Status
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Gene information
General information
TC034116
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005568,TC005566
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11207777..11241418
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034116
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034116 RA
      MHLTANSQT ATGTAASPE GSSVINDTY TKMTSDILA 
      ERTLNDFLS EHPGELIRT GSPLFVCTV LPPHWRSNK 
      TLPVAFKVV ALGDVGDGT VVTVKAGND ENYCAELRN 
      STAVMKNQV AKFNDLRFV GRSGRGKSF TLTIMVSTS 
      PPQVATYNK AIKVTVDGP REPRSKTRQ QQQFHFAFG 
      QRPFPFAAS DPLSGFRMP PIGNCNNMP QFGLSSTNS 
      HWGYGAAGA YSPYFTPST LGSCAAPTA SQFNTPALG 
      FSGSTPDQT STQDAFGST SNVTSLLPD TSTTDLDQH 
      LGLVSSQNH TNHSQSSTH TSSLLVPRY STNHSDFGL 
      SGPRSLSDN SSAAESPVQ DDILSTQST LGVNHVNNT 
      NFPLHQNMT SSSYPSSNC NNSIYPVLP ASLLYSQLY 
      SAANQSHNF HSLHSHTTQ SHHNDLQTV MDQLSTSNQ 
      RQMNGSTDL LLSNNGTCA AAAARQDDA RLTSNGAQR 
      GPGQNSDAV VWRPY
    • >TC034116 RB
      MHLTANSQT ATGTAASPE GSSVINDTY TKMTSDILA 
      ERTLNDFLS EHPGELIRT GSPLFVCTV LPPHWRSNK 
      TLPVAFKVV ALGDVGDGT VVTVKAGND ENYCAELRN 
      STAVMKNQV AKFNDLRFV GRSGRGKSF TLTIMVSTS 
      PPQVATYNK AIKVTVDGP REPRSKTML SLLGQQQQF 
      HFAFGQRPF PFAASDPLS GFRMPPIGN CNNMPQFGL 
      SSTNSHWGY GAAGAYSPY FTPSTLGSC AAPTASQFN 
      TPALGFSGS TPDQTSTQD AFGSTSNVT SLLPDTSTT 
      DLDQHLGLV SSQNHTNHS QSSTHTSSL LVPRYSTNH 
      SDFGLSGPR SLSDNSSAA ESPVQDDIL STQSTLGVN 
      HVNNTNFPL HQNMTSSSY PSSNCNNSI YPVLPASLL 
      YSQLYSAAN QSHNFHSLH SHTTQSHHN DLQTVMDQL 
      STSNQRQMN GSTDLLLSN NGTCAAAAA RQDDARLTS 
      NGAQRGPGQ NSDAVVWRP Y
    CDS sequence
    • >TC034116 RA
      atgcacttg acggccaac tcgcagacg gccacgggg 
      acggcggcc tcgccggaa ggctccagc gtcatcaac 
      gacacctac accaagatg acgtcggac atcctcgcc 
      gagcgcacc ctcaacgac ttcctgtcg gagcacccg 
      ggggagctc atccgcacc ggcagcccg ctcttcgtc 
      tgcacggtg ctgccgccg cactggcgc tccaacaag 
      acgctgccg gtggccttc aaggtggtg gccctcggc 
      gacgtgggc gacggcacc gtcgtcacc gtcaaggcg 
      ggcaacgac gagaactac tgcgccgag ctgaggaac 
      tccacggcg gtcatgaag aaccaggtg gccaagttc 
      aacgatttg aggttcgtc ggaaggagc ggaagaggg 
      aagagtttc acgctgacc attatggtt tcgacgagt 
      ccgccccaa gtcgctacc tacaataag gcgatcaag 
      gtgactgtg gatgggccc agggagccg cgatcgaag 
      acaaggcag cagcagcaa tttcatttc gctttcggg 
      caacggccc tttcctttc gccgcctcg gaccctttg 
      tcaggcttc aggatgccg ccgataggg aattgcaac 
      aatatgccc caatttggg ttgagcagc actaattcg 
      cactggggg tatggggcc gcaggggcg tattcgcct 
      tacttcacc cccagtacg ttggggtcg tgtgcggcg 
      cccacggcg tcccagttt aataccccg gctttgggg 
      ttctcgggg agtaccccg gatcagacg tccacgcaa 
      gatgctttc gggtcaacg tccaacgtc acctcatta 
      ctccctgac acctcgacg acggacctg gaccagcac 
      ctaggcctc gtctcttcg caaaaccac acgaaccac 
      tcccagagc agcacccac acctcgtcc cttctagtc 
      cctcgctac tccaccaac cactcggac ttcggcctt 
      tcgggccct cgctccctc tcggacaac tccagtgct 
      gcggagagc cccgtccag gacgacatc ctctccacc 
      caaagcacc ctcggcgtg aaccacgtc aacaacacc 
      aacttcccg ctgcaccag aacatgacg tcgtcgtcg 
      taccccagc agcaactgc aacaactca atctatccc 
      gtccttcct gcgagtctt ctctactcg cagctgtat 
      tcagcggcc aaccaaagc cacaacttc cactcgctg 
      cactcgcac acgacgcaa agccaccac aatgacctg 
      cagacggtc atggaccag ctgtccacc agcaaccag 
      cggcagatg aacggctcc acggacctg cttctgagc 
      aataatggg acgtgtgcg gcggcggcg gcgagacag 
      gacgatgcc aggttgacg agcaacggg gcccagagg 
      ggccctgga cagaacagt gacgcggtg gtgtggagg 
      ccgtattga
    • >TC034116 RB
      atgcacttg acggccaac tcgcagacg gccacgggg 
      acggcggcc tcgccggaa ggctccagc gtcatcaac 
      gacacctac accaagatg acgtcggac atcctcgcc 
      gagcgcacc ctcaacgac ttcctgtcg gagcacccg 
      ggggagctc atccgcacc ggcagcccg ctcttcgtc 
      tgcacggtg ctgccgccg cactggcgc tccaacaag 
      acgctgccg gtggccttc aaggtggtg gccctcggc 
      gacgtgggc gacggcacc gtcgtcacc gtcaaggcg 
      ggcaacgac gagaactac tgcgccgag ctgaggaac 
      tccacggcg gtcatgaag aaccaggtg gccaagttc 
      aacgatttg aggttcgtc ggaaggagc ggaagaggg 
      aagagtttc acgctgacc attatggtt tcgacgagt 
      ccgccccaa gtcgctacc tacaataag gcgatcaag 
      gtgactgtg gatgggccc agggagccg cgatcgaag 
      acaatgctt tctctttta gggcagcag cagcaattt 
      catttcgct ttcgggcaa cggcccttt cctttcgcc 
      gcctcggac cctttgtca ggcttcagg atgccgccg 
      atagggaat tgcaacaat atgccccaa tttgggttg 
      agcagcact aattcgcac tgggggtat ggggccgca 
      ggggcgtat tcgccttac ttcaccccc agtacgttg 
      gggtcgtgt gcggcgccc acggcgtcc cagtttaat 
      accccggct ttggggttc tcggggagt accccggat 
      cagacgtcc acgcaagat gctttcggg tcaacgtcc 
      aacgtcacc tcattactc cctgacacc tcgacgacg 
      gacctggac cagcaccta ggcctcgtc tcttcgcaa 
      aaccacacg aaccactcc cagagcagc acccacacc 
      tcgtccctt ctagtccct cgctactcc accaaccac 
      tcggacttc ggcctttcg ggccctcgc tccctctcg 
      gacaactcc agtgctgcg gagagcccc gtccaggac 
      gacatcctc tccacccaa agcaccctc ggcgtgaac 
      cacgtcaac aacaccaac ttcccgctg caccagaac 
      atgacgtcg tcgtcgtac cccagcagc aactgcaac 
      aactcaatc tatcccgtc cttcctgcg agtcttctc 
      tactcgcag ctgtattca gcggccaac caaagccac 
      aacttccac tcgctgcac tcgcacacg acgcaaagc 
      caccacaat gacctgcag acggtcatg gaccagctg 
      tccaccagc aaccagcgg cagatgaac ggctccacg 
      gacctgctt ctgagcaat aatgggacg tgtgcggcg 
      gcggcggcg agacaggac gatgccagg ttgacgagc 
      aacggggcc cagaggggc cctggacag aacagtgac 
      gcggtggtg tggaggccg tattga
    mRNA sequence
    • >TC034116 RA
      gtcttcaga tgcaaccaa aaatactaa ctgcacata 
      ccatctgaa cagcgtggt ggaatgtaa accatttct 
      agcgcaaaa ccggccacg tgcccatta gcagccctt 
      aattttttc ctcttccct catctttgg cacaccgtc 
      attatcacc gttccacac acagcccgt aaactgtgc 
      ggtctgtgt gaacctcta cgccgtgtt gatccggga 
      gctctgcgt ggatgtcgg cgtgctccg gttagccgc 
      caccgaaga caaaacaca tgacaataa cacagcccc 
      cgtcagcat atgtgacgc ctgcgccgc cttctccaa 
      catgctgat aaaccgcgt tcttgtcac tcctacgcc 
      aatttatat acttcgctc ctttattac cgccaggcc 
      cggcccaat ttgaccgca aacacgacc ctctccctc 
      ccacgcatt cgaatcccg ctgcgttcg tcccgctct 
      agcacgtac ccctttatg ttagcttct gttttaatc 
      tggaattcg ggcgtcctt gtctggcgc cgggggggc 
      ccatggcgg cgtctctct cggcctccc ctgataacc 
      aaagggtta tcaagcgca agagtgcta ctgcttttg 
      gacgcaaac cgcgacgtg tctccggtt ggctgtcct 
      tgtccctcg gcggggctg tcggaggcg ccggcggcg 
      acgtaggtc ctcgcatgt gttttcggt agttaatca 
      acaacacac tgttaaagc ctttagttt ggtcaaatt 
      cgtgataga acgaaacag atgaagcgg ttcggcgtc 
      tcggagcct cctctggaa gagttcctt ttggcggcg 
      gtgccgtag cgtgtgtgg tgcgtgcgt gtgcagttg 
      tccgacgcc cgcgacgcg ttccacacg gcgtcccga 
      cgccgcgat gtaaaagtg cgccccatt tttccaccc 
      caccaagtt tcattcaga aaaagtgtt gacattacg 
      tcatgtgtt gcttggcaa ccgacggat gcgtgtcac 
      gcggcgcct ttttttgac atgggggcc cctctggag 
      acttgatgt tcggcgagt ggaacccca cgatgcact 
      tgacggcca actcgcaga cggccacgg ggacggcgg 
      cctcgccgg aaggctcca gcgtcatca acgacacct 
      acaccaaga tgacgtcgg acatcctcg ccgagcgca 
      ccctcaacg acttcctgt cggagcacc cgggggagc 
      tcatccgca ccggcagcc cgctcttcg tctgcacgg 
      tgctgccgc cgcactggc gctccaaca agacgctgc 
      cggtggcct tcaaggtgg tggccctcg gcgacgtgg 
      gcgacggca ccgtcgtca ccgtcaagg cgggcaacg 
      acgagaact actgcgccg agctgagga actccacgg 
      cggtcatga agaaccagg tggccaagt tcaacgatt 
      tgaggttcg tcggaagga gcggaagag ggaagagtt 
      tcacgctga ccattatgg tttcgacga gtccgcccc 
      aagtcgcta cctacaata aggcgatca aggtgactg 
      tggatgggc ccagggagc cgcgatcga agacaaggc 
      agcagcagc aatttcatt tcgctttcg ggcaacggc 
      cctttcctt tcgccgcct cggaccctt tgtcaggct 
      tcaggatgc cgccgatag ggaattgca acaatatgc 
      cccaatttg ggttgagca gcactaatt cgcactggg 
      ggtatgggg ccgcagggg cgtattcgc cttacttca 
      cccccagta cgttggggt cgtgtgcgg cgcccacgg 
      cgtcccagt ttaataccc cggctttgg ggttctcgg 
      ggagtaccc cggatcaga cgtccacgc aagatgctt 
      tcgggtcaa cgtccaacg tcacctcat tactccctg 
      acacctcga cgacggacc tggaccagc acctaggcc 
      tcgtctctt cgcaaaacc acacgaacc actcccaga 
      gcagcaccc acacctcgt cccttctag tccctcgct 
      actccacca accactcgg acttcggcc tttcgggcc 
      ctcgctccc tctcggaca actccagtg ctgcggaga 
      gccccgtcc aggacgaca tcctctcca cccaaagca 
      ccctcggcg tgaaccacg tcaacaaca ccaacttcc 
      cgctgcacc agaacatga cgtcgtcgt cgtacccca 
      gcagcaact gcaacaact caatctatc ccgtccttc 
      ctgcgagtc ttctctact cgcagctgt attcagcgg 
      ccaaccaaa gccacaact tccactcgc tgcactcgc 
      acacgacgc aaagccacc acaatgacc tgcagacgg 
      tcatggacc agctgtcca ccagcaacc agcggcaga 
      tgaacggct ccacggacc tgcttctga gcaataatg 
      ggacgtgtg cggcggcgg cggcgagac aggacgatg 
      ccaggttga cgagcaacg gggcccaga ggggccctg 
      gacagaaca gtgacgcgg tggtgtgga ggccgtatt 
      gagtgaccg aggaaacga tgtgatttt tgtgcaata 
      ttgttgact agttttggg gttgttggg actttgagg 
      ggtgtgttt ccgccaagt ttcaaactt ttcgcattt 
      gaactcaac gggaaacta taggtttag gtaatctac 
      caggttttt tttactttg ttacgctcg ttaatctaa 
      taaaaagta acaatgttg
    • >TC034116 RB
      gtcttcaga tgcaaccaa aaatactaa ctgcacata 
      ccatctgaa cagcgtggt ggaatgtaa accatttct 
      agcgcaaaa ccggccacg tgcccatta gcagccctt 
      aattttttc ctcttccct catctttgg cacaccgtc 
      attatcacc gttccacac acagcccgt aaactgtgc 
      ggtctgtgt gaacctcta cgccgtgtt gatccggga 
      gctctgcgt ggatgtcgg cgtgctccg gttagccgc 
      caccgaaga caaaacaca tgacaataa cacagcccc 
      cgtcagcat atgtgacgc ctgcgccgc cttctccaa 
      catgctgat aaaccgcgt tcttgtcac tcctacgcc 
      aatttatat acttcgctc ctttattac cgccaggcc 
      cggcccaat ttgaccgca aacacgacc ctctccctc 
      ccacgcatt cgaatcccg ctgcgttcg tcccgctct 
      agcacgtac ccctttatg ttagcttct gttttaatc 
      tggaattcg ggcgtcctt gtctggcgc cgggggggc 
      ccatggcgg cgtctctct cggcctccc ctgataacc 
      aaagggtta tcaagcgca agagtgcta ctgcttttg 
      gacgcaaac cgcgacgtg tctccggtt ggctgtcct 
      tgtccctcg gcggggctg tcggaggcg ccggcggcg 
      acgtaggtc ctcgcatgt gttttcggt agttaatca 
      acaacacac tgttaaagc ctttagttt ggtcaaatt 
      cgtgataga acgaaacag atgaagcgg ttcggcgtc 
      tcggagcct cctctggaa gagttcctt ttggcggcg 
      gtgccgtag cgtgtgtgg tgcgtgcgt gtgcagttg 
      tccgacgcc cgcgacgcg ttccacacg gcgtcccga 
      cgccgcgat gtaaaagtg cgccccatt tttccaccc 
      caccaagtt tcattcaga aaaagtgtt gacattacg 
      tcatgtgtt gcttggcaa ccgacggat gcgtgtcac 
      gcggcgcct ttttttgac atgggggcc cctctggag 
      acttgatgt tcggcgagt ggaacccca cgatgcact 
      tgacggcca actcgcaga cggccacgg ggacggcgg 
      cctcgccgg aaggctcca gcgtcatca acgacacct 
      acaccaaga tgacgtcgg acatcctcg ccgagcgca 
      ccctcaacg acttcctgt cggagcacc cgggggagc 
      tcatccgca ccggcagcc cgctcttcg tctgcacgg 
      tgctgccgc cgcactggc gctccaaca agacgctgc 
      cggtggcct tcaaggtgg tggccctcg gcgacgtgg 
      gcgacggca ccgtcgtca ccgtcaagg cgggcaacg 
      acgagaact actgcgccg agctgagga actccacgg 
      cggtcatga agaaccagg tggccaagt tcaacgatt 
      tgaggttcg tcggaagga gcggaagag ggaagagtt 
      tcacgctga ccattatgg tttcgacga gtccgcccc 
      aagtcgcta cctacaata aggcgatca aggtgactg 
      tggatgggc ccagggagc cgcgatcga agacaatgc 
      tttctcttt tagggcagc agcagcaat ttcatttcg 
      ctttcgggc aacggccct ttcctttcg ccgcctcgg 
      accctttgt caggcttca ggatgccgc cgataggga 
      attgcaaca atatgcccc aatttgggt tgagcagca 
      ctaattcgc actgggggt atggggccg caggggcgt 
      attcgcctt acttcaccc ccagtacgt tggggtcgt 
      gtgcggcgc ccacggcgt cccagttta ataccccgg 
      ctttggggt tctcgggga gtaccccgg atcagacgt 
      ccacgcaag atgctttcg ggtcaacgt ccaacgtca 
      cctcattac tccctgaca cctcgacga cggacctgg 
      accagcacc taggcctcg tctcttcgc aaaaccaca 
      cgaaccact cccagagca gcacccaca cctcgtccc 
      ttctagtcc ctcgctact ccaccaacc actcggact 
      tcggccttt cgggccctc gctccctct cggacaact 
      ccagtgctg cggagagcc ccgtccagg acgacatcc 
      tctccaccc aaagcaccc tcggcgtga accacgtca 
      acaacacca acttcccgc tgcaccaga acatgacgt 
      cgtcgtcgt accccagca gcaactgca acaactcaa 
      tctatcccg tccttcctg cgagtcttc tctactcgc 
      agctgtatt cagcggcca accaaagcc acaacttcc 
      actcgctgc actcgcaca cgacgcaaa gccaccaca 
      atgacctgc agacggtca tggaccagc tgtccacca 
      gcaaccagc ggcagatga acggctcca cggacctgc 
      ttctgagca ataatggga cgtgtgcgg cggcggcgg 
      cgagacagg acgatgcca ggttgacga gcaacgggg 
      cccagaggg gccctggac agaacagtg acgcggtgg 
      tgtggaggc cgtattgag tgaccgagg aaacgatgt 
      gatttttgt gcaatattg ttgactagt tttggggtt 
      gttgggact ttgaggggt gtgtttccg ccaagtttc 
      aaacttttc gcatttgaa ctcaacggg aaactatag 
      gtttaggta atctaccag gtttttttt actttgtta 
      cgctcgtta atctaataa aaagtaaca atgttg
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