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Gene information
General information
TC034119
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005560
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 4264601..4269808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034119
      iBeetle screen iB_03979
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva dorsalbent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head ventral bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034119 RA
        MDVIFLYFA FFYFYTCQS ASLHKKNET LEILKKDVS 
        QVGSAIENF TFDILGELL DDSEDQETT TIESVRSKR 
        SVEDDVREL CHKRCQKAQ EVETQQIKD LTVEVKKLK 
        QLVKLLQDQ QKIIEVLEQ KNNTGKEAK EEKVEENVP 
        NHEQLAKIK TDLTEVIKD LNETRELID LQKQKDEIL 
        EEELMRQKD EITKLKSIV EKIVQKNET IDDSPHISA 
        RASELTNIR KFLKSLPDD DDDEDDIHA KLIQLEKQI 
        KKKRRQESL FTELKNLEK NLDEDNGDS DLLKQIIKA 
        LKKSEPERK SDSLEITNL SDLEEAINR LRPKESDSA 
        QFQSVLTKL INKPQTPIT ITPQQMSEL TKKLAPNNR 
        PYICIADDG AKNNGYAVP YSRNFPFYP PLDHNQFFP 
        GQVAPPNYY TPGSANGVG SEKQFGYQA NGDKYQEDL 
        KQQIDGLQA AIDSLNRPE YLQRPEDKA IVDDLERQI 
        GDLRSLVTN LNYEGVRAK RSTENNEVE EKNSEELIK 
        EMSHFVNKL FKENNATSQ ARQFGEIQD KINSIKKEL 
        DGGKSRAAN DYAPPSFSE LLNPLPKEV KSSVKNDFF 
        TDVIGRILD KLISAIPIV IQKLFNFSL DELSKNDYS 
        DYDVQVLYK NLGIFAYLP VIILRLVKG ISAFMNMLR 
        KNSFFKNFL MPAIILATV AGAVIFLIW WLQPDTSNN 
        PYGYISDNS DPNYQYAGN YQYSGNNQY PVSTNSPVY 
        SRSYFNK
      CDS sequence
      • >TC034119 RA
        atggacgta attttttta tactttgca tttttttac 
        ttttatacg tgccaaagt gcctcactt cataaaaaa 
        aacgagact cttgaaatt ttgaaaaag gacgtgtca 
        caagtgggg agtgctatt gaaaatttc actttcgat 
        atcctcggt gaattgttg gatgattcc gaggatcaa 
        gaaaccaca acgattgaa agtgtgagg agcaaaagg 
        tcggttgaa gacgatgtc cgagaattg tgccacaaa 
        cggtgccaa aaagcgcaa gaagttgaa acgcaacaa 
        attaaggac ttgacggtt gaggtcaaa aagttgaaa 
        caactcgta aaattgttg caagaccaa caaaaaatt 
        attgaagta cttgagcaa aagaataac acggggaaa 
        gaagcaaaa gaggagaag gttgaagaa aatgtgcca 
        aatcacgaa caactcgcc aaaattaaa actgacttg 
        acggaagtc atcaaggat ttgaacgag acaagggaa 
        ctgatcgac ttacaaaaa caaaaagac gagattttg 
        gaagaagaa ttgatgaga cagaaggat gaaattacc 
        aaacttaaa tcaattgtt gaaaaaata gtacagaaa 
        aacgagaca attgatgat tcccctcac atttcggct 
        cgtgctagt gaattgaca aatatccga aagttccta 
        aagtcactc cctgatgat gacgacgat gaggacgac 
        attcacgcc aaactaatc caactagag aagcaaatt 
        aagaagaag aggcgccag gaaagcctc ttcaccgaa 
        ctgaaaaac ctcgaaaaa aatttagat gaagataac 
        ggggacagc gacctcctg aagcaaatc atcaaagcg 
        ctcaaaaaa tccgaacct gagcgcaaa agcgactcc 
        ctcgaaatc accaattta tcggacttg gaagaggcg 
        ataaaccgt ctgcgcccc aaagagtcc gactcggcc 
        caatttcaa tccgtcttg accaaatta attaataaa 
        ccacaaacg ccaattaca ataaccccc caacagatg 
        agcgagctc actaagaaa ttagcccca aataatcgc 
        ccctacatt tgcatagct gatgacggg gcgaaaaac 
        aacggttat gcagtgccg tattcccgg aatttcccc 
        ttttacccg ccacttgac cacaatcaa ttttttccg 
        ggacaagtt gcgccaccg aattattac actccggga 
        agcgctaat ggggtgggt tctgagaaa cagttcggt 
        tatcaggca aatggtgac aagtaccag gaagacttg 
        aagcaacag atcgacggc cttcaggcg gctattgat 
        agtttgaat cggcctgaa tacttgcaa aggccggaa 
        gataaggcg attgtggat gacttggag cggcaaatc 
        ggcgatttg cggagttta gtcaccaat ttgaattat 
        gaaggggtc agggcgaaa agatcgacc gaaaataac 
        gaagttgaa gaaaaaaac agcgaggaa cttatcaag 
        gagatgagt catttcgtc aataaattg tttaaggag 
        aataacgcg acgagtcaa gcgagacag tttggggaa 
        attcaggac aagattaat tcgatcaaa aaagaatta 
        gatggtggc aaaagccgg gcggctaat gactacgcc 
        ccgccatct ttttccgaa ttattgaac cctttgcca 
        aaagaagtc aagtcaagc gtcaaaaac gacttcttc 
        accgatgtc attggccgt attttggac aaactcatt 
        tcagcaatc cccatagtt atccagaaa ttgttcaac 
        ttcagtctg gacgagttg tccaaaaac gattactcc 
        gattacgac gtccaggtc ctttataaa aatctcggg 
        attttcgcg tatttgccc gtaattatc ttacggctg 
        gtcaaagga atcagtgcc tttatgaac atgttacgc 
        aaaaacagc ttctttaaa aatttccta atgccggcg 
        attatcttg gccacggtg gccggtgcc gtaattttc 
        ctgatttgg tggctgcag cccgacact tcaaataat 
        ccgtacggt tacatcagt gataacagc gacccgaat 
        tatcaatac gccggaaat tatcagtat tcgggaaat 
        aatcaatat ccggtcagc actaatagt ccagtgtat 
        agcaggagc tactttaat aaataa
      mRNA sequence
      • >TC034119 RA
        tgatatcat attgtatcg tttatttat ttcgcgggt 
        ggtcacaag cgaagcgag tgcatcaaa cttttaagc 
        tgaaataca actaacaag ttattgtta cgtaaacct 
        gtttgaatg tttgatttg tctccgtgt tccggcatc 
        gctgcaaga cagcggaca tgaacaata tttatcttt 
        aatcaaatt agctgtccg aataaaaca aactcgata 
        tgcactcca gcaccaaat aaactttat ctaaacacg 
        ggccattaa accttcacg tagaaagcg tacttagtt 
        agaaaaata gcaaaaaag gtaaatgat ctcttatat 
        ctccataaa aaattcact cgttgctta tttcccaca 
        ttacatagg caacttatg gaaattgat gccgccttt 
        ggggaacat aatttttaa gcgttggcc gtgctgtaa 
        ataaccctc gacaaacct acacgaaat gcaaaaacg 
        caattacga aggcttacg atcaattat taaataaaa 
        gacggggtt attaagata aatctcgat cctgtcaga 
        cgcaagcac cacgattca acaaattat tgaattgct 
        aagtgtgtt ccgaggaag cgatttatt aatatacag 
        gaaattaaa tccgtcaac ttgcttaca tttcaaacg 
        attgtttac tggataatg ctaactttg ctagtgata 
        taataaggc tattacgat aatttagag cacaacctt 
        gactgagca acccctatt acaaaactg aattttgag 
        caataacgc gagtaagca ctttgcaaa cgttccaca 
        ttttctgca agtcgggcc agttctaat taaacacaa 
        caccgaaat ggacgtaat ttttttata ctttgcatt 
        tttttactt ttatacgtg ccaaagtgc ctcacttca 
        taaaaaaaa cgagactct tgaaatttt gaaaaagga 
        cgtgtcaca agtggggag tgctattga aaatttcac 
        tttcgatat cctcggtga attgttgga tgattccga 
        ggatcaaga aaccacaac gattgaaag tgtgaggag 
        caaaaggtc ggttgaaga cgatgtccg agaattgtg 
        ccacaaacg gtgccaaaa agcgcaaga agttgaaac 
        gcaacaaat taaggactt gacggttga ggtcaaaaa 
        gttgaaaca actcgtaaa attgttgca agaccaaca 
        aaaaattat tgaagtact tgagcaaaa gaataacac 
        ggggaaaga agcaaaaga ggagaaggt tgaagaaaa 
        tgtgccaaa tcacgaaca actcgccaa aattaaaac 
        tgacttgac ggaagtcat caaggattt gaacgagac 
        aagggaact gatcgactt acaaaaaca aaaagacga 
        gattttgga agaagaatt gatgagaca gaaggatga 
        aattaccaa acttaaatc aattgttga aaaaatagt 
        acagaaaaa cgagacaat tgatgattc ccctcacat 
        ttcggctcg tgctagtga attgacaaa tatccgaaa 
        gttcctaaa gtcactccc tgatgatga cgacgatga 
        ggacgacat tcacgccaa actaatcca actagagaa 
        gcaaattaa gaagaagag gcgccagga aagcctctt 
        caccgaact gaaaaacct cgaaaaaaa tttagatga 
        agataacgg ggacagcga cctcctgaa gcaaatcat 
        caaagcgct caaaaaatc cgaacctga gcgcaaaag 
        cgactccct cgaaatcac caatttatc ggacttgga 
        agaggcgat aaaccgtct gcgccccaa agagtccga 
        ctcggccca atttcaatc cgtcttgac caaattaat 
        taataaacc acaaacgcc aattacaat aacccccca 
        acagatgag cgagctcac taagaaatt agccccaaa 
        taatcgccc ctacatttg catagctga tgacggggc 
        gaaaaacaa cggttatgc agtgccgta ttcccggaa 
        tttcccctt ttacccgcc acttgacca caatcaatt 
        ttttccggg acaagttgc gccaccgaa ttattacac 
        tccgggaag cgctaatgg ggtgggttc tgagaaaca 
        gttcggtta tcaggcaaa tggtgacaa gtaccagga 
        agacttgaa gcaacagat cgacggcct tcaggcggc 
        tattgatag tttgaatcg gcctgaata cttgcaaag 
        gccggaaga taaggcgat tgtggatga cttggagcg 
        gcaaatcgg cgatttgcg gagtttagt caccaattt 
        gaattatga aggggtcag ggcgaaaag atcgaccga 
        aaataacga agttgaaga aaaaaacag cgaggaact 
        tatcaagga gatgagtca tttcgtcaa taaattgtt 
        taaggagaa taacgcgac gagtcaagc gagacagtt 
        tggggaaat tcaggacaa gattaattc gatcaaaaa 
        agaattaga tggtggcaa aagccgggc ggctaatga 
        ctacgcccc gccatcttt ttccgaatt attgaaccc 
        tttgccaaa agaagtcaa gtcaagcgt caaaaacga 
        cttcttcac cgatgtcat tggccgtat tttggacaa 
        actcatttc agcaatccc catagttat ccagaaatt 
        gttcaactt cagtctgga cgagttgtc caaaaacga 
        ttactccga ttacgacgt ccaggtcct ttataaaaa 
        tctcgggat tttcgcgta tttgcccgt aattatctt 
        acggctggt caaaggaat cagtgcctt tatgaacat 
        gttacgcaa aaacagctt ctttaaaaa tttcctaat 
        gccggcgat tatcttggc cacggtggc cggtgccgt 
        aattttcct gatttggtg gctgcagcc cgacacttc 
        aaataatcc gtacggtta catcagtga taacagcga 
        cccgaatta tcaatacgc cggaaatta tcagtattc 
        gggaaataa tcaatatcc ggtcagcac taatagtcc 
        agtgtatag caggagcta ctttaataa ataattttt 
        tactgggaa gtgttttat att