General information | |||
TC034119
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 11327831..11333038 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC034119 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03979 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
larva dorsalbent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head ventral bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC034119 RA
MDVIFLYFA FFYFYTCQS ASLHKKNET LEILKKDVS QVGSAIENF TFDILGELL DDSEDQETT TIESVRSKR SVEDDVREL CHKRCQKAQ EVETQQIKD LTVEVKKLK QLVKLLQDQ QKIIEVLEQ KNNTGKEAK EEKVEENVP NHEQLAKIK TDLTEVIKD LNETRELID LQKQKDEIL EEELMRQKD EITKLKSIV EKIVQKNET IDDSPHISA RASELTNIR KFLKSLPDD DDDEDDIHA KLIQLEKQI KKKRRQESL FTELKNLEK NLDEDNGDS DLLKQIIKA LKKSEPERK SDSLEITNL SDLEEAINR LRPKESDSA QFQSVLTKL INKPQTPIT ITPQQMSEL TKKLAPNNR PYICIADDG AKNNGYAVP YSRNFPFYP PLDHNQFFP GQVAPPNYY TPGSANGVG SEKQFGYQA NGDKYQEDL KQQIDGLQA AIDSLNRPE YLQRPEDKA IVDDLERQI GDLRSLVTN LNYEGVRAK RSTENNEVE EKNSEELIK EMSHFVNKL FKENNATSQ ARQFGEIQD KINSIKKEL DGGKSRAAN DYAPPSFSE LLNPLPKEV KSSVKNDFF TDVIGRILD KLISAIPIV IQKLFNFSL DELSKNDYS DYDVQVLYK NLGIFAYLP VIILRLVKG ISAFMNMLR KNSFFKNFL MPAIILATV AGAVIFLIW WLQPDTSNN PYGYISDNS DPNYQYAGN YQYSGNNQY PVSTNSPVY SRSYFNK
- >TC034119 RA
atggacgta attttttta tactttgca tttttttac ttttatacg tgccaaagt gcctcactt cataaaaaa aacgagact cttgaaatt ttgaaaaag gacgtgtca caagtgggg agtgctatt gaaaatttc actttcgat atcctcggt gaattgttg gatgattcc gaggatcaa gaaaccaca acgattgaa agtgtgagg agcaaaagg tcggttgaa gacgatgtc cgagaattg tgccacaaa cggtgccaa aaagcgcaa gaagttgaa acgcaacaa attaaggac ttgacggtt gaggtcaaa aagttgaaa caactcgta aaattgttg caagaccaa caaaaaatt attgaagta cttgagcaa aagaataac acggggaaa gaagcaaaa gaggagaag gttgaagaa aatgtgcca aatcacgaa caactcgcc aaaattaaa actgacttg acggaagtc atcaaggat ttgaacgag acaagggaa ctgatcgac ttacaaaaa caaaaagac gagattttg gaagaagaa ttgatgaga cagaaggat gaaattacc aaacttaaa tcaattgtt gaaaaaata gtacagaaa aacgagaca attgatgat tcccctcac atttcggct cgtgctagt gaattgaca aatatccga aagttccta aagtcactc cctgatgat gacgacgat gaggacgac attcacgcc aaactaatc caactagag aagcaaatt aagaagaag aggcgccag gaaagcctc ttcaccgaa ctgaaaaac ctcgaaaaa aatttagat gaagataac ggggacagc gacctcctg aagcaaatc atcaaagcg ctcaaaaaa tccgaacct gagcgcaaa agcgactcc ctcgaaatc accaattta tcggacttg gaagaggcg ataaaccgt ctgcgcccc aaagagtcc gactcggcc caatttcaa tccgtcttg accaaatta attaataaa ccacaaacg ccaattaca ataaccccc caacagatg agcgagctc actaagaaa ttagcccca aataatcgc ccctacatt tgcatagct gatgacggg gcgaaaaac aacggttat gcagtgccg tattcccgg aatttcccc ttttacccg ccacttgac cacaatcaa ttttttccg ggacaagtt gcgccaccg aattattac actccggga agcgctaat ggggtgggt tctgagaaa cagttcggt tatcaggca aatggtgac aagtaccag gaagacttg aagcaacag atcgacggc cttcaggcg gctattgat agtttgaat cggcctgaa tacttgcaa aggccggaa gataaggcg attgtggat gacttggag cggcaaatc ggcgatttg cggagttta gtcaccaat ttgaattat gaaggggtc agggcgaaa agatcgacc gaaaataac gaagttgaa gaaaaaaac agcgaggaa cttatcaag gagatgagt catttcgtc aataaattg tttaaggag aataacgcg acgagtcaa gcgagacag tttggggaa attcaggac aagattaat tcgatcaaa aaagaatta gatggtggc aaaagccgg gcggctaat gactacgcc ccgccatct ttttccgaa ttattgaac cctttgcca aaagaagtc aagtcaagc gtcaaaaac gacttcttc accgatgtc attggccgt attttggac aaactcatt tcagcaatc cccatagtt atccagaaa ttgttcaac ttcagtctg gacgagttg tccaaaaac gattactcc gattacgac gtccaggtc ctttataaa aatctcggg attttcgcg tatttgccc gtaattatc ttacggctg gtcaaagga atcagtgcc tttatgaac atgttacgc aaaaacagc ttctttaaa aatttccta atgccggcg attatcttg gccacggtg gccggtgcc gtaattttc ctgatttgg tggctgcag cccgacact tcaaataat ccgtacggt tacatcagt gataacagc gacccgaat tatcaatac gccggaaat tatcagtat tcgggaaat aatcaatat ccggtcagc actaatagt ccagtgtat agcaggagc tactttaat aaataa
- >TC034119 RA
tgatatcat attgtatcg tttatttat ttcgcgggt ggtcacaag cgaagcgag tgcatcaaa cttttaagc tgaaataca actaacaag ttattgtta cgtaaacct gtttgaatg tttgatttg tctccgtgt tccggcatc gctgcaaga cagcggaca tgaacaata tttatcttt aatcaaatt agctgtccg aataaaaca aactcgata tgcactcca gcaccaaat aaactttat ctaaacacg ggccattaa accttcacg tagaaagcg tacttagtt agaaaaata gcaaaaaag gtaaatgat ctcttatat ctccataaa aaattcact cgttgctta tttcccaca ttacatagg caacttatg gaaattgat gccgccttt ggggaacat aatttttaa gcgttggcc gtgctgtaa ataaccctc gacaaacct acacgaaat gcaaaaacg caattacga aggcttacg atcaattat taaataaaa gacggggtt attaagata aatctcgat cctgtcaga cgcaagcac cacgattca acaaattat tgaattgct aagtgtgtt ccgaggaag cgatttatt aatatacag gaaattaaa tccgtcaac ttgcttaca tttcaaacg attgtttac tggataatg ctaactttg ctagtgata taataaggc tattacgat aatttagag cacaacctt gactgagca acccctatt acaaaactg aattttgag caataacgc gagtaagca ctttgcaaa cgttccaca ttttctgca agtcgggcc agttctaat taaacacaa caccgaaat ggacgtaat ttttttata ctttgcatt tttttactt ttatacgtg ccaaagtgc ctcacttca taaaaaaaa cgagactct tgaaatttt gaaaaagga cgtgtcaca agtggggag tgctattga aaatttcac tttcgatat cctcggtga attgttgga tgattccga ggatcaaga aaccacaac gattgaaag tgtgaggag caaaaggtc ggttgaaga cgatgtccg agaattgtg ccacaaacg gtgccaaaa agcgcaaga agttgaaac gcaacaaat taaggactt gacggttga ggtcaaaaa gttgaaaca actcgtaaa attgttgca agaccaaca aaaaattat tgaagtact tgagcaaaa gaataacac ggggaaaga agcaaaaga ggagaaggt tgaagaaaa tgtgccaaa tcacgaaca actcgccaa aattaaaac tgacttgac ggaagtcat caaggattt gaacgagac aagggaact gatcgactt acaaaaaca aaaagacga gattttgga agaagaatt gatgagaca gaaggatga aattaccaa acttaaatc aattgttga aaaaatagt acagaaaaa cgagacaat tgatgattc ccctcacat ttcggctcg tgctagtga attgacaaa tatccgaaa gttcctaaa gtcactccc tgatgatga cgacgatga ggacgacat tcacgccaa actaatcca actagagaa gcaaattaa gaagaagag gcgccagga aagcctctt caccgaact gaaaaacct cgaaaaaaa tttagatga agataacgg ggacagcga cctcctgaa gcaaatcat caaagcgct caaaaaatc cgaacctga gcgcaaaag cgactccct cgaaatcac caatttatc ggacttgga agaggcgat aaaccgtct gcgccccaa agagtccga ctcggccca atttcaatc cgtcttgac caaattaat taataaacc acaaacgcc aattacaat aacccccca acagatgag cgagctcac taagaaatt agccccaaa taatcgccc ctacatttg catagctga tgacggggc gaaaaacaa cggttatgc agtgccgta ttcccggaa tttcccctt ttacccgcc acttgacca caatcaatt ttttccggg acaagttgc gccaccgaa ttattacac tccgggaag cgctaatgg ggtgggttc tgagaaaca gttcggtta tcaggcaaa tggtgacaa gtaccagga agacttgaa gcaacagat cgacggcct tcaggcggc tattgatag tttgaatcg gcctgaata cttgcaaag gccggaaga taaggcgat tgtggatga cttggagcg gcaaatcgg cgatttgcg gagtttagt caccaattt gaattatga aggggtcag ggcgaaaag atcgaccga aaataacga agttgaaga aaaaaacag cgaggaact tatcaagga gatgagtca tttcgtcaa taaattgtt taaggagaa taacgcgac gagtcaagc gagacagtt tggggaaat tcaggacaa gattaattc gatcaaaaa agaattaga tggtggcaa aagccgggc ggctaatga ctacgcccc gccatcttt ttccgaatt attgaaccc tttgccaaa agaagtcaa gtcaagcgt caaaaacga cttcttcac cgatgtcat tggccgtat tttggacaa actcatttc agcaatccc catagttat ccagaaatt gttcaactt cagtctgga cgagttgtc caaaaacga ttactccga ttacgacgt ccaggtcct ttataaaaa tctcgggat tttcgcgta tttgcccgt aattatctt acggctggt caaaggaat cagtgcctt tatgaacat gttacgcaa aaacagctt ctttaaaaa tttcctaat gccggcgat tatcttggc cacggtggc cggtgccgt aattttcct gatttggtg gctgcagcc cgacacttc aaataatcc gtacggtta catcagtga taacagcga cccgaatta tcaatacgc cggaaatta tcagtattc gggaaataa tcaatatcc ggtcagcac taatagtcc agtgtatag caggagcta ctttaataa ataattttt tactgggaa gtgttttat att
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
