Status
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Gene information
General information
TC034848 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12136511..12138334
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034848
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034848 RA
      MELNNYRNS TNNDRRSVV FLGSPLTDI TANVDREFL 
      RKYNLEPDN AYVVDETRR PIFDEIGET AIQVGGSVT 
      NTVRMFTKL RGFPELVTY LGSVGLDEK GKFVKTELE 
      KEGLGRDLT EIAGGSTGK VAVLVWGTT RTLVTDLGA 
      SRTFSLDED KWAKITRSS FVYFSGFFI SVSFPSLVR 
      IVEQTDKTI CFNLGAPFL CSKYPQEMT YLYKNASLV 
      VGNESEHRA FAKINNLES HDLLEVVMA TNKSFPKSR 
      VVIVTRGGG TVVVVTANG WQEFAVGSL SDEKIVDTS 
      GAGDAFIGG FLAGWVDDA TVADCAKSG ILAARRVIQ 
      TVGFNL
    • >TC034848 RB
      MELNNYRNS TNNDSSRRS VVFLGSPLT DITANVDRE 
      FLRKYNLEP DNAYVVDET RRPIFDEIG ETAIQVGGS 
      VTNTVRMFT KLRGFPELV TYLGSVGLD EKGKFVKTE 
      LEKEGLGRD LTEIAGGST GKVAVLVWG TTRTLVTDL 
      GASRTFSLD EDKWAKITR SSFVYFSGF FISVSFPSL 
      VRIVEQTDK TICFNLGAP FLCSKYPQE MTYLYKNAS 
      LVVGNESEH RAFAKINNL ESHDLLEVV MATNKSFPK 
      SRVVIVTRG GGTVVVVTA NGWQEFAVG SLSDEKIVD 
      TSGAGDAFI GGFLAGWVD DATVADCAK SGILAARRV 
      IQTVGFNL
    CDS sequence
    • >TC034848 RA
      atggaatta aataattat agaaatagt acaaacaac 
      gacagacgg tcggtcgtt ttcctcggg agtccccta 
      actgacatc acggccaac gtcgaccga gaattttta 
      cgaaaatac aacctcgaa cctgataat gcgtacgtc 
      gttgacgaa acgcgtcgc ccgattttt gacgaaatt 
      ggcgaaacg gccattcaa gtcggcggc agtgttacc 
      aacacagtc cggatgttc accaaattg aggggattc 
      cccgaactt gtgacctat ttaggctca gtaggccta 
      gacgaaaag ggcaaattt gtgaaaacc gagctggag 
      aaagagggc ctagggcgc gatttgacc gaaatcgcc 
      gggggaagt accggaaaa gtggcggtt ttggtttgg 
      gggactacc cggactttg gtcaccgac ttgggggct 
      tcccggact ttttcgctc gacgaggac aagtgggcc 
      aaaatcacc cgatcatca tttgtttat ttctccgga 
      ttttttatc agtgtttcc ttcccatcc cttgtccga 
      attgtggaa caaacggac aaaacaatt tgtttcaac 
      ctgggggcg ccttttttg tgctcgaaa tacccccag 
      gaaatgact tatttgtac aaaaatgcg tctctcgtc 
      gttgggaac gaatccgag catcgcgct ttcgcaaag 
      ataaacaac ctggagtca catgatctg ctcgaggtc 
      gttatggca acaaacaag tcgtttccc aagtcacgt 
      gtcgttata gtaacccga gggggcgga accgtggtc 
      gtggtaacc gccaatggc tggcaggaa ttcgccgtt 
      gggtcgcta agcgacgaa aaaatcgtg gacaccagt 
      ggcgccggc gacgcgttc attggcggg ttcctggcc 
      ggttgggtt gacgacgcc actgttgcc gactgtgca 
      aaaagcgga attttggcc gcgaggcgt gttattcaa 
      actgtggga tttaacctt tga
    • >TC034848 RB
      atggaatta aataattat agaaatagt acaaacaac 
      gacagttcc agacggtcg gtcgttttc ctcgggagt 
      cccctaact gacatcacg gccaacgtc gaccgagaa 
      tttttacga aaatacaac ctcgaacct gataatgcg 
      tacgtcgtt gacgaaacg cgtcgcccg atttttgac 
      gaaattggc gaaacggcc attcaagtc ggcggcagt 
      gttaccaac acagtccgg atgttcacc aaattgagg 
      ggattcccc gaacttgtg acctattta ggctcagta 
      ggcctagac gaaaagggc aaatttgtg aaaaccgag 
      ctggagaaa gagggccta gggcgcgat ttgaccgaa 
      atcgccggg ggaagtacc ggaaaagtg gcggttttg 
      gtttggggg actacccgg actttggtc accgacttg 
      ggggcttcc cggactttt tcgctcgac gaggacaag 
      tgggccaaa atcacccga tcatcattt gtttatttc 
      tccggattt tttatcagt gtttccttc ccatccctt 
      gtccgaatt gtggaacaa acggacaaa acaatttgt 
      ttcaacctg ggggcgcct tttttgtgc tcgaaatac 
      ccccaggaa atgacttat ttgtacaaa aatgcgtct 
      ctcgtcgtt gggaacgaa tccgagcat cgcgctttc 
      gcaaagata aacaacctg gagtcacat gatctgctc 
      gaggtcgtt atggcaaca aacaagtcg tttcccaag 
      tcacgtgtc gttatagta acccgaggg ggcggaacc 
      gtggtcgtg gtaaccgcc aatggctgg caggaattc 
      gccgttggg tcgctaagc gacgaaaaa atcgtggac 
      accagtggc gccggcgac gcgttcatt ggcgggttc 
      ctggccggt tgggttgac gacgccact gttgccgac 
      tgtgcaaaa agcggaatt ttggccgcg aggcgtgtt 
      attcaaact gtgggattt aacctttga
    mRNA sequence
    • >TC034848 RA
      cgtgtgttt tttgtgctg cataccata gattacata 
      agtggtgtt tgttagcta agaataact aaatggaat 
      taaataatt atagaaata gtacaaaca acgacagac 
      ggtcggtcg ttttcctcg ggagtcccc taactgaca 
      tcacggcca acgtcgacc gagaatttt tacgaaaat 
      acaacctcg aacctgata atgcgtacg tcgttgacg 
      aaacgcgtc gcccgattt ttgacgaaa ttggcgaaa 
      cggccattc aagtcggcg gcagtgtta ccaacacag 
      tccggatgt tcaccaaat tgaggggat tccccgaac 
      ttgtgacct atttaggct cagtaggcc tagacgaaa 
      agggcaaat ttgtgaaaa ccgagctgg agaaagagg 
      gcctagggc gcgatttga ccgaaatcg ccgggggaa 
      gtaccggaa aagtggcgg ttttggttt gggggacta 
      cccggactt tggtcaccg acttggggg cttcccgga 
      ctttttcgc tcgacgagg acaagtggg ccaaaatca 
      cccgatcat catttgttt atttctccg gatttttta 
      tcagtgttt ccttcccat cccttgtcc gaattgtgg 
      aacaaacgg acaaaacaa tttgtttca acctggggg 
      cgccttttt tgtgctcga aataccccc aggaaatga 
      cttatttgt acaaaaatg cgtctctcg tcgttggga 
      acgaatccg agcatcgcg ctttcgcaa agataaaca 
      acctggagt cacatgatc tgctcgagg tcgttatgg 
      caacaaaca agtcgtttc ccaagtcac gtgtcgtta 
      tagtaaccc gagggggcg gaaccgtgg tcgtggtaa 
      ccgccaatg gctggcagg aattcgccg ttgggtcgc 
      taagcgacg aaaaaatcg tggacacca gtggcgccg 
      gcgacgcgt tcattggcg ggttcctgg ccggttggg 
      ttgacgacg ccactgttg ccgactgtg caaaaagcg 
      gaattttgg ccgcgaggc gtgttattc aaactgtgg 
      gatttaacc tttgacttg aaataaaat tttgtcccg 
      gat
    • >TC034848 RB
      cgtgtgttt tttgtgctg cataccata gattacata 
      agtggtgtt tgttagcta agaataact aaatggaat 
      taaataatt atagaaata gtacaaaca acgacagtt 
      ccagacggt cggtcgttt tcctcggga gtcccctaa 
      ctgacatca cggccaacg tcgaccgag aatttttac 
      gaaaataca acctcgaac ctgataatg cgtacgtcg 
      ttgacgaaa cgcgtcgcc cgatttttg acgaaattg 
      gcgaaacgg ccattcaag tcggcggca gtgttacca 
      acacagtcc ggatgttca ccaaattga ggggattcc 
      ccgaacttg tgacctatt taggctcag taggcctag 
      acgaaaagg gcaaatttg tgaaaaccg agctggaga 
      aagagggcc tagggcgcg atttgaccg aaatcgccg 
      ggggaagta ccggaaaag tggcggttt tggtttggg 
      ggactaccc ggactttgg tcaccgact tgggggctt 
      cccggactt tttcgctcg acgaggaca agtgggcca 
      aaatcaccc gatcatcat ttgtttatt tctccggat 
      tttttatca gtgtttcct tcccatccc ttgtccgaa 
      ttgtggaac aaacggaca aaacaattt gtttcaacc 
      tgggggcgc cttttttgt gctcgaaat acccccagg 
      aaatgactt atttgtaca aaaatgcgt ctctcgtcg 
      ttgggaacg aatccgagc atcgcgctt tcgcaaaga 
      taaacaacc tggagtcac atgatctgc tcgaggtcg 
      ttatggcaa caaacaagt cgtttccca agtcacgtg 
      tcgttatag taacccgag ggggcggaa ccgtggtcg 
      tggtaaccg ccaatggct ggcaggaat tcgccgttg 
      ggtcgctaa gcgacgaaa aaatcgtgg acaccagtg 
      gcgccggcg acgcgttca ttggcgggt tcctggccg 
      gttgggttg acgacgcca ctgttgccg actgtgcaa 
      aaagcggaa ttttggccg cgaggcgtg ttattcaaa 
      ctgtgggat ttaaccttt gacttgaaa taaaatttt 
      gtcccggat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY