Status
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Gene information
General information
TC031289
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005465,TC005466
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12682652..12690504
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031289
    iBeetle screen iB_03958

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    elytra orientation irregular - Number of animals showing the phenotype: 2


    elytra pigmentation/tanning decreased - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031289 RA
      MGNTSPRPP ALNQDSVDG VYTQRREDS CQNLSTSSS 
      GRSFASVNS HQSTFSVSR PWSRVSRRR WKESTLTLP 
      YESSKTAWP VSQIESFFL PEFPFRSEG KHFTNLGEI 
      NKGAFGKVY KVSDEKGTL YALKVLSKS KIIRENAVQ 
      QVKDEVRIQ RVCGHHPFI VDCPFHWQS KKRLFIVSN 
      YVAGGELYD LLKTYTVLP VALVKLYVA QLALALDFL 
      HNAGVIYRD LKPENILLD ASLNVQLID FGLSKWLPY 
      GKTTRTICG TFLYMGMKS EKMNARSQM FDLAVEGHQ 
      ADEAVASIF HTVLFHRTH GKFLYSTEG SYSIGTIGY 
      TDVDCDFID LTYVCCSSK RLDETLKRE ISLFSEQLR 
      GNEGTGMGQ ISLEFFQKK PRSRWLFQP ESIPWEVWT 
      VRLELITLA SEGERQVYR EQMGELLAD KIFSIAEIM 
      NRHDYLPKM PSQSELDLI FDTSYPDCQ PYLFKVNYS 
      ISGPSSGSS VGSTVKKLI KETLAL
    • >TC031289 RB
      MGNTSPRPP ALNQDSVDG VYTQRREDS CQNLSTSSS 
      GRSFASVNS HQSTFSVSR PWSRVSRRR WKESTLTLP 
      YESSKTAWP VSQIESFFL PEFPFRSEG KHFTNLGEI 
      NKGAFGKVY KVSDEKGTL YALKVLSKS KIIRENAVQ 
      QVKDEVRIQ RVCGHHPFI VDCPFHWQS KKRLFIVSN 
      YVAGGELYD LLKTYTVLP VALVKLYVA QLALALDFL 
      HNAGVIYRD LKPENILLD ASLNVQLID FGLSKWLPY 
      GKTTRTICG TFLYMAPEV WTGEEYGHG VDWWSLGVI 
      ACVMLTGKH SIDGQRLAA AASEHDQIR SSY
    • >TC031289 RC
      MNARSQMFD LAVEGHQAD EAVASIFHT VLFHRTHGK 
      FLYSTEGSY SIGTIGYTD VDCDFIDLT YVCCSSKRL 
      DETLKREIS LFSEQLRGN EGTGMGQIS LEFFQKKPR 
      SRWLFQPES IPWEVWTVR LELITLASE GERQVYREQ 
      MGELLADKI FSIAEIMNR HDYLPKMPS QSELDLIFD 
      TSYPDCQPY LFKVNYSIS GPSSGSSVG STVKKLIKE 
      TLAL
    CDS sequence
    • >TC031289 RA
      atgggaaat acaagtccg aggccccca gctttgaat 
      caagacagt gttgacggt gtttacaca caaagacgt 
      gaggattca tgtcagaat ttgagtacg agttcgagt 
      ggacggtcg tttgcgagt gttaacagc caccaatca 
      accttcagt gtctcgagg ccttggtcg cgggtctcc 
      agacgcagg tggaaggaa tcgactcta actttaccg 
      tacgagtcg agcaaaacc gcttggcct gtttcgcaa 
      atcgagtcg tttttcttg cccgagttt ccgtttcgt 
      agcgaaggc aagcatttt acgaatttg ggcgaaata 
      aacaaaggg gcgtttggc aaagtgtac aaagtgagc 
      gacgagaaa gggacgctt tacgcgctc aaagtctta 
      tctaaatct aagattatt agagagaac gctgtacag 
      caagttaaa gacgaagtg aggattcag agggtgtgt 
      gggcatcat ccgtttatc gttgattgt ccgtttcat 
      tggcagagt aagaagagg ttgtttatc gtaagtaat 
      tacgttgcc ggtggggaa ctttatgat ttattgaag 
      acttacacc gtccttcct gtggcttta gtcaagctt 
      tacgttgca caattggcc cttgctctt gattttttg 
      cacaacgcc ggcgtgatt taccgcgac ctgaaaccg 
      gaaaacatc ctcttagat gcctctctc aacgtccaa 
      ttgattgat ttcggcctt tcgaagtgg ttgccatac 
      ggcaagaca acgcgtacg atttgcggc accttttta 
      tacatgggt atgaaatcc gaaaaaatg aacgccaga 
      tctcagatg ttcgatttg gcggtggag ggccaccaa 
      gccgacgaa gccgttgct agtattttc cacacagtg 
      ttgtttcat cgaacgcat ggaaagttc ctctacagc 
      acggaaggg agctattcc atcggaacg atcggctac 
      accgatgta gattgcgac tttatcgac ctgacttac 
      gtgtgttgt tcgtcgaaa cggctcgat gagacgctc 
      aaacgtgag ataagttta tttagcgaa caattgcgg 
      ggcaacgaa ggcactggc atgggtcag attagtttg 
      gagttcttc caaaagaag ccacgaagc cggtggttg 
      ttccaaccg gagagcatc ccctgggag gtgtggacg 
      gtgcgtctg gagctcatc acgttggcc agtgagggc 
      gagagacaa gtttacagg gagcaaatg ggggaatta 
      ctcgccgac aaaattttc tcgatagct gaaattatg 
      aatcggcac gattatttg ccgaaaatg ccgagtcag 
      tccgagttg gatttgatc ttcgatacg tcgtatccc 
      gattgtcag ccctacttg tttaaggtg aattattca 
      atttcgggt ccttcgtca ggctcttcg gtcggctcg 
      accgttaag aagttaatc aaagagact ctagcactt 
      taa
    • >TC031289 RB
      atgggaaat acaagtccg aggccccca gctttgaat 
      caagacagt gttgacggt gtttacaca caaagacgt 
      gaggattca tgtcagaat ttgagtacg agttcgagt 
      ggacggtcg tttgcgagt gttaacagc caccaatca 
      accttcagt gtctcgagg ccttggtcg cgggtctcc 
      agacgcagg tggaaggaa tcgactcta actttaccg 
      tacgagtcg agcaaaacc gcttggcct gtttcgcaa 
      atcgagtcg tttttcttg cccgagttt ccgtttcgt 
      agcgaaggc aagcatttt acgaatttg ggcgaaata 
      aacaaaggg gcgtttggc aaagtgtac aaagtgagc 
      gacgagaaa gggacgctt tacgcgctc aaagtctta 
      tctaaatct aagattatt agagagaac gctgtacag 
      caagttaaa gacgaagtg aggattcag agggtgtgt 
      gggcatcat ccgtttatc gttgattgt ccgtttcat 
      tggcagagt aagaagagg ttgtttatc gtaagtaat 
      tacgttgcc ggtggggaa ctttatgat ttattgaag 
      acttacacc gtccttcct gtggcttta gtcaagctt 
      tacgttgca caattggcc cttgctctt gattttttg 
      cacaacgcc ggcgtgatt taccgcgac ctgaaaccg 
      gaaaacatc ctcttagat gcctctctc aacgtccaa 
      ttgattgat ttcggcctt tcgaagtgg ttgccatac 
      ggcaagaca acgcgtacg atttgcggc accttttta 
      tacatggct ccggaggta tggacgggg gaggagtat 
      gggcatgga gttgattgg tggagtctg ggagtgatt 
      gcctgtgtg atgctgacc gggaagcat tccatcgac 
      ggtcaaaga ctcgctgct gctgcatcc gaacatgac 
      caaataagg tctagctac tag
    • >TC031289 RC
      atgaacgcc agatctcag atgttcgat ttggcggtg 
      gagggccac caagccgac gaagccgtt gctagtatt 
      ttccacaca gtgttgttt catcgaacg catggaaag 
      ttcctctac agcacggaa gggagctat tccatcgga 
      acgatcggc tacaccgat gtagattgc gactttatc 
      gacctgact tacgtgtgt tgttcgtcg aaacggctc 
      gatgagacg ctcaaacgt gagataagt ttatttagc 
      gaacaattg cggggcaac gaaggcact ggcatgggt 
      cagattagt ttggagttc ttccaaaag aagccacga 
      agccggtgg ttgttccaa ccggagagc atcccctgg 
      gaggtgtgg acggtgcgt ctggagctc atcacgttg 
      gccagtgag ggcgagaga caagtttac agggagcaa 
      atgggggaa ttactcgcc gacaaaatt ttctcgata 
      gctgaaatt atgaatcgg cacgattat ttgccgaaa 
      atgccgagt cagtccgag ttggatttg atcttcgat 
      acgtcgtat cccgattgt cagccctac ttgtttaag 
      gtgaattat tcaatttcg ggtccttcg tcaggctct 
      tcggtcggc tcgaccgtt aagaagtta atcaaagag 
      actctagca ctttaa
    mRNA sequence
    • >TC031289 RA
      ttaccttaa ttttgaggt cagagtaga gtggaaatg 
      tttgttgtt ttcggtggg aaaaagggc cccagtcgt 
      cacctgcca ctggtcttt acatgggaa atacaagtc 
      cgaggcccc cagctttga atcaagaca gtgttgacg 
      gtgtttaca cacaaagac gtgaggatt catgtcaga 
      atttgagta cgagttcga gtggacggt cgtttgcga 
      gtgttaaca gccaccaat caaccttca gtgtctcga 
      ggccttggt cgcgggtct ccagacgca ggtggaagg 
      aatcgactc taactttac cgtacgagt cgagcaaaa 
      ccgcttggc ctgtttcgc aaatcgagt cgtttttct 
      tgcccgagt ttccgtttc gtagcgaag gcaagcatt 
      ttacgaatt tgggcgaaa taaacaaag gggcgtttg 
      gcaaagtgt acaaagtga gcgacgaga aagggacgc 
      tttacgcgc tcaaagtct tatctaaat ctaagatta 
      ttagagaga acgctgtac agcaagtta aagacgaag 
      tgaggattc agagggtgt gtgggcatc atccgttta 
      tcgttgatt gtccgtttc attggcaga gtaagaaga 
      ggttgttta tcgtaagta attacgttg ccggtgggg 
      aactttatg atttattga agacttaca ccgtccttc 
      ctgtggctt tagtcaagc tttacgttg cacaattgg 
      cccttgctc ttgattttt tgcacaacg ccggcgtga 
      tttaccgcg acctgaaac cggaaaaca tcctcttag 
      atgcctctc tcaacgtcc aattgattg atttcggcc 
      tttcgaagt ggttgccat acggcaaga caacgcgta 
      cgatttgcg gcacctttt tatacatgg gtatgaaat 
      ccgaaaaaa tgaacgcca gatctcaga tgttcgatt 
      tggcggtgg agggccacc aagccgacg aagccgttg 
      ctagtattt tccacacag tgttgtttc atcgaacgc 
      atggaaagt tcctctaca gcacggaag ggagctatt 
      ccatcggaa cgatcggct acaccgatg tagattgcg 
      actttatcg acctgactt acgtgtgtt gttcgtcga 
      aacggctcg atgagacgc tcaaacgtg agataagtt 
      tatttagcg aacaattgc ggggcaacg aaggcactg 
      gcatgggtc agattagtt tggagttct tccaaaaga 
      agccacgaa gccggtggt tgttccaac cggagagca 
      tcccctggg aggtgtgga cggtgcgtc tggagctca 
      tcacgttgg ccagtgagg gcgagagac aagtttaca 
      gggagcaaa tgggggaat tactcgccg acaaaattt 
      tctcgatag ctgaaatta tgaatcggc acgattatt 
      tgccgaaaa tgccgagtc agtccgagt tggatttga 
      tcttcgata cgtcgtatc ccgattgtc agccctact 
      tgtttaagg tgaattatt caatttcgg gtccttcgt 
      caggctctt cggtcggct cgaccgtta agaagttaa 
      tcaaagaga ctctagcac tttaaaaca attttttct 
      agtagaatt tgtcaatta ggaccgctt cagcgattt 
      ttctttttt catgacgtt gtttgagat tttttcgct 
      agggtagta gtttttagg atta
    • >TC031289 RB
      ttaccttaa ttttgaggt cagagtaga gtggaaatg 
      tttgttgtt ttcggtggg aaaaagggc cccagtcgt 
      cacctgcca ctggtcttt acatgggaa atacaagtc 
      cgaggcccc cagctttga atcaagaca gtgttgacg 
      gtgtttaca cacaaagac gtgaggatt catgtcaga 
      atttgagta cgagttcga gtggacggt cgtttgcga 
      gtgttaaca gccaccaat caaccttca gtgtctcga 
      ggccttggt cgcgggtct ccagacgca ggtggaagg 
      aatcgactc taactttac cgtacgagt cgagcaaaa 
      ccgcttggc ctgtttcgc aaatcgagt cgtttttct 
      tgcccgagt ttccgtttc gtagcgaag gcaagcatt 
      ttacgaatt tgggcgaaa taaacaaag gggcgtttg 
      gcaaagtgt acaaagtga gcgacgaga aagggacgc 
      tttacgcgc tcaaagtct tatctaaat ctaagatta 
      ttagagaga acgctgtac agcaagtta aagacgaag 
      tgaggattc agagggtgt gtgggcatc atccgttta 
      tcgttgatt gtccgtttc attggcaga gtaagaaga 
      ggttgttta tcgtaagta attacgttg ccggtgggg 
      aactttatg atttattga agacttaca ccgtccttc 
      ctgtggctt tagtcaagc tttacgttg cacaattgg 
      cccttgctc ttgattttt tgcacaacg ccggcgtga 
      tttaccgcg acctgaaac cggaaaaca tcctcttag 
      atgcctctc tcaacgtcc aattgattg atttcggcc 
      tttcgaagt ggttgccat acggcaaga caacgcgta 
      cgatttgcg gcacctttt tatacatgg ctccggagg 
      tatggacgg gggaggagt atgggcatg gagttgatt 
      ggtggagtc tgggagtga ttgcctgtg tgatgctga 
      ccgggaagc attccatcg acggtcaaa gactcgctg 
      ctgctgcat ccgaacatg accaaataa ggtctagct 
      actagggca agaagaaag aaataaaac aacg
    • >TC031289 RC
      cgtcaacaa actcttaat taatttaag tgatcggtt 
      tgttatgtc gaattaagc gaaaaaatg aacgccaga 
      tctcagatg ttcgatttg gcggtggag ggccaccaa 
      gccgacgaa gccgttgct agtattttc cacacagtg 
      ttgtttcat cgaacgcat ggaaagttc ctctacagc 
      acggaaggg agctattcc atcggaacg atcggctac 
      accgatgta gattgcgac tttatcgac ctgacttac 
      gtgtgttgt tcgtcgaaa cggctcgat gagacgctc 
      aaacgtgag ataagttta tttagcgaa caattgcgg 
      ggcaacgaa ggcactggc atgggtcag attagtttg 
      gagttcttc caaaagaag ccacgaagc cggtggttg 
      ttccaaccg gagagcatc ccctgggag gtgtggacg 
      gtgcgtctg gagctcatc acgttggcc agtgagggc 
      gagagacaa gtttacagg gagcaaatg ggggaatta 
      ctcgccgac aaaattttc tcgatagct gaaattatg 
      aatcggcac gattatttg ccgaaaatg ccgagtcag 
      tccgagttg gatttgatc ttcgatacg tcgtatccc 
      gattgtcag ccctacttg tttaaggtg aattattca 
      atttcgggt ccttcgtca ggctcttcg gtcggctcg 
      accgttaag aagttaatc aaagagact ctagcactt 
      taaaacaat tttttctag tagaatttg tcaattagg 
      accgcttca gcgattttt cttttttca tgacgttgt 
      ttgagattt tttcgctag ggtagtagt ttttaggat 
      tatttattt ttagtttga gggtgcagc tgttttcga 
      cgtgatttt tctagttta gtttgtcta cggaggact 
      atgggttag gagacggaa cgggattca aaatctgga 
      cc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY