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Gene information
General information
TC005503 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12036587..12043539
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005503
    iBeetle screen iB_03969

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005503 RA
      MNVFTQVFG VALNFLMIP FNPSAPPMP YIVSETTCR 
      ITNFKFGEN EMKVSLAPR GYCPNCCPV KSGVDVRFV 
      LYTKSTPDR GAIIAPSPG AARRAGVDP LAPTVIYIH 
      GFSEPSPGK SGRGIAHAY LSRRDNFNV ILLDWSDLS 
      TFPWYLPAV RNVKIVAEK LRKFLEVFN DSGEIPLGN 
      VHLIGFSLG SHIAGFAGK QLRRGLRIP RITALDPAF 
      PEYSLNDAS RRLTRTDAD YIDVIHTDA GVLGLPISV 
      GHADFYPNG GRALQPGCQ PSYLVQLRL VDQIFACSH 
      VRAWRLYAE SVMHPEAFP ATKCQIWRG PNRKCNFTS 
      DALMGYANN NRSQGQFYL ITGFKAPFA KTANQQMTE
    CDS sequence
    • >TC005503 RA
      atgaacgtt ttcacgcaa gttttcgga gtggcgctt 
      aattttctc atgattccg ttcaaccct tcggcgccg 
      ccgatgccc tacattgtc agtgagacc acgtgccgg 
      attaccaat ttcaaattt ggtgaaaac gagatgaaa 
      gtgagcctc gcccccaga ggctactgt ccaaactgc 
      tgtccggta aaatccgga gttgacgtc cggttcgtg 
      ctttatacg aaatccact cctgataga ggcgcgatt 
      atcgcacca tcgcccgga gctgcacgc agggccggc 
      gttgacccg ctggcgccc accgtcatc tacatccat 
      gggttctcg gaaccttcg ccggggaaa agcggacgc 
      gggatcgcc catgcttat ctcagccga agggacaat 
      ttcaacgtt attctgctg gactggtcg gatttgtcc 
      acttttccg tggtacctc ccagccgtc cggaacgtg 
      aaaatcgtg gcggaaaaa ttgcggaaa tttttggag 
      gttttcaac gatagcggg gaaattccg ctcgggaat 
      gtgcatttg attgggttc agtctgggc tcgcatatc 
      gccggattt gccggcaaa cagctccgg agggggctc 
      cggattccg aggatcact gccctggat ccggcgttc 
      ccggaatac tcactcaac gacgcctcc cgacgcctc 
      acccgcacc gacgccgac tacatcgac gtgatccac 
      accgacgcg ggcgtcctg ggtctgccc atctcggtg 
      ggtcacgcc gacttttac cccaacggg ggccgagcc 
      ctgcagccc ggctgccag cccagttac ttagtccag 
      ctccggctg gtggatcag atcttcgct tgcagtcat 
      gtgagagcc tggagactg tacgccgag tccgtgatg 
      catccggaa gcgtttccg gcgaccaaa tgccaaatc 
      tggcgcggg cccaaccgg aagtgcaac ttcacgagc 
      gatgcactg atgggttat gccaataac aaccgcagc 
      cagggacag ttttatctg ataacgggg ttcaaagcg 
      ccttttgcc aagactgcc aatcaacaa atgactgaa 
      tag
    mRNA sequence
    • >TC005503 RA
      cgggcaaac gaattacgc gacaattaa cttattaag 
      ttcgaaaat tgtgaaggt cgccgaaga cccaacaat 
      gaagaagtg tttcgcaaa ggctggcct tagccggtt 
      aacattgac atagacatg tgctcctca attgttcaa 
      tgttactgt taacccttc cacagcggt gaagaaccg 
      aaatggcag ctttctaaa tttcatggc aaaagcgtc 
      aacattgaa acggaaggt aatataatg atgatggcg 
      gcgctattt gtggaaacg tcggaggga ctttgacgg 
      gaatgtgtc cgcgaaatt gttaaaaaa tgaacgttt 
      tcacgcaag ttttcggag tggcgctta attttctca 
      tgattccgt tcaaccctt cggcgccgc cgatgccct 
      acattgtca gtgagacca cgtgccgga ttaccaatt 
      tcaaatttg gtgaaaacg agatgaaag tgagcctcg 
      cccccagag gctactgtc caaactgct gtccggtaa 
      aatccggag ttgacgtcc ggttcgtgc tttatacga 
      aatccactc ctgatagag gcgcgatta tcgcaccat 
      cgcccggag ctgcacgca gggccggcg ttgacccgc 
      tggcgccca ccgtcatct acatccatg ggttctcgg 
      aaccttcgc cggggaaaa gcggacgcg ggatcgccc 
      atgcttatc tcagccgaa gggacaatt tcaacgtta 
      ttctgctgg actggtcgg atttgtcca cttttccgt 
      ggtacctcc cagccgtcc ggaacgtga aaatcgtgg 
      cggaaaaat tgcggaaat ttttggagg ttttcaacg 
      atagcgggg aaattccgc tcgggaatg tgcatttga 
      ttgggttca gtctgggct cgcatatcg ccggatttg 
      ccggcaaac agctccgga gggggctcc ggattccga 
      ggatcactg ccctggatc cggcgttcc cggaatact 
      cactcaacg acgcctccc gacgcctca cccgcaccg 
      acgccgact acatcgacg tgatccaca ccgacgcgg 
      gcgtcctgg gtctgccca tctcggtgg gtcacgccg 
      acttttacc ccaacgggg gccgagccc tgcagcccg 
      gctgccagc ccagttact tagtccagc tccggctgg 
      tggatcaga tcttcgctt gcagtcatg tgagagcct 
      ggagactgt acgccgagt ccgtgatgc atccggaag 
      cgtttccgg cgaccaaat gccaaatct ggcgcgggc 
      ccaaccgga agtgcaact tcacgagcg atgcactga 
      tgggttatg ccaataaca accgcagcc agggacagt 
      tttatctga taacggggt tcaaagcgc cttttgcca 
      agactgcca atcaacaaa tgactgaat agtgtgtag 
      gatttttat tgttaattt gttgtacgt tttatttat 
      taaatgaga atgaaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY