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Gene information
General information
TC034119
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005560 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 4264601..4269808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC034119
      iBeetle screen iB_03979
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva dorsalbent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head ventral bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034119 RA
        MDVIFLYFA FFYFYTCQS ASLHKKNET LEILKKDVS 
QVGSAIENF TFDILGELL DDSEDQETT TIESVRSKR 
SVEDDVREL CHKRCQKAQ EVETQQIKD LTVEVKKLK 
QLVKLLQDQ QKIIEVLEQ KNNTGKEAK EEKVEENVP 
NHEQLAKIK TDLTEVIKD LNETRELID LQKQKDEIL 
EEELMRQKD EITKLKSIV EKIVQKNET IDDSPHISA 
RASELTNIR KFLKSLPDD DDDEDDIHA KLIQLEKQI 
KKKRRQESL FTELKNLEK NLDEDNGDS DLLKQIIKA 
LKKSEPERK SDSLEITNL SDLEEAINR LRPKESDSA 
QFQSVLTKL INKPQTPIT ITPQQMSEL TKKLAPNNR 
PYICIADDG AKNNGYAVP YSRNFPFYP PLDHNQFFP 
GQVAPPNYY TPGSANGVG SEKQFGYQA NGDKYQEDL 
KQQIDGLQA AIDSLNRPE YLQRPEDKA IVDDLERQI 
GDLRSLVTN LNYEGVRAK RSTENNEVE EKNSEELIK 
EMSHFVNKL FKENNATSQ ARQFGEIQD KINSIKKEL 
DGGKSRAAN DYAPPSFSE LLNPLPKEV KSSVKNDFF 
TDVIGRILD KLISAIPIV IQKLFNFSL DELSKNDYS 
DYDVQVLYK NLGIFAYLP VIILRLVKG ISAFMNMLR 
KNSFFKNFL MPAIILATV AGAVIFLIW WLQPDTSNN 
PYGYISDNS DPNYQYAGN YQYSGNNQY PVSTNSPVY 
SRSYFNK
      CDS sequence
      • >TC034119 RA
        atggacgta attttttta tactttgca tttttttac 
ttttatacg tgccaaagt gcctcactt cataaaaaa 
aacgagact cttgaaatt ttgaaaaag gacgtgtca 
caagtgggg agtgctatt gaaaatttc actttcgat 
atcctcggt gaattgttg gatgattcc gaggatcaa 
gaaaccaca acgattgaa agtgtgagg agcaaaagg 
tcggttgaa gacgatgtc cgagaattg tgccacaaa 
cggtgccaa aaagcgcaa gaagttgaa acgcaacaa 
attaaggac ttgacggtt gaggtcaaa aagttgaaa 
caactcgta aaattgttg caagaccaa caaaaaatt 
attgaagta cttgagcaa aagaataac acggggaaa 
gaagcaaaa gaggagaag gttgaagaa aatgtgcca 
aatcacgaa caactcgcc aaaattaaa actgacttg 
acggaagtc atcaaggat ttgaacgag acaagggaa 
ctgatcgac ttacaaaaa caaaaagac gagattttg 
gaagaagaa ttgatgaga cagaaggat gaaattacc 
aaacttaaa tcaattgtt gaaaaaata gtacagaaa 
aacgagaca attgatgat tcccctcac atttcggct 
cgtgctagt gaattgaca aatatccga aagttccta 
aagtcactc cctgatgat gacgacgat gaggacgac 
attcacgcc aaactaatc caactagag aagcaaatt 
aagaagaag aggcgccag gaaagcctc ttcaccgaa 
ctgaaaaac ctcgaaaaa aatttagat gaagataac 
ggggacagc gacctcctg aagcaaatc atcaaagcg 
ctcaaaaaa tccgaacct gagcgcaaa agcgactcc 
ctcgaaatc accaattta tcggacttg gaagaggcg 
ataaaccgt ctgcgcccc aaagagtcc gactcggcc 
caatttcaa tccgtcttg accaaatta attaataaa 
ccacaaacg ccaattaca ataaccccc caacagatg 
agcgagctc actaagaaa ttagcccca aataatcgc 
ccctacatt tgcatagct gatgacggg gcgaaaaac 
aacggttat gcagtgccg tattcccgg aatttcccc 
ttttacccg ccacttgac cacaatcaa ttttttccg 
ggacaagtt gcgccaccg aattattac actccggga 
agcgctaat ggggtgggt tctgagaaa cagttcggt 
tatcaggca aatggtgac aagtaccag gaagacttg 
aagcaacag atcgacggc cttcaggcg gctattgat 
agtttgaat cggcctgaa tacttgcaa aggccggaa 
gataaggcg attgtggat gacttggag cggcaaatc 
ggcgatttg cggagttta gtcaccaat ttgaattat 
gaaggggtc agggcgaaa agatcgacc gaaaataac 
gaagttgaa gaaaaaaac agcgaggaa cttatcaag 
gagatgagt catttcgtc aataaattg tttaaggag 
aataacgcg acgagtcaa gcgagacag tttggggaa 
attcaggac aagattaat tcgatcaaa aaagaatta 
gatggtggc aaaagccgg gcggctaat gactacgcc 
ccgccatct ttttccgaa ttattgaac cctttgcca 
aaagaagtc aagtcaagc gtcaaaaac gacttcttc 
accgatgtc attggccgt attttggac aaactcatt 
tcagcaatc cccatagtt atccagaaa ttgttcaac 
ttcagtctg gacgagttg tccaaaaac gattactcc 
gattacgac gtccaggtc ctttataaa aatctcggg 
attttcgcg tatttgccc gtaattatc ttacggctg 
gtcaaagga atcagtgcc tttatgaac atgttacgc 
aaaaacagc ttctttaaa aatttccta atgccggcg 
attatcttg gccacggtg gccggtgcc gtaattttc 
ctgatttgg tggctgcag cccgacact tcaaataat 
ccgtacggt tacatcagt gataacagc gacccgaat 
tatcaatac gccggaaat tatcagtat tcgggaaat 
aatcaatat ccggtcagc actaatagt ccagtgtat 
agcaggagc tactttaat aaataa
      mRNA sequence
      • >TC034119 RA
        tgatatcat attgtatcg tttatttat ttcgcgggt 
ggtcacaag cgaagcgag tgcatcaaa cttttaagc 
tgaaataca actaacaag ttattgtta cgtaaacct 
gtttgaatg tttgatttg tctccgtgt tccggcatc 
gctgcaaga cagcggaca tgaacaata tttatcttt 
aatcaaatt agctgtccg aataaaaca aactcgata 
tgcactcca gcaccaaat aaactttat ctaaacacg 
ggccattaa accttcacg tagaaagcg tacttagtt 
agaaaaata gcaaaaaag gtaaatgat ctcttatat 
ctccataaa aaattcact cgttgctta tttcccaca 
ttacatagg caacttatg gaaattgat gccgccttt 
ggggaacat aatttttaa gcgttggcc gtgctgtaa 
ataaccctc gacaaacct acacgaaat gcaaaaacg 
caattacga aggcttacg atcaattat taaataaaa 
gacggggtt attaagata aatctcgat cctgtcaga 
cgcaagcac cacgattca acaaattat tgaattgct 
aagtgtgtt ccgaggaag cgatttatt aatatacag 
gaaattaaa tccgtcaac ttgcttaca tttcaaacg 
attgtttac tggataatg ctaactttg ctagtgata 
taataaggc tattacgat aatttagag cacaacctt 
gactgagca acccctatt acaaaactg aattttgag 
caataacgc gagtaagca ctttgcaaa cgttccaca 
ttttctgca agtcgggcc agttctaat taaacacaa 
caccgaaat ggacgtaat ttttttata ctttgcatt 
tttttactt ttatacgtg ccaaagtgc ctcacttca 
taaaaaaaa cgagactct tgaaatttt gaaaaagga 
cgtgtcaca agtggggag tgctattga aaatttcac 
tttcgatat cctcggtga attgttgga tgattccga 
ggatcaaga aaccacaac gattgaaag tgtgaggag 
caaaaggtc ggttgaaga cgatgtccg agaattgtg 
ccacaaacg gtgccaaaa agcgcaaga agttgaaac 
gcaacaaat taaggactt gacggttga ggtcaaaaa 
gttgaaaca actcgtaaa attgttgca agaccaaca 
aaaaattat tgaagtact tgagcaaaa gaataacac 
ggggaaaga agcaaaaga ggagaaggt tgaagaaaa 
tgtgccaaa tcacgaaca actcgccaa aattaaaac 
tgacttgac ggaagtcat caaggattt gaacgagac 
aagggaact gatcgactt acaaaaaca aaaagacga 
gattttgga agaagaatt gatgagaca gaaggatga 
aattaccaa acttaaatc aattgttga aaaaatagt 
acagaaaaa cgagacaat tgatgattc ccctcacat 
ttcggctcg tgctagtga attgacaaa tatccgaaa 
gttcctaaa gtcactccc tgatgatga cgacgatga 
ggacgacat tcacgccaa actaatcca actagagaa 
gcaaattaa gaagaagag gcgccagga aagcctctt 
caccgaact gaaaaacct cgaaaaaaa tttagatga 
agataacgg ggacagcga cctcctgaa gcaaatcat 
caaagcgct caaaaaatc cgaacctga gcgcaaaag 
cgactccct cgaaatcac caatttatc ggacttgga 
agaggcgat aaaccgtct gcgccccaa agagtccga 
ctcggccca atttcaatc cgtcttgac caaattaat 
taataaacc acaaacgcc aattacaat aacccccca 
acagatgag cgagctcac taagaaatt agccccaaa 
taatcgccc ctacatttg catagctga tgacggggc 
gaaaaacaa cggttatgc agtgccgta ttcccggaa 
tttcccctt ttacccgcc acttgacca caatcaatt 
ttttccggg acaagttgc gccaccgaa ttattacac 
tccgggaag cgctaatgg ggtgggttc tgagaaaca 
gttcggtta tcaggcaaa tggtgacaa gtaccagga 
agacttgaa gcaacagat cgacggcct tcaggcggc 
tattgatag tttgaatcg gcctgaata cttgcaaag 
gccggaaga taaggcgat tgtggatga cttggagcg 
gcaaatcgg cgatttgcg gagtttagt caccaattt 
gaattatga aggggtcag ggcgaaaag atcgaccga 
aaataacga agttgaaga aaaaaacag cgaggaact 
tatcaagga gatgagtca tttcgtcaa taaattgtt 
taaggagaa taacgcgac gagtcaagc gagacagtt 
tggggaaat tcaggacaa gattaattc gatcaaaaa 
agaattaga tggtggcaa aagccgggc ggctaatga 
ctacgcccc gccatcttt ttccgaatt attgaaccc 
tttgccaaa agaagtcaa gtcaagcgt caaaaacga 
cttcttcac cgatgtcat tggccgtat tttggacaa 
actcatttc agcaatccc catagttat ccagaaatt 
gttcaactt cagtctgga cgagttgtc caaaaacga 
ttactccga ttacgacgt ccaggtcct ttataaaaa 
tctcgggat tttcgcgta tttgcccgt aattatctt 
acggctggt caaaggaat cagtgcctt tatgaacat 
gttacgcaa aaacagctt ctttaaaaa tttcctaat 
gccggcgat tatcttggc cacggtggc cggtgccgt 
aattttcct gatttggtg gctgcagcc cgacacttc 
aaataatcc gtacggtta catcagtga taacagcga 
cccgaatta tcaatacgc cggaaatta tcagtattc 
gggaaataa tcaatatcc ggtcagcac taatagtcc 
agtgtatag caggagcta ctttaataa ataattttt 
tactgggaa gtgttttat att