Status
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Gene information
General information
TC005474 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12516594..12531879
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005474
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03960

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005474 RA
        MVDTQHFCL RWNNYQSSI TSAFENLRD DEDFVDVTL 
        ACDGKSLKA HRVVLSACS PYFRELLKS TPCKHPVIV 
        LQDVAWTDL HALVEFIYH GEVNVHQRS LSSFLKTAE 
        VLRVSGLTQ QHGDDREQL AQVQSLVRS QQSTPTSNH 
        HPSFTEKLV EDALFTSPS SPPHGATVN QLLRRAALQ 
        HRRERRISS DPEGDHKRP RPDHIIGNN NNNELNLSH 
        TQPPQTQPA DFSPSAMKN NALNLNMNS SSTTTTQNE 
        ANNGITSTE RENSPCSPS PTLNSRLNE ENVKSEPME 
        LLCSTNQEE NSNDSGDVA NDNGPNNLP QGPHSGSSA 
        GDHDEHDSP IGPYLTPSE SKLFATAAG SFNFSMAAL 
        AADPTGLGG LNQSLQANA DSLAGTSQA RRLSLPLPL 
        SATHRCDVC GKLLSTKLT LKRHKEQQH LQPLNNAVC 
        NLCHKVFRT LNSLNNHRS IYHRRQK
      • >TC005474 RB
        MVDTQHFCL RWNNYQSSI TSAFENLRD DEDFVDVTL 
        ACDGKSLKA HRVVLSACS PYFRELLKS TPCKHPVIV 
        LQDVAWTDL HALVEFIYH GEVNVHQRS LSSFLKTAE 
        VLRVSGLTQ QHGDDREQL AQVQSLVRS QQSTPTSNH 
        HPSFTEKLV EDALFTSPS SPPHGATVN QLLRRAALQ 
        HRRERRISS DPEGDHKRP RPDHIIGNN NNNELNLSH 
        TQPPQTQPA DFSPSAMKN NALNLNMNS SSTTTTQNE 
        ANNGITSTE RENSPCSPS PTLNSRLNE ENVKSEPME 
        LLCSTNQEE NSNDSGDVA NDNGPNNLP QGPHSGSSA 
        GDHDEHDSP IGPYLTPSE SKLFATAAG SFNFSMAAL 
        AADPTGLGG LNQSLQANA DSLAGTSQE SSGGGRPGR 
        TAFCDICNK TFSRFWSLQ RHLSDTHFY TPQNLQCNV 
        CGRSYRSRN SLVSHRSQY HREGNEIKF ENDCFI
      CDS sequence
      • >TC005474 RA
        atggtagat acacaacac ttctgtctg cggtggaac 
        aattaccaa agcagcatc acatcggct ttcgagaat 
        ctccgggat gatgaagat tttgtcgat gttacgttg 
        gcctgcgat ggaaaaagt ctaaaagct catcgtgtt 
        gttttatcg gcttgcagt ccctatttt cgggaatta 
        cttaagtct actccttgc aaacaccct gtgatagtc 
        ctgcaagat gttgcgtgg acggacttg cacgccctc 
        gtcgaattt atctaccat ggcgaagtc aacgtgcac 
        cagcggtct ctttcgtcg tttctcaag acggctgaa 
        gtgctccgg gtcagcggg ctcacccaa cagcacggc 
        gacgaccgc gaacagttg gcgcaagta caaagcctg 
        gtgcgatct cagcagtcg acaccgacg tcgaaccac 
        caccccagc ttcaccgag aagttagtg gaggacgcc 
        ctcttcacc tccccctca tctccccct cacggcgcc 
        accgtcaac cagctcctc cgtcgagcc gctttacag 
        caccgccgc gagcgtcgg atatcctcc gatccggag 
        ggcgaccac aagcggccc cgtcccgac cacatcatc 
        ggcaacaac aacaataac gaactgaat ctatcgcac 
        acccaaccg ccccaaacc caaccggcg gacttctct 
        ccaagcgcg atgaaaaac aacgctctt aatctcaat 
        atgaactcc tcgtcgacg acgacgacg caaaacgag 
        gccaacaac ggcatcacg agcacagaa cgggagaat 
        tcgccgtgt tcgccgtcg ccgacgctc aattcgcgc 
        ctgaacgag gagaacgtg aagagcgaa ccgatggag 
        ctgctgtgt agtacaaat caggaagag aatagtaac 
        gactcgggc gatgtggcc aacgacaac gggcctaat 
        aatctacct caagggccg cattctggg agttcggct 
        ggggaccat gatgaacac gacagtccg attgggccc 
        tatcttacg ccgtcggag agcaagctg tttgcgacg 
        gcagcggga agcttcaac tttagtatg gcggcgctg 
        gcggcggat ccgacggga cttggaggt ttaaatcaa 
        tctctgcag gcgaacgct gacagtcta gcaggcact 
        tcacaagct agaaggttg agtcttcca cttccactg 
        agcgccacc catcgctgt gacgtctgc gggaagctc 
        ctcagcacc aaactcact ctgaaaagg cacaaggaa 
        caacaacat cttcagccg ctcaacaac gccgtgtgt 
        aatctctgt cacaaagtg tttcggaca ctgaattcg 
        cttaacaat catcgcagt atttaccat cgcagacaa 
        aaataa
      • >TC005474 RB
        atggtagat acacaacac ttctgtctg cggtggaac 
        aattaccaa agcagcatc acatcggct ttcgagaat 
        ctccgggat gatgaagat tttgtcgat gttacgttg 
        gcctgcgat ggaaaaagt ctaaaagct catcgtgtt 
        gttttatcg gcttgcagt ccctatttt cgggaatta 
        cttaagtct actccttgc aaacaccct gtgatagtc 
        ctgcaagat gttgcgtgg acggacttg cacgccctc 
        gtcgaattt atctaccat ggcgaagtc aacgtgcac 
        cagcggtct ctttcgtcg tttctcaag acggctgaa 
        gtgctccgg gtcagcggg ctcacccaa cagcacggc 
        gacgaccgc gaacagttg gcgcaagta caaagcctg 
        gtgcgatct cagcagtcg acaccgacg tcgaaccac 
        caccccagc ttcaccgag aagttagtg gaggacgcc 
        ctcttcacc tccccctca tctccccct cacggcgcc 
        accgtcaac cagctcctc cgtcgagcc gctttacag 
        caccgccgc gagcgtcgg atatcctcc gatccggag 
        ggcgaccac aagcggccc cgtcccgac cacatcatc 
        ggcaacaac aacaataac gaactgaat ctatcgcac 
        acccaaccg ccccaaacc caaccggcg gacttctct 
        ccaagcgcg atgaaaaac aacgctctt aatctcaat 
        atgaactcc tcgtcgacg acgacgacg caaaacgag 
        gccaacaac ggcatcacg agcacagaa cgggagaat 
        tcgccgtgt tcgccgtcg ccgacgctc aattcgcgc 
        ctgaacgag gagaacgtg aagagcgaa ccgatggag 
        ctgctgtgt agtacaaat caggaagag aatagtaac 
        gactcgggc gatgtggcc aacgacaac gggcctaat 
        aatctacct caagggccg cattctggg agttcggct 
        ggggaccat gatgaacac gacagtccg attgggccc 
        tatcttacg ccgtcggag agcaagctg tttgcgacg 
        gcagcggga agcttcaac tttagtatg gcggcgctg 
        gcggcggat ccgacggga cttggaggt ttaaatcaa 
        tctctgcag gcgaacgct gacagtcta gcaggcact 
        tcacaagag tcgtcgggg ggcggaaga ccgggcagg 
        acggcgttt tgcgacatt tgcaataaa accttctca 
        cggttttgg tccctccaa cgtcatcta tctgacacg 
        cacttttac acgccacaa aatctacaa tgcaacgtt 
        tgcggaagg tcgtacaga tcacggaac tcgcttgtc 
        agccatcgg agtcagtat cacagggaa ggaaacgag 
        atcaaattt gagaatgac tgcttcatc taa
      mRNA sequence
      • >TC005474 RA
        tcagcgaat tcgggcgtt aaaatatct ggggttgta 
        cgagccgcc cctgtcgcg gcgaatatg cagttttga 
        tcttttctt tatcgtcaa gtcgcgagc tggtgcaaa 
        aaatcgaaa tttggtgca aataagtcg aagatgtgg 
        tgtggcgac tgtatgtgg gagtgttgg gtggaaagc 
        gtcgggaaa tggtcgtgt cggaggtga tgcagggcg 
        cagatttgg tacacttcg attttttta ttttgacat 
        ttttaattg cgggcctgt actcgttat tctgcgcat 
        tttcattcg tcgcgggag gggtaggcg ggcacgcac 
        tatttttta gtgccggct tattacagc agatgtctc 
        atagccatg tcccaataa agaataagc ggcccgtca 
        ttttttgcg cgcttgaat ttttaatat tcgcccgaa 
        tgtcgtgac gtactgacg tcggcccgt gcacagtgt 
        gataacaaa acagttcgc ttttcgtat tttggcggt 
        ctagtgtgc cttggaaga cgcttctga catctgtgc 
        atcgcgacg cacagtcgc gaaaatgtc catgtgagg 
        aggaaaaaa tggacgcgc cgaaaaaaa acacactca 
        gagacataa atgaccccg aattttaac aggtgaata 
        gtgattacg atggtagat acacaacac ttctgtctg 
        cggtggaac aattaccaa agcagcatc acatcggct 
        ttcgagaat ctccgggat gatgaagat tttgtcgat 
        gttacgttg gcctgcgat ggaaaaagt ctaaaagct 
        catcgtgtt gttttatcg gcttgcagt ccctatttt 
        cgggaatta cttaagtct actccttgc aaacaccct 
        gtgatagtc ctgcaagat gttgcgtgg acggacttg 
        cacgccctc gtcgaattt atctaccat ggcgaagtc 
        aacgtgcac cagcggtct ctttcgtcg tttctcaag 
        acggctgaa gtgctccgg gtcagcggg ctcacccaa 
        cagcacggc gacgaccgc gaacagttg gcgcaagta 
        caaagcctg gtgcgatct cagcagtcg acaccgacg 
        tcgaaccac caccccagc ttcaccgag aagttagtg 
        gaggacgcc ctcttcacc tccccctca tctccccct 
        cacggcgcc accgtcaac cagctcctc cgtcgagcc 
        gctttacag caccgccgc gagcgtcgg atatcctcc 
        gatccggag ggcgaccac aagcggccc cgtcccgac 
        cacatcatc ggcaacaac aacaataac gaactgaat 
        ctatcgcac acccaaccg ccccaaacc caaccggcg 
        gacttctct ccaagcgcg atgaaaaac aacgctctt 
        aatctcaat atgaactcc tcgtcgacg acgacgacg 
        caaaacgag gccaacaac ggcatcacg agcacagaa 
        cgggagaat tcgccgtgt tcgccgtcg ccgacgctc 
        aattcgcgc ctgaacgag gagaacgtg aagagcgaa 
        ccgatggag ctgctgtgt agtacaaat caggaagag 
        aatagtaac gactcgggc gatgtggcc aacgacaac 
        gggcctaat aatctacct caagggccg cattctggg 
        agttcggct ggggaccat gatgaacac gacagtccg 
        attgggccc tatcttacg ccgtcggag agcaagctg 
        tttgcgacg gcagcggga agcttcaac tttagtatg 
        gcggcgctg gcggcggat ccgacggga cttggaggt 
        ttaaatcaa tctctgcag gcgaacgct gacagtcta 
        gcaggcact tcacaagct agaaggttg agtcttcca 
        cttccactg agcgccacc catcgctgt gacgtctgc 
        gggaagctc ctcagcacc aaactcact ctgaaaagg 
        cacaaggaa caacaacat cttcagccg ctcaacaac 
        gccgtgtgt aatctctgt cacaaagtg tttcggaca 
        ctgaattcg cttaacaat catcgcagt atttaccat 
        cgcagacaa aaataagaa cgaggttaa agacagtca 
        cctatgtta agcccccgc tgcattaca agtctagcg 
        gcaagcaaa cacacttgt aattttact cataatgga 
        ggtccggtc tgtgcaaat atagggggc ggctttagt 
        ttgttaagt ctccattat tctgttgag tccacgtta 
        cattcggtt catttggct ctgtgatat tttttgttg 
        gaaatcggc caggagctg gcgattttt accacccca 
        gccaccaaa attgcgacc agttttatt ttacttggt 
        ggttgggat attggtgca aaattggcc gtccgtgga 
        cgagttatt taaaattgt aacgcggat cgaaattga 
        caaaatcaa agccttttt ataattata atcggtgaa 
        agggaattc ttccattta tacatataa tatacaata 
        tttaaagaa gaatatatt agaataata tatgtttat 
        ttatctctt ttctttatt aataatgat gagttatgc 
        tctggctcg gagcgtagt ttgtatttc tatgagacg 
        aaataaatt gttgtacga gagacagga cttgagttt 
        cttaaaaga ctgaaatta gtttaaatg ataatttgc 
        ggtattctt taagactga gtatatttt gactgattt 
        ttttggtaa gaaagattg gcaaccccg tgaattgta 
        atttggtcc tgtgtagta agacataga atttttttc 
        atttttttg catttacgc atgttcatg ttgtttgat 
        gcaatattt atcatattt tatgtactg ttgtataac 
        ctttattta tgtttaaat atattttta caatttgca 
        aaagcagct tgggtttta ttttgcttg tttcgtgag 
        cctttccct cattgttta tgtgttcga aacccaccc 
        ccaacacga gagtgttgc caccaggag actgttgtt 
        tctgcgtga ccccgccgc gccatcatt cgtccgccc 
        gatgacacg acgtaacgc atggacggg attttcgag 
        tttttagtt cggttgttg acggtgcgt tttgtcggg 
        cagcggggt cgtggatag attaggttt ggggcgcgg 
        cggagcagc tggcagact ctttcgtcg agatatgat 
        aatgtgtgt ggagacgat catttaact aaatgtgtc 
        tgcgaaca
      • >TC005474 RB
        tcagcgaat tcgggcgtt aaaatatct ggggttgta 
        cgagccgcc cctgtcgcg gcgaatatg cagttttga 
        tcttttctt tatcgtcaa gtcgcgagc tggtgcaaa 
        aaatcgaaa tttggtgca aataagtcg aagatgtgg 
        tgtggcgac tgtatgtgg gagtgttgg gtggaaagc 
        gtcgggaaa tggtcgtgt cggaggtga tgcagggcg 
        cagatttgg tacacttcg attttttta ttttgacat 
        ttttaattg cgggcctgt actcgttat tctgcgcat 
        tttcattcg tcgcgggag gggtaggcg ggcacgcac 
        tatttttta gtgccggct tattacagc agatgtctc 
        atagccatg tcccaataa agaataagc ggcccgtca 
        ttttttgcg cgcttgaat ttttaatat tcgcccgaa 
        tgtcgtgac gtactgacg tcggcccgt gcacagtgt 
        gataacaaa acagttcgc ttttcgtat tttggcggt 
        ctagtgtgc cttggaaga cgcttctga catctgtgc 
        atcgcgacg cacagtcgc gaaaatgtc catgtgagg 
        aggaaaaaa tggacgcgc cgaaaaaaa acacactca 
        gagacataa atgaccccg aattttaac aggtgaata 
        gtgattacg atggtagat acacaacac ttctgtctg 
        cggtggaac aattaccaa agcagcatc acatcggct 
        ttcgagaat ctccgggat gatgaagat tttgtcgat 
        gttacgttg gcctgcgat ggaaaaagt ctaaaagct 
        catcgtgtt gttttatcg gcttgcagt ccctatttt 
        cgggaatta cttaagtct actccttgc aaacaccct 
        gtgatagtc ctgcaagat gttgcgtgg acggacttg 
        cacgccctc gtcgaattt atctaccat ggcgaagtc 
        aacgtgcac cagcggtct ctttcgtcg tttctcaag 
        acggctgaa gtgctccgg gtcagcggg ctcacccaa 
        cagcacggc gacgaccgc gaacagttg gcgcaagta 
        caaagcctg gtgcgatct cagcagtcg acaccgacg 
        tcgaaccac caccccagc ttcaccgag aagttagtg 
        gaggacgcc ctcttcacc tccccctca tctccccct 
        cacggcgcc accgtcaac cagctcctc cgtcgagcc 
        gctttacag caccgccgc gagcgtcgg atatcctcc 
        gatccggag ggcgaccac aagcggccc cgtcccgac 
        cacatcatc ggcaacaac aacaataac gaactgaat 
        ctatcgcac acccaaccg ccccaaacc caaccggcg 
        gacttctct ccaagcgcg atgaaaaac aacgctctt 
        aatctcaat atgaactcc tcgtcgacg acgacgacg 
        caaaacgag gccaacaac ggcatcacg agcacagaa 
        cgggagaat tcgccgtgt tcgccgtcg ccgacgctc 
        aattcgcgc ctgaacgag gagaacgtg aagagcgaa 
        ccgatggag ctgctgtgt agtacaaat caggaagag 
        aatagtaac gactcgggc gatgtggcc aacgacaac 
        gggcctaat aatctacct caagggccg cattctggg 
        agttcggct ggggaccat gatgaacac gacagtccg 
        attgggccc tatcttacg ccgtcggag agcaagctg 
        tttgcgacg gcagcggga agcttcaac tttagtatg 
        gcggcgctg gcggcggat ccgacggga cttggaggt 
        ttaaatcaa tctctgcag gcgaacgct gacagtcta 
        gcaggcact tcacaagag tcgtcgggg ggcggaaga 
        ccgggcagg acggcgttt tgcgacatt tgcaataaa 
        accttctca cggttttgg tccctccaa cgtcatcta 
        tctgacacg cacttttac acgccacaa aatctacaa 
        tgcaacgtt tgcggaagg tcgtacaga tcacggaac 
        tcgcttgtc agccatcgg agtcagtat cacagggaa 
        ggaaacgag atcaaattt gagaatgac tgcttcatc 
        taaaactag cgcccggaa atataaatt attactcta 
        ggattagag aaaatgact catttatta ttttatcga 
        taattgttt gctggttgg tttcattac gaattggga 
        ttgttttct ctaattcat cgtcgcagc aattgaaga 
        atgttacga aacgaagat gatcagcaa aaattcggc 
        ggattttga gtgaacggg ctaacagag agtgtgata 
        cagtgtgag atattaaat aatagtcgg acgcaagag 
        aattttctg gttaaataa attatcgtt ttattatga 
        acattcggg actgaactt tttttgtgt atgtcagat 
        ttcgatgac caacctgtt aatttgtca cacgcttat 
        agaatttat taaacggtc atctttaga ggaaatttg 
        gtgttttct tttaaattt gtgataaaa tggaggcaa 
        atttttggt ttggtttgt ttggtacag gatgttcca 
        gaaataatt gaatcaaaa acaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY