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Gene information
General information
TC005490 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12159376..12161319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005490
    iBeetle screen iB_03964

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005490 RA
      MTHPCTACL SKFTTCVVI PQRYLLVIM ISLSILNAY 
      TMRVCLSMT ITQMVVKKN VTESTTESV CPSDKTFKG 
      FEMRKETVY DWDEKKQGL ILSSFYWGY FITHIPGGL 
      LAEKLGGKH VLGIGMLTN AVLTILFPF ILKGSEGDW 
      ITVVVLRVV MGLGQGALY PTVAVLLAQ WIPLGERGT 
      ASSISFAGA MMGTVISNS LSGVLMTHY EGWEVVFYF 
      FGVWGLVWN VFWQLVCYS YPRTNPCLK PQEKEFLDQ 
      ELKEVSENK PPTPWKEIL SSLPVWALI VGQFGHDWG 
      WFTMVSDLP KYMDSVLRF HVQSNGLWS AFPYLLMWG 
      ATIGAGYLA DFVINREYL SVANTRRVL STVGTMGPA 
      IFMVAASYV GCNKIAAVM MFTLSLVMM GFFYRGMKV 
      NSIDLSPNF AGTVMALNN GVGVFAGMA APAVVGLLA 
      PNQTMDEWR TVFWVSFGI FAFTNVIYV VFMSGKVQK 
      WNDLKREQV EGFGK
    CDS sequence
    • >TC005490 RA
      atgacgcat ccctgtacc gcctgtttg agcaaattc 
      accacctgt gtcgtaatc ccacagcgc tacttgttg 
      gtaattatg atcagtctc tcaatcctc aacgcgtac 
      acgatgcgt gtttgtctc tccatgacc ataacccag 
      atggtagtc aagaaaaac gtcacggaa agcaccaca 
      gaatcggtc tgtcccagc gataaaacc ttcaaaggc 
      ttcgaaatg cgcaaagaa acggtctat gactgggac 
      gagaagaag caaggtctc attttaagt tcgttttat 
      tggggctac tttataacc catatcccg ggcggcctt 
      ttagccgaa aaactcgga ggcaaacac gtgctagga 
      atagggatg ttaaccaac gccgttctg accattttg 
      ttccctttt attctaaag ggctcggaa ggcgattgg 
      ataactgtg gtggtactt cgggtcgtc atgggtctg 
      ggtcagggg gctttgtac ccaacggtg gccgtgctt 
      ctagcccaa tggatccca cttggggaa cgtggtact 
      gcctcttcc ataagtttc gctggggcg atgatgggt 
      actgttatt tccaactcg ctaagtggg gtactcatg 
      acccattac gagggctgg gaggtggtg ttttacttt 
      ttcggggtt tggggactc gtttggaac gtgttctgg 
      cagttggtt tgctactcg tacccaagg actaaccca 
      tgtttgaag ccccaggag aaggagttc ctggatcag 
      gagttgaag gaggtcagt gagaacaaa cctccaacc 
      ccatggaag gaaattctg agttcgttg cccgtctgg 
      gcgttgatt gtgggccag tttgggcac gactggggt 
      tggtttacc atggttagc gatttgccc aagtacatg 
      gactcggta ctgcgcttc cacgtccag agcaatggg 
      ttatggtcg gcttttccc tacttgttg atgtggggg 
      gcgaccatt ggtgctggg tacctagcc gactttgtt 
      atcaacagg gagtacctt tccgtggcc aacaccaga 
      cgagttctg tcgactgtt ggtactatg ggcccggcc 
      attttcatg gttgcggcg tcctatgtc ggttgtaat 
      aaaatagca gctgtgatg atgttcact ttgtcgttg 
      gttatgatg ggttttttc tatcgtggg atgaaagtg 
      aactcgatt gatttatcg cccaatttt gctggtact 
      gtcatggca ctcaataat ggtgtgggc gttttcgcg 
      ggaatggca gccccggct gtggtgggg cttctggca 
      ccaaatcaa actatggac gaatggagg acggttttt 
      tgggtcagt tttgggatt tttgcattc accaatgtc 
      atatacgtg gtgttcatg tcggggaaa gtccagaaa 
      tggaacgat ttgaagcgg gaacaagtc gagggtttt 
      ggcaaataa
    mRNA sequence
    • >TC005490 RA
      gatctgacc tgacctgac ctgacctaa cagagttta 
      acaaaactt gacaaaaat ttttaatgt tctcgcaaa 
      gttcaagag aaaatttag aataaatag atgtctaga 
      aagtagtta ttgactaga tctgacctg acatgtgat 
      aattttcaa agttgcaaa cttagtatt tgttacttt 
      cgtccagaa atggaacag tttgaacga acaaggaat 
      aatccaact tgtagtcag tcactaaac aataaatta 
      aattttcca aatgtgtca caaacattt ctgacatga 
      cgcatccct gtaccgcct gtttgagca aattcacca 
      cctgtgtcg taatcccac agcgctact tgttggtaa 
      ttatgatca gtctctcaa tcctcaacg cgtacacga 
      tgcgtgttt gtctctcca tgaccataa cccagatgg 
      tagtcaaga aaaacgtca cggaaagca ccacagaat 
      cggtctgtc ccagcgata aaaccttca aaggcttcg 
      aaatgcgca aagaaacgg tctatgact gggacgaga 
      agaagcaag gtctcattt taagttcgt tttattggg 
      gctacttta taacccata tcccgggcg gccttttag 
      ccgaaaaac tcggaggca aacacgtgc taggaatag 
      ggatgttaa ccaacgccg ttctgacca ttttgttcc 
      cttttattc taaagggct cggaaggcg attggataa 
      ctgtggtgg tacttcggg tcgtcatgg gtctgggtc 
      agggggctt tgtacccaa cggtggccg tgcttctag 
      cccaatgga tcccacttg gggaacgtg gtactgcct 
      cttccataa gtttcgctg gggcgatga tgggtactg 
      ttatttcca actcgctaa gtggggtac tcatgaccc 
      attacgagg gctgggagg tggtgtttt actttttcg 
      gggtttggg gactcgttt ggaacgtgt tctggcagt 
      tggtttgct actcgtacc caaggacta acccatgtt 
      tgaagcccc aggagaagg agttcctgg atcaggagt 
      tgaaggagg tcagtgaga acaaacctc caaccccat 
      ggaaggaaa ttctgagtt cgttgcccg tctgggcgt 
      tgattgtgg gccagtttg ggcacgact ggggttggt 
      ttaccatgg ttagcgatt tgcccaagt acatggact 
      cggtactgc gcttccacg tccagagca atgggttat 
      ggtcggctt ttccctact tgttgatgt ggggggcga 
      ccattggtg ctgggtacc tagccgact ttgttatca 
      acagggagt acctttccg tggccaaca ccagacgag 
      ttctgtcga ctgttggta ctatgggcc cggccattt 
      tcatggttg cggcgtcct atgtcggtt gtaataaaa 
      tagcagctg tgatgatgt tcactttgt cgttggtta 
      tgatgggtt ttttctatc gtgggatga aagtgaact 
      cgattgatt tatcgccca attttgctg gtactgtca 
      tggcactca ataatggtg tgggcgttt tcgcgggaa 
      tggcagccc cggctgtgg tggggcttc tggcaccaa 
      atcaaacta tggacgaat ggaggacgg ttttttggg 
      tcagttttg ggatttttg cattcacca atgtcatat 
      acgtggtgt tcatgtcgg ggaaagtcc agaaatgga 
      acgatttga agcgggaac aagtcgagg gttttggca 
      aataaaaaa caattttct tggtgtgat tattttatt 
      ggggaacta taaaactac tagaggggc ttgggtcaa 
      cttcggtac tattccagt cctgttcat cggtcgaaa 
      ctaaagcta tgaaaagta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY