Status
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Gene information
General information
TC005588 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10981637..10989386
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005588
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03984

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005588 RA
      MRRPMKRNK ITEGIYGST LLYLKFLII WVLVILADF 
      ILEFRFEFL WPFYLLLRS VYDSFKYQG LAFSIFFVC 
      IALTSDMLC FFFIPVHWL FFAASTYVW VQYVWHTDK 
      GICAPTIIL WVLFVYLEA AVRLRDVKH MPGHLDLCR 
      PFAAHCIGY PVVTLGFGF KSYIGYRMR QRKQRDVAK 
      ENEFYMQLM QQALPMDAL PPPPPAVQP PPEPVSTAI 
      VPAPVQNAK VHNNKLHEK NGHIPNGTI ANGVHATKS 
      HHRKSVDKC KEHDEHNRH SDKHIDRQR QVNHSHSNG 
      TAISAGVEE ITSTTATAT VQEHEPRPT RRREKKEQD 
      KEREAAEYL QKLEADAKR LRADLQSSR ASEQELRLQ 
      VAALTTSEK VIKGELTAM QREIEDVQQ RYQSVASSR 
      ASDKQTISN LERRVAEER RARCNLESQ LNQERKSRK 
      QEEARAAQV VAQSSRGEC TEACRARRR ELEAELSEC 
      RTTKHWAEE RYSALEREN AALVDQLRD MDILMTALT 
      AMQEKNAHL ENSLSAETR IKLDLFSAL GEAKRQLEI 
      RESSNRTQE REIEELKSK IAQVLAVMP NDSFGGPTV 
      ATSNMSRVR LAESPPGST LDPNATAYT PKSSLVAST 
      EA
    CDS sequence
    • >TC005588 RA
      atgagaaga cccatgaaa aggaacaaa atcaccgaa 
      gggatttac ggaagtact ctcctgtac ctgaagttc 
      ctgattata tgggttttg gtaattctg gccgatttc 
      atcctggag ttcagattt gagttccta tggccgttt 
      tatttgctg ctgcgttcg gtctatgac tccttcaaa 
      taccaaggc ctggccttt tcaattttt ttcgtgtgc 
      attgcgctc acttcagac atgctgtgc ttcttcttt 
      atcccggtt cattggctc ttcttcgcc gccagtacc 
      tatgtctgg gtgcagtac gtgtggcac acagacaag 
      ggcatctgt gcacccacc attattctg tgggtgctc 
      tttgtctat ttggaggcg gcagtgcgg ctgcgagac 
      gtcaaacac atgccaggc cacctggac ctgtgccgc 
      cccttcgcc gcccactgc atcggctac cccgtcgtc 
      accctgggg ttcgggttc aagtcctac atagggtat 
      aggatgcgg cagcgcaag cagcgcgac gtagcgaaa 
      gagaacgag ttttacatg cagctgatg cagcaggcg 
      ttgcctatg gacgcccta ccgccgccg ccgcctgcc 
      gtgcagccg cccccggag ccagtctcc acagccatc 
      gtacctgcc cccgtacaa aacgccaaa gtacataat 
      aataagttg cacgagaaa aacggccac atccccaat 
      gggacgata gccaacggt gtgcacgcg accaaatca 
      catcataga aagtctgtc gataaatgt aaagaacac 
      gacgaacac aacaggcat tcggataag cacatagat 
      agacaaaga caagttaat cattcgcac agtaatgga 
      acggcgatt agtgccggc gttgaagaa atcacgtcg 
      acgacggcg acggccacg gttcaagaa catgaaccg 
      agaccgacg aggcgaaga gaaaagaag gaacaggat 
      aaggagaga gaagcggcc gagtatctg caaaagctc 
      gaggccgac gctaaaagg ctgcgggcg gacctacaa 
      agcagcaga gccagcgag caagaactc aggctgcag 
      gttgccgcg ctcaccacg agcgagaag gtaatcaag 
      ggcgagctg acagcgatg caacgcgaa atcgaagac 
      gtgcaacag cgttatcaa agtgttgca agttcccgc 
      gcttccgat aaacagacg atcagcaac ttggaacgt 
      cgagtggct gaagaaagg cgagcaaga tgcaatcta 
      gaatcgcaa ctaaatcaa gaacgtaaa agtagaaaa 
      caagaggag gcccgagcg gctcaagtg gtggcacag 
      tcatcgcgc ggcgagtgc acggaagcg tgccgagcg 
      cgtcgtaga gaattagaa gccgaattg agcgagtgt 
      cggacaacg aagcattgg gccgaagaa cggtactcc 
      gcgctggaa cgcgaaaat gccgcccta gtcgatcag 
      ctccgtgat atggacata ctcatgacc gccctcacg 
      gcaatgcag gagaaaaac gcccattta gaaaattcg 
      ttatcggcc gagacgcgc atcaagctc gacctcttc 
      agcgccctg ggcgaggcg aagcgacaa ctcgaaatc 
      agagagagc tccaacagg acgcaagaa cgcgagatc 
      gaagagctg aagagcaaa atcgcgcaa gtgttggct 
      gtgatgccg aacgacagt ttcggcggt ccgacggtg 
      gcgacgagc aacatgtcg cgagtccgg ctggccgag 
      agtccgccc ggttcgacc ctggacccg aacgccacg 
      gcctacacc cctaagagt tcccttgtc gcttccacc 
      gaagcctag
    mRNA sequence
    • >TC005588 RA
      agcgcgcaa gttgacaac aaaagacat tttaccgca 
      aaagcgaag tttttgtga caaaaattc catgagaca 
      gccgagttt tttggcatt ttaccgctg ggagtgagt 
      cgactaata gttcggagc attggttcg gtctgaatt 
      atgaagaga cgaaatgct gagatcggt aagatgaga 
      agacccatg aaaaggaac aaaatcacc gaagggatt 
      tacggaagt actctcctg tacctgaag ttcctgatt 
      atatgggtt ttggtaatt ctggccgat ttcatcctg 
      gagttcaga tttgagttc ctatggccg ttttatttg 
      ctgctgcgt tcggtctat gactccttc aaataccaa 
      ggcctggcc ttttcaatt tttttcgtg tgcattgcg 
      ctcacttca gacatgctg tgcttcttc tttatcccg 
      gttcattgg ctcttcttc gccgccagt acctatgtc 
      tgggtgcag tacgtgtgg cacacagac aagggcatc 
      tgtgcaccc accattatt ctgtgggtg ctctttgtc 
      tatttggag gcggcagtg cggctgcga gacgtcaaa 
      cacatgcca ggccacctg gacctgtgc cgccccttc 
      gccgcccac tgcatcggc taccccgtc gtcaccctg 
      gggttcggg ttcaagtcc tacataggg tataggatg 
      cggcagcgc aagcagcgc gacgtagcg aaagagaac 
      gagttttac atgcagctg atgcagcag gcgttgcct 
      atggacgcc ctaccgccg ccgccgcct gccgtgcag 
      ccgcccccg gagccagtc tccacagcc atcgtacct 
      gcccccgta caaaacgcc aaagtacat aataataag 
      ttgcacgag aaaaacggc cacatcccc aatgggacg 
      atagccaac ggtgtgcac gcgaccaaa tcacatcat 
      agaaagtct gtcgataaa tgtaaagaa cacgacgaa 
      cacaacagg cattcggat aagcacata gatagacaa 
      agacaagtt aatcattcg cacagtaat ggaacggcg 
      attagtgcc ggcgttgaa gaaatcacg tcgacgacg 
      gcgacggcc acggttcaa gaacatgaa ccgagaccg 
      acgaggcga agagaaaag aaggaacag gataaggag 
      agagaagcg gccgagtat ctgcaaaag ctcgaggcc 
      gacgctaaa aggctgcgg gcggaccta caaagcagc 
      agagccagc gagcaagaa ctcaggctg caggttgcc 
      gcgctcacc acgagcgag aaggtaatc aagggcgag 
      ctgacagcg atgcaacgc gaaatcgaa gacgtgcaa 
      cagcgttat caaagtgtt gcaagttcc cgcgcttcc 
      gataaacag acgatcagc aacttggaa cgtcgagtg 
      gctgaagaa aggcgagca agatgcaat ctagaatcg 
      caactaaat caagaacgt aaaagtaga aaacaagag 
      gaggcccga gcggctcaa gtggtggca cagtcatcg 
      cgcggcgag tgcacggaa gcgtgccga gcgcgtcgt 
      agagaatta gaagccgaa ttgagcgag tgtcggaca 
      acgaagcat tgggccgaa gaacggtac tccgcgctg 
      gaacgcgaa aatgccgcc ctagtcgat cagctccgt 
      gatatggac atactcatg accgccctc acggcaatg 
      caggagaaa aacgcccat ttagaaaat tcgttatcg 
      gccgagacg cgcatcaag ctcgacctc ttcagcgcc 
      ctgggcgag gcgaagcga caactcgaa atcagagag 
      agctccaac aggacgcaa gaacgcgag atcgaagag 
      ctgaagagc aaaatcgcg caagtgttg gctgtgatg 
      ccgaacgac agtttcggc ggtccgacg gtggcgacg 
      agcaacatg tcgcgagtc cggctggcc gagagtccg 
      cccggttcg accctggac ccgaacgcc acggcctac 
      acccctaag agttccctt gtcgcttcc accgaagcc 
      tagttgacc tcttaacgc cccacctga ctccgtact 
      gaacccggt atcgactgc tattgttta ttgttgcgt 
      taagtcgcg tatgtttcg tcatagtct cgtttagta 
      gcttattta atgtgatat aatttatga aaacggtac 
      cttgatcat gaggcaacc cctcctcta ttttgtttg 
      tttatataa tttaagcat aaaggtact gagtttttc 
      tgacagttg ggtcgcgcg attggcaga ctaatgatc 
      tctattttg ttgattttt ttctttgat tatccgttc 
      tggtggaaa tcaggggat ttggataga atcacgtcc 
      agtttattt tgttgttga gcattgcat tgagaataa 
      catactaac acaaggata gaagttttg tttcttttg 
      aaagtgccg tttaacagc tgtctggga tttttgttt 
      tacgtgttt aacaaaagg tgataattt ttggaaacg 
      tcaaaaatg tacttaata tattttttt aattgtttt 
      tagttgaga caacgtttg ttttctttt tattacaaa 
      tacagtaat gattctttt ttaaagttt tttctttat 
      ttggttcct ttaattttg tagttttga ggcgaacaa 
      ctatgtcgg ggattccct aagacgatc tgttattta 
      tttgccata tttattttt cttttgtat actactggt 
      agtatttat ttttttact gttgcaaac ttttatttc 
      gtaaataaa cactattac agcttttcc tgtttcttt 
      gacgtgttt ttatggttt gacgttttt ctacgtaga 
      attaagaat ccttgccgt tctattctt tttactaaa 
      ttctcacgg tataatagg taggcagta catgttatg 
      aagttttgt caactcata aagttggta tgatggtct 
      ttctgctct atgtgtttt gaaaatgac aattcgaga 
      aaaaattaa ccacaaaat aaatattgg ctcacagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY