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Gene information
General information
TC005561 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11320622..11323648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005561
    iBeetle screen iB_03980

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005561 RA
      MPQIPPPKE HKYVDSLWC MYMVSVVGA WNAELVTYP 
      LDLAKTRLQ IQGEVANTK DASMVKAPY RGLFRTAVG 
      IVSEEGFLK LWQGAYAAL YRHLFYSGT RIVTYKHLK 
      DKFFDSGTE QYFPVWKSA LCGVTAGAF AQYIASPAD 
      LLKVQLQME GKRKIMGLP PRVNGLFDA FRKTVETAG 
      VRGLWKGSV PNVQRAALV NLGDLTTYD SAKRFILRN 
      TTLEDNHLV HCLASSMAG LVAALMGTP ADVIKTRVM 
      NQPMDEQGR GLLYKSSID CFKKSVQNE GFGALYKGF 
      LPIWLRMAP WSLTFWLSY EEVLRLLGA EQF
    CDS sequence
    • >TC005561 RA
      atgccgcag attccgccc ccgaaggag cacaagtac 
      gtggactcg ctatggtgc atgtacatg gtatcggtg 
      gtcggagcg tggaatgcg gagttagtg acttacccg 
      ttagatttg gccaaaacg cggctgcaa atacagggt 
      gaggtcgcc aacaccaaa gatgcgagc atggtcaag 
      gccccgtac cgggggctg ttccgcacc gccgtgggg 
      atcgtctcc gaggagggg tttctgaag ctgtggcag 
      ggggcgtac gccgccttg taccgccac ttgttctac 
      tcaggtact cgaattgtc acgtacaag cacctcaaa 
      gacaagttt ttcgactcg ggcaccgag cagtacttc 
      cccgtgtgg aagtcggcc ctttgtggg gtcacagcg 
      ggggccttt gcacagtac attgccagt ccggccgat 
      ttactcaaa gtgcaatta caaatggaa ggcaagcgc 
      aaaatcatg gggctgccg ccgcgagtt aacggcctt 
      ttcgacgcg tttaggaaa acggtcgag actgcgggg 
      gtgaggggg ctgtggaag gggtcagtt ccgaatgtg 
      caaagggcc gctttggtc aatttgggc gacttgacg 
      acctacgat tcggccaaa aggttcatc ttgaggaac 
      actacactt gaagataat catttggtc cactgtctg 
      gcgagtagc atggcgggg ttggtggcg gccctgatg 
      ggaaccccc gcagatgtg attaaaact agagtcatg 
      aaccaacca atggacgaa caggggagg ggtttgttg 
      tataagtcg tccatcgat tgtttcaaa aagtcggta 
      caaaatgag gggtttggg gccctttat aaagggttc 
      ctgcccatt tggttgagg atggcgcct tggtccttg 
      accttctgg ctgtcctat gaggaggta cttcggcta 
      ctaggggcc gagcaattt tga
    mRNA sequence
    • >TC005561 RA
      tcaaattct accaacacc actcacacg tcaccaaca 
      atgtagtcc caaaagcac tgccgtgac aattgttat 
      ctataactg ttcgttatt tacttaact aatgaagtt 
      acggaagtt ccaccaaac aaataaatg gctttttgg 
      tgaagatgc cgcagattc cgcccccga aggagcaca 
      agtacgtgg actcgctat ggtgcatgt acatggtat 
      cggtggtcg gagcgtgga atgcggagt tagtgactt 
      acccgttag atttggcca aaacgcggc tgcaaatac 
      agggtgagg tcgccaaca ccaaagatg cgagcatgg 
      tcaaggccc cgtaccggg ggctgttcc gcaccgccg 
      tggggatcg tctccgagg aggggtttc tgaagctgt 
      ggcaggggg cgtacgccg ccttgtacc gccacttgt 
      tctactcag gtactcgaa ttgtcacgt acaagcacc 
      tcaaagaca agtttttcg actcgggca ccgagcagt 
      acttccccg tgtggaagt cggcccttt gtggggtca 
      cagcggggg cctttgcac agtacattg ccagtccgg 
      ccgatttac tcaaagtgc aattacaaa tggaaggca 
      agcgcaaaa tcatggggc tgccgccgc gagttaacg 
      gccttttcg acgcgttta ggaaaacgg tcgagactg 
      cgggggtga gggggctgt ggaaggggt cagttccga 
      atgtgcaaa gggccgctt tggtcaatt tgggcgact 
      tgacgacct acgattcgg ccaaaaggt tcatcttga 
      ggaacacta cacttgaag ataatcatt tggtccact 
      gtctggcga gtagcatgg cggggttgg tggcggccc 
      tgatgggaa cccccgcag atgtgatta aaactagag 
      tcatgaacc aaccaatgg acgaacagg ggaggggtt 
      tgttgtata agtcgtcca tcgattgtt tcaaaaagt 
      cggtacaaa atgaggggt ttggggccc tttataaag 
      ggttcctgc ccatttggt tgaggatgg cgccttggt 
      ccttgacct tctggctgt cctatgagg aggtacttc 
      ggctactag gggccgagc aattttgat ggtaggcaa 
      ttaggtgca atattattt ttaggcatg gtatcgcat 
      attttttac ttttttttt ataaaagta cctcgcttt 
      aaatagatt tctaactca atggtgcca acttaattt 
      caaattcac aggtcacgt taaaactac aaacgatta 
      taaagcatt aagattaca gtaattgga aactgtcaa 
      ttaataatt aaattacat gatttttca cttgttgtg 
      ttacgaaac tagaattag tcgtgaatt attgagcca 
      gtgccttct taagtcaaa aaatggcag ctgtttttt 
      taattagag ttgtaaagt gtttatttc ccttctcaa 
      tatagcaaa aagcgaacg gaagtgcag ttaaatatt 
      taataactt aatgcaatt gaccgtggc ctgttttta 
      aatattgta ttaaaagat atgtcattt aaagcgcga 
      aggttaatt tattacgag gctgcaata aagtttgga 
      cacatttaa ttaatttta tgaggaaaa ccgagaaaa 
      aataattgt tatttcata tttatcatc tacaaaagc 
      tgagtcatg ttatgcaaa aaacaatca ccgttttaa 
      acatgactt tgtagcgat ttaccacat tttatttaa 
      acgtactta aaaactgat ctcagcagc ttaaaatta 
      ttctttaat ttttaatgt gtatttatt tggctgata 
      tttgaacgg cttaacaag tgttaaaaa cttgttaat 
      atttgattg aaatagtta tgttacgca ctattttgt 
      gcaactgat tgcataagt tgctatcga tcatcggag 
      aattttaac aattaactc ataaaatcc ccgggcgtt 
      gtgaccttt gcggtcgtc ttatcgaga tttcagaaa 
      aattgggac gaatttagc atgaatcac ttgaccaat 
      taccaatga ataacaatt caagaactg ataattgcg 
      ctaaagcag ctcctttgg ggcttcccc aaaaatctc 
      ggacccgtc aatggacca aaatagggt caaggcccc 
      cgccttggc cttcaacta atacgacaa ccttctaag 
      actgttaac tcggagtgg aaaaatatg ggatatcat 
      gctgtgtca agtgggata ttgaaaatt tagttatag 
      aacaaagtg tgttcactg taaaaatca aaacagtcc 
      aaatgtgtc aaattcgca cgaacgtag acgagagcg 
      tagtgccac agttttcca agagcgtac ctatttttt 
      atatttgta caaaaggac aatgtgaac ttgtactct 
      aaagcgcaa tacaaacaa ttttttatt attgtgttt 
      tattttttt aatatttgt cgtgtaaat ttaaccagt 
      ggactaata tttagccta ggacgtaaa ctgttgagc 
      gggaaagga actttccgg tactatttt gttacagat 
      caatgggtg atattaagg aagttgtac attttcatt 
      tgttgtttt aaaattgtg gcttgttga caatatatt 
      gttaaagat aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY