Status
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Gene information
General information
TC034116
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005568,TC005566 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11207777..11241418
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034116
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034116 RA
      MHLTANSQT ATGTAASPE GSSVINDTY TKMTSDILA 
ERTLNDFLS EHPGELIRT GSPLFVCTV LPPHWRSNK 
TLPVAFKVV ALGDVGDGT VVTVKAGND ENYCAELRN 
STAVMKNQV AKFNDLRFV GRSGRGKSF TLTIMVSTS 
PPQVATYNK AIKVTVDGP REPRSKTRQ QQQFHFAFG 
QRPFPFAAS DPLSGFRMP PIGNCNNMP QFGLSSTNS 
HWGYGAAGA YSPYFTPST LGSCAAPTA SQFNTPALG 
FSGSTPDQT STQDAFGST SNVTSLLPD TSTTDLDQH 
LGLVSSQNH TNHSQSSTH TSSLLVPRY STNHSDFGL 
SGPRSLSDN SSAAESPVQ DDILSTQST LGVNHVNNT 
NFPLHQNMT SSSYPSSNC NNSIYPVLP ASLLYSQLY 
SAANQSHNF HSLHSHTTQ SHHNDLQTV MDQLSTSNQ 
RQMNGSTDL LLSNNGTCA AAAARQDDA RLTSNGAQR 
GPGQNSDAV VWRPY
    • >TC034116 RB
      MHLTANSQT ATGTAASPE GSSVINDTY TKMTSDILA 
ERTLNDFLS EHPGELIRT GSPLFVCTV LPPHWRSNK 
TLPVAFKVV ALGDVGDGT VVTVKAGND ENYCAELRN 
STAVMKNQV AKFNDLRFV GRSGRGKSF TLTIMVSTS 
PPQVATYNK AIKVTVDGP REPRSKTML SLLGQQQQF 
HFAFGQRPF PFAASDPLS GFRMPPIGN CNNMPQFGL 
SSTNSHWGY GAAGAYSPY FTPSTLGSC AAPTASQFN 
TPALGFSGS TPDQTSTQD AFGSTSNVT SLLPDTSTT 
DLDQHLGLV SSQNHTNHS QSSTHTSSL LVPRYSTNH 
SDFGLSGPR SLSDNSSAA ESPVQDDIL STQSTLGVN 
HVNNTNFPL HQNMTSSSY PSSNCNNSI YPVLPASLL 
YSQLYSAAN QSHNFHSLH SHTTQSHHN DLQTVMDQL 
STSNQRQMN GSTDLLLSN NGTCAAAAA RQDDARLTS 
NGAQRGPGQ NSDAVVWRP Y
    CDS sequence
    • >TC034116 RA
      atgcacttg acggccaac tcgcagacg gccacgggg 
acggcggcc tcgccggaa ggctccagc gtcatcaac 
gacacctac accaagatg acgtcggac atcctcgcc 
gagcgcacc ctcaacgac ttcctgtcg gagcacccg 
ggggagctc atccgcacc ggcagcccg ctcttcgtc 
tgcacggtg ctgccgccg cactggcgc tccaacaag 
acgctgccg gtggccttc aaggtggtg gccctcggc 
gacgtgggc gacggcacc gtcgtcacc gtcaaggcg 
ggcaacgac gagaactac tgcgccgag ctgaggaac 
tccacggcg gtcatgaag aaccaggtg gccaagttc 
aacgatttg aggttcgtc ggaaggagc ggaagaggg 
aagagtttc acgctgacc attatggtt tcgacgagt 
ccgccccaa gtcgctacc tacaataag gcgatcaag 
gtgactgtg gatgggccc agggagccg cgatcgaag 
acaaggcag cagcagcaa tttcatttc gctttcggg 
caacggccc tttcctttc gccgcctcg gaccctttg 
tcaggcttc aggatgccg ccgataggg aattgcaac 
aatatgccc caatttggg ttgagcagc actaattcg 
cactggggg tatggggcc gcaggggcg tattcgcct 
tacttcacc cccagtacg ttggggtcg tgtgcggcg 
cccacggcg tcccagttt aataccccg gctttgggg 
ttctcgggg agtaccccg gatcagacg tccacgcaa 
gatgctttc gggtcaacg tccaacgtc acctcatta 
ctccctgac acctcgacg acggacctg gaccagcac 
ctaggcctc gtctcttcg caaaaccac acgaaccac 
tcccagagc agcacccac acctcgtcc cttctagtc 
cctcgctac tccaccaac cactcggac ttcggcctt 
tcgggccct cgctccctc tcggacaac tccagtgct 
gcggagagc cccgtccag gacgacatc ctctccacc 
caaagcacc ctcggcgtg aaccacgtc aacaacacc 
aacttcccg ctgcaccag aacatgacg tcgtcgtcg 
taccccagc agcaactgc aacaactca atctatccc 
gtccttcct gcgagtctt ctctactcg cagctgtat 
tcagcggcc aaccaaagc cacaacttc cactcgctg 
cactcgcac acgacgcaa agccaccac aatgacctg 
cagacggtc atggaccag ctgtccacc agcaaccag 
cggcagatg aacggctcc acggacctg cttctgagc 
aataatggg acgtgtgcg gcggcggcg gcgagacag 
gacgatgcc aggttgacg agcaacggg gcccagagg 
ggccctgga cagaacagt gacgcggtg gtgtggagg 
ccgtattga
    • >TC034116 RB
      atgcacttg acggccaac tcgcagacg gccacgggg 
acggcggcc tcgccggaa ggctccagc gtcatcaac 
gacacctac accaagatg acgtcggac atcctcgcc 
gagcgcacc ctcaacgac ttcctgtcg gagcacccg 
ggggagctc atccgcacc ggcagcccg ctcttcgtc 
tgcacggtg ctgccgccg cactggcgc tccaacaag 
acgctgccg gtggccttc aaggtggtg gccctcggc 
gacgtgggc gacggcacc gtcgtcacc gtcaaggcg 
ggcaacgac gagaactac tgcgccgag ctgaggaac 
tccacggcg gtcatgaag aaccaggtg gccaagttc 
aacgatttg aggttcgtc ggaaggagc ggaagaggg 
aagagtttc acgctgacc attatggtt tcgacgagt 
ccgccccaa gtcgctacc tacaataag gcgatcaag 
gtgactgtg gatgggccc agggagccg cgatcgaag 
acaatgctt tctctttta gggcagcag cagcaattt 
catttcgct ttcgggcaa cggcccttt cctttcgcc 
gcctcggac cctttgtca ggcttcagg atgccgccg 
atagggaat tgcaacaat atgccccaa tttgggttg 
agcagcact aattcgcac tgggggtat ggggccgca 
ggggcgtat tcgccttac ttcaccccc agtacgttg 
gggtcgtgt gcggcgccc acggcgtcc cagtttaat 
accccggct ttggggttc tcggggagt accccggat 
cagacgtcc acgcaagat gctttcggg tcaacgtcc 
aacgtcacc tcattactc cctgacacc tcgacgacg 
gacctggac cagcaccta ggcctcgtc tcttcgcaa 
aaccacacg aaccactcc cagagcagc acccacacc 
tcgtccctt ctagtccct cgctactcc accaaccac 
tcggacttc ggcctttcg ggccctcgc tccctctcg 
gacaactcc agtgctgcg gagagcccc gtccaggac 
gacatcctc tccacccaa agcaccctc ggcgtgaac 
cacgtcaac aacaccaac ttcccgctg caccagaac 
atgacgtcg tcgtcgtac cccagcagc aactgcaac 
aactcaatc tatcccgtc cttcctgcg agtcttctc 
tactcgcag ctgtattca gcggccaac caaagccac 
aacttccac tcgctgcac tcgcacacg acgcaaagc 
caccacaat gacctgcag acggtcatg gaccagctg 
tccaccagc aaccagcgg cagatgaac ggctccacg 
gacctgctt ctgagcaat aatgggacg tgtgcggcg 
gcggcggcg agacaggac gatgccagg ttgacgagc 
aacggggcc cagaggggc cctggacag aacagtgac 
gcggtggtg tggaggccg tattga
    mRNA sequence
    • >TC034116 RA
      gtcttcaga tgcaaccaa aaatactaa ctgcacata 
ccatctgaa cagcgtggt ggaatgtaa accatttct 
agcgcaaaa ccggccacg tgcccatta gcagccctt 
aattttttc ctcttccct catctttgg cacaccgtc 
attatcacc gttccacac acagcccgt aaactgtgc 
ggtctgtgt gaacctcta cgccgtgtt gatccggga 
gctctgcgt ggatgtcgg cgtgctccg gttagccgc 
caccgaaga caaaacaca tgacaataa cacagcccc 
cgtcagcat atgtgacgc ctgcgccgc cttctccaa 
catgctgat aaaccgcgt tcttgtcac tcctacgcc 
aatttatat acttcgctc ctttattac cgccaggcc 
cggcccaat ttgaccgca aacacgacc ctctccctc 
ccacgcatt cgaatcccg ctgcgttcg tcccgctct 
agcacgtac ccctttatg ttagcttct gttttaatc 
tggaattcg ggcgtcctt gtctggcgc cgggggggc 
ccatggcgg cgtctctct cggcctccc ctgataacc 
aaagggtta tcaagcgca agagtgcta ctgcttttg 
gacgcaaac cgcgacgtg tctccggtt ggctgtcct 
tgtccctcg gcggggctg tcggaggcg ccggcggcg 
acgtaggtc ctcgcatgt gttttcggt agttaatca 
acaacacac tgttaaagc ctttagttt ggtcaaatt 
cgtgataga acgaaacag atgaagcgg ttcggcgtc 
tcggagcct cctctggaa gagttcctt ttggcggcg 
gtgccgtag cgtgtgtgg tgcgtgcgt gtgcagttg 
tccgacgcc cgcgacgcg ttccacacg gcgtcccga 
cgccgcgat gtaaaagtg cgccccatt tttccaccc 
caccaagtt tcattcaga aaaagtgtt gacattacg 
tcatgtgtt gcttggcaa ccgacggat gcgtgtcac 
gcggcgcct ttttttgac atgggggcc cctctggag 
acttgatgt tcggcgagt ggaacccca cgatgcact 
tgacggcca actcgcaga cggccacgg ggacggcgg 
cctcgccgg aaggctcca gcgtcatca acgacacct 
acaccaaga tgacgtcgg acatcctcg ccgagcgca 
ccctcaacg acttcctgt cggagcacc cgggggagc 
tcatccgca ccggcagcc cgctcttcg tctgcacgg 
tgctgccgc cgcactggc gctccaaca agacgctgc 
cggtggcct tcaaggtgg tggccctcg gcgacgtgg 
gcgacggca ccgtcgtca ccgtcaagg cgggcaacg 
acgagaact actgcgccg agctgagga actccacgg 
cggtcatga agaaccagg tggccaagt tcaacgatt 
tgaggttcg tcggaagga gcggaagag ggaagagtt 
tcacgctga ccattatgg tttcgacga gtccgcccc 
aagtcgcta cctacaata aggcgatca aggtgactg 
tggatgggc ccagggagc cgcgatcga agacaaggc 
agcagcagc aatttcatt tcgctttcg ggcaacggc 
cctttcctt tcgccgcct cggaccctt tgtcaggct 
tcaggatgc cgccgatag ggaattgca acaatatgc 
cccaatttg ggttgagca gcactaatt cgcactggg 
ggtatgggg ccgcagggg cgtattcgc cttacttca 
cccccagta cgttggggt cgtgtgcgg cgcccacgg 
cgtcccagt ttaataccc cggctttgg ggttctcgg 
ggagtaccc cggatcaga cgtccacgc aagatgctt 
tcgggtcaa cgtccaacg tcacctcat tactccctg 
acacctcga cgacggacc tggaccagc acctaggcc 
tcgtctctt cgcaaaacc acacgaacc actcccaga 
gcagcaccc acacctcgt cccttctag tccctcgct 
actccacca accactcgg acttcggcc tttcgggcc 
ctcgctccc tctcggaca actccagtg ctgcggaga 
gccccgtcc aggacgaca tcctctcca cccaaagca 
ccctcggcg tgaaccacg tcaacaaca ccaacttcc 
cgctgcacc agaacatga cgtcgtcgt cgtacccca 
gcagcaact gcaacaact caatctatc ccgtccttc 
ctgcgagtc ttctctact cgcagctgt attcagcgg 
ccaaccaaa gccacaact tccactcgc tgcactcgc 
acacgacgc aaagccacc acaatgacc tgcagacgg 
tcatggacc agctgtcca ccagcaacc agcggcaga 
tgaacggct ccacggacc tgcttctga gcaataatg 
ggacgtgtg cggcggcgg cggcgagac aggacgatg 
ccaggttga cgagcaacg gggcccaga ggggccctg 
gacagaaca gtgacgcgg tggtgtgga ggccgtatt 
gagtgaccg aggaaacga tgtgatttt tgtgcaata 
ttgttgact agttttggg gttgttggg actttgagg 
ggtgtgttt ccgccaagt ttcaaactt ttcgcattt 
gaactcaac gggaaacta taggtttag gtaatctac 
caggttttt tttactttg ttacgctcg ttaatctaa 
taaaaagta acaatgttg
    • >TC034116 RB
      gtcttcaga tgcaaccaa aaatactaa ctgcacata 
ccatctgaa cagcgtggt ggaatgtaa accatttct 
agcgcaaaa ccggccacg tgcccatta gcagccctt 
aattttttc ctcttccct catctttgg cacaccgtc 
attatcacc gttccacac acagcccgt aaactgtgc 
ggtctgtgt gaacctcta cgccgtgtt gatccggga 
gctctgcgt ggatgtcgg cgtgctccg gttagccgc 
caccgaaga caaaacaca tgacaataa cacagcccc 
cgtcagcat atgtgacgc ctgcgccgc cttctccaa 
catgctgat aaaccgcgt tcttgtcac tcctacgcc 
aatttatat acttcgctc ctttattac cgccaggcc 
cggcccaat ttgaccgca aacacgacc ctctccctc 
ccacgcatt cgaatcccg ctgcgttcg tcccgctct 
agcacgtac ccctttatg ttagcttct gttttaatc 
tggaattcg ggcgtcctt gtctggcgc cgggggggc 
ccatggcgg cgtctctct cggcctccc ctgataacc 
aaagggtta tcaagcgca agagtgcta ctgcttttg 
gacgcaaac cgcgacgtg tctccggtt ggctgtcct 
tgtccctcg gcggggctg tcggaggcg ccggcggcg 
acgtaggtc ctcgcatgt gttttcggt agttaatca 
acaacacac tgttaaagc ctttagttt ggtcaaatt 
cgtgataga acgaaacag atgaagcgg ttcggcgtc 
tcggagcct cctctggaa gagttcctt ttggcggcg 
gtgccgtag cgtgtgtgg tgcgtgcgt gtgcagttg 
tccgacgcc cgcgacgcg ttccacacg gcgtcccga 
cgccgcgat gtaaaagtg cgccccatt tttccaccc 
caccaagtt tcattcaga aaaagtgtt gacattacg 
tcatgtgtt gcttggcaa ccgacggat gcgtgtcac 
gcggcgcct ttttttgac atgggggcc cctctggag 
acttgatgt tcggcgagt ggaacccca cgatgcact 
tgacggcca actcgcaga cggccacgg ggacggcgg 
cctcgccgg aaggctcca gcgtcatca acgacacct 
acaccaaga tgacgtcgg acatcctcg ccgagcgca 
ccctcaacg acttcctgt cggagcacc cgggggagc 
tcatccgca ccggcagcc cgctcttcg tctgcacgg 
tgctgccgc cgcactggc gctccaaca agacgctgc 
cggtggcct tcaaggtgg tggccctcg gcgacgtgg 
gcgacggca ccgtcgtca ccgtcaagg cgggcaacg 
acgagaact actgcgccg agctgagga actccacgg 
cggtcatga agaaccagg tggccaagt tcaacgatt 
tgaggttcg tcggaagga gcggaagag ggaagagtt 
tcacgctga ccattatgg tttcgacga gtccgcccc 
aagtcgcta cctacaata aggcgatca aggtgactg 
tggatgggc ccagggagc cgcgatcga agacaatgc 
tttctcttt tagggcagc agcagcaat ttcatttcg 
ctttcgggc aacggccct ttcctttcg ccgcctcgg 
accctttgt caggcttca ggatgccgc cgataggga 
attgcaaca atatgcccc aatttgggt tgagcagca 
ctaattcgc actgggggt atggggccg caggggcgt 
attcgcctt acttcaccc ccagtacgt tggggtcgt 
gtgcggcgc ccacggcgt cccagttta ataccccgg 
ctttggggt tctcgggga gtaccccgg atcagacgt 
ccacgcaag atgctttcg ggtcaacgt ccaacgtca 
cctcattac tccctgaca cctcgacga cggacctgg 
accagcacc taggcctcg tctcttcgc aaaaccaca 
cgaaccact cccagagca gcacccaca cctcgtccc 
ttctagtcc ctcgctact ccaccaacc actcggact 
tcggccttt cgggccctc gctccctct cggacaact 
ccagtgctg cggagagcc ccgtccagg acgacatcc 
tctccaccc aaagcaccc tcggcgtga accacgtca 
acaacacca acttcccgc tgcaccaga acatgacgt 
cgtcgtcgt accccagca gcaactgca acaactcaa 
tctatcccg tccttcctg cgagtcttc tctactcgc 
agctgtatt cagcggcca accaaagcc acaacttcc 
actcgctgc actcgcaca cgacgcaaa gccaccaca 
atgacctgc agacggtca tggaccagc tgtccacca 
gcaaccagc ggcagatga acggctcca cggacctgc 
ttctgagca ataatggga cgtgtgcgg cggcggcgg 
cgagacagg acgatgcca ggttgacga gcaacgggg 
cccagaggg gccctggac agaacagtg acgcggtgg 
tgtggaggc cgtattgag tgaccgagg aaacgatgt 
gatttttgt gcaatattg ttgactagt tttggggtt 
gttgggact ttgaggggt gtgtttccg ccaagtttc 
aaacttttc gcatttgaa ctcaacggg aaactatag 
gtttaggta atctaccag gtttttttt actttgtta 
cgctcgtta atctaataa aaagtaaca atgttg
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