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Gene information
General information
TC005461 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12758375..12759676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005461
    iBeetle screen iB_03957

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005461 RA
      MGILEKISE IEKEISRTQ KNKATEYHL GLLKAKLAK 
      YRSQLLEPS KKSEKGEGF DVLKSGDAR VALIGFPSV 
      GKSTLLSTL TKTESEAAS YEFTTLTCI PGVIDYNGA 
      NIQLLDLPG IIEGAAQGK GRGRQVIAV ARTADLVLM 
      MLDATKKDV HRHLLEKEL ESVGIRLNK MKPNIYFKV 
      KKGGGISFN STCPLTKID EKLVQMILH EYKIFNAEV 
      LFREDCTAD ELIDVICAN RVYLPCLYV YNKIDQISI 
      EEVDRIARQ PNSVVVSCN MKLNLDYLL QVLWEYLNL 
      IRVYTKKPG QPPDFSDGL ILRKGVTVE HVCHAIHRT 
      LAQQFKYAL VWGTSTKYS PQRVGTSHV MHDEDVIQI 
      VKK
    CDS sequence
    • >TC005461 RA
      atgggaatt ttagaaaaa atctcggaa atcgagaag 
      gaaatctct cgcacgcaa aaaaataaa gccaccgag 
      taccactta ggtctcctc aaagccaaa cttgcaaaa 
      taccgttcc cagctcttg gagccttcg aaaaaatcg 
      gaaaagggt gaaggtttc gacgtttta aaaagtggg 
      gatgcgcgt gtggccttg atcggcttc ccgtcagtc 
      ggaaaatcc actttgttg tcaacttta acgaagacg 
      gaaagcgag gccgcgtcg tacgagttc acgaccttg 
      acgtgcatc ccgggcgtg atcgactac aatggggcc 
      aatatccag ctgctcgat ttgcccggg attatcgag 
      ggggctgcc cagggcaag ggcaggggg cggcaggtg 
      atagcggtg gcgcgcacg gccgacttg gtcctcatg 
      atgctggac gccacgaaa aaggacgtc caccgacac 
      ttattagaa aaggaattg gaaagtgtg gggattagg 
      ctgaacaaa atgaagccc aacatttat ttcaaggtg 
      aaaaaaggg ggcggtatt tcgttcaat tccacttgt 
      cccttgacg aaaatcgac gaaaagctc gtgcaaatg 
      attctgcac gagtacaaa atcttcaac gctgaggtt 
      ctcttcagg gaagactgt actgccgat gagttaatc 
      gacgtgatt tgtgcgaac cgcgtctat cttccatgc 
      ctttacgtt tataataaa atcgatcag atttcgatc 
      gaggaagtc gatagaata gccagacag ccgaacagt 
      gttgtcgtc agttgcaac atgaagctc aacctagat 
      tacctcctt caagtcctc tgggagtat ttaaattta 
      attcgcgtt tatacgaaa aaaccggga cagccgccg 
      gatttcagc gacggcttg attctgaga aagggggtt 
      acagtggag catgtgtgt cacgcaatc cacagaacg 
      ctcgcccag caattcaag tatgcctta gtctggggc 
      acgagcacc aaatactcg ccccaaagg gtcggcact 
      tcccacgtc atgcacgac gaggacgtc atccaaata 
      gttaagaag taa
    mRNA sequence
    • >TC005461 RA
      gaataaaac gtgccagaa aatgggaat tttagaaaa 
      aatctcgga aatcgagaa ggaaatctc tcgcacgca 
      aaaaaataa agccaccga gtaccactt aggtctcct 
      caaagccaa acttgcaaa ataccgttc ccagctctt 
      ggagccttc gaaaaaatc ggaaaaggg tgaaggttt 
      cgacgtttt aaaaagtgg ggatgcgcg tgtggcctt 
      gatcggctt cccgtcagt cggaaaatc cactttgtt 
      gtcaacttt aacgaagac ggaaagcga ggccgcgtc 
      gtacgagtt cacgacctt gacgtgcat cccgggcgt 
      gatcgacta caatggggc caatatcca gctgctcga 
      tttgcccgg gattatcga gggggctgc ccagggcaa 
      gggcagggg gcggcaggt gatagcggt ggcgcgcac 
      ggccgactt ggtcctcat gatgctgga cgccacgaa 
      aaaggacgt ccaccgaca cttattaga aaaggaatt 
      ggaaagtgt ggggattag gctgaacaa aatgaagcc 
      caacattta tttcaaggt gaaaaaagg gggcggtat 
      ttcgttcaa ttccacttg tcccttgac gaaaatcga 
      cgaaaagct cgtgcaaat gattctgca cgagtacaa 
      aatcttcaa cgctgaggt tctcttcag ggaagactg 
      tactgccga tgagttaat cgacgtgat ttgtgcgaa 
      ccgcgtcta tcttccatg cctttacgt ttataataa 
      aatcgatca gatttcgat cgaggaagt cgatagaat 
      agccagaca gccgaacag tgttgtcgt cagttgcaa 
      catgaagct caacctaga ttacctcct tcaagtcct 
      ctgggagta tttaaattt aattcgcgt ttatacgaa 
      aaaaccggg acagccgcc ggatttcag cgacggctt 
      gattctgag aaagggggt tacagtgga gcatgtgtg 
      tcacgcaat ccacagaac gctcgccca gcaattcaa 
      gtatgcctt agtctgggg cacgagcac caaatactc 
      gccccaaag ggtcggcac ttcccacgt catgcacga 
      cgaggacgt catccaaat agttaagaa gtaattaga 
      ttcgttgtt actgcatat tattaaatg attttctta 
      cac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY