| General information | |||
| TC005479 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12401731..12451908 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005479 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03961 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005479 RA
MGVLWRKRS FLLKLIVIV GTAWFTIAF LLYNDDRGS NRIPVPVDD EQDAVPRVL PFRREPPHR EAPKKEEEK PVLLPPASN AGEMGKPVV LPSNLSADV KKLVDAGWQ KNAFNQYVS DMISVHRSL PDPRDEWCK APGRFQEAL PQTSVIICF HNEAWSVLL RTVHSVLDR SPSHLIKEV ILVDDFSDM DHLKQQLVD YFASEPKVK IIRAKKREG LIRARLLGA AHAEGEVLT YLDSHCECT TGWLEPLLD RIARDPTTV VCPVIDVID DTTLEYHFH DSGGVNVGG FDWNLQFNW HAVPEHEKK RHKNPAEPV YSPTMAGGL FSIDKKFFE RLGTYDNGF DIWGGENLE LSFKTWMCG GTLEIVPCS HVGHIFRKR SPYKWRSGV NVLRRNSVR LAEVWLDEY AKYYYQRIG NEKGDFGDI TSRKALREK LGCKSFKWY LDNIYPELF IPGEAVASG EIRNLGIGG KTCLDSPAR RSDLHKPVG LYPCHRQGG NQVNAFAPF NKFLSLQIS VLDRELCID SPCKPEDLH NPIGLWPCH KQGGNQFWM YSKSGEIRR DEACLDYSG QEVILYPCH GSKGNQFWD YNANSKLLR HGSSDKCLA INEAKNKLL MEPCDEEAT RQHWSLENY DASKL
- >TC005479 RB
MGVLWRKRS FLLKLIVIV GTAWFTIAF LLYNDDRGS NRIPVPVDD EQDAVPRVL PFRREPPHR EAPKKEEEK PVLLPPASN AGEMGKPVV LPSNLSADV KKLVDAGWQ KNAFNQYVS DMISVHRSL PDPRDEWCK APGRFQEAL PQTSVIICF HNEAWSVLL RTVHSVLDR SPSHLIKEV ILVDDFSDM DHLKQQLVD YFASEPKVK IIRAKKREG LIRARLLGA AHAEGEVLT YLDSHCECT TGWLEPLLD RIARDPTTV VCPVIDVID DTTLEYHFH DSGGVNVGG FDWNLQFNW HAVPEHEKK RHKNPAEPV YSPTMAGGL FSIDKKFFE RLGTYDNGF DIWGGENLE LSFKTWMCG GTLEIVPCS HVGHIFRKR SPYKWRSGV NVLRRNSVR LAEVWLDEY AKYYYQRIG NEKGDFGDI TSRKALREK LGCKSFKWY LDNIYPELF IPGEAVASG EIRNLGIGG KTCLDSPAR RSDLHKPVG LYPCHRQGG NQFWMYSKS GEIRRDEAC LDYSGQEVI LYPCHGSKG NQFWDYNAN SKLLRHGSS DKCLAINEA KNKLLMEPC DEEATRQHW SLENYDASK L
- >TC005479 RA
atgggcgtg ttgtggagg aaacgttcg ttcttgttg aaactaatc gtgattgtg gggacggcc tggtttacg atagctttt ctcttgtac aatgacgac agaggctcg aatcgcatt ccggtgccg gtggacgat gaacaggac gcagtgccg cgagttttg cccttcagg agggaacct ccacaccgg gaggcgccc aagaaggag gaggagaag ccggtcttg ctaccgcca gcgagcaat gcaggggag atgggcaag ccggtggtg ctgccctcc aatctttca gccgacgta aagaagttg gtcgacgct ggctggcag aaaaacgcc ttcaaccaa tacgtcagc gatatgata agcgtccac cgaagttta ccggatccc agggacgaa tggtgtaag gctccgggt cgtttccag gaagccctc ccccaaacc tccgtcatt atttgtttc cacaatgag gcctggtct gtcctcctt cgaacagtt cattcagtc ctcgaccgc tccccttca catctcatc aaggaggtg atccttgtg gacgacttc tccgacatg gatcatctc aagcagcaa ctagtcgat tatttcgcc tccgaaccc aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt ttgattaga gcccgtctt ctgggggcg gcacatgcc gaaggcgaa gttttgact tatctggac tcgcactgc gagtgcacg accggctgg ttggagccg cttttggat cggatagca agggatccg acgacggtg gtgtgtccg gttatagac gtaatcgat gatacaaca ttggagtat catttccat gatagtgga ggagtcaat gtgggaggg ttcgactgg aatttacag ttcaattgg cacgcggtg cccgaacac gagaagaag aggcacaag aatccggcc gaaccggtg tatagtccc accatggct gggggactt tttagcatt gataagaaa tttttcgaa cgtctggga acctacgac aacggcttc gacatttgg ggcggcgag aacttggag ctctccttc aagacgtgg atgtgcggt ggcactctc gaaatcgtt ccgtgctca cacgtcggt cacatcttc cgaaaacgt tcgccgtat aaatggcga agtggcgtg aacgtttta cgccgaaat tctgtccga ttggccgaa gtctggctg gacgaatac gccaagtat tactaccag cggataggc aacgaaaag ggcgatttc ggagacatc acctcgagg aaagcactc cgcgaaaag ctgggctgt aagagcttc aagtggtat ttggacaac atctatccg gagttgttt attccgggc gaagcggtc gccagtgga gagattcgc aacttgggc attggcgga aagacctgt ttggattct ccagctcgt cgcagcgac ttacacaaa cctgtgggc ttgtaccct tgccaccgc caaggcgga aatcaggta aatgcgttt gcaccattc aacaaattt ctttcttta cagatttcg gtcttggat cgcgagctc tgcatcgat tcgccttgt aaacccgag gacttgcac aatccgatc gggctctgg ccctgccac aagcagggc gggaatcag ttctggatg tacagtaaa agtggtgag atccgtcga gacgaggcc tgccttgac tacagcggt caagaggtg attctgtac ccgtgtcac ggcagcaag ggcaaccaa ttttgggat tacaacgcc aacagcaaa cttttaagg catggaagt agcgataaa tgtttggcc ataaacgaa gcgaaaaat aaactacta atggagccc tgcgacgaa gaggccaca aggcaacac tggtcgttg gagaactac gacgccagc aaattgtga
- >TC005479 RB
atgggcgtg ttgtggagg aaacgttcg ttcttgttg aaactaatc gtgattgtg gggacggcc tggtttacg atagctttt ctcttgtac aatgacgac agaggctcg aatcgcatt ccggtgccg gtggacgat gaacaggac gcagtgccg cgagttttg cccttcagg agggaacct ccacaccgg gaggcgccc aagaaggag gaggagaag ccggtcttg ctaccgcca gcgagcaat gcaggggag atgggcaag ccggtggtg ctgccctcc aatctttca gccgacgta aagaagttg gtcgacgct ggctggcag aaaaacgcc ttcaaccaa tacgtcagc gatatgata agcgtccac cgaagttta ccggatccc agggacgaa tggtgtaag gctccgggt cgtttccag gaagccctc ccccaaacc tccgtcatt atttgtttc cacaatgag gcctggtct gtcctcctt cgaacagtt cattcagtc ctcgaccgc tccccttca catctcatc aaggaggtg atccttgtg gacgacttc tccgacatg gatcatctc aagcagcaa ctagtcgat tatttcgcc tccgaaccc aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt ttgattaga gcccgtctt ctgggggcg gcacatgcc gaaggcgaa gttttgact tatctggac tcgcactgc gagtgcacg accggctgg ttggagccg cttttggat cggatagca agggatccg acgacggtg gtgtgtccg gttatagac gtaatcgat gatacaaca ttggagtat catttccat gatagtgga ggagtcaat gtgggaggg ttcgactgg aatttacag ttcaattgg cacgcggtg cccgaacac gagaagaag aggcacaag aatccggcc gaaccggtg tatagtccc accatggct gggggactt tttagcatt gataagaaa tttttcgaa cgtctggga acctacgac aacggcttc gacatttgg ggcggcgag aacttggag ctctccttc aagacgtgg atgtgcggt ggcactctc gaaatcgtt ccgtgctca cacgtcggt cacatcttc cgaaaacgt tcgccgtat aaatggcga agtggcgtg aacgtttta cgccgaaat tctgtccga ttggccgaa gtctggctg gacgaatac gccaagtat tactaccag cggataggc aacgaaaag ggcgatttc ggagacatc acctcgagg aaagcactc cgcgaaaag ctgggctgt aagagcttc aagtggtat ttggacaac atctatccg gagttgttt attccgggc gaagcggtc gccagtgga gagattcgc aacttgggc attggcgga aagacctgt ttggattct ccagctcgt cgcagcgac ttacacaaa cctgtgggc ttgtaccct tgccaccgc caaggcgga aatcagttc tggatgtac agtaaaagt ggtgagatc cgtcgagac gaggcctgc cttgactac agcggtcaa gaggtgatt ctgtacccg tgtcacggc agcaagggc aaccaattt tgggattac aacgccaac agcaaactt ttaaggcat ggaagtagc gataaatgt ttggccata aacgaagcg aaaaataaa ctactaatg gagccctgc gacgaagag gccacaagg caacactgg tcgttggag aactacgac gccagcaaa ttgtga
- >TC005479 RA
tgcgttctt gtttcgcgt tgcttaaca tttatcgcg tcttgtgct agattactc catccatcc acttactca tcgccagtg tttcttcct ttttttgct cgcactcga tcgactttc gagaggaaa tcgatgggc gaacgtgac gcgaacaat taccgtaaa taatgcaat tattcgcga atttacgac caaatctaa cttttgcga tgatctctt caagacgtt tcaatcaag gacggatgt agcgaagcg gcaattaaa tttgcgttt tattaagaa aatgggaat ttcgccagt tatttatta atgtgtgag tttgaattc tttccacac tttctcaat tactttctt cgaaaaaat tactacatt atgctcatt cgagttgtg aaagatttt tacgcgaag ttcggaaac tttgtaagc attttcgta attaaatcg accgttttg tcatcacag tgtttattt tgtttccag attgtgtgg ctactattc ggacctcaa ctttaagag atgatggag tctctcaac ttcacacat ctcgccgtt tgcacgctc tcatccccc tttgacaaa cctcacctc ttgtgcacc tttctgtga taaaccgtt atcatagtg ttatcatgt caaaatgct aatccgaaa gtttagata agaagataa agacgtgga atagtgtgt gtgcgcgtg tgtaaatcc tcgaaatat tcgtgtaca agcctgacg atgggcgtg ttgtggagg aaacgttcg ttcttgttg aaactaatc gtgattgtg gggacggcc tggtttacg atagctttt ctcttgtac aatgacgac agaggctcg aatcgcatt ccggtgccg gtggacgat gaacaggac gcagtgccg cgagttttg cccttcagg agggaacct ccacaccgg gaggcgccc aagaaggag gaggagaag ccggtcttg ctaccgcca gcgagcaat gcaggggag atgggcaag ccggtggtg ctgccctcc aatctttca gccgacgta aagaagttg gtcgacgct ggctggcag aaaaacgcc ttcaaccaa tacgtcagc gatatgata agcgtccac cgaagttta ccggatccc agggacgaa tggtgtaag gctccgggt cgtttccag gaagccctc ccccaaacc tccgtcatt atttgtttc cacaatgag gcctggtct gtcctcctt cgaacagtt cattcagtc ctcgaccgc tccccttca catctcatc aaggaggtg atccttgtg gacgacttc tccgacatg gatcatctc aagcagcaa ctagtcgat tatttcgcc tccgaaccc aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt ttgattaga gcccgtctt ctgggggcg gcacatgcc gaaggcgaa gttttgact tatctggac tcgcactgc gagtgcacg accggctgg ttggagccg cttttggat cggatagca agggatccg acgacggtg gtgtgtccg gttatagac gtaatcgat gatacaaca ttggagtat catttccat gatagtgga ggagtcaat gtgggaggg ttcgactgg aatttacag ttcaattgg cacgcggtg cccgaacac gagaagaag aggcacaag aatccggcc gaaccggtg tatagtccc accatggct gggggactt tttagcatt gataagaaa tttttcgaa cgtctggga acctacgac aacggcttc gacatttgg ggcggcgag aacttggag ctctccttc aagacgtgg atgtgcggt ggcactctc gaaatcgtt ccgtgctca cacgtcggt cacatcttc cgaaaacgt tcgccgtat aaatggcga agtggcgtg aacgtttta cgccgaaat tctgtccga ttggccgaa gtctggctg gacgaatac gccaagtat tactaccag cggataggc aacgaaaag ggcgatttc ggagacatc acctcgagg aaagcactc cgcgaaaag ctgggctgt aagagcttc aagtggtat ttggacaac atctatccg gagttgttt attccgggc gaagcggtc gccagtgga gagattcgc aacttgggc attggcgga aagacctgt ttggattct ccagctcgt cgcagcgac ttacacaaa cctgtgggc ttgtaccct tgccaccgc caaggcgga aatcaggta aatgcgttt gcaccattc aacaaattt ctttcttta cagatttcg gtcttggat cgcgagctc tgcatcgat tcgccttgt aaacccgag gacttgcac aatccgatc gggctctgg ccctgccac aagcagggc gggaatcag ttctggatg tacagtaaa agtggtgag atccgtcga gacgaggcc tgccttgac tacagcggt caagaggtg attctgtac ccgtgtcac ggcagcaag ggcaaccaa ttttgggat tacaacgcc aacagcaaa cttttaagg catggaagt agcgataaa tgtttggcc ataaacgaa gcgaaaaat aaactacta atggagccc tgcgacgaa gaggccaca aggcaacac tggtcgttg gagaactac gacgccagc aaattgtga ttgttttag tttatgtgt gtgtggcaa aaagacgag taaagtaag tgtacaaaa gttttgtat aaatgatcg gttttgcag ttttataac gaattgtag attctagca acgactgtg tctatcaat atctacata tccaattgt tttagcgct caattgtta cattccttt acttaagac tgtaaataa ctggttaca tatccaaca aatatcatt taagcaagc ggcctgtgt atataataa caattgaaa agtacttaa tttcatggc aaattattt tcgaaaacg atcatgtga taattagtc ctttaatta tgtaaataa ttctattat ttgctcaat ttcgttccc ttttacgtc aattattct tcaaagggc cataaagca gcgttatgt aaataactc gcagtattt tatcctgat tttattatt acataatca taatgtact taatatagt gtactagac atgtaaatg taggccagt gtatgtatt tttataacg tgccttatt tgtgtatat aaaacgtta ataaaatta tgttgaaat c
- >TC005479 RB
tgcgttctt gtttcgcgt tgcttaaca tttatcgcg tcttgtgct agattactc catccatcc acttactca tcgccagtg tttcttcct ttttttgct cgcactcga tcgactttc gagaggaaa tcgatgggc gaacgtgac gcgaacaat taccgtaaa taatgcaat tattcgcga atttacgac caaatctaa cttttgcga tgatctctt caagacgtt tcaatcaag gacggatgt agcgaagcg gcaattaaa tttgcgttt tattaagaa aatgggaat ttcgccagt tatttatta atgtgtgag tttgaattc tttccacac tttctcaat tactttctt cgaaaaaat tactacatt atgctcatt cgagttgtg aaagatttt tacgcgaag ttcggaaac tttgtaagc attttcgta attaaatcg accgttttg tcatcacag tgtttattt tgtttccag attgtgtgg ctactattc ggacctcaa ctttaagag atgatggag tctctcaac ttcacacat ctcgccgtt tgcacgctc tcatccccc tttgacaaa cctcacctc ttgtgcacc tttctgtga taaaccgtt atcatagtg ttatcatgt caaaatgct aatccgaaa gtttagata agaagataa agacgtgga atagtgtgt gtgcgcgtg tgtaaatcc tcgaaatat tcgtgtaca agcctgacg atgggcgtg ttgtggagg aaacgttcg ttcttgttg aaactaatc gtgattgtg gggacggcc tggtttacg atagctttt ctcttgtac aatgacgac agaggctcg aatcgcatt ccggtgccg gtggacgat gaacaggac gcagtgccg cgagttttg cccttcagg agggaacct ccacaccgg gaggcgccc aagaaggag gaggagaag ccggtcttg ctaccgcca gcgagcaat gcaggggag atgggcaag ccggtggtg ctgccctcc aatctttca gccgacgta aagaagttg gtcgacgct ggctggcag aaaaacgcc ttcaaccaa tacgtcagc gatatgata agcgtccac cgaagttta ccggatccc agggacgaa tggtgtaag gctccgggt cgtttccag gaagccctc ccccaaacc tccgtcatt atttgtttc cacaatgag gcctggtct gtcctcctt cgaacagtt cattcagtc ctcgaccgc tccccttca catctcatc aaggaggtg atccttgtg gacgacttc tccgacatg gatcatctc aagcagcaa ctagtcgat tatttcgcc tccgaaccc aaagtcaag ataatccgt gcgaaaaaa cgcgaaggt ttgattaga gcccgtctt ctgggggcg gcacatgcc gaaggcgaa gttttgact tatctggac tcgcactgc gagtgcacg accggctgg ttggagccg cttttggat cggatagca agggatccg acgacggtg gtgtgtccg gttatagac gtaatcgat gatacaaca ttggagtat catttccat gatagtgga ggagtcaat gtgggaggg ttcgactgg aatttacag ttcaattgg cacgcggtg cccgaacac gagaagaag aggcacaag aatccggcc gaaccggtg tatagtccc accatggct gggggactt tttagcatt gataagaaa tttttcgaa cgtctggga acctacgac aacggcttc gacatttgg ggcggcgag aacttggag ctctccttc aagacgtgg atgtgcggt ggcactctc gaaatcgtt ccgtgctca cacgtcggt cacatcttc cgaaaacgt tcgccgtat aaatggcga agtggcgtg aacgtttta cgccgaaat tctgtccga ttggccgaa gtctggctg gacgaatac gccaagtat tactaccag cggataggc aacgaaaag ggcgatttc ggagacatc acctcgagg aaagcactc cgcgaaaag ctgggctgt aagagcttc aagtggtat ttggacaac atctatccg gagttgttt attccgggc gaagcggtc gccagtgga gagattcgc aacttgggc attggcgga aagacctgt ttggattct ccagctcgt cgcagcgac ttacacaaa cctgtgggc ttgtaccct tgccaccgc caaggcgga aatcagttc tggatgtac agtaaaagt ggtgagatc cgtcgagac gaggcctgc cttgactac agcggtcaa gaggtgatt ctgtacccg tgtcacggc agcaagggc aaccaattt tgggattac aacgccaac agcaaactt ttaaggcat ggaagtagc gataaatgt ttggccata aacgaagcg aaaaataaa ctactaatg gagccctgc gacgaagag gccacaagg caacactgg tcgttggag aactacgac gccagcaaa ttgtgattg ttttagttt atgtgtgtg tggcaaaaa gacgagtaa agtaagtgt acaaaagtt ttgtataaa tgatcggtt ttgcagttt tataacgaa ttgtagatt ctagcaacg actgtgtct atcaatatc tacatatcc aattgtttt agcgctcaa ttgttacat tcctttact taagactgt aaataactg gttacatat ccaacaaat atcatttaa gcaagcggc ctgtgtata taataacaa ttgaaaagt acttaattt catggcaaa ttattttcg aaaacgatc atgtgataa ttagtcctt taattatgt aaataattc tattatttg ctcaatttc gttcccttt tacgtcaat tattcttca aagggccat aaagcagcg ttatgtaaa taactcgca gtattttat cctgatttt attattaca taatcataa tgtacttaa tatagtgta ctagacatg taaatgtag gccagtgta tgtattttt ataacgtgc cttatttgt gtatataaa acgttaata aaattatgt tgaaatc
