagaaaaa ctgcgtgaa gccgcctgt attggggac 
      atcgaggca gtcctagcc ctcctatcg caaaatctc 
      gacataaac gcaaaaaac agcgtcaac ggatggacg 
      gccctgcac tgggcggct aaacgcggc aattcgcaa 
      gtggccgat attctccta agccacggc gcgaatccc 
      aagattcag aacttgaaa ggggaagac ccgagtcaa 
      gcccctctc gacgacgcg aaattcact cccaattat 
      ctcaaaaac gcgccggtc aacggccaa gttgacatc 
      gggaaactg aggggcaag ccggacttc tcggccatg 
      cccaccacg gcgctcccg gcgtgtctc aatgatgat 
      ctcgtgcta aatgtgcgc attgcgggt tcaagcgac 
      cccgatttt atcgaagtg gagattccg cgctggaag 
      ttgacttat ctcaacttg ttcaagatc tgttgtgag 
      gagttggag gtgttggag tctcaagtt gagaggatt 
      cggaagttg ccgagtacg agactgagg aaagatagt 
      gatgttaag agattggat aatttccag agtctcgag 
      ttggtgctc aagatgccc ctgggcagt gataaaccc 
      aacaattgc tatcagagt atatcaact tgtaagaat 
      caaacaatt ttatactaa gggaacgca ttctaaagt 
      tcggaactc cacgatttc tgtctattt attatagag 
      ctgctagtt tgaataaag ttattgctt cgga
    • >TC005611 RB
      acatgcgta ttgagtttc aaaaatgta aaattaatc 
      gagactgtg ccacttcgt tacgtttct gtaaacccc 
      acgcgcttt ctagcccca atcacgcca catgagacg 
      gccctcatt cccccaaca ctgttgcgg tgtctcaaa 
      taacctccc aaaaaatga accatgagc tcaattctt 
      gaagaaaaa ctgcgtgaa gccgcctgt attggggac 
      atcgaggca gtcctagcc ctcctatcg caaaatctc 
      gacataaac gcaaaaaac agcgtcaac ggatggacg 
      gccctgcac tgggcggct aaacgcggc aattcgcaa 
      gtggccgat attctccta agccacggc gcgaatccc 
      aagattcag aacttgaaa ggtgaaacg cctggtaat 
      ctttgtact aatcccgaa acgcttgct ttgttaggg 
      gaagacccg agtcaagcc cctctcgac gacgcgaaa 
      ttcactccc aattatctc aaaaacgcg ccggtcaac 
      ggccaagtt gacatcggg aaactgagg ggcaagccg 
      gacttctcg gccatgccc accacggcg ctcccggcg 
      tgtctcaat gatgatctc gtgctaaat gtgcgcatt 
      gcgggttca agcgacccc gattttatc gaagtggag 
      attccgcgc tggaagttg acttatctc aacttgttc 
      aagatctgt tgtgaggag ttggaggtg ttggagtct 
      caagttgag aggattcgg aagttgccg agtacgaga 
      ctgaggaaa gatagtgat gttaagaga ttggataat 
      ttccagagt ctcgagttg gtgctcaag atgcccctg 
      ggcagtgat aaacccaac aattgctat cagagtata 
      tcaacttgt aagaatcaa acaatttta tactaaggg 
      aacgcattc taaagttcg gaactccac gatttctgt 
      ctatttatt atagagctg ctagtttga ataaagtta 
      ttgcttcgg a