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Gene information
General information
TC005512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11885711..11892979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005512
    iBeetle screen iB_03971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection


    Adult morphology
    elytra dorsal orientation not closed - Number of animals showing the phenotype: 7


    elytra surface irregular - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005512 RA
      MFRSRSWFG GGWGRPKNP HSLEHLKYL YNVLSRNQT 
      VSEHNRGLL VETLRSIAE ILIWGDQND SSVFDFFLE 
      KNMLSFFLR IMRQRSGGS SFVCVQLLQ TLNILFENI 
      RNETSLYYL LSNNHVNSI IVHKFDFSD EEVMAYYIS 
      FLKTLSLKL NAHTIHFFY NEHTNDFPL YTEAIKFFN 
      HPESMVRIA VRTLTLNVY KVEDASMLA FIRDRTAAP 
      YFSNIVWFI GKHIQELDA CVRNDADHQ SQNRLSDLV 
      AEHLDHLHY LNDILCLNI TDLNQVLTD HLLHKLLIP 
      LYIYSLSTV RKPTGGVEA IAKALNQKL NLNESSSSS 
      NGGISHEPS TGKVSCVVA LFLLSQVCL IISHSPVVQ 
      TLAWIILKS DRSIYDKGV EKLLEAYEL EMKQNGKKE 
      EASGSENSF KDDVIEEHK NITDEEKQR LAITPTESE 
      SVSEKPFLE TVYNALDCS ENDYAALFA LTLLYAMAY 
      NKGIMPDIN EQVLRPKKT YTSKTLSRD VRYTYNTYL 
      VDKLLYIVL LSCQPSCRV RLVTLELAL KLLVQLVMA 
      DGKSVLLES HKLTIESAK TQSTALLRN FYKSEEIFL 
      DMFEHEYCD MSKTSLNVE WLCMDSAIL LPPTGTPMT 
      GIDFTKRLP CGEVERARR AIRVFFLVR NVVLTLNGE 
      LETQLPLTN PQSCVQIEQ ALDLSISRY NSDLIACTV 
      LWKDGTKTR RFLVIDVLQ LILVEPDTK RLGWGVAKL 
      VGFLQDIEV TGDKDDSRC LHITIHRPL SGTTTNRVP 
      LLSAKFVFD DNIRCMAAK QRLTKGRIK ARQKKMQHI 
      ARLLEIPGQ SGPPSPAFV GGGLYNRST GRGLRDRPF 
      ALSRGPGFA APRWESPPG VTHRTDESR RHRDSDPKI 
      LKKEDRTPN RSRDSSSSR TKSREGSPR MPRPRSEEI 
      PLELIKKAS NSVIDMAPK IAIVPRPQV KEESEEVEK 
      KPEEEVSIS SEETSFIAS ERRNSKKDG NVETV
    CDS sequence
    • >TC005512 RA
      atgttccgt agtagaagc tggttcggt ggtggctgg 
      ggccgcccc aagaacccc cattcgctg gaacacttg 
      aaataccta tacaacgtg ttgtctcgg aaccaaaca 
      gtgtcggaa cacaaccgg ggtttgctt gtggaaacc 
      cttcggtcc atagccgaa attctaatc tggggcgac 
      caaaatgac tcgtccgtt tttgacttc tttttggag 
      aagaacatg ttgtcgttc tttctgcgt atcatgaga 
      caacgaagc gggggctcg agctttgtc tgcgtccag 
      ttactccaa acgcttaat atcctattc gaaaatatt 
      agaaacgaa acttcgcta tattacttg ttatcaaat 
      aatcacgtt aattcgata attgtgcac aagttcgat 
      ttttcggac gaggaagta atggcgtac tatatctcc 
      tttttgaaa actttgagc ttgaaactg aacgctcat 
      acgatacat tttttctat aatgaacat actaatgat 
      tttccgttg tatacggaa gcgatcaag ttttttaat 
      caccctgag tctatggtc aggatagca gtacgtact 
      ttgacgctg aatgtttac aaagttgag gacgcaagt 
      atgcttgcg ttcatcaga gacagaacc gcagctccg 
      tatttttcc aacattgtt tggttcatt ggcaaacat 
      atccaagag ttggatgct tgtgtccgc aacgatgcc 
      gatcatcag tcgcagaat cgcttgtct gacctcgta 
      gcagaacac ttggaccac ctccactac ttaaacgac 
      atcctttgc ttgaacatc accgatttg aaccaagtc 
      ctcaccgac cacctctta cacaaactc ctcatccct 
      ttatacatc tactcgctg tcaacggtg cgaaagccc 
      accggaggt gtggaagca atcgcaaaa gcgctcaat 
      caaaaactg aacttaaac gaatcatca tcatcatca 
      aacggtggg atttcgcac gaaccctca accgggaag 
      gtttcatgc gttgtcgct ttgttcctc ctgtcgcaa 
      gtttgtctc atcatttcg cactcgccg gttgtccaa 
      acccttgcc tggataatc ctcaaaagc gatcgctcg 
      atttacgat aaaggcgtc gagaaattg ctagaagcg 
      tacgaactt gaaatgaaa caaaacggg aaaaaggaa 
      gaagccagt gggtctgaa aacagtttc aaggacgat 
      gtaattgag gaacataaa aacatcacc gatgaagag 
      aaacaacga ttggcgata acgccgact gagagtgaa 
      agtgtgtcg gaaaaaccg tttttggaa acggtttac 
      aacgcattg gactgttcg gaaaacgac tacgctgcg 
      ctttttgcc ctcacttta ctgtacgcc atggcatac 
      aataaaggg ataatgccg gacataaac gaacaagta 
      ttaaggcct aaaaagacc tacacgtcg aagacttta 
      tctcgcgat gtccgctac acttataac acctatctg 
      gtggacaaa ctcttgtac atagtgctg ctgtcgtgt 
      cagccttcg tgtcgcgtc cgtttggtg acgcttgag 
      ttggctttg aagcttctg gtgcaacta gtgatggct 
      gatggtaaa agtgtactt cttgagtcg cataaactg 
      acgatagag tcggcgaaa acgcaaagt acggcgctc 
      ttgaggaat ttttacaag agtgaggag atttttctc 
      gatatgttt gaacatgaa tactgtgat atgtcgaag 
      acttcgttg aatgttgaa tggttgtgt atggatagc 
      gcgattttg ttgccgcca acgggcaca cccatgact 
      gggattgat ttcaccaag aggttgccc tgcggagag 
      gtcgagcgg gcgcggcga gccatccgc gtgtttttc 
      ctggtgcga aacgtggta ttgacgctg aacggcgag 
      ctggaaacc caactgccg ctcaccaac ccccagagc 
      tgcgtccag attgagcaa gccttggac ctgagtata 
      tctcgttat aattccgat ttgatcgcg tgtacggtg 
      ctttggaaa gacgggacg aagacgagg cgatttctg 
      gtcattgat gtcttgcag ctgattctg gttgaacca 
      gatacgaag aggttgggc tggggggtg gccaagttg 
      gttgggttt cttcaagat attgaagtg actggggat 
      aaggatgat tctaggtgt ctgcatatt acgatacat 
      aggcctttg tccgggact acgaccaat cgggtgcca 
      ttgctcagt gccaagttt gttttcgac gataatatc 
      aggtgtatg gcggcaaag cagagatta acaaagggc 
      cgtatcaag gctcgtcaa aagaaaatg cagcacatc 
      gcccgcctc ctcgaaatc cccggtcag tcggggccc 
      ccaagtccc gccttcgtc ggcgggggc ctctacaac 
      cgctccacg gggcgaggc ctccgcgac cgccccttc 
      gccctctcc cgggggccc ggttttgcc gccccccgc 
      tgggagagc cccccaggt gtgacacac cgaacggac 
      gaatcgcgc cgtcatcgt gacagtgat ccaaaaatt 
      ttgaaaaag gaagatcgc actcccaac aggtccaga 
      gattcgtca tcgagtcgg acgaagtcc agggagggt 
      tcccccagg atgcccagg ccacggtcg gaggagatc 
      ccgctggag ttgatcaag aaggcgtcg aattcggtg 
      attgatatg gcgcccaaa atcgcaatt gtgccaagg 
      ccgcaagtg aaggaagaa agtgaagaa gtggagaag 
      aaaccggag gaggaagtt tcgatcagt tcggaagaa 
      acgtcgttt attgcgagt gagaggagg aattcgaag 
      aaagacggg aatgtggag acggtatag
    mRNA sequence
    • >TC005512 RA
      ttgacgtta acaaacatc tgtcaaacg tgccctgtc 
      caaatcaaa actaattaa attatcgca aaaatgtcg 
      caaacccgt ggttatggt gcttaaaac tgtgcaatg 
      ttccgtagt agaagctgg ttcggtggt ggctggggc 
      cgccccaag aacccccat tcgctggaa cacttgaaa 
      tacctatac aacgtgttg tctcggaac caaacagtg 
      tcggaacac aaccggggt ttgcttgtg gaaaccctt 
      cggtccata gccgaaatt ctaatctgg ggcgaccaa 
      aatgactcg tccgttttt gacttcttt ttggagaag 
      aacatgttg tcgttcttt ctgcgtatc atgagacaa 
      cgaagcggg ggctcgagc tttgtctgc gtccagtta 
      ctccaaacg cttaatatc ctattcgaa aatattaga 
      aacgaaact tcgctatat tacttgtta tcaaataat 
      cacgttaat tcgataatt gtgcacaag ttcgatttt 
      tcggacgag gaagtaatg gcgtactat atctccttt 
      ttgaaaact ttgagcttg aaactgaac gctcatacg 
      atacatttt ttctataat gaacatact aatgatttt 
      ccgttgtat acggaagcg atcaagttt tttaatcac 
      cctgagtct atggtcagg atagcagta cgtactttg 
      acgctgaat gtttacaaa gttgaggac gcaagtatg 
      cttgcgttc atcagagac agaaccgca gctccgtat 
      ttttccaac attgtttgg ttcattggc aaacatatc 
      caagagttg gatgcttgt gtccgcaac gatgccgat 
      catcagtcg cagaatcgc ttgtctgac ctcgtagca 
      gaacacttg gaccacctc cactactta aacgacatc 
      ctttgcttg aacatcacc gatttgaac caagtcctc 
      accgaccac ctcttacac aaactcctc atcccttta 
      tacatctac tcgctgtca acggtgcga aagcccacc 
      ggaggtgtg gaagcaatc gcaaaagcg ctcaatcaa 
      aaactgaac ttaaacgaa tcatcatca tcatcaaac 
      ggtgggatt tcgcacgaa ccctcaacc gggaaggtt 
      tcatgcgtt gtcgctttg ttcctcctg tcgcaagtt 
      tgtctcatc atttcgcac tcgccggtt gtccaaacc 
      cttgcctgg ataatcctc aaaagcgat cgctcgatt 
      tacgataaa ggcgtcgag aaattgcta gaagcgtac 
      gaacttgaa atgaaacaa aacgggaaa aaggaagaa 
      gccagtggg tctgaaaac agtttcaag gacgatgta 
      attgaggaa cataaaaac atcaccgat gaagagaaa 
      caacgattg gcgataacg ccgactgag agtgaaagt 
      gtgtcggaa aaaccgttt ttggaaacg gtttacaac 
      gcattggac tgttcggaa aacgactac gctgcgctt 
      tttgccctc actttactg tacgccatg gcatacaat 
      aaagggata atgccggac ataaacgaa caagtatta 
      aggcctaaa aagacctac acgtcgaag actttatct 
      cgcgatgtc cgctacact tataacacc tatctggtg 
      gacaaactc ttgtacata gtgctgctg tcgtgtcag 
      ccttcgtgt cgcgtccgt ttggtgacg cttgagttg 
      gctttgaag cttctggtg caactagtg atggctgat 
      ggtaaaagt gtacttctt gagtcgcat aaactgacg 
      atagagtcg gcgaaaacg caaagtacg gcgctcttg 
      aggaatttt tacaagagt gaggagatt tttctcgat 
      atgtttgaa catgaatac tgtgatatg tcgaagact 
      tcgttgaat gttgaatgg ttgtgtatg gatagcgcg 
      attttgttg ccgccaacg ggcacaccc atgactggg 
      attgatttc accaagagg ttgccctgc ggagaggtc 
      gagcgggcg cggcgagcc atccgcgtg tttttcctg 
      gtgcgaaac gtggtattg acgctgaac ggcgagctg 
      gaaacccaa ctgccgctc accaacccc cagagctgc 
      gtccagatt gagcaagcc ttggacctg agtatatct 
      cgttataat tccgatttg atcgcgtgt acggtgctt 
      tggaaagac gggacgaag acgaggcga tttctggtc 
      attgatgtc ttgcagctg attctggtt gaaccagat 
      acgaagagg ttgggctgg ggggtggcc aagttggtt 
      gggtttctt caagatatt gaagtgact ggggataag 
      gatgattct aggtgtctg catattacg atacatagg 
      cctttgtcc gggactacg accaatcgg gtgccattg 
      ctcagtgcc aagtttgtt ttcgacgat aatatcagg 
      tgtatggcg gcaaagcag agattaaca aagggccgt 
      atcaaggct cgtcaaaag aaaatgcag cacatcgcc 
      cgcctcctc gaaatcccc ggtcagtcg gggccccca 
      agtcccgcc ttcgtcggc gggggcctc tacaaccgc 
      tccacgggg cgaggcctc cgcgaccgc cccttcgcc 
      ctctcccgg gggcccggt tttgccgcc ccccgctgg 
      gagagcccc ccaggtgtg acacaccga acggacgaa 
      tcgcgccgt catcgtgac agtgatcca aaaattttg 
      aaaaaggaa gatcgcact cccaacagg tccagagat 
      tcgtcatcg agtcggacg aagtccagg gagggttcc 
      cccaggatg cccaggcca cggtcggag gagatcccg 
      ctggagttg atcaagaag gcgtcgaat tcggtgatt 
      gatatggcg cccaaaatc gcaattgtg ccaaggccg 
      caagtgaag gaagaaagt gaagaagtg gagaagaaa 
      ccggaggag gaagtttcg atcagttcg gaagaaacg 
      tcgtttatt gcgagtgag aggaggaat tcgaagaaa 
      gacgggaat gtggagacg gtatagtac agggcggtt 
      ttgttgact ttagtgtaa ttgattaat aaaattgtt 
      ttatcatga tgattgttt gattgtgta attggtgat 
      aatcaaaat gttccctag gaaggtgag agggaggtc 
      tagctgcct ggaatctta tttttgttg aaattataa 
      acaattttt aataaactt atttttctt tttttgttt 
      tatttattt ttttatttt tcttttttt tttgggggg 
      gggnnnnnn nnnnnnnnn nnnnnnnnn tttaataaa 
      ctatttt
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