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Gene information
General information
TC034132
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005513,TC005514
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11865227..11882753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034132
    iBeetle screen iB_03972

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034132 RA
      MADADSKDR YDPELKYLC VDRNNFNDP ATQAEWTQK 
      RLVWVPHEA QGFVAASIK GERGDEVEV ELQETGKRT 
      TVARDDIQK MNPPKFDKV EDMAELTCL NEACVLHNL 
      KDRYYSGLI YTYSGLFCV VVNPYKKLP IYTEKIMER 
      YKGIKRHEV PPHVFAITD TAYRSMLQE REDQSILCT 
      GESGAGKTE NTKKVIQYL AYVAASKSP KGSGAQKDL 
      IGGLEQQLL QANPILEAF GNAKTIKND NSSRFGKFI 
      RINFDASGY IAGANIETY LLEKSRAIR QAKQERTFH 
      IFYQLLAGA SAEQKKEFI LEDPKSYPF LREDNHIVP 
      GVDDSAEFQ ATVKSMNIM GMTNEDFSA IFRVVSAVM 
      LFGTMQFKQ DRNSDQATL PDNTVAQKI AHLLGLSVT 
      DMTKAFLKP RIKVGRDFV TKSQTKEQV EFAVEAISK 
      ACYERMFRW LVTRINRSL GRTKRQGAS FIGILDIAG 
      FEIFELNSF EQLCINYTN EKLQQLFNH TMFILEQEE 
      YQREGIEWK FIDFGLDLQ PTIDLIDKP MGIMALLDE 
      ECLFPKATD KTFVDKLVS AHSVHPKFK KSDFRGVAD 
      FSIIHYAGK VDYCANQWL MKNMDPQNE NVVSLLQAS 
      QDPFVVHIW KDAESIGRA KGMFRTVSY LYKEQLANL 
      MVTLRNTNP NFVRCIIPN HEKRAGKID APLVLDQLR 
      CNGVLEGIR ICRQGFPNR IPFQEFRQR YELLTPNVI 
      NKGFMDGKK ACETMIKSL ELDQNLYRI GQSKIFFRA 
      GVLAHLEEE RDYKITDLI VNFQAFCRG FLSRRNYQK 
      RVQQLNAIR IIQRNCSAY LKLRNWQWW RLYTKVKPL 
      LEVTKQEEK LMQKEDELK QVKDKLEHH IKAAKEFET 
      KYQQAQEEK VMLQEQLQA EVELCAEAE EMRARLTAR 
      KQELEEILH DLEARIEEE EERANTLSN DKKKLQLTI 
      QDLEEQLEE EEGARQKLQ LEKVQCDAK IKKLEEDLA 
      LSEDTNQKL LKEKKVLEE RSNDLSQAL AEEEEKAKH 
      LAKLKAKHE ATIAELEER LLKDHQQRQ ESDRTKRKV 
      ETEVNDLKE QLAEKKSQL EELQLQLGK REEELAQAM 
      VRVDEEGAL KAQAQKALR EMESQLSEL QEDLEAEKA 
      ARSKAEKLK RDLNEELEA LKNELLDSL DTTAAQQEL 
      RSKREQELA NLKKNLEEE NQVHEATLA DMRHKHTQE 
      LASLNEQLE NIKKQKASI EKAKQGLEA ENADLTSEL 
      RNVGASRQE GERRRKQAE SQLVEIQGK LGEIERARA 
      ELAEKNQKL QQEMDNVVQ QLEEAELKA SAAVKNQGT 
      IESQFAEAQ SALEEETRQ KLALSSKLR QLESDKENL 
      QEQIEEEEE AKKNLEKQL NAVMVQLAE AKKKAEDEA 
      EQSAILEES KKKLAKDLE LLQRQVEEL TAANDKLER 
      SKKKIAAEL EDTNIDLEA QRQKVAELE KKQKNFDKV 
      LAEEKQVSE QLMAEKDAA EREAREKET RVLSLSREL 
      DESNVKVEE LERIKRQLQ AELDELVNN QGTADKNVH 
      ELEKAKRSL ETQLAELKA QNEELEDEL QLTEDAKLR 
      LEVNMQALR AQFERDVQA KEEQAEEKR RGIVKQLRD 
      LEAELDEER KQRAAAVTA RKKLEGDLK ELEAQIEMQ 
      CKMKEDALK QLKKSQQQC KEAVRDAEE ARASRDELA 
      AGCKEAERK VKALEAEVL QLSEDHASS ERARRAAEA 
      ERDELLEEV NNTNSKGAL LIDEKRRLE ARIATLEEE 
      IEEEQSNNE LLQDRARKA QLTIEQLTS ELATERSLA 
      QKQESGKLL LERQNKELK AKLSELETA QRTKTKATI 
      AALESKIGN LEEQLEVEA KERLAQQKT NRKLDKKLK 
      ELVMQLEDE RRHADQYKE QVDKANARV KTLKRQLDE 
      AEEEITREK AQKRKVQRD YEDMLESNE SMTRELNSL 
      KTKLRRGTG ASSGMGLPR LSGRGSIQS GGNGSGDDS 
      TTQDDAIDG EDAPN
    CDS sequence
    • >TC034132 RA
      atggcggac gccgacagc aaggaccgc tacgatccc 
      gaactgaag tacctctgc gtggaccgg aacaacttc 
      aacgacccg gccacccag gccgagtgg acccagaag 
      cggctggta tgggtgccg cacgaggcg cagggcttc 
      gtcgccgcc agcatcaag ggcgagcgc ggcgacgag 
      gtcgaggtg gagctccag gagacgggg aagcgcacc 
      accgtcgcc agagacgac atccagaag atgaacccg 
      cccaagttc gacaaggtc gaggacatg gcggaactc 
      acctgcctc aacgaggcc tgtgtcttg cacaacttg 
      aaagacaga tactattcg ggactgatt tatacttac 
      tccggccta ttttgcgtc gttgtcaac ccctacaaa 
      aagctccct atttacaca gaaaaaatt atggaacgc 
      tacaagggc attaagcga cacgaagtg cctcctcat 
      gtctttgcc ataaccgac acggcctat cgttcgatg 
      ttacaagag cgagaagac cagtctata ctttgtacg 
      ggcgagtca ggtgccggt aaaacagaa aacacgaaa 
      aaagtcatt cagtatttg gcctatgtt gccgcctcg 
      aagtcacca aaaggctca ggagcgcag aaagacctt 
      attggcggt ttggagcaa caactgtta caagccaat 
      ccgatcctt gaagctttt ggtaacgca aaaacaatc 
      aaaaacgac aattcttcc agatttgga aaattcatt 
      cggataaat ttcgacgct tccggttac atagccggt 
      gcaaacatc gagacttac cttcttgaa aaatcacgt 
      gccatacga caagccaag caggaacga accttccat 
      atcttctac caactattg gctggagct tccgccgag 
      cagaaaaag gagtttatt ctcgaggac ccaaaatcg 
      tacccattc cttcgtgaa gacaatcac atagtgccc 
      ggagtggac gattcggcc gagttccag gccaccgtc 
      aagagtatg aatataatg gggatgacg aacgaagat 
      ttcagtgcc attttccgc gttgtttcg gccgtaatg 
      ctcttcgga acgatgcaa ttcaaacag gatcgtaat 
      tcggatcag gcgacgtta cccgacaac acggtggcg 
      caaaaaatc gcccatttg ctgggtctg tctgtaact 
      gacatgacc aaggctttc ctcaaaccc aggattaag 
      gtcgggagg gatttcgtc acgaagagc caaacgaaa 
      gaacaggtg gaattcgct gtggaagcc atctccaaa 
      gcgtgctac gaacgcatg ttccgctgg ttggtcact 
      cgaatcaac cgttcctta ggccgcact aaacgtcaa 
      ggtgccagt tttatcggc attcttgat attgccggt 
      tttgaaatc ttcgaactc aactctttc gaacaactg 
      tgtatcaac tacaccaat gaaaaattg caacaactc 
      ttcaaccac acgatgttc atccttgaa caagaagaa 
      tatcaacgt gaaggcatc gaatggaaa tttatagat 
      tttggactc gatctgcaa cccaccatt gacctcatc 
      gacaaacca atgggaatt atggcactt ttggacgaa 
      gagtgcctt ttcccgaaa gccaccgac aagactttc 
      gttgataaa ttggtcagt gcacactca gtgcatccc 
      aagttcaaa aagagtgat tttcggggt gttgccgat 
      ttttcgatc attcattac gccggtaaa gtagattac 
      tgtgctaat caatggttg atgaaaaat atggacccg 
      caaaatgag aatgtggtc tcgctgttg caagcgtcc 
      caagatccg ttcgttgtc cacatttgg aaagacgcc 
      gaaagtatc ggccgcgcc aaaggaatg tttagaact 
      gtttcgtat ttgtataaa gaacagttg gctaacctt 
      atggtgact ttgcgtaac accaatcca aacttcgta 
      cgttgtatt atccctaac cacgaaaaa cgggcggga 
      aaaatcgat gctcctttg gttttggac caactgagg 
      tgtaatggt gtccttgaa ggcatccga atttgccgg 
      caaggtttc cccaataga ataccattc caggagttt 
      aggcaaaga tacgaactt ttgacaccg aatgtcatt 
      aacaagggt ttcatggac ggtaagaag gcctgcgag 
      accatgatc aaaagtctg gaactggac cagaacttg 
      tacagaatc ggccaatcg aagattttc ttccgcgcc 
      ggtgttttg gcacatttg gaggaagaa cgcgattac 
      aaaatcacc gatttgatc gtaaatttc caagcgttc 
      tgtcgcggt ttcttgtcg cgaaggaat taccaaaaa 
      cgcgtccaa caattgaac gcaatccga atcattcaa 
      cgcaattgt tcggcttac ctcaagttg cgtaattgg 
      cagtggtgg cgcctctac accaaagtc aaacccttg 
      ctagaggtg accaaacaa gaggagaag ttgatgcaa 
      aaagaagac gaattgaag caagtcaaa gacaagtta 
      gagcaccac atcaaagcg gcaaaagaa ttcgaaacc 
      aagtaccaa caagcccaa gaagagaaa gtcatgctt 
      caggagcaa ctgcaagcc gaggtggaa ttgtgtgcc 
      gaggctgaa gaaatgaga gcgcgtcta acggcccgc 
      aagcaagaa ctcgaagag attcttcac gacttggag 
      gccaggatc gaggaggag gaagaaagg gcgaatact 
      ttgagtaac gataagaag aagttgcaa ttaacgatt 
      caagatttg gaagaacag ttggaggag gaggaaggt 
      gccaggcaa aaattgcag ttggaaaag gtccagtgt 
      gacgctaaa attaaaaaa ctggaagaa gacctggct 
      ttgagcgag gatacgaat caaaagttg ctcaaggag 
      aagaaggtt ttggaggaa cgtagcaac gacctgagc 
      caggctttg gccgaagaa gaagaaaaa gccaagcat 
      ttggcgaaa ctaaaagcc aaacacgag gcaaccatt 
      gccgaactg gaagaacgc ctccttaaa gaccaccaa 
      caacgccaa gaatccgac cgaaccaag cgcaaagtc 
      gaaacagaa gtcaacgac ttgaaggaa caacttgcc 
      gagaaaaaa tcacaactt gaagaattg caattacag 
      ttgggcaaa agagaggag gagttggcc caggctatg 
      gtccgagtc gacgaagaa ggcgccctt aaggcccaa 
      gcccagaag gccctccgg gagatggaa agccaactc 
      agcgaacta caagaggat ttagaagcg gaaaaagcc 
      gccagaagc aaggccgaa aaattaaaa cgcgaccta 
      aacgaagaa cttgaagcg ttgaaaaac gaactactt 
      gactcactc gacaccaca gccgcccaa caagaattg 
      cgttcaaaa cgtgaacaa gaactcgcc aatttgaag 
      aagaacttg gaagaggag aaccaagtc catgaagcg 
      actttggcc gacatgcgc cacaaacac acccaagaa 
      ctggcttcc ttgaacgaa caattggaa aatatcaag 
      aaacagaaa gccagtatc gaaaaagcg aagcaagga 
      ctcgaagcc gaaaacgcc gacttgaca agcgagttg 
      cgaaatgtc ggagcgagt cgacaagaa ggcgaacgg 
      cgaagaaaa caagccgag agccaattg gtcgaaatc 
      caaggcaag ctgggcgaa attgaacga gctcgagcc 
      gaacttgcc gaaaaaaat caaaaacta caacaggag 
      atggacaat gtggtccaa cagctggag gaagccgaa 
      ctgaaagcg tccgcagct gtgaagaat caaggaact 
      atcgaatcg caatttgcc gaggcacaa agtgccctg 
      gaggaggag acacgccag aagttggct ttgagttcg 
      aaactgcgc caattggag tcggacaag gagaatttg 
      caagaacag atcgaggag gaggaagaa gcgaagaaa 
      aatctcgag aaacaattg aacgcggtt atggtccaa 
      ctggccgaa gcgaagaaa aaggccgaa gatgaggcc 
      gaacaatcg gcgattttg gaagaaagc aagaagaaa 
      ctggccaaa gatttggag ttgttgcaa cgtcaagtc 
      gaggaattg accgcggcc aacgacaag ttggagcga 
      agcaagaag aagatcgcc gccgaactg gaagatact 
      aacattgat ttggaggcg cagcgccag aaagtggcc 
      gagctggag aagaagcag aagaatttc gataaagtc 
      ttggccgaa gaaaagcaa gtcagtgag caactgatg 
      gcggaaaaa gacgccgct gagcgagaa gcccgcgag 
      aaggaaact cgagtcttg tcactgagc cgcgaactg 
      gacgaatcc aacgtcaaa gttgaagaa ctggaacgc 
      atcaagcgc caacttcag gccgaactg gacgaactg 
      gtcaataat caaggcacc gccgacaag aacgtgcac 
      gagttggag aaagccaaa cgttcgctt gaaacccaa 
      ctggcggaa ctgaaagcc cagaacgaa gaactggaa 
      gatgagcta caactcacc gaagatgca aaactccga 
      cttgaggtg aacatgcaa gcattgcgg gcgcaattc 
      gaacgcgac gtccaagcc aaggaggag caagccgag 
      gagaaacgc cgcggaatc gtcaaacaa ttgcgcgat 
      ttggaggcc gaactggac gaagaacgg aagcaaaga 
      gcggccgca gtcaccgct cgaaagaaa ctcgaaggc 
      gatttgaaa gaactcgaa gctcagatc gaaatgcaa 
      tgcaagatg aaagaagac gcgttgaaa cagttgaag 
      aagtcgcaa cagcagtgc aaggaggcg gtgcgagac 
      gccgaagaa gcgcgcgct tcgcgcgac gaactcgcc 
      gccgggtgt aaagaagcc gaacgtaaa gttaaagca 
      ctcgaagct gaggtgctt caattgagc gaagatcac 
      gccagttcg gagcgcgct cgtcgagct gccgaagcc 
      gaacgcgat gaactgctc gaagaggtt aataatacg 
      aacagtaag ggggcgttg ttgatcgat gagaagcgg 
      cgtcttgaa gctaggatt gccacgctt gaggaggag 
      attgaagag gagcagagt aacaatgaa ctgttgcaa 
      gaccgggca aggaaggca cagttgact attgagcaa 
      ttgacgagt gagttggcc acggaacga tcactggct 
      cagaaacaa gagtcgggt aaattgctg ctcgagaga 
      cagaataag gagttgaag gccaagttg agtgagttg 
      gagacggca caaaggacc aagacgaag gcgacgatc 
      gcggcgttg gagagcaag attgggaat ttggaggag 
      cagttggag gtcgaggcc aaggagagg ttggcgcag 
      caaaagacc aaccggaag ttggacaag aagttgaag 
      gagttggtc atgcagttg gaggatgag cggagacat 
      gccgaccag tataaggag caagtggat aaggcgaac 
      gcacgggtt aagacgctg aagcggcag ttggatgag 
      gccgaggaa gagataacg agggagaag gcgcagaag 
      aggaaagtg caaagggat tatgaggat atgctggag 
      agtaatgaa tcaatgacg cgggagctt aacagtctt 
      aagaccaaa ctgaggcgc gggactggg gctagtagc 
      ggcatgggt ctgcccaga ctcagcggt cggggctcc 
      atccagtcc gggggcaac ggctccggt gacgattcc 
      acaacccag gatgacgcc atagacggc gaagacgcc 
      cccaattag
    mRNA sequence
    • >TC034132 RA
      tcattctgt aaatcgcat tcaaccggt cgcgttcag 
      cagccccgt aagcccccg atgtgcttt tagtgttgt 
      ctgtgcgca gtgtttagg ccatggcgg acgccgaca 
      gcaaggacc gctacgatc ccgaactga agtacctct 
      gcgtggacc ggaacaact tcaacgacc cggccaccc 
      aggccgagt ggacccaga agcggctgg tatgggtgc 
      cgcacgagg cgcagggct tcgtcgccg ccagcatca 
      agggcgagc gcggcgacg aggtcgagg tggagctcc 
      aggagacgg ggaagcgca ccaccgtcg ccagagacg 
      acatccaga agatgaacc cgcccaagt tcgacaagg 
      tcgaggaca tggcggaac tcacctgcc tcaacgagg 
      cctgtgtct tgcacaact tgaaagaca gatactatt 
      cgggactga tttatactt actccggcc tattttgcg 
      tcgttgtca acccctaca aaaagctcc ctatttaca 
      cagaaaaaa ttatggaac gctacaagg gcattaagc 
      gacacgaag tgcctcctc atgtctttg ccataaccg 
      acacggcct atcgttcga tgttacaag agcgagaag 
      accagtcta tactttgta cgggcgagt caggtgccg 
      gtaaaacag aaaacacga aaaaagtca ttcagtatt 
      tggcctatg ttgccgcct cgaagtcac caaaaggct 
      caggagcgc agaaagacc ttattggcg gtttggagc 
      aacaactgt tacaagcca atccgatcc ttgaagctt 
      ttggtaacg caaaaacaa tcaaaaacg acaattctt 
      ccagatttg gaaaattca ttcggataa atttcgacg 
      cttccggtt acatagccg gtgcaaaca tcgagactt 
      accttcttg aaaaatcac gtgccatac gacaagcca 
      agcaggaac gaaccttcc atatcttct accaactat 
      tggctggag cttccgccg agcagaaaa aggagttta 
      ttctcgagg acccaaaat cgtacccat tccttcgtg 
      aagacaatc acatagtgc ccggagtgg acgattcgg 
      ccgagttcc aggccaccg tcaagagta tgaatataa 
      tggggatga cgaacgaag atttcagtg ccattttcc 
      gcgttgttt cggccgtaa tgctcttcg gaacgatgc 
      aattcaaac aggatcgta attcggatc aggcgacgt 
      tacccgaca acacggtgg cgcaaaaaa tcgcccatt 
      tgctgggtc tgtctgtaa ctgacatga ccaaggctt 
      tcctcaaac ccaggatta aggtcggga gggatttcg 
      tcacgaaga gccaaacga aagaacagg tggaattcg 
      ctgtggaag ccatctcca aagcgtgct acgaacgca 
      tgttccgct ggttggtca ctcgaatca accgttcct 
      taggccgca ctaaacgtc aaggtgcca gttttatcg 
      gcattcttg atattgccg gttttgaaa tcttcgaac 
      tcaactctt tcgaacaac tgtgtatca actacacca 
      atgaaaaat tgcaacaac tcttcaacc acacgatgt 
      tcatccttg aacaagaag aatatcaac gtgaaggca 
      tcgaatgga aatttatag attttggac tcgatctgc 
      aacccacca ttgacctca tcgacaaac caatgggaa 
      ttatggcac ttttggacg aagagtgcc ttttcccga 
      aagccaccg acaagactt tcgttgata aattggtca 
      gtgcacact cagtgcatc ccaagttca aaaagagtg 
      attttcggg gtgttgccg atttttcga tcattcatt 
      acgccggta aagtagatt actgtgcta atcaatggt 
      tgatgaaaa atatggacc cgcaaaatg agaatgtgg 
      tctcgctgt tgcaagcgt cccaagatc cgttcgttg 
      tccacattt ggaaagacg ccgaaagta tcggccgcg 
      ccaaaggaa tgtttagaa ctgtttcgt atttgtata 
      aagaacagt tggctaacc ttatggtga ctttgcgta 
      acaccaatc caaacttcg tacgttgta ttatcccta 
      accacgaaa aacgggcgg gaaaaatcg atgctcctt 
      tggttttgg accaactga ggtgtaatg gtgtccttg 
      aaggcatcc gaatttgcc ggcaaggtt tccccaata 
      gaataccat tccaggagt ttaggcaaa gatacgaac 
      ttttgacac cgaatgtca ttaacaagg gtttcatgg 
      acggtaaga aggcctgcg agaccatga tcaaaagtc 
      tggaactgg accagaact tgtacagaa tcggccaat 
      cgaagattt tcttccgcg ccggtgttt tggcacatt 
      tggaggaag aacgcgatt acaaaatca ccgatttga 
      tcgtaaatt tccaagcgt tctgtcgcg gtttcttgt 
      cgcgaagga attaccaaa aacgcgtcc aacaattga 
      acgcaatcc gaatcattc aacgcaatt gttcggctt 
      acctcaagt tgcgtaatt ggcagtggt ggcgcctct 
      acaccaaag tcaaaccct tgctagagg tgaccaaac 
      aagaggaga agttgatgc aaaaagaag acgaattga 
      agcaagtca aagacaagt tagagcacc acatcaaag 
      cggcaaaag aattcgaaa ccaagtacc aacaagccc 
      aagaagaga aagtcatgc ttcaggagc aactgcaag 
      ccgaggtgg aattgtgtg ccgaggctg aagaaatga 
      gagcgcgtc taacggccc gcaagcaag aactcgaag 
      agattcttc acgacttgg aggccagga tcgaggagg 
      aggaagaaa gggcgaata ctttgagta acgataaga 
      agaagttgc aattaacga ttcaagatt tggaagaac 
      agttggagg aggaggaag gtgccaggc aaaaattgc 
      agttggaaa aggtccagt gtgacgcta aaattaaaa 
      aactggaag aagacctgg ctttgagcg aggatacga 
      atcaaaagt tgctcaagg agaagaagg ttttggagg 
      aacgtagca acgacctga gccaggctt tggccgaag 
      aagaagaaa aagccaagc atttggcga aactaaaag 
      ccaaacacg aggcaacca ttgccgaac tggaagaac 
      gcctcctta aagaccacc aacaacgcc aagaatccg 
      accgaacca agcgcaaag tcgaaacag aagtcaacg 
      acttgaagg aacaacttg ccgagaaaa aatcacaac 
      ttgaagaat tgcaattac agttgggca aaagagagg 
      aggagttgg cccaggcta tggtccgag tcgacgaag 
      aaggcgccc ttaaggccc aagcccaga aggccctcc 
      gggagatgg aaagccaac tcagcgaac tacaagagg 
      atttagaag cggaaaaag ccgccagaa gcaaggccg 
      aaaaattaa aacgcgacc taaacgaag aacttgaag 
      cgttgaaaa acgaactac ttgactcac tcgacacca 
      cagccgccc aacaagaat tgcgttcaa aacgtgaac 
      aagaactcg ccaatttga agaagaact tggaagagg 
      agaaccaag tccatgaag cgactttgg ccgacatgc 
      gccacaaac acacccaag aactggctt ccttgaacg 
      aacaattgg aaaatatca agaaacaga aagccagta 
      tcgaaaaag cgaagcaag gactcgaag ccgaaaacg 
      ccgacttga caagcgagt tgcgaaatg tcggagcga 
      gtcgacaag aaggcgaac ggcgaagaa aacaagccg 
      agagccaat tggtcgaaa tccaaggca agctgggcg 
      aaattgaac gagctcgag ccgaacttg ccgaaaaaa 
      atcaaaaac tacaacagg agatggaca atgtggtcc 
      aacagctgg aggaagccg aactgaaag cgtccgcag 
      ctgtgaaga atcaaggaa ctatcgaat cgcaatttg 
      ccgaggcac aaagtgccc tggaggagg agacacgcc 
      agaagttgg ctttgagtt cgaaactgc gccaattgg 
      agtcggaca aggagaatt tgcaagaac agatcgagg 
      aggaggaag aagcgaaga aaaatctcg agaaacaat 
      tgaacgcgg ttatggtcc aactggccg aagcgaaga 
      aaaaggccg aagatgagg ccgaacaat cggcgattt 
      tggaagaaa gcaagaaga aactggcca aagatttgg 
      agttgttgc aacgtcaag tcgaggaat tgaccgcgg 
      ccaacgaca agttggagc gaagcaaga agaagatcg 
      ccgccgaac tggaagata ctaacattg atttggagg 
      cgcagcgcc agaaagtgg ccgagctgg agaagaagc 
      agaagaatt tcgataaag tcttggccg aagaaaagc 
      aagtcagtg agcaactga tggcggaaa aagacgccg 
      ctgagcgag aagcccgcg agaaggaaa ctcgagtct 
      tgtcactga gccgcgaac tggacgaat ccaacgtca 
      aagttgaag aactggaac gcatcaagc gccaacttc 
      aggccgaac tggacgaac tggtcaata atcaaggca 
      ccgccgaca agaacgtgc acgagttgg agaaagcca 
      aacgttcgc ttgaaaccc aactggcgg aactgaaag 
      cccagaacg aagaactgg aagatgagc tacaactca 
      ccgaagatg caaaactcc gacttgagg tgaacatgc 
      aagcattgc gggcgcaat tcgaacgcg acgtccaag 
      ccaaggagg agcaagccg aggagaaac gccgcggaa 
      tcgtcaaac aattgcgcg atttggagg ccgaactgg 
      acgaagaac ggaagcaaa gagcggccg cagtcaccg 
      ctcgaaaga aactcgaag gcgatttga aagaactcg 
      aagctcaga tcgaaatgc aatgcaaga tgaaagaag 
      acgcgttga aacagttga agaagtcgc aacagcagt 
      gcaaggagg cggtgcgag acgccgaag aagcgcgcg 
      cttcgcgcg acgaactcg ccgccgggt gtaaagaag 
      ccgaacgta aagttaaag cactcgaag ctgaggtgc 
      ttcaattga gcgaagatc acgccagtt cggagcgcg 
      ctcgtcgag ctgccgaag ccgaacgcg atgaactgc 
      tcgaagagg ttaataata cgaacagta agggggcgt 
      tgttgatcg atgagaagc ggcgtcttg aagctagga 
      ttgccacgc ttgaggagg agattgaag aggagcaga 
      gtaacaatg aactgttgc aagaccggg caaggaagg 
      cacagttga ctattgagc aattgacga gtgagttgg 
      ccacggaac gatcactgg ctcagaaac aagagtcgg 
      gtaaattgc tgctcgaga gacagaata aggagttga 
      aggccaagt tgagtgagt tggagacgg cacaaagga 
      ccaagacga aggcgacga tcgcggcgt tggagagca 
      agattggga atttggagg agcagttgg aggtcgagg 
      ccaaggaga ggttggcgc agcaaaaga ccaaccgga 
      agttggaca agaagttga aggagttgg tcatgcagt 
      tggaggatg agcggagac atgccgacc agtataagg 
      agcaagtgg ataaggcga acgcacggg ttaagacgc 
      tgaagcggc agttggatg aggccgagg aagagataa 
      cgagggaga aggcgcaga agaggaaag tgcaaaggg 
      attatgagg atatgctgg agagtaatg aatcaatga 
      cgcgggagc ttaacagtc ttaagacca aactgaggc 
      gcgggactg gggctagta gcggcatgg gtctgccca 
      gactcagcg gtcggggct ccatccagt ccgggggca 
      acggctccg gtgacgatt ccacaaccc aggatgacg 
      ccatagacg gcgaagacg cccccaatt aggtcgcat 
      ttttactca ttcggttgt aaatatatt tgtatatcc 
      cttattagt acttagcat ttttaatat gcgcatttt 
      gtggattat ttgtaaaaa tcggacagt cagttcggc 
      gcttaatca gtatcagta atcaagttc attattttt 
      tttatttat agttttttt attaatttt aatatcgtg 
      ctttatagt gattaattt gtactttta acagttgat 
      tgcatttat ttatatgaa agtctactt gcttaattt 
      gtttctagg gtttcatcg tactttacc ttttataca 
      tttaggaat tagttggaa gcatcagca tttaaataa 
      catttatgt atctattaa taataataa tcaataatc 
      gctaacgac aaatgacat tttttatac aacatttat 
      aggattata tattttgta aaaaaatcc cgctagcat 
      cgaggaaat tttgatatt tgacgtttt tgtataaaa 
      aatttcagg tttttgtat gtatatatc cttttcatg 
      tataaaaaa tatatacat attttgggt ttcgggctg 
      atttatttg tactttaac gagttcaat aaaatcgta 
      aaaaagtc
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