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Gene information
General information
TC005491 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12152492..12156119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005491
    iBeetle screen iB_03965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005491 RA
      MEDSKLDKK GYFIPQRYV VALLTMVAL TVVYMMRIS 
      VPVSSRHMV APMNATRAN FSTCPEPSP VVKTSYHIQ 
      DIVFPWTTQ KQLIIKHCF FAGYFIGHF PGGIISDLY 
      GGKHVMSVA ILVSSLITL LTPIVFKAI NGELILVSI 
      TKVLRGISI GMIFPSAHS IISQWCPAR DRGKITGII 
      FAATQWSVT INNVVTEHL IMTMESWSS SYYLYGSMG 
      VVLLLVWLL FASSHPDNC PYLTQKEYN YLKEELGKK 
      SRKKCFIVV LADHVQYGP KPVPWARLL QSLPLWSLV 
      LAQIGHTWI WHGLESDLP KYLRDVLKA DILHRDLMA 
      SIPYAFMGL TTIFMGYVS DYLINRNNS TITTLRKAL 
      TTFGAMGPG IFIVAAGYN GYVSCNRTT AAIIFTFGL 
      CIMSCYYAG TRVNCFDLA PNYSGIVMA LANGFGALP 
      GIVAPEVTA IFTKHASVY GWMFVFWID LGVLAVTNM 
      VFVFFGSAE EQPWNRASG AELDENYSQ ESTEAN
    CDS sequence
    • >TC005491 RA
      atggaagac tcaaagttg gacaaaaaa ggttatttt 
      ataccgcaa cgctacgtt gttgccctt ttgaccatg 
      gtggcactt actgttgtc tatatgatg cgtatttcg 
      gtaccagtg tccagcaga cacatggtc gcacccatg 
      aatgccact cgagcgaat tttagtacc tgtccggag 
      ccatcacca gtcgttaag acttcgtac catatccaa 
      gacatcgtg ttcccctgg accacccaa aagcagctc 
      ataataaaa cattgcttc tttgccggc tatttcatc 
      ggccacttc cctggtggg attatctct gacttgtac 
      ggtggcaag cacgtaatg agtgttgcc atcttggta 
      tcatctctt atcactctc ctcacaccc atcgtgttt 
      aaagcaatc aacggcgag ctgatccta gtctcaatc 
      acaaaagta ttgcgaggg attagcatt gggatgatt 
      ttcccaagc gcccatagc atcataagt caatggtgc 
      ccagcacgc gaccgtggt aaaatcaca ggaattatc 
      ttcgcagct acgcaatgg tccgtcact attaataat 
      gtagtgacc gagcattta atcatgacc atggagagc 
      tggagctcc agctactac ctctacggg tccatgggt 
      gtggtacta ctcctagtg tggctcctt tttgcctcg 
      tcacaccca gacaattgc ccgtacctg acccagaaa 
      gaatacaac tacttgaag gaagaacta ggtaaaaaa 
      tcacgcaaa aagtgtttt attgtggtt ttagccgac 
      catgtccag tacggccct aaaccggtc ccatgggca 
      cgcctcctc caaagccta ccattatgg tcgctggtt 
      ttggcccaa attggccac acgtggatc tggcatggg 
      cttgagagc gacctccct aagtacctt cgggatgtc 
      ctcaaggcc gatattttg caccgggac ctcatggcc 
      tccatcccg tacgcgttc atgggcctg acgaccatt 
      ttcatgggt tacgtctcc gactacttg atcaatcgc 
      aacaactcc acaatcaca acgctacgg aaggcttta 
      acgacgttt ggagccatg ggtccgggc attttcatc 
      gtagctgcg ggttacaac ggttacgtc agttgtaac 
      cggaccaca gctgcgatc attttcacg ttcgggttg 
      tgtataatg agctgttac tacgccggg acacgggtg 
      aattgtttc gatttggcg ccgaattac agtgggatt 
      gttatggcg ctggctaac ggttttggg gctttgcca 
      gggattgtg gctccggaa gttacagca attttcacc 
      aaacacgca agtgtgtat ggttggatg tttgtcttc 
      tggatcgat ttgggtgtg ttggcggtt actaacatg 
      gtttttgtg ttttttggg tcagctgag gaacagcca 
      tggaatcgg gcgagtggt gcagagctc gacgaaaat 
      tacagtcaa gaaagtacg gaagcgaat tga
    mRNA sequence
    • >TC005491 RA
      catgatttg acacgactt gtgacaagt gtgtcaaaa 
      atggaagac tcaaagttg gacaaaaaa ggttatttt 
      ataccgcaa cgctacgtt gttgccctt ttgaccatg 
      gtggcactt actgttgtc tatatgatg cgtatttcg 
      gtaccagtg tccagcaga cacatggtc gcacccatg 
      aatgccact cgagcgaat tttagtacc tgtccggag 
      ccatcacca gtcgttaag acttcgtac catatccaa 
      gacatcgtg ttcccctgg accacccaa aagcagctc 
      ataataaaa cattgcttc tttgccggc tatttcatc 
      ggccacttc cctggtggg attatctct gacttgtac 
      ggtggcaag cacgtaatg agtgttgcc atcttggta 
      tcatctctt atcactctc ctcacaccc atcgtgttt 
      aaagcaatc aacggcgag ctgatccta gtctcaatc 
      acaaaagta ttgcgaggg attagcatt gggatgatt 
      ttcccaagc gcccatagc atcataagt caatggtgc 
      ccagcacgc gaccgtggt aaaatcaca ggaattatc 
      ttcgcagct acgcaatgg tccgtcact attaataat 
      gtagtgacc gagcattta atcatgacc atggagagc 
      tggagctcc agctactac ctctacggg tccatgggt 
      gtggtacta ctcctagtg tggctcctt tttgcctcg 
      tcacaccca gacaattgc ccgtacctg acccagaaa 
      gaatacaac tacttgaag gaagaacta ggtaaaaaa 
      tcacgcaaa aagtgtttt attgtggtt ttagccgac 
      catgtccag tacggccct aaaccggtc ccatgggca 
      cgcctcctc caaagccta ccattatgg tcgctggtt 
      ttggcccaa attggccac acgtggatc tggcatggg 
      cttgagagc gacctccct aagtacctt cgggatgtc 
      ctcaaggcc gatattttg caccgggac ctcatggcc 
      tccatcccg tacgcgttc atgggcctg acgaccatt 
      ttcatgggt tacgtctcc gactacttg atcaatcgc 
      aacaactcc acaatcaca acgctacgg aaggcttta 
      acgacgttt ggagccatg ggtccgggc attttcatc 
      gtagctgcg ggttacaac ggttacgtc agttgtaac 
      cggaccaca gctgcgatc attttcacg ttcgggttg 
      tgtataatg agctgttac tacgccggg acacgggtg 
      aattgtttc gatttggcg ccgaattac agtgggatt 
      gttatggcg ctggctaac ggttttggg gctttgcca 
      gggattgtg gctccggaa gttacagca attttcacc 
      aaacacgca agtgtgtat ggttggatg tttgtcttc 
      tggatcgat ttgggtgtg ttggcggtt actaacatg 
      gtttttgtg ttttttggg tcagctgag gaacagcca 
      tggaatcgg gcgagtggt gcagagctc gacgaaaat 
      tacagtcaa gaaagtacg gaagcgaat tgaaatgtc 
      gtttattaa agacaataa attacaata aatagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY