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Gene information
General information
TC005504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12023035..12026059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005504
    iBeetle screen iB_03970

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005504 RA
      MTTFRILTK FSRHFCSKP QKHVQVCIL GADTLLGQS 
      LAFLIKQNP AISGLHLQG TSKVESMAL DFNHFDTRC 
      RVHSYYDMD SVSKSVKCA DIVVMLGLN TSTSKMSIP 
      KLVMAEGVR VAKLAETCA KYAPKAVIV VAVTPISVT 
      LPIVAEVYK QSDWYHPGR LLGSAALAE VKANAIAGH 
      YQTLDPQMV HVPIVGGPD LDCAIPLFS QTQPVGIAQ 
      KDTDKLVEE FRKEIPQPA MSHAVGLNR MITGIATGI 
      NGDYNANVL AFVRTNIIK TCRYLVCIV KMGKMGILH 
      NYGLQKLRR NELITFEET CLQLKAREE MASDLVNRE 
      DKCALPAFM IREMEREKI EKLGIIR
    CDS sequence
    • >TC005504 RA
      atgacaacc tttcgcatc ttaacaaaa ttctcgcgc 
      cacttttgc tccaaaccg cagaaacac gtccaagta 
      tgcatcctt ggcgccgac acactctta ggccaatcc 
      cttgccttc ctcatcaaa caaaaccct gcaatttct 
      ggtctacac ctccagggc acttcaaag gtcgaatct 
      atggctctg gacttcaac cactttgac accagatgc 
      cgagttcac tcttattac gacatggac agcgtcagc 
      aaatccgtc aagtgtgcc gatattgtg gtcatgctg 
      gggctcaac acaagcacc agcaaaatg tccataccc 
      aagctggtc atggctgaa ggcgtccga gtggccaaa 
      ctggccgaa acctgcgca aaatacgcc ccgaaagct 
      gtaattgta gtggcagtg acgcccatt tcggtcact 
      ttgcccatc gttgctgag gtgtataaa cagtcggat 
      tggtaccat cctgggcgg cttcttggt tcggctgct 
      ctggccgaa gtcaaagcc aacgctatt gctggacat 
      taccagacg ctggatccg caaatggtg catgtgccc 
      attgttgga ggtccagat ttggactgc gctatccct 
      cttttttcc caaactcaa ccagtggga atagcccag 
      aaagataca gataagttg gtcgaggaa ttccggaag 
      gaaatccca caaccagcg atgagtcac gcagtgggg 
      cttaatagg atgatcaca gggattgcc acagggatt 
      aacggggat tataacgcc aatgtcttg gcttttgtg 
      cgaacgaat attatcaag acgtgtcga tatttggtg 
      tgcatcgta aaaatgggc aaaatgggc attttgcac 
      aactacggt ttgcagaag ctgcggcgc aatgaattg 
      atcacattc gaagaaacg tgcttgcag ctcaaagct 
      cgggaagaa atggcttcg gatctggta aatcgcgaa 
      gataaatgc gcccttcct gcattcatg atccgggag 
      atggagcga gaaaaaatc gaaaaactt ggaattata 
      cgataa
    mRNA sequence
    • >TC005504 RA
      tttgtcata gtgaaagat tttatttat taaaaaaat 
      tcataaccc gaaaactaa aagtcgtag agcaatgcg 
      gtttgttcc tttgaattc agcggctta ttttctgta 
      ttatacgaa cttttcatg aaatttaga aattaaatt 
      ttttaaata agaagaaat cttttgaga atgacaata 
      atttgcgtc aaaattctg acaatgaca acctttcgc 
      atcttaaca aaattctcg cgccacttt tgctccaaa 
      ccgcagaaa cacgtccaa gtatgcatc cttggcgcc 
      gacacactc ttaggccaa tcccttgcc ttcctcatc 
      aaacaaaac cctgcaatt tctggtcta cacctccag 
      ggcacttca aaggtcgaa tctatggct ctggacttc 
      aaccacttt gacaccaga tgccgagtt cactcttat 
      tacgacatg gacagcgtc agcaaatcc gtcaagtgt 
      gccgatatt gtggtcatg ctggggctc aacacaagc 
      accagcaaa atgtccata cccaagctg gtcatggct 
      gaaggcgtc cgagtggcc aaactggcc gaaacctgc 
      gcaaaatac gccccgaaa gctgtaatt gtagtggca 
      gtgacgccc atttcggtc actttgccc atcgttgct 
      gaggtgtat aaacagtcg gattggtac catcctggg 
      cggcttctt ggttcggct gctctggcc gaagtcaaa 
      gccaacgct attgctgga cattaccag acgctggat 
      ccgcaaatg gtgcatgtg cccattgtt ggaggtcca 
      gatttggac tgcgctatc cctcttttt tcccaaact 
      caaccagtg ggaatagcc cagaaagat acagataag 
      ttggtcgag gaattccgg aaggaaatc ccacaacca 
      gcgatgagt cacgcagtg gggcttaat aggatgatc 
      acagggatt gccacaggg attaacggg gattataac 
      gccaatgtc ttggctttt gtgcgaacg aatattatc 
      aagacgtgt cgatatttg gtgtgcatc gtaaaaatg 
      ggcaaaatg ggcattttg cacaactac ggtttgcag 
      aagctgcgg cgcaatgaa ttgatcaca ttcgaagaa 
      acgtgcttg cagctcaaa gctcgggaa gaaatggct 
      tcggatctg gtaaatcgc gaagataaa tgcgccctt 
      cctgcattc atgatccgg gagatggag cgagaaaaa 
      atcgaaaaa cttggaatt atacgataa taacggtgt 
      aacggcgcc aataaatcc aatttgtga at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY