General information | |||
TC005500 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12084232..12091989 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC005500 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03968 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
- >TC005500 RA
MNLIPWVLL GLISWTSAQ GELPASEDK PNEAKLMSK ITVGNAPIE QRNYQVAPS PYNLPEQSE PYNIAYEYH NYDQMTKFL RATTSRYPS LTALYSIGK SVQGRDLWV MVVSASPYE HMIGKPDVK YVANMHGNE AVSRELMLH LIHHLVTNY HTDPYIRWL MDNTRIHIM PSMNPDGFE VAKEGACDG GQGRYNARG FDLNRNFPD YFKQNNKRT QPETEAVKE WVSKIQFVL SGSLHGGAL VASYPFDNT PNSLFQSYA SSPSLTPDD DVFKHLALT YSTNHAKMS RGVACRSSQ QGFRRGITN GAEWYPLTG GMQDFNYVW YGCMEVTLE VSCCKYPPA NELPKYWED NRMSLIKFL AEAHRGVHG FVMDENGNP VEKASLKIK TRDVGFQST KYGEFWRIL MPGVYKLEV YADGYVPRE VDVMVVEQH PTLVNVTLH AAKPGQSFK RPVTSPPIT MLSPEAAPS SNVDFSFPQ D
- >TC005500 RB
MNLIPWVLL GLISWTSAQ GELPASEDK PNEAKLMSK ITVGNAPIE QRNYQVAPS PYNLPEQSE PYNIAYEYH NYDQMTKFL RATTSRYPS LTALYSIGK SVQGRDLWV MVVSASPYE HMIGKPDVK YVANMHGNE AVSRELMLH LIHHLVTNY HTDPYIRWL MDNTRIHIM PSMNPDGFE VAKEGACDG GQGRYNARG FDLNRNFPD YFKQNNKRT QPETEAVKE WVSKIQFVL SGSLHGGAL VASYPFDNT PNSLFQSYA SSPSLTPDD DVFKHLALT YSTNHAKMS RGVACRSSQ QGFRRGITN GAEWYPLTG GMQDFNYVW YGCMEVTLE VSCCKYPPA NELPKYWED NRMSLIKFL AEAHRGVHG FVMDENGNP VEKASLKIK TRDVGFQST KYGEFWRIL MPGVYKLEV YADGYVPRE VDVMVVEQH PTLVNVTLH AAKRKDGNF YRPQQQQQP FYHRQPVPH PQQPGFISS ITSGFNNLV NFFG
- >TC005500 RA
atgaatctg ataccatgg gtcctgttg gggttgata tcgtggacg tcagcccaa ggggaactc cccgcttcc gaggacaag ccgaatgag gcgaaacta atgtccaag ataacagtg ggcaatgcg cccatcgag cagaggaat tatcaagtc gcgccctcg ccctacaat ttacccgaa caaagcgag ccctacaat atcgcctac gagtatcat aattacgac caaatgact aagtttctc agagcgaca acgtccaga tatccctcg ttgactgcg ctgtactcg attggaaag tcggtgcaa gggagagat ttgtgggtg atggtagtc tcagcctcc ccttatgaa cacatgatt ggaaaaccc gacgttaag tacgttgca aatatgcac ggaaacgaa gccgtgagc agggaactt atgctacat ctgattcat cacctggtg accaactac cacacggac ccttacatc cgctggctg atggacaac accagaatc cacatcatg ccctccatg aaccccgac ggcttcgag gtggccaaa gagggcgcc tgtgacggt ggtcaaggt cgttacaac gcgcgcggt tttgacttg aaccgcaat ttccccgat tatttcaaa caaaacaat aaacgaacg caaccagaa acagaggcg gtcaaagaa tgggtttcc aaaatccag ttcgttttg tctgggagt ttgcacggt ggcgcttta gtcgccagt tatcccttc gataatact ccgaattca ctgtttcag agctacgcg tcctcaccg tctcttaca ccagacgat gatgttttc aaacattta gcgctgact tactccacg aatcatgcg aagatgagt cgcggagtt gcgtgcagg tcgtcacag cagggattc agaagggga attacaaac ggtgccgaa tggtacccc ctgactgga ggcatgcaa gacttcaac tacgtctgg tacggctgc atggaagtg actctggaa gtgtcctgt tgcaaatat cccccagca aacgaacta ccaaaatac tgggaggac aatcgcatg tccctgatt aaattcctc gccgaagcc catcgtggc gtccacggc ttcgtcatg gacgagaac ggcaatccg gtcgaaaaa gcctctctc aaaataaaa acccgcgat gtgggcttc cagtcgacg aaatacggc gagttctgg agaatcctc atgcccgga gtgtacaaa ctcgaggtt tacgccgac ggttacgtg ccccgagag gtggacgtc atggtggtg gagcaacac cccactctt gtgaacgtg actctgcac gcagccaag cctgggcaa agttttaaa agaccggtc acttctccc ccgatcact atgctctcg ccagaggcg gcaccctct tcaaacgtt gacttttcc ttcccgcaa gactaa
- >TC005500 RB
atgaatctg ataccatgg gtcctgttg gggttgata tcgtggacg tcagcccaa ggggaactc cccgcttcc gaggacaag ccgaatgag gcgaaacta atgtccaag ataacagtg ggcaatgcg cccatcgag cagaggaat tatcaagtc gcgccctcg ccctacaat ttacccgaa caaagcgag ccctacaat atcgcctac gagtatcat aattacgac caaatgact aagtttctc agagcgaca acgtccaga tatccctcg ttgactgcg ctgtactcg attggaaag tcggtgcaa gggagagat ttgtgggtg atggtagtc tcagcctcc ccttatgaa cacatgatt ggaaaaccc gacgttaag tacgttgca aatatgcac ggaaacgaa gccgtgagc agggaactt atgctacat ctgattcat cacctggtg accaactac cacacggac ccttacatc cgctggctg atggacaac accagaatc cacatcatg ccctccatg aaccccgac ggcttcgag gtggccaaa gagggcgcc tgtgacggt ggtcaaggt cgttacaac gcgcgcggt tttgacttg aaccgcaat ttccccgat tatttcaaa caaaacaat aaacgaacg caaccagaa acagaggcg gtcaaagaa tgggtttcc aaaatccag ttcgttttg tctgggagt ttgcacggt ggcgcttta gtcgccagt tatcccttc gataatact ccgaattca ctgtttcag agctacgcg tcctcaccg tctcttaca ccagacgat gatgttttc aaacattta gcgctgact tactccacg aatcatgcg aagatgagt cgcggagtt gcgtgcagg tcgtcacag cagggattc agaagggga attacaaac ggtgccgaa tggtacccc ctgactgga ggcatgcaa gacttcaac tacgtctgg tacggctgc atggaagtg actctggaa gtgtcctgt tgcaaatat cccccagca aacgaacta ccaaaatac tgggaggac aatcgcatg tccctgatt aaattcctc gccgaagcc catcgtggc gtccacggc ttcgtcatg gacgagaac ggcaatccg gtcgaaaaa gcctctctc aaaataaaa acccgcgat gtgggcttc cagtcgacg aaatacggc gagttctgg agaatcctc atgcccgga gtgtacaaa ctcgaggtt tacgccgac ggttacgtg ccccgagag gtggacgtc atggtggtg gagcaacac cccactctt gtgaacgtg actctgcac gcagccaag aggaaggat gggaatttt tatcggccg cagcaacag caacaaccg ttttaccac cggcagccg gtgccgcat ccccagcag ccgggcttc atttcttcc ataactagc gggtttaac aacctggtt aatttcttc ggataa
- >TC005500 RA
aggggactg tccagtgcc tgccttgca ggtgtaagg ctcgttcac tttggttcg gtgtttcgc gttttgtgg tagtgtggc ctggacgga gaaaaatca attggtgga tttatgaca caaggagca aataattat gaatctgat accatgggt cctgttggg gttgatatc gtggacgtc agcccaagg ggaactccc cgcttccga ggacaagcc gaatgaggc gaaactaat gtccaagat aacagtggg caatgcgcc catcgagca gaggaatta tcaagtcgc gccctcgcc ctacaattt acccgaaca aagcgagcc ctacaatat cgcctacga gtatcataa ttacgacca aatgactaa gtttctcag agcgacaac gtccagata tccctcgtt gactgcgct gtactcgat tggaaagtc ggtgcaagg gagagattt gtgggtgat ggtagtctc agcctcccc ttatgaaca catgattgg aaaacccga cgttaagta cgttgcaaa tatgcacgg aaacgaagc cgtgagcag ggaacttat gctacatct gattcatca cctggtgac caactacca cacggaccc ttacatccg ctggctgat ggacaacac cagaatcca catcatgcc ctccatgaa ccccgacgg cttcgaggt ggccaaaga gggcgcctg tgacggtgg tcaaggtcg ttacaacgc gcgcggttt tgacttgaa ccgcaattt ccccgatta tttcaaaca aaacaataa acgaacgca accagaaac agaggcggt caaagaatg ggtttccaa aatccagtt cgttttgtc tgggagttt gcacggtgg cgctttagt cgccagtta tcccttcga taatactcc gaattcact gtttcagag ctacgcgtc ctcaccgtc tcttacacc agacgatga tgttttcaa acatttagc gctgactta ctccacgaa tcatgcgaa gatgagtcg cggagttgc gtgcaggtc gtcacagca gggattcag aaggggaat tacaaacgg tgccgaatg gtaccccct gactggagg catgcaaga cttcaacta cgtctggta cggctgcat ggaagtgac tctggaagt gtcctgttg caaatatcc cccagcaaa cgaactacc aaaatactg ggaggacaa tcgcatgtc cctgattaa attcctcgc cgaagccca tcgtggcgt ccacggctt cgtcatgga cgagaacgg caatccggt cgaaaaagc ctctctcaa aataaaaac ccgcgatgt gggcttcca gtcgacgaa atacggcga gttctggag aatcctcat gcccggagt gtacaaact cgaggttta cgccgacgg ttacgtgcc ccgagaggt ggacgtcat ggtggtgga gcaacaccc cactcttgt gaacgtgac tctgcacgc agccaagcc tgggcaaag ttttaaaag accggtcac ttctccccc gatcactat gctctcgcc agaggcggc accctcttc aaacgttga cttttcctt cccgcaaga ctaacactg tatattttg caccagttg tggtttttt gaccattgt gattttgta gaggaagga tgggaattt ttatcggcc gcagcaaca gcaacaacc gttttacca ccggcagcc ggtgccgca tccccagca gccgggctt catttcttc cataactag cgggtttaa caacctggt taatttctt cggataagt taacgtatt gatacgttc tgcattatt tattttgta tttatgagt aggtttccg aagtctcag gttgctcaa ggagctttt atgttcaaa atcaaacgt tgttgactt ccctgttac agactgtta ataatgtta agaaataaa g
- >TC005500 RB
aggggactg tccagtgcc tgccttgca ggtgtaagg ctcgttcac tttggttcg gtgtttcgc gttttgtgg tagtgtggc ctggacgga gaaaaatca attggtgga tttatgaca caaggagca aataattat gaatctgat accatgggt cctgttggg gttgatatc gtggacgtc agcccaagg ggaactccc cgcttccga ggacaagcc gaatgaggc gaaactaat gtccaagat aacagtggg caatgcgcc catcgagca gaggaatta tcaagtcgc gccctcgcc ctacaattt acccgaaca aagcgagcc ctacaatat cgcctacga gtatcataa ttacgacca aatgactaa gtttctcag agcgacaac gtccagata tccctcgtt gactgcgct gtactcgat tggaaagtc ggtgcaagg gagagattt gtgggtgat ggtagtctc agcctcccc ttatgaaca catgattgg aaaacccga cgttaagta cgttgcaaa tatgcacgg aaacgaagc cgtgagcag ggaacttat gctacatct gattcatca cctggtgac caactacca cacggaccc ttacatccg ctggctgat ggacaacac cagaatcca catcatgcc ctccatgaa ccccgacgg cttcgaggt ggccaaaga gggcgcctg tgacggtgg tcaaggtcg ttacaacgc gcgcggttt tgacttgaa ccgcaattt ccccgatta tttcaaaca aaacaataa acgaacgca accagaaac agaggcggt caaagaatg ggtttccaa aatccagtt cgttttgtc tgggagttt gcacggtgg cgctttagt cgccagtta tcccttcga taatactcc gaattcact gtttcagag ctacgcgtc ctcaccgtc tcttacacc agacgatga tgttttcaa acatttagc gctgactta ctccacgaa tcatgcgaa gatgagtcg cggagttgc gtgcaggtc gtcacagca gggattcag aaggggaat tacaaacgg tgccgaatg gtaccccct gactggagg catgcaaga cttcaacta cgtctggta cggctgcat ggaagtgac tctggaagt gtcctgttg caaatatcc cccagcaaa cgaactacc aaaatactg ggaggacaa tcgcatgtc cctgattaa attcctcgc cgaagccca tcgtggcgt ccacggctt cgtcatgga cgagaacgg caatccggt cgaaaaagc ctctctcaa aataaaaac ccgcgatgt gggcttcca gtcgacgaa atacggcga gttctggag aatcctcat gcccggagt gtacaaact cgaggttta cgccgacgg ttacgtgcc ccgagaggt ggacgtcat ggtggtgga gcaacaccc cactcttgt gaacgtgac tctgcacgc agccaagag gaaggatgg gaattttta tcggccgca gcaacagca acaaccgtt ttaccaccg gcagccggt gccgcatcc ccagcagcc gggcttcat ttcttccat aactagcgg gtttaacaa cctggttaa tttcttcgg ataagttaa cgtattgat acgttctgc attatttat tttgtattt atgagtagg tttccgaag tctcaggtt gctcaagga gcttttatg ttcaaaatc aaacgttgt tgacttccc tgttacaga ctgttaata atgttaaga aataaag
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
