Status
Please wait ...
Gene information
General information
TC005460 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1316392..1318679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005460
    iBeetle screen iB_03956
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005460 RA
      MMDFLNSFK SLVKVEQIR TDNNVFRLH YKLTVIMLI 
      VFSILLTSK QYFGDPINC KVEENRDIV ETYCWIHGT 
      YIRRDTLSG KSGFIPGLG PDNRDIRPW MRSPDDKII 
      WQKYYQWVC IVFCFQALL FYLPRYLWK TWEGGRLRL 
      LVSDLNTPL VTASWNPTT KSQMIQYII NGKYFHTLY 
      AIRYVVCEI LNLANVILQ IFLMDTFLG GQFALYGFK 
      VFANGDINA MNEVFPKLT KCQYRFYGP SGSEVNRDA 
      LCILPLNIL NEKLFIVLW FWLFFLSGV TFLSLIYRF 
      VVVCVPKLR VYLLMAQAR FIGSKQATS IIQKFSYGD 
      FFVLYHVGK NVNPIVFRE LVLGIYETL KDKNPYVYP 
      GVEVNTI
    CDS sequence
    • >TC005460 RA
      atgatggac tttttgaac tctttcaag tccttagta 
      aaggtggaa cagatccgt actgacaat aacgtgttc 
      cgtttgcac tacaaatta accgtgatt atgctgatc 
      gtgttttcc atactcttg accagcaag cagtatttt 
      ggggacccc atcaattgc aaagtggag gagaaccgg 
      gacatcgtc gagacgtac tgctggata cacgggacg 
      tacatccgg cgggacacc ctatcaggc aaaagtggg 
      tttattccg ggtttgggg cccgacaac cgcgacata 
      cggccctgg atgcggagc cctgatgat aaaatcatc 
      tggcagaag tactaccag tgggtttgt atcgttttc 
      tgcttccaa gcgctgctc ttctacctg ccccgttac 
      ttgtggaag acgtgggaa gggggccgt ttgaggctc 
      ctggtgagc gacctcaac acccccctg gtgacggcg 
      tcgtggaac cccaccacc aagtcccaa atgatccag 
      tacataatc aacgggaaa tatttccac accttgtat 
      gccatcaga tacgtcgtc tgtgagata ctcaacttg 
      gccaatgtg attttgcaa attttcctc atggacacg 
      ttcctgggg gggcagttc gccctctac ggttttaag 
      gtctttgcc aacggggac atcaacgca atgaacgaa 
      gtgttcccg aaactgacc aagtgtcag tacaggttt 
      tacggccct tcgggctcc gaggtcaac cgggacgcc 
      ctctgtatc ctcccactg aacatttta aatgaaaag 
      ttgtttatt gtgttgtgg ttttggttg ttttttctt 
      tccggtgtt acttttttg tcgctgatt tatcgtttt 
      gtggtcgtt tgtgtgccg aaactgcgc gtttatctc 
      ctgatggcg caagccagg tttatcggg agtaaacaa 
      gccacttcg attatacaa aagttcagt tacggcgat 
      tttttcgtt ttgtatcat gttggtaaa aatgttaac 
      ccgattgtt ttccgggag ctagtttta ggtatttat 
      gaaacgctc aaggacaaa aatccctac gtgtatccc 
      ggggttgaa gtcaatact atttaa
    mRNA sequence
    • >TC005460 RA
      taacaagcg ttccgacgc caacgacac acaatcaaa 
      agtgttttc catccgtga gcctggcaa cagtacagc 
      tttataaac gccatttgg cggtgcacc acaagcgga 
      caagtgcca cccggacgg gaaaactag caaactgtg 
      tactcacct atccgtaaa agtctttta attaaaaaa 
      ttcgcacaa atatcactc gaaatcaca cagattcag 
      cttgaaatt ctcgtcaaa catctaaat gaccgtcga 
      agcatgacg ccattttta agtctattt ctgttattg 
      ggtcgcgcg catgcgtcg gcccggcga ccgcttccg 
      gccgttcga attttcgcg ccacttgga ggcaaactc 
      ccggaagtt ggcccgtac tagccacgg tactagtac 
      tatgtattt tacgtgccc cactttacc caactacgg 
      tttaattgt ggatttgga aaatcaccg gtggcacac 
      gaggtcgtt tgtaccccc aattaaaca aaaggcgat 
      cggcgcgtt tgaccataa tttaacagt gtcataatt 
      tgatcaccc gaacaataa aaccagcag taaaaacca 
      tttaattgc acaaacaaa aaatctttt tggtggaat 
      aaaaccgcg agttgcgac tttcgcctc tatgcggtt 
      ggcaatagg ggcaggcac ttggcgcca ttttctacc 
      ggtcgagtg tgaaaactt gttcatttt gaaattaac 
      ttacaatga tggactttt tgaactctt tcaagtcct 
      tagtaaagg tggaacaga tccgtactg acaataacg 
      tgttccgtt tgcactaca aattaaccg tgattatgc 
      tgatcgtgt tttccatac tcttgacca gcaagcagt 
      attttgggg accccatca attgcaaag tggaggaga 
      accgggaca tcgtcgaga cgtactgct ggatacacg 
      ggacgtaca tccggcggg acaccctat caggcaaaa 
      gtgggttta ttccgggtt tggggcccg acaaccgcg 
      acatacggc cctggatgc ggagccctg atgataaaa 
      tcatctggc agaagtact accagtggg tttgtatcg 
      ttttctgct tccaagcgc tgctcttct acctgcccc 
      gttacttgt ggaagacgt gggaagggg gccgtttga 
      ggctcctgg tgagcgacc tcaacaccc ccctggtga 
      cggcgtcgt ggaacccca ccaccaagt cccaaatga 
      tccagtaca taatcaacg ggaaatatt tccacacct 
      tgtatgcca tcagatacg tcgtctgtg agatactca 
      acttggcca atgtgattt tgcaaattt tcctcatgg 
      acacgttcc tgggggggc agttcgccc tctacggtt 
      ttaaggtct ttgccaacg gggacatca acgcaatga 
      acgaagtgt tcccgaaac tgaccaagt gtcagtaca 
      ggttttacg gcccttcgg gctccgagg tcaaccggg 
      acgccctct gtatcctcc cactgaaca ttttaaatg 
      aaaagttgt ttattgtgt tgtggtttt ggttgtttt 
      ttctttccg gtgttactt ttttgtcgc tgatttatc 
      gttttgtgg tcgtttgtg tgccgaaac tgcgcgttt 
      atctcctga tggcgcaag ccaggttta tcgggagta 
      aacaagcca cttcgatta tacaaaagt tcagttacg 
      gcgattttt tcgttttgt atcatgttg gtaaaaatg 
      ttaacccga ttgttttcc gggagctag ttttaggta 
      tttatgaaa cgctcaagg acaaaaatc cctacgtgt 
      atcccgggg ttgaagtca atactattt aaagtgttc 
      ctaaacaac ttattactt aagttacca attcctagt 
      taagttaag ctcgttcgt ttggtgtaa ctttaaatc 
      acaaatttt ggacttggg acccaaatt caaacattc 
      tctgaactt atcgttaca tcac