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Gene information
General information
TC005444 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1651799..1655663
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005444
    iBeetle screen iB_03953
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    head completeorientation bent - Number of animals showing the phenotype: 10

    antenna orientation bent - Number of animals showing the phenotype: 10

    Adult morphology
    antenna orientation bent - Number of animals showing the phenotype: 9


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005444 RA
      MNTLTFLLL TFCTSIHTQ TSKFYVIRQ WKYLNFTWP 
      DEDALKTAT ATGDYIPEN NIVSGIKYF EDYYYLTLP 
      RMKKGVPAT LARIKAGPT RDTAPPLEP FPSWGMNQL 
      GDCNNLQNV QNVEIDAKG QVWIIDGGR VQTLVEPVV 
      KCGAKLVVY GLREGRVST VFHFPEEVA ARNGSYLYD 
      LVVDDTDGG FAYITDNSA IDPGIIVFS VKENRAWKI 
      RHSKTMTAD PSARHFTAN GVPISVPIN IAGIALGPK 
      IHTSNEKLI VNEDREVYF SPLSSLHLY SINTSSLRN 
      PANFSDSSV TDLGLKSSQ SVGMVMDNQ GILYYTLLA 
      NNAIGRWDS HTPFQSGQK LIAQDSKYL EWPNSLTLE 
      TMGNLTVLM NRLNQFVYD KFDLNKYNF RLITAFVGG 
      RSYVYDDNF NYDMNRDER MFHNESKTT PEPLPTPAP 
      EPHYDDITS TMSNNVEIV KSASHKLAS SLFTLILCF 
      LLVRR
    CDS sequence
    • >TC005444 RA
      atgaacact ctcaccttc ctcctgctc accttttgc 
      acctcaatc cacactcaa acgagcaaa ttttacgtc 
      atccgccaa tggaagtac ctcaacttc acatggccg 
      gacgaagac gccctcaaa acggccaca gccaccgga 
      gactacatc ccggaaaat aacatcgtt tccgggatc 
      aagtacttc gaggattac tactacttg acgctcccg 
      aggatgaag aagggagta ccggcgacg ctggcacgg 
      atcaaggct gggcccacc cgggacacg gcgccgcct 
      ctggagccc ttcccctcc tgggggatg aaccagctg 
      ggggattgc aacaatttg cagaatgtg caaaacgtg 
      gagattgac gccaagggc caggtgtgg attattgac 
      ggagggagg gtgcagacg ttggtggag ccggtggtt 
      aagtgcggg gccaagttg gtggtgtat gggctgagg 
      gagggaagg gtgagcacg gtgtttcat ttcccggag 
      gaagtcgca gcgagaaat gggagttat ttgtacgat 
      cttgtggtg gatgatact gatggcggg tttgcttat 
      attacggat aatagtgcc atcgatcca ggaataatc 
      gtcttttcg gtgaaagaa aaccgcgcg tggaaaatc 
      cgccactca aaaaccatg acagcagat ccctccgcc 
      cgccatttc accgccaat ggagtgccc atttccgtc 
      cccatcaac atagctggc atagcccta ggccccaaa 
      attcacaca agcaacgaa aaactaata gtcaacgag 
      gacagggag gtttatttc agtccgttg tcaagcctc 
      cacctttac tcaatcaac acttcgagt ctccgaaac 
      cctgctaat ttctccgac agctccgtc accgacttg 
      gggctgaaa tcctcacag tcggtgggg atggtgatg 
      gacaaccaa gggatactt tattacacc cttttggcc 
      aacaacgcc atagggcga tgggacagt cacaccccg 
      ttccaatcc gggcagaaa ttaatcgcc caggacagc 
      aaataccta gagtggccc aactctcta actttggaa 
      actatgggc aacttgacc gtgcttatg aatcggctc 
      aaccaattc gtttacgat aagttcgac ttaaacaag 
      tacaatttt agactgatt acggccttt gtgggcggg 
      agaagttac gtgtacgac gataatttc aattatgat 
      atgaatagg gacgaaagg atgtttcac aatgaaagt 
      aaaacgact ccagaacca cttccaact cctgcaccg 
      gagccgcat tacgatgac ataacatca acgatgagc 
      aataacgtg gaaatagtt aaatcagca agtcataaa 
      ttagcgagt agtttgttt actttgatt ttgtgtttt 
      ctgctagtg cgacggtga
    mRNA sequence
    • >TC005444 RA
      tgaacttga ccactcgca atgaacact ctcaccttc 
      ctcctgctc accttttgc acctcaatc cacactcaa 
      acgagcaaa ttttacgtc atccgccaa tggaagtac 
      ctcaacttc acatggccg gacgaagac gccctcaaa 
      acggccaca gccaccgga gactacatc ccggaaaat 
      aacatcgtt tccgggatc aagtacttc gaggattac 
      tactacttg acgctcccg aggatgaag aagggagta 
      ccggcgacg ctggcacgg atcaaggct gggcccacc 
      cgggacacg gcgccgcct ctggagccc ttcccctcc 
      tgggggatg aaccagctg ggggattgc aacaatttg 
      cagaatgtg caaaacgtg gagattgac gccaagggc 
      caggtgtgg attattgac ggagggagg gtgcagacg 
      ttggtggag ccggtggtt aagtgcggg gccaagttg 
      gtggtgtat gggctgagg gagggaagg gtgagcacg 
      gtgtttcat ttcccggag gaagtcgca gcgagaaat 
      gggagttat ttgtacgat cttgtggtg gatgatact 
      gatggcggg tttgcttat attacggat aatagtgcc 
      atcgatcca ggaataatc gtcttttcg gtgaaagaa 
      aaccgcgcg tggaaaatc cgccactca aaaaccatg 
      acagcagat ccctccgcc cgccatttc accgccaat 
      ggagtgccc atttccgtc cccatcaac atagctggc 
      atagcccta ggccccaaa attcacaca agcaacgaa 
      aaactaata gtcaacgag gacagggag gtttatttc 
      agtccgttg tcaagcctc cacctttac tcaatcaac 
      acttcgagt ctccgaaac cctgctaat ttctccgac 
      agctccgtc accgacttg gggctgaaa tcctcacag 
      tcggtgggg atggtgatg gacaaccaa gggatactt 
      tattacacc cttttggcc aacaacgcc atagggcga 
      tgggacagt cacaccccg ttccaatcc gggcagaaa 
      ttaatcgcc caggacagc aaataccta gagtggccc 
      aactctcta actttggaa actatgggc aacttgacc 
      gtgcttatg aatcggctc aaccaattc gtttacgat 
      aagttcgac ttaaacaag tacaatttt agactgatt 
      acggccttt gtgggcggg agaagttac gtgtacgac 
      gataatttc aattatgat atgaatagg gacgaaagg 
      atgtttcac aatgaaagt aaaacgact ccagaacca 
      cttccaact cctgcaccg gagccgcat tacgatgac 
      ataacatca acgatgagc aataacgtg gaaatagtt 
      aaatcagca agtcataaa ttagcgagt agtttgttt 
      actttgatt ttgtgtttt ctgctagtg cgacggtga 
      aacaacgag aaatttcgg tcgggtcgc tcaaaatgg 
      cccccggac ggccatttt gagcctgta aatatactc 
      tgtttagga tagtcatta gcttgatca gactgtttt 
      gttttgtaa tcattatta attctgttg cgttgttgt 
      ctagtgttt tagttacca aagccatta acacgtaat 
      aaaatatcg aaaacaaa