Status
Please wait ...
Gene information
General information
TC005458 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1357465..1362109
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005458
    iBeetle screen iB_03955
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005458 RA
      MSDQGENMV NSESENLCV VCCKTVEIY SVGVCDHPV 
      CFECSTRMR VLCNQNECP ICRGQMPKV IFTDTVAPF 
      ATLFANFQH TNLQDRKFG LIFCTEEIK KAYQRLLEF 
      RCAICTKAN GRRCTFYTF QQLKDHMRR EHNLVYCDL 
      CVENLKIFC FERRCYTKQ ELGYHRRKG DPDNTSHRG 
      HPLCEFCDT RFMDSDELF RHLRRMHLF CHFCDADGK 
      HQYYNSMED LQRHFREEH HLCEEGECS NMPLTAVFR 
      TDIDLKAHI ATEHGRHLS KSANKQART LEFEFTLAP 
      RPGSDRNKR QTRLERTDE EGAVGYDTE PGGPGGGPR 
      TTTLTPQDF PALGNSTVT VQSRPTTTV NFTSKLNPY 
      NSDDFPALG GSRTPAVTI TTDSTARKK GPGVTITRT 
      LRGPSAQNL QMNSENFPR LGAPSNGTS TVHLSLNNV 
      KSSAPNVSI HVNHNGAIT TTHITSTSS HSNGFNINQ 
      RPVEAFPAL GNTSVPLNP KWVQQKPKK PESRLSKVA 
      PAPSLPPSD LTQFPTLKK NEKNAKKSS TVTVPVSGS 
      WPTSASNNK TNKTAKKQK KKSDDETNK NGIVKKRSE 
      LNIGALSLE PSPPPGFPA KPPPGFNVT NQSFPSLGA 
      SNDLTFTSS SGQSYSIKP TNYHQPANF GARNQNLIK 
      RAMNVLDGD TIQEFKSYS AKFREGAMP PEDYHEYCK 
      ALLGPSFKE LFSELLVLL PDIEKQQDL YRVCGPEVR 
      KNLVVCENC SQVVFKREL SEHYNYHAL DSQFPSLGK 
      TPEGGNAWR K
    CDS sequence
    • >TC005458 RA
      atgtcagat cagggcgaa aatatggtc aattccgag 
      tcggaaaac ctgtgcgtg gtgtgttgc aaaacggtg 
      gaaatctac tcggtgggc gtctgcgac caccccgtc 
      tgtttcgag tgctccacc aggatgcga gtcctgtgc 
      aaccagaac gagtgcccc atatgccgg gggcagatg 
      cccaaggtg atcttcacc gacacggtg gcccccttt 
      gccactctc ttcgccaac ttccagcac accaacttg 
      caagaccgc aagttcggc ctgatcttc tgcacggag 
      gagatcaag aaggcgtac cagcggctg ctggagttc 
      cggtgcgcg atctgcacc aaagccaac ggccgcaga 
      tgcacgttc tacaccttc cagcagctg aaggaccac 
      atgagacgc gagcataac ttggtttat tgcgatctg 
      tgtgtggag aatttgaag attttttgc ttcgagcgg 
      cggtgttac accaagcaa gagttgggg taccaccgc 
      aggaaaggc gacccggat aacacgtca cacaggggg 
      cacccgctg tgcgagttt tgtgacacg aggtttatg 
      gatagcgac gaattgttc aggcatttg aggcgaatg 
      cacttgttt tgccacttt tgtgacgct gatggaaaa 
      caccaatat tacaattcg atggaggac ctccagagg 
      catttccgc gaggagcac cacctctgc gaggagggg 
      gagtgcagt aatatgccc ctaacggcc gtttttagg 
      accgacatt gatttaaaa gcccacatt gcaacggaa 
      cacggccgc catttgagc aaatcagcc aataaacaa 
      gcgcgaaca cttgaattt gaatttact ttagcccca 
      agacctggc tctgatcga aacaaacga caaacacgt 
      cttgaacga actgacgaa gaaggtgca gttggctat 
      gacaccgaa cctgggggc cccggaggg ggcccccga 
      actactaca ttaacacca caagatttt ccagctttg 
      ggtaactca actgtgacc gttcaaagt cgccctacg 
      actactgtt aattttacg tcgaaatta aatccttat 
      aacagtgat gattttccg gctttaggt ggttcccgt 
      acgccggcc gtaaccata actacggat tcgacggcg 
      cgtaaaaag ggcccaggg gtgaccata acgcgcact 
      ttgagaggc ccttcggcc caaaatttg caaatgaat 
      tctgagaat ttcccacgt ttaggggcg ccatctaac 
      gggactagc accgtacat ttaagtcta aacaatgtc 
      aaaagctcg gcacccaac gtctcaatc cacgtcaat 
      cataatggt gctataaca accactcac ataacaagc 
      acctcgtca cattctaac ggcttcaac atcaaccaa 
      cgcccagtt gaggctttc cccgcttta ggcaacact 
      tccgtacct ttaaacccg aaatgggtg caacaaaaa 
      ccaaaaaaa ccggaatca cgcttaagt aaagtcgcc 
      ccagcaccg tccctaccc ccgagtgac ttaactcaa 
      ttccccacc ttaaagaaa aacgagaaa aatgccaaa 
      aaatcgtca acagtcacg gtccccgtc tccggctca 
      tggccaact agcgcaagc aataacaaa acaaataaa 
      accgccaag aaacaaaaa aagaaaagc gacgacgaa 
      acgaacaaa aacggcatt gtgaaaaaa cgctccgag 
      ctaaacatc ggcgctttg agtctggag ccgtcgcct 
      ccgccgggc tttcccgcc aagcccccg cccggcttc 
      aacgtgacc aatcagagc ttccccagt ctgggcgcg 
      tccaatgac ttgacgttt acgagcagc agcggccaa 
      tcgtattcg atcaaaccg accaattac caccaaccg 
      gcgaacttt ggggcccgc aaccagaac ctgatcaag 
      cgggccatg aacgtgttg gatggtgat acgattcag 
      gagtttaaa tcgtactcg gccaagttc cgagagggg 
      gcgatgccg cccgaagac taccacgag tactgcaaa 
      gccctgctg gggccaagc tttaaggag ctgttctcc 
      gaactgcta gtccttctg cccgacatc gaaaaacag 
      caagacttg taccgggtc tgtggccca gaggttagg 
      aaaaacctc gtcgtgtgc gaaaactgc agccaggtc 
      gtgtttaaa cgcgaattg agcgagcat tacaattat 
      cacgccttg gacagccag ttccccagt ctggggaaa 
      acccccgag gggggcaat gcctggcgg aagtag
    mRNA sequence
    • >TC005458 RA
      aactgccat cgacctgtc agttacaaa attggttgt 
      gttttcctt gacaaattt ctgattgaa actgtccga 
      aatgtcaga tcagggcga aaatatggt caattccga 
      gtcggaaaa cctgtgcgt ggtgtgttg caaaacggt 
      ggaaatcta ctcggtggg cgtctgcga ccaccccgt 
      ctgtttcga gtgctccac caggatgcg agtcctgtg 
      caaccagaa cgagtgccc catatgccg ggggcagat 
      gcccaaggt gatcttcac cgacacggt ggccccctt 
      tgccactct cttcgccaa cttccagca caccaactt 
      gcaagaccg caagttcgg cctgatctt ctgcacgga 
      ggagatcaa gaaggcgta ccagcggct gctggagtt 
      ccggtgcgc gatctgcac caaagccaa cggccgcag 
      atgcacgtt ctacacctt ccagcagct gaaggacca 
      catgagacg cgagcataa cttggttta ttgcgatct 
      gtgtgtgga gaatttgaa gattttttg cttcgagcg 
      gcggtgtta caccaagca agagttggg gtaccaccg 
      caggaaagg cgacccgga taacacgtc acacagggg 
      gcacccgct gtgcgagtt ttgtgacac gaggtttat 
      ggatagcga cgaattgtt caggcattt gaggcgaat 
      gcacttgtt ttgccactt ttgtgacgc tgatggaaa 
      acaccaata ttacaattc gatggagga cctccagag 
      gcatttccg cgaggagca ccacctctg cgaggaggg 
      ggagtgcag taatatgcc cctaacggc cgtttttag 
      gaccgacat tgatttaaa agcccacat tgcaacgga 
      acacggccg ccatttgag caaatcagc caataaaca 
      agcgcgaac acttgaatt tgaatttac tttagcccc 
      aagacctgg ctctgatcg aaacaaacg acaaacacg 
      tcttgaacg aactgacga agaaggtgc agttggcta 
      tgacaccga acctggggg ccccggagg gggcccccg 
      aactactac attaacacc acaagattt tccagcttt 
      gggtaactc aactgtgac cgttcaaag tcgccctac 
      gactactgt taattttac gtcgaaatt aaatcctta 
      taacagtga tgattttcc ggctttagg tggttcccg 
      tacgccggc cgtaaccat aactacgga ttcgacggc 
      gcgtaaaaa gggcccagg ggtgaccat aacgcgcac 
      tttgagagg cccttcggc ccaaaattt gcaaatgaa 
      ttctgagaa tttcccacg tttaggggc gccatctaa 
      cgggactag caccgtaca tttaagtct aaacaatgt 
      caaaagctc ggcacccaa cgtctcaat ccacgtcaa 
      tcataatgg tgctataac aaccactca cataacaag 
      cacctcgtc acattctaa cggcttcaa catcaacca 
      acgcccagt tgaggcttt ccccgcttt aggcaacac 
      ttccgtacc tttaaaccc gaaatgggt gcaacaaaa 
      accaaaaaa accggaatc acgcttaag taaagtcgc 
      cccagcacc gtccctacc cccgagtga cttaactca 
      attccccac cttaaagaa aaacgagaa aaatgccaa 
      aaaatcgtc aacagtcac ggtccccgt ctccggctc 
      atggccaac tagcgcaag caataacaa aacaaataa 
      aaccgccaa gaaacaaaa aaagaaaag cgacgacga 
      aacgaacaa aaacggcat tgtgaaaaa acgctccga 
      gctaaacat cggcgcttt gagtctgga gccgtcgcc 
      tccgccggg ctttcccgc caagccccc gcccggctt 
      caacgtgac caatcagag cttccccag tctgggcgc 
      gtccaatga cttgacgtt tacgagcag cagcggcca 
      atcgtattc gatcaaacc gaccaatta ccaccaacc 
      ggcgaactt tggggcccg caaccagaa cctgatcaa 
      gcgggccat gaacgtgtt ggatggtga tacgattca 
      ggagtttaa atcgtactc ggccaagtt ccgagaggg 
      ggcgatgcc gcccgaaga ctaccacga gtactgcaa 
      agccctgct ggggccaag ctttaagga gctgttctc 
      cgaactgct agtccttct gcccgacat cgaaaaaca 
      gcaagactt gtaccgggt ctgtggccc agaggttag 
      gaaaaacct cgtcgtgtg cgaaaactg cagccaggt 
      cgtgtttaa acgcgaatt gagcgagca ttacaatta 
      tcacgcctt ggacagcca gttccccag tctggggaa 
      aacccccga ggggggcaa tgcctggcg gaagtagtt 
      ttttggttt gttggagtt ttagtcgct aacttagcc 
      ttatgtgat ttgtttaga actaacaag gtgatcaca 
      gtgtaaata aatagtttt gtatgacga aagtttacg 
      tttgtattt ttaaaataa gaataaagt tgcaataaa 
      tcc