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Gene information
General information
TC034846 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11775862..11783076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034846
    iBeetle screen iB_03973

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034846 RA
      MNATSGTLI NCAWWQRQG LEVGEPRKL RCDEFLETG 
      HRPPSEYPW YEDAGSSPS LRSPKFMES LPNVSYKGW 
      WTYPYYSCK SRRWLISYS VPIPPPGRG GLKGFLSLD 
      VDVSHLEVN QCEPTTPIP TNSNEISIF HGTHKCHNS 
      TSQCLYQAQ TASSNWSRG KYLCVCRPG YYSPHYDGV 
      FNGTLVEVS WQNREENTS EGDSWSTIF QCRKCAPGC 
      HSCTDPSPC LATYHWPFR ITLLVFSVS CALCTMIIN 
      IYMFKHRKL KVFKVASPI FLSITLFGC AIMYMEMAA 
      IFPVLDAYS CIATKWSRH LGFCITYTA LLMKTWRVS 
      LTYRVKSAH KVKLTDKQL LQWMVPILL VMLIYLGTW 
      TLSDTPTAE EIVDNEDLR FKQCVYNWW DHSLAIGEV 
      LFLAWGIRV CYNVRNAQS LFNEARLIS FAIYNIAIV 
      NILMIAFHL FIFPRAGPD IKYLLGFIR TQLSTSVTV 
      ALVFGPKII RVLRGLGDQ WDNRARSRG VTASFSLNG 
      IGLVPEEAP DLYQENEEL KEEIQKLAA QIEFMKIVH 
      MEINNRHVK PKSGGYFTT LNAPTVVHS PLSRQGCLL 
      SKAPEEL
    CDS sequence
    • >TC034846 RA
      atgaacgcg acttctggc actttgata aactgtgct 
      tggtggcag cgccaaggc ctggaggtt ggagagccg 
      cgaaaattg cgctgcgac gaattcctg gaaacgggt 
      caccgacca ccttcggaa tacccatgg tatgaagat 
      gccggctct agtccgagt ttgcgttcg cccaagttc 
      atggaaagc ttaccgaac gtgtcgtac aaggggtgg 
      tggacctac ccgtactac tcgtgcaag tccaggcgg 
      tggctcatc tcgtacagc gtccccatc ccgccccca 
      ggacgtgga ggtctgaaa ggtttcttg agtttggac 
      gtggatgtg tcgcacctg gaagtcaat cagtgcgag 
      ccaaccaca cccattccg actaattcc aatgaaata 
      tcaatcttc catggcaca cacaaatgt cacaacagc 
      acgtctcag tgtctatat caagcgcaa acagcatcc 
      tctaattgg tccagaggg aagtatctc tgcgtgtgc 
      cgaccgggg tattattcg ccgcattac gatggcgtt 
      ttcaacggg acgctagtc gaggtttcg tggcagaat 
      cgcgaggag aacaccagc gagggcgac tcctggagc 
      acgattttc cagtgtcgg aaatgtgcc cccggttgc 
      cactcctgc actgacccc tcgccttgt ctcgccaca 
      tatcactgg cccttcagg atcactttg ctggttttc 
      agtgttagt tgtgccctt tgtacgatg attatcaac 
      atctacatg ttcaaacac aggaaactg aaagttttc 
      aaagttgca agtcctatc tttctcagt attacactc 
      ttcggttgt gccattatg tacatggaa atggctgcg 
      atttttcca gttttggac gcgtattct tgtattgcg 
      accaagtgg tcgcgacat ttgggtttc tgtatcact 
      tatactgcg ctattgatg aagacgtgg agagtttcg 
      ttaacttat agggtcaag tcggcacat aaagtcaaa 
      ttgactgat aagcaactt ttacaatgg atggttccg 
      attttgtta gttatgttg atttatttg gggacgtgg 
      acgctttcg gacactcca acggcggag gaaatcgtc 
      gataatgag gatttgagg ttcaaacag tgcgtgtac 
      aactggtgg gatcacagc ttggctatc ggcgaggtc 
      ctgttcctg gcatggggc atccgcgtg tgctacaac 
      gtccgcaac gcccagtcc ctcttcaac gaagcgcga 
      ctaatcagt ttcgcaatc tacaacatt gcaatcgta 
      aacatcctc atgatcgcc ttccacttg tttatcttc 
      cctcgagcc ggccctgac ataaaatat ctgctgggg 
      ttcatccga acccaatta tccacaagc gtgaccgtg 
      gccttggtc ttcggcccc aaaataatc cgcgtcttg 
      cgcggcttg ggggaccag tgggacaac agggccagg 
      tcccggggc gtcacagcc tccttctcc ctcaacggg 
      atcgggctg gtgccggag gaagcgccc gatttgtac 
      caagaaaac gaggaattg aaagaggag atccagaaa 
      ttagccgcg cagatcgag ttcatgaaa atcgtacac 
      atggagatc aacaacagg cacgtcaag cccaaaagt 
      gggggctat tttacgact ttgaacgcc ccgacggtg 
      gtccacagc cccctgtcg aggcagggc tgcctgctc 
      agcaaagcc cccgaggag ctgtga
    mRNA sequence
    • >TC034846 RA
      cacgtgttt taacaatga acgcgactt ctggcactt 
      tgataaact gtgcttggt ggcagcgcc aaggcctgg 
      aggttggag agccgcgaa aattgcgct gcgacgaat 
      tcctggaaa cgggtcacc gaccacctt cggaatacc 
      catggtatg aagatgccg gctctagtc cgagtttgc 
      gttcgccca agttcatgg aaagcttac cgaacgtgt 
      cgtacaagg ggtggtgga cctacccgt actactcgt 
      gcaagtcca ggcggtggc tcatctcgt acagcgtcc 
      ccatcccgc ccccaggac gtggaggtc tgaaaggtt 
      tcttgagtt tggacgtgg atgtgtcgc acctggaag 
      tcaatcagt gcgagccaa ccacaccca ttccgacta 
      attccaatg aaatatcaa tcttccatg gcacacaca 
      aatgtcaca acagcacgt ctcagtgtc tatatcaag 
      cgcaaacag catcctcta attggtcca gagggaagt 
      atctctgcg tgtgccgac cggggtatt attcgccgc 
      attacgatg gcgttttca acgggacgc tagtcgagg 
      tttcgtggc agaatcgcg aggagaaca ccagcgagg 
      gcgactcct ggagcacga ttttccagt gtcggaaat 
      gtgcccccg gttgccact cctgcactg acccctcgc 
      cttgtctcg ccacatatc actggccct tcaggatca 
      ctttgctgg ttttcagtg ttagttgtg ccctttgta 
      cgatgatta tcaacatct acatgttca aacacagga 
      aactgaaag ttttcaaag ttgcaagtc ctatctttc 
      tcagtatta cactcttcg gttgtgcca ttatgtaca 
      tggaaatgg ctgcgattt ttccagttt tggacgcgt 
      attcttgta ttgcgacca agtggtcgc gacatttgg 
      gtttctgta tcacttata ctgcgctat tgatgaaga 
      cgtggagag tttcgttaa cttataggg tcaagtcgg 
      cacataaag tcaaattga ctgataagc aacttttac 
      aatggatgg ttccgattt tgttagtta tgttgattt 
      atttgggga cgtggacgc tttcggaca ctccaacgg 
      cggaggaaa tcgtcgata atgaggatt tgaggttca 
      aacagtgcg tgtacaact ggtgggatc acagcttgg 
      ctatcggcg aggtcctgt tcctggcat ggggcatcc 
      gcgtgtgct acaacgtcc gcaacgccc agtccctct 
      tcaacgaag cgcgactaa tcagtttcg caatctaca 
      acattgcaa tcgtaaaca tcctcatga tcgccttcc 
      acttgttta tcttccctc gagccggcc ctgacataa 
      aatatctgc tggggttca tccgaaccc aattatcca 
      caagcgtga ccgtggcct tggtcttcg gccccaaaa 
      taatccgcg tcttgcgcg gcttggggg accagtggg 
      acaacaggg ccaggtccc ggggcgtca cagcctcct 
      tctccctca acgggatcg ggctggtgc cggaggaag 
      cgcccgatt tgtaccaag aaaacgagg aattgaaag 
      aggagatcc agaaattag ccgcgcaga tcgagttca 
      tgaaaatcg tacacatgg agatcaaca acaggcacg 
      tcaagccca aaagtgggg gctatttta cgactttga 
      acgccccga cggtggtcc acagccccc tgtcgaggc 
      agggctgcc tgctcagca aagcccccg aggagctgt 
      gacccccag atcgcaaaa agccggttt ttgtttaat 
      ttccaggaa gtagtcttc catcattcg gttcccttc 
      ggctcaaat ccaaacgat tcgtttact tattcaaaa 
      tcaaaaagc taaacgact gatttttat aatagattg 
      taggatcgc ttaccttgt gtgatattg tagggaaga 
      gttatagac gcggtagga gttactaga gcgacatat 
      ttattaaag acaaactgt ttgatgtaa ttaatgttg 
      atctatgtc aaaaattta agcatcaat gttgaacag 
      gtgttcgtt tttgttggt tgatttgta aatgtgtag 
      gttgttcaa taaatcagg aaataccgc agttctctt 
      ccagaacaa ccgttccga agcacagtg gcggtgttt 
      cccctcaac tgagcggta caggaataa aatgcattt 
      tcgttgttc gttgattta gtcggagaa attcggttc 
      gcaaaatta attcgcttt ttactcctc tacccttca 
      cttttgtga tagataacg tagtcggtg aacgacaat 
      acaatatat caattactc aagacagtc agtacctgc 
      ttgtttatc aaaaaattg gccgacaac tccaactta 
      aatattctc aatgtttgt tgtactcga tgttgacaa 
      ttgatgttt tagtgtcgc agtctgtgg cacaaaaat 
      gctaaatga gccctcaca cgagaaatt tataatgca 
      ttttataaa tttcgcaat ttttttgca ccgcagact 
      gagctgtag cactcgata tttaaactg gaactgttg 
      gcggaatct tggtactgt cgctactta ttaagttgg 
      ttgttaatt tattccaaa tgttctata tatttgtat 
      tacaataaa tgtgtttgt aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY