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Gene information
General information
TC005479 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12401731..12451908
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005479
    iBeetle screen iB_03961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005479 RA
      MGVLWRKRS FLLKLIVIV GTAWFTIAF LLYNDDRGS 
      NRIPVPVDD EQDAVPRVL PFRREPPHR EAPKKEEEK 
      PVLLPPASN AGEMGKPVV LPSNLSADV KKLVDAGWQ 
      KNAFNQYVS DMISVHRSL PDPRDEWCK APGRFQEAL 
      PQTSVIICF HNEAWSVLL RTVHSVLDR SPSHLIKEV 
      ILVDDFSDM DHLKQQLVD YFASEPKVK IIRAKKREG 
      LIRARLLGA AHAEGEVLT YLDSHCECT TGWLEPLLD 
      RIARDPTTV VCPVIDVID DTTLEYHFH DSGGVNVGG 
      FDWNLQFNW HAVPEHEKK RHKNPAEPV YSPTMAGGL 
      FSIDKKFFE RLGTYDNGF DIWGGENLE LSFKTWMCG 
      GTLEIVPCS HVGHIFRKR SPYKWRSGV NVLRRNSVR 
      LAEVWLDEY AKYYYQRIG NEKGDFGDI TSRKALREK 
      LGCKSFKWY LDNIYPELF IPGEAVASG EIRNLGIGG 
      KTCLDSPAR RSDLHKPVG LYPCHRQGG NQVNAFAPF 
      NKFLSLQIS VLDRELCID SPCKPEDLH NPIGLWPCH 
      KQGGNQFWM YSKSGEIRR DEACLDYSG QEVILYPCH 
      GSKGNQFWD YNANSKLLR HGSSDKCLA INEAKNKLL 
      MEPCDEEAT RQHWSLENY DASKL
    • >TC005479 RB
      MGVLWRKRS FLLKLIVIV GTAWFTIAF LLYNDDRGS 
      NRIPVPVDD EQDAVPRVL PFRREPPHR EAPKKEEEK 
      PVLLPPASN AGEMGKPVV LPSNLSADV KKLVDAGWQ 
      KNAFNQYVS DMISVHRSL PDPRDEWCK APGRFQEAL 
      PQTSVIICF HNEAWSVLL RTVHSVLDR SPSHLIKEV 
      ILVDDFSDM DHLKQQLVD YFASEPKVK IIRAKKREG 
      LIRARLLGA AHAEGEVLT YLDSHCECT TGWLEPLLD 
      RIARDPTTV VCPVIDVID DTTLEYHFH DSGGVNVGG 
      FDWNLQFNW HAVPEHEKK RHKNPAEPV YSPTMAGGL 
      FSIDKKFFE RLGTYDNGF DIWGGENLE LSFKTWMCG 
      GTLEIVPCS HVGHIFRKR SPYKWRSGV NVLRRNSVR 
      LAEVWLDEY AKYYYQRIG NEKGDFGDI TSRKALREK 
      LGCKSFKWY LDNIYPELF IPGEAVASG EIRNLGIGG 
      KTCLDSPAR RSDLHKPVG LYPCHRQGG NQFWMYSKS 
      GEIRRDEAC LDYSGQEVI LYPCHGSKG NQFWDYNAN 
      SKLLRHGSS DKCLAINEA KNKLLMEPC DEEATRQHW 
      SLENYDASK L
    CDS sequence
    • >TC005479 RA
      atgggcgtg ttgtggagg aaacgttcg ttcttgttg 
      aaactaatc gtgattgtg gggacggcc tggtttacg 
      atagctttt ctcttgtac aatgacgac agaggctcg 
      aatcgcatt ccggtgccg gtggacgat gaacaggac 
      gcagtgccg cgagttttg cccttcagg agggaacct 
      ccacaccgg gaggcgccc aagaaggag gaggagaag 
      ccggtcttg ctaccgcca gcgagcaat gcaggggag 
      atgggcaag ccggtggtg ctgccctcc aatctttca 
      gccgacgta aagaagttg gtcgacgct ggctggcag 
      aaaaacgcc ttcaaccaa tacgtcagc gatatgata 
      agcgtccac cgaagttta ccggatccc agggacgaa 
      tggtgtaag gctccgggt cgtttccag gaagccctc 
      ccccaaacc tccgtcatt atttgtttc cacaatgag 
      gcctggtct gtcctcctt cgaacagtt cattcagtc 
      ctcgaccgc tccccttca catctcatc aaggaggtg 
      atccttgtg gacgacttc tccgacatg gatcatctc 
      aagcagcaa ctagtcgat tatttcgcc tccgaaccc 
      aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt 
      ttgattaga gcccgtctt ctgggggcg gcacatgcc 
      gaaggcgaa gttttgact tatctggac tcgcactgc 
      gagtgcacg accggctgg ttggagccg cttttggat 
      cggatagca agggatccg acgacggtg gtgtgtccg 
      gttatagac gtaatcgat gatacaaca ttggagtat 
      catttccat gatagtgga ggagtcaat gtgggaggg 
      ttcgactgg aatttacag ttcaattgg cacgcggtg 
      cccgaacac gagaagaag aggcacaag aatccggcc 
      gaaccggtg tatagtccc accatggct gggggactt 
      tttagcatt gataagaaa tttttcgaa cgtctggga 
      acctacgac aacggcttc gacatttgg ggcggcgag 
      aacttggag ctctccttc aagacgtgg atgtgcggt 
      ggcactctc gaaatcgtt ccgtgctca cacgtcggt 
      cacatcttc cgaaaacgt tcgccgtat aaatggcga 
      agtggcgtg aacgtttta cgccgaaat tctgtccga 
      ttggccgaa gtctggctg gacgaatac gccaagtat 
      tactaccag cggataggc aacgaaaag ggcgatttc 
      ggagacatc acctcgagg aaagcactc cgcgaaaag 
      ctgggctgt aagagcttc aagtggtat ttggacaac 
      atctatccg gagttgttt attccgggc gaagcggtc 
      gccagtgga gagattcgc aacttgggc attggcgga 
      aagacctgt ttggattct ccagctcgt cgcagcgac 
      ttacacaaa cctgtgggc ttgtaccct tgccaccgc 
      caaggcgga aatcaggta aatgcgttt gcaccattc 
      aacaaattt ctttcttta cagatttcg gtcttggat 
      cgcgagctc tgcatcgat tcgccttgt aaacccgag 
      gacttgcac aatccgatc gggctctgg ccctgccac 
      aagcagggc gggaatcag ttctggatg tacagtaaa 
      agtggtgag atccgtcga gacgaggcc tgccttgac 
      tacagcggt caagaggtg attctgtac ccgtgtcac 
      ggcagcaag ggcaaccaa ttttgggat tacaacgcc 
      aacagcaaa cttttaagg catggaagt agcgataaa 
      tgtttggcc ataaacgaa gcgaaaaat aaactacta 
      atggagccc tgcgacgaa gaggccaca aggcaacac 
      tggtcgttg gagaactac gacgccagc aaattgtga
    • >TC005479 RB
      atgggcgtg ttgtggagg aaacgttcg ttcttgttg 
      aaactaatc gtgattgtg gggacggcc tggtttacg 
      atagctttt ctcttgtac aatgacgac agaggctcg 
      aatcgcatt ccggtgccg gtggacgat gaacaggac 
      gcagtgccg cgagttttg cccttcagg agggaacct 
      ccacaccgg gaggcgccc aagaaggag gaggagaag 
      ccggtcttg ctaccgcca gcgagcaat gcaggggag 
      atgggcaag ccggtggtg ctgccctcc aatctttca 
      gccgacgta aagaagttg gtcgacgct ggctggcag 
      aaaaacgcc ttcaaccaa tacgtcagc gatatgata 
      agcgtccac cgaagttta ccggatccc agggacgaa 
      tggtgtaag gctccgggt cgtttccag gaagccctc 
      ccccaaacc tccgtcatt atttgtttc cacaatgag 
      gcctggtct gtcctcctt cgaacagtt cattcagtc 
      ctcgaccgc tccccttca catctcatc aaggaggtg 
      atccttgtg gacgacttc tccgacatg gatcatctc 
      aagcagcaa ctagtcgat tatttcgcc tccgaaccc 
      aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt 
      ttgattaga gcccgtctt ctgggggcg gcacatgcc 
      gaaggcgaa gttttgact tatctggac tcgcactgc 
      gagtgcacg accggctgg ttggagccg cttttggat 
      cggatagca agggatccg acgacggtg gtgtgtccg 
      gttatagac gtaatcgat gatacaaca ttggagtat 
      catttccat gatagtgga ggagtcaat gtgggaggg 
      ttcgactgg aatttacag ttcaattgg cacgcggtg 
      cccgaacac gagaagaag aggcacaag aatccggcc 
      gaaccggtg tatagtccc accatggct gggggactt 
      tttagcatt gataagaaa tttttcgaa cgtctggga 
      acctacgac aacggcttc gacatttgg ggcggcgag 
      aacttggag ctctccttc aagacgtgg atgtgcggt 
      ggcactctc gaaatcgtt ccgtgctca cacgtcggt 
      cacatcttc cgaaaacgt tcgccgtat aaatggcga 
      agtggcgtg aacgtttta cgccgaaat tctgtccga 
      ttggccgaa gtctggctg gacgaatac gccaagtat 
      tactaccag cggataggc aacgaaaag ggcgatttc 
      ggagacatc acctcgagg aaagcactc cgcgaaaag 
      ctgggctgt aagagcttc aagtggtat ttggacaac 
      atctatccg gagttgttt attccgggc gaagcggtc 
      gccagtgga gagattcgc aacttgggc attggcgga 
      aagacctgt ttggattct ccagctcgt cgcagcgac 
      ttacacaaa cctgtgggc ttgtaccct tgccaccgc 
      caaggcgga aatcagttc tggatgtac agtaaaagt 
      ggtgagatc cgtcgagac gaggcctgc cttgactac 
      agcggtcaa gaggtgatt ctgtacccg tgtcacggc 
      agcaagggc aaccaattt tgggattac aacgccaac 
      agcaaactt ttaaggcat ggaagtagc gataaatgt 
      ttggccata aacgaagcg aaaaataaa ctactaatg 
      gagccctgc gacgaagag gccacaagg caacactgg 
      tcgttggag aactacgac gccagcaaa ttgtga
    mRNA sequence
    • >TC005479 RA
      tgcgttctt gtttcgcgt tgcttaaca tttatcgcg 
      tcttgtgct agattactc catccatcc acttactca 
      tcgccagtg tttcttcct ttttttgct cgcactcga 
      tcgactttc gagaggaaa tcgatgggc gaacgtgac 
      gcgaacaat taccgtaaa taatgcaat tattcgcga 
      atttacgac caaatctaa cttttgcga tgatctctt 
      caagacgtt tcaatcaag gacggatgt agcgaagcg 
      gcaattaaa tttgcgttt tattaagaa aatgggaat 
      ttcgccagt tatttatta atgtgtgag tttgaattc 
      tttccacac tttctcaat tactttctt cgaaaaaat 
      tactacatt atgctcatt cgagttgtg aaagatttt 
      tacgcgaag ttcggaaac tttgtaagc attttcgta 
      attaaatcg accgttttg tcatcacag tgtttattt 
      tgtttccag attgtgtgg ctactattc ggacctcaa 
      ctttaagag atgatggag tctctcaac ttcacacat 
      ctcgccgtt tgcacgctc tcatccccc tttgacaaa 
      cctcacctc ttgtgcacc tttctgtga taaaccgtt 
      atcatagtg ttatcatgt caaaatgct aatccgaaa 
      gtttagata agaagataa agacgtgga atagtgtgt 
      gtgcgcgtg tgtaaatcc tcgaaatat tcgtgtaca 
      agcctgacg atgggcgtg ttgtggagg aaacgttcg 
      ttcttgttg aaactaatc gtgattgtg gggacggcc 
      tggtttacg atagctttt ctcttgtac aatgacgac 
      agaggctcg aatcgcatt ccggtgccg gtggacgat 
      gaacaggac gcagtgccg cgagttttg cccttcagg 
      agggaacct ccacaccgg gaggcgccc aagaaggag 
      gaggagaag ccggtcttg ctaccgcca gcgagcaat 
      gcaggggag atgggcaag ccggtggtg ctgccctcc 
      aatctttca gccgacgta aagaagttg gtcgacgct 
      ggctggcag aaaaacgcc ttcaaccaa tacgtcagc 
      gatatgata agcgtccac cgaagttta ccggatccc 
      agggacgaa tggtgtaag gctccgggt cgtttccag 
      gaagccctc ccccaaacc tccgtcatt atttgtttc 
      cacaatgag gcctggtct gtcctcctt cgaacagtt 
      cattcagtc ctcgaccgc tccccttca catctcatc 
      aaggaggtg atccttgtg gacgacttc tccgacatg 
      gatcatctc aagcagcaa ctagtcgat tatttcgcc 
      tccgaaccc aaagtcaag ataatccgt gcgaaaaaa 
      cgcgaaggt ttgattaga gcccgtctt ctgggggcg 
      gcacatgcc gaaggcgaa gttttgact tatctggac 
      tcgcactgc gagtgcacg accggctgg ttggagccg 
      cttttggat cggatagca agggatccg acgacggtg 
      gtgtgtccg gttatagac gtaatcgat gatacaaca 
      ttggagtat catttccat gatagtgga ggagtcaat 
      gtgggaggg ttcgactgg aatttacag ttcaattgg 
      cacgcggtg cccgaacac gagaagaag aggcacaag 
      aatccggcc gaaccggtg tatagtccc accatggct 
      gggggactt tttagcatt gataagaaa tttttcgaa 
      cgtctggga acctacgac aacggcttc gacatttgg 
      ggcggcgag aacttggag ctctccttc aagacgtgg 
      atgtgcggt ggcactctc gaaatcgtt ccgtgctca 
      cacgtcggt cacatcttc cgaaaacgt tcgccgtat 
      aaatggcga agtggcgtg aacgtttta cgccgaaat 
      tctgtccga ttggccgaa gtctggctg gacgaatac 
      gccaagtat tactaccag cggataggc aacgaaaag 
      ggcgatttc ggagacatc acctcgagg aaagcactc 
      cgcgaaaag ctgggctgt aagagcttc aagtggtat 
      ttggacaac atctatccg gagttgttt attccgggc 
      gaagcggtc gccagtgga gagattcgc aacttgggc 
      attggcgga aagacctgt ttggattct ccagctcgt 
      cgcagcgac ttacacaaa cctgtgggc ttgtaccct 
      tgccaccgc caaggcgga aatcaggta aatgcgttt 
      gcaccattc aacaaattt ctttcttta cagatttcg 
      gtcttggat cgcgagctc tgcatcgat tcgccttgt 
      aaacccgag gacttgcac aatccgatc gggctctgg 
      ccctgccac aagcagggc gggaatcag ttctggatg 
      tacagtaaa agtggtgag atccgtcga gacgaggcc 
      tgccttgac tacagcggt caagaggtg attctgtac 
      ccgtgtcac ggcagcaag ggcaaccaa ttttgggat 
      tacaacgcc aacagcaaa cttttaagg catggaagt 
      agcgataaa tgtttggcc ataaacgaa gcgaaaaat 
      aaactacta atggagccc tgcgacgaa gaggccaca 
      aggcaacac tggtcgttg gagaactac gacgccagc 
      aaattgtga ttgttttag tttatgtgt gtgtggcaa 
      aaagacgag taaagtaag tgtacaaaa gttttgtat 
      aaatgatcg gttttgcag ttttataac gaattgtag 
      attctagca acgactgtg tctatcaat atctacata 
      tccaattgt tttagcgct caattgtta cattccttt 
      acttaagac tgtaaataa ctggttaca tatccaaca 
      aatatcatt taagcaagc ggcctgtgt atataataa 
      caattgaaa agtacttaa tttcatggc aaattattt 
      tcgaaaacg atcatgtga taattagtc ctttaatta 
      tgtaaataa ttctattat ttgctcaat ttcgttccc 
      ttttacgtc aattattct tcaaagggc cataaagca 
      gcgttatgt aaataactc gcagtattt tatcctgat 
      tttattatt acataatca taatgtact taatatagt 
      gtactagac atgtaaatg taggccagt gtatgtatt 
      tttataacg tgccttatt tgtgtatat aaaacgtta 
      ataaaatta tgttgaaat c
    • >TC005479 RB
      tgcgttctt gtttcgcgt tgcttaaca tttatcgcg 
      tcttgtgct agattactc catccatcc acttactca 
      tcgccagtg tttcttcct ttttttgct cgcactcga 
      tcgactttc gagaggaaa tcgatgggc gaacgtgac 
      gcgaacaat taccgtaaa taatgcaat tattcgcga 
      atttacgac caaatctaa cttttgcga tgatctctt 
      caagacgtt tcaatcaag gacggatgt agcgaagcg 
      gcaattaaa tttgcgttt tattaagaa aatgggaat 
      ttcgccagt tatttatta atgtgtgag tttgaattc 
      tttccacac tttctcaat tactttctt cgaaaaaat 
      tactacatt atgctcatt cgagttgtg aaagatttt 
      tacgcgaag ttcggaaac tttgtaagc attttcgta 
      attaaatcg accgttttg tcatcacag tgtttattt 
      tgtttccag attgtgtgg ctactattc ggacctcaa 
      ctttaagag atgatggag tctctcaac ttcacacat 
      ctcgccgtt tgcacgctc tcatccccc tttgacaaa 
      cctcacctc ttgtgcacc tttctgtga taaaccgtt 
      atcatagtg ttatcatgt caaaatgct aatccgaaa 
      gtttagata agaagataa agacgtgga atagtgtgt 
      gtgcgcgtg tgtaaatcc tcgaaatat tcgtgtaca 
      agcctgacg atgggcgtg ttgtggagg aaacgttcg 
      ttcttgttg aaactaatc gtgattgtg gggacggcc 
      tggtttacg atagctttt ctcttgtac aatgacgac 
      agaggctcg aatcgcatt ccggtgccg gtggacgat 
      gaacaggac gcagtgccg cgagttttg cccttcagg 
      agggaacct ccacaccgg gaggcgccc aagaaggag 
      gaggagaag ccggtcttg ctaccgcca gcgagcaat 
      gcaggggag atgggcaag ccggtggtg ctgccctcc 
      aatctttca gccgacgta aagaagttg gtcgacgct 
      ggctggcag aaaaacgcc ttcaaccaa tacgtcagc 
      gatatgata agcgtccac cgaagttta ccggatccc 
      agggacgaa tggtgtaag gctccgggt cgtttccag 
      gaagccctc ccccaaacc tccgtcatt atttgtttc 
      cacaatgag gcctggtct gtcctcctt cgaacagtt 
      cattcagtc ctcgaccgc tccccttca catctcatc 
      aaggaggtg atccttgtg gacgacttc tccgacatg 
      gatcatctc aagcagcaa ctagtcgat tatttcgcc 
      tccgaaccc aaagtcaag ataatccgt gcgaaaaaa 
      cgcgaaggt ttgattaga gcccgtctt ctgggggcg 
      gcacatgcc gaaggcgaa gttttgact tatctggac 
      tcgcactgc gagtgcacg accggctgg ttggagccg 
      cttttggat cggatagca agggatccg acgacggtg 
      gtgtgtccg gttatagac gtaatcgat gatacaaca 
      ttggagtat catttccat gatagtgga ggagtcaat 
      gtgggaggg ttcgactgg aatttacag ttcaattgg 
      cacgcggtg cccgaacac gagaagaag aggcacaag 
      aatccggcc gaaccggtg tatagtccc accatggct 
      gggggactt tttagcatt gataagaaa tttttcgaa 
      cgtctggga acctacgac aacggcttc gacatttgg 
      ggcggcgag aacttggag ctctccttc aagacgtgg 
      atgtgcggt ggcactctc gaaatcgtt ccgtgctca 
      cacgtcggt cacatcttc cgaaaacgt tcgccgtat 
      aaatggcga agtggcgtg aacgtttta cgccgaaat 
      tctgtccga ttggccgaa gtctggctg gacgaatac 
      gccaagtat tactaccag cggataggc aacgaaaag 
      ggcgatttc ggagacatc acctcgagg aaagcactc 
      cgcgaaaag ctgggctgt aagagcttc aagtggtat 
      ttggacaac atctatccg gagttgttt attccgggc 
      gaagcggtc gccagtgga gagattcgc aacttgggc 
      attggcgga aagacctgt ttggattct ccagctcgt 
      cgcagcgac ttacacaaa cctgtgggc ttgtaccct 
      tgccaccgc caaggcgga aatcagttc tggatgtac 
      agtaaaagt ggtgagatc cgtcgagac gaggcctgc 
      cttgactac agcggtcaa gaggtgatt ctgtacccg 
      tgtcacggc agcaagggc aaccaattt tgggattac 
      aacgccaac agcaaactt ttaaggcat ggaagtagc 
      gataaatgt ttggccata aacgaagcg aaaaataaa 
      ctactaatg gagccctgc gacgaagag gccacaagg 
      caacactgg tcgttggag aactacgac gccagcaaa 
      ttgtgattg ttttagttt atgtgtgtg tggcaaaaa 
      gacgagtaa agtaagtgt acaaaagtt ttgtataaa 
      tgatcggtt ttgcagttt tataacgaa ttgtagatt 
      ctagcaacg actgtgtct atcaatatc tacatatcc 
      aattgtttt agcgctcaa ttgttacat tcctttact 
      taagactgt aaataactg gttacatat ccaacaaat 
      atcatttaa gcaagcggc ctgtgtata taataacaa 
      ttgaaaagt acttaattt catggcaaa ttattttcg 
      aaaacgatc atgtgataa ttagtcctt taattatgt 
      aaataattc tattatttg ctcaatttc gttcccttt 
      tacgtcaat tattcttca aagggccat aaagcagcg 
      ttatgtaaa taactcgca gtattttat cctgatttt 
      attattaca taatcataa tgtacttaa tatagtgta 
      ctagacatg taaatgtag gccagtgta tgtattttt 
      ataacgtgc cttatttgt gtatataaa acgttaata 
      aaattatgt tgaaatc
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