Status
Please wait ...
Gene information
General information
TC005609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3579097..3580588
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005609
    iBeetle screen iB_03987
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005609 RA
      MATFQIHED VYSTLAENK KPKNFEPVK SLKSNIPRV 
      ALQPIHTFQ QNAKRPKPV EKDEAVKMP PVSTTVVSP 
      AQVEWPVKT IRLKPVFKK NNLIAKANS FEKEYEDEI 
      LEYLFGFEK RAQIIKPYY MTKQLHLNW EMRSVLVDW 
      LVSVAHEYK MSNNTVHLT VNYLDRFLS NISVIRSKF 
      QLVGATAMM IAGKVEDVY PPDSKEWSY LTRDAFSVN 
      EIHKMEQFM LRVLNFEMN PPTILDFIK RLCSKHKVD 
      KKTTYLAMY LSELVLLEG DEYLQHFPS KLAAASLAL 
      ARHTLAKPE PWNRKLEET AGYTLKQLS PVVQKQHKT 
      FKSSPFKEQ QAIQKKYAS VKYQRVALL KPRILQLDD 
      EE
    CDS sequence
    • >TC005609 RA
      atggccacg tttcaaata cacgaagat gtatactcc 
      actttggcc gaaaataag aagccgaag aatttcgag 
      cctgtgaaa agtctcaag tcgaatatc ccacgagtc 
      gcccttcag cccatccac accttccag cagaacgca 
      aagcgaccc aagccggtg gaaaaagac gaagctgtg 
      aaaatgcca ccggtgagt acaaccgtg gtgtcccca 
      gcccaagtc gagtggccc gttaaaacg atcagactt 
      aaaccagtg ttcaaaaaa aataatctg atcgcgaaa 
      gcgaattcg tttgagaag gagtacgag gacgagatt 
      ttggagtac ttgtttggt tttgagaaa agggcgcaa 
      atcataaaa ccgtactat atgacgaag cagttgcac 
      ctgaactgg gagatgagg agtgtgctt gtagattgg 
      ctcgtgagc gtggctcac gagtacaag atgagtaac 
      aacacggtg catttgacg gtaaactat ctcgaccgg 
      tttttgagc aacatctcg gtgataaga agcaaattc 
      caattggtt ggagccacg gcgatgatg atcgccggc 
      aaagtggag gatgtgtac ccaccggac tccaaggag 
      tggtcctac ttgaccaga gacgcattc agtgttaat 
      gagatccat aaaatggag cagttcatg ctcagagtc 
      ctcaatttt gagatgaac ccgcccacg attttggat 
      ttcatcaaa cggctctgc tccaaacac aaggtggac 
      aagaaaacc acttaccta gccatgtat ttgagcgag 
      ttggtgctt ctcgaaggc gacgaatac ttgcagcac 
      tttccgtcg aaattagca gcggcgtct ttggccctc 
      gcgcgccac actttagcc aagccggag ccctggaac 
      aggaaattg gaggaaacg gccggttac accttgaaa 
      cagttgagt ccagtggtc cagaagcag cacaaaacg 
      ttcaagagt tcaccgttt aaggaacaa caagccatt 
      cagaagaaa tatgccagt gtaaagtac caaagggtg 
      gcgcttcta aaaccgcga attctgcag cttgatgat 
      gaagagtga
    mRNA sequence
    • >TC005609 RA
      caaatttat tggtattaa ttactacat aacggccgc 
      aagacgcgt aatagcgcg ccgcttaag ttttaattt 
      tttggccaa ggaggggtt tccttgacg aagcgtttt 
      ttaaattga aagaggcgc gcaaaaaat caacataca 
      aaaaagttt gacaacgtt tatctcttg taaaatttt 
      acaagacgt tcgccgccc agttttacc tatttctac 
      cgtactttt gccccaaaa atggccacg tttcaaata 
      cacgaagat gtatactcc actttggcc gaaaataag 
      aagccgaag aatttcgag cctgtgaaa agtctcaag 
      tcgaatatc ccacgagtc gcccttcag cccatccac 
      accttccag cagaacgca aagcgaccc aagccggtg 
      gaaaaagac gaagctgtg aaaatgcca ccggtgagt 
      acaaccgtg gtgtcccca gcccaagtc gagtggccc 
      gttaaaacg atcagactt aaaccagtg ttcaaaaaa 
      aataatctg atcgcgaaa gcgaattcg tttgagaag 
      gagtacgag gacgagatt ttggagtac ttgtttggt 
      tttgagaaa agggcgcaa atcataaaa ccgtactat 
      atgacgaag cagttgcac ctgaactgg gagatgagg 
      agtgtgctt gtagattgg ctcgtgagc gtggctcac 
      gagtacaag atgagtaac aacacggtg catttgacg 
      gtaaactat ctcgaccgg tttttgagc aacatctcg 
      gtgataaga agcaaattc caattggtt ggagccacg 
      gcgatgatg atcgccggc aaagtggag gatgtgtac 
      ccaccggac tccaaggag tggtcctac ttgaccaga 
      gacgcattc agtgttaat gagatccat aaaatggag 
      cagttcatg ctcagagtc ctcaatttt gagatgaac 
      ccgcccacg attttggat ttcatcaaa cggctctgc 
      tccaaacac aaggtggac aagaaaacc acttaccta 
      gccatgtat ttgagcgag ttggtgctt ctcgaaggc 
      gacgaatac ttgcagcac tttccgtcg aaattagca 
      gcggcgtct ttggccctc gcgcgccac actttagcc 
      aagccggag ccctggaac aggaaattg gaggaaacg 
      gccggttac accttgaaa cagttgagt ccagtggtc 
      cagaagcag cacaaaacg ttcaagagt tcaccgttt 
      aaggaacaa caagccatt cagaagaaa tatgccagt 
      gtaaagtac caaagggtg gcgcttcta aaaccgcga 
      attctgcag cttgatgat gaagagtga ttatagaaa 
      taggtactt gtgactaca attgttcca attgttata 
      aattgtatg tggaattaa taaagtatt gtcaagtgt