Status
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Gene information
General information
TC034169
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005439,TC005440,TC005441
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid57: 1710034..1716197
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034169
    iBeetle screen iB_03951
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034169 RA
      MSEVKVKIQ DEPPKPRKL IGVRHLQFV LLFLSLLIY 
      FGMRISLSV AIISMTDDQ PPDSTIPTY PEWDNTDTI 
      LSSFFWSYT IPQVFAGQL SEYFGPKWF LVSTMVLTS 
      LVNIAIPPM AALMGSTGV IICRLLQGL LQSVLYPSV 
      HHLISRWSP LYERSRVAN FVYGGEVLG TAVSMLLTG 
      VICGSSWGW PGSFYAYGG VGVGFAMVF AYLAENSPS 
      CHKGITEEE RDYIESSNS VNVEKKIPT PWWPLVTSL 
      PVWAIIVAG IAQCFGSFA LVTEIPGYM DNIMNYDIN 
      SNSQLSALP YVIQFFLGL AVSPLADLI AKRKLVTIS 
      TSRKIFNSL ASVLPASGL IYLAFLTNY DKTLVTVLL 
      IIAISTSTF ASSGYLINV VDVAPNHSG TLFGIVNGI 
      NNVFSMLAP LSVSFFGTN KISETPPKP KNLIGVRHF 
      QFVLMFFSI LISFAIRTS LSVSIISMT AKTPPDPSI 
      PTYPGWNNV DLILSSFFW GYIIPQIGA GQLSEFFGP 
      KWFLVGTMI IGSLANIAI PPLAATLGP SGVITCRVI 
      QGLTQGFLY PSILNLISR WAPLFERAR ISNFVYGSN 
      SLGMAIAMP LTGVIAGSD LGWPVAFYI FGGLGIIWA 
      LIFAVFAQN SPSDHGGIS QEEKEYIMR SNFVTTEKK 
      KVPTPWSKI ATSRPVWAV LLASIAQCF GYFTLMAEM 
      PTYMSHMLK FDINSHSEL AALPYLGNF FLGLFVSPL 
      ADLLVTKKI CSISTVRKI FNGLAAFVP GAALTYLAF 
      LENFTEALV TTFLVIACS FSSFIYSGY IVNIIDFAP 
      NHSGTIMGM ANCIGNVFS ILGPITVGL FGEDKNDPL 
      LWRKVFLLA AGIYGGCGL FYVVFASAE VQSWNEPED 
      EEKNENHST KLPIENAKP TPKHFFGVR HVQIGLMFF 
      SIIIFYAQR TSMSVAIIA MTDETPPSP NIPTYPKWD 
      NTDIILSSF FWGYIVPQV GAGQLGEYF GPKWFLFTT 
      MTIGSIFNI LVPTMAEVM GPTGVIICR VVQGLNQGF 
      LYPSTNNLI SKWTPLYER SRVSSFVFG GSNVGIILS 
      MIFTGALSG SSWGWPSAF YIYGAVGIL WAIIFVIFV 
      ANSPALHPS ISDEEREYI ESNNSCDSG EKKKVPTPW 
      KLIARSLPV WAVLVTSCG NSWGGFTLL TEIPSYMSN 
      IMGFDINSN SQLSALPYL AMFIVAMGS APIADKLIS 
      NKILTIGTT RKIFNSMGC LIPAAALFS LGLVDSSQK 
      DLGTVLLVI AVGACGFVY SGALVNLVD LAPNHAGTL 
      LGITNGTST IFSILGPLS VQFLGSDKK DPILWRKVF 
      WLTAGIYVG CGLFYAIFC SGELQEWNG EEDKVDRKK 
      SESSTNKEK SPV
    CDS sequence
    • >TC034169 RA
      atgtcagaa gttaaagta aaaatacaa gacgaacca 
      ccaaaaccc cggaaacta atcggcgta cggcacttg 
      cagttcgtt ttactgttc ctttcactc ttgatttat 
      ttcgggatg cgaatctcc ctctccgtt gccataatt 
      tcaatgacc gacgaccaa cctccagac tccacaatt 
      cccacgtac ccagaatgg gacaacacc gacacaatc 
      ctctcgtct tttttctgg agttacacc atcccccaa 
      gtcttcgcc ggccaactg agcgagtac ttcggcccc 
      aagtggttc ctggtcagt accatggtc ctgacctcg 
      ctggtcaac atcgccatc ccgcccatg gctgccctg 
      atgggctcc actggggtc ataatctgc cggttactt 
      caaggtctc ctccagagt gttctgtac ccttccgtt 
      caccatttg atcagccgg tggtcgcct ctttatgag 
      cggtcccgc gttgctaat ttcgtgtac gggggtgag 
      gttttgggg acggcagtc tccatgtta ctgacaggg 
      gtgatttgc gggtctagc tgggggtgg cctgggtcg 
      ttttatgcc tatgggggt gttggggtg ggttttgcg 
      atggttttt gcatacttg gccgaaaat tcgccctct 
      tgtcacaag gggataacg gaggaggag cgtgattac 
      atagagagt agcaattcg gtcaatgtc gaaaagaaa 
      ataccaaca ccatggtgg ccccttgtc acttcttta 
      ccagtgtgg gcgattatc gtggctggg attgcccag 
      tgttttggt agttttgct ctagtgacc gaaataccg 
      ggctatatg gacaatatc atgaattat gatatcaat 
      tcaaacagc caactgtcg gcactccct tacgttatc 
      cagtttttc ctaggcctg gctgtttcc cctctggcc 
      gacttgatc gcgaaacga aaattagta acaatcagt 
      acttcccga aaaattttc aattccctg gcgtccgtt 
      ttaccagcc tcagggtta atttatttg gcctttttg 
      accaattat gataaaact ctagtcacg gtactgctt 
      attatagcc ataagtact tcgactttt gccagttct 
      ggttatttg atcaatgtg gtcgacgtg gcaccgaat 
      cattctgga actcttttt gggattgtc aatgggatt 
      aacaacgtt ttttccatg ttggcaccg ctgagtgtg 
      tcttttttt ggcactaat aagatcagt gaaactccc 
      ccaaaacca aaaaatctc atcggtgtc agacacttc 
      cagttcgtg ttaatgttc ttctcaatt ttgatttca 
      ttcgctata cgaacgtcg ctatcagtc tcaattatt 
      tcaatgacg gccaagaca ccgcccgac cctagcata 
      ccgacatac cctgggtgg aacaacgtt gatttaatc 
      ctctcctca tttttctgg ggctacata atcccacag 
      attggtgcc ggccaacta agcgagttt ttcggccct 
      aagtggttc ctggtcggt accatgatc attggttcc 
      cttgccaac atcgccata ccgcctcta gcggcaact 
      ttgggccct tctggggtg ataacatgt cgtgtcatc 
      cagggccta actcagggt ttcctctac ccttcaatc 
      ctcaatctc ataagccga tgggcgccc ttattcgaa 
      agggcccga atttcgaat tttgtctac gggagtaat 
      agtctaggg atggcaatt gctatgccc ttgaccggt 
      gttatagcc ggttcggat ttagggtgg cctgtagct 
      ttctacatt ttcggcggt ttggggata atttgggcc 
      cttattttt gcggttttt gcccaaaac tcaccctca 
      gaccatggg gggatttcg caagaggag aaagaatac 
      ataatgcgg agcaatttt gtcacaaca gaaaagaaa 
      aaagtgcca acgccgtgg tcaaaaatt gcgacttcg 
      cgcccagtg tgggccgtt ttattggcg tccattgcc 
      cagtgcttt ggttatttc accctaatg gccgaaatg 
      cccacttat atgagccac atgttgaag tttgacatc 
      aattctcac agtgaattg gcagcgttg ccctatttg 
      ggcaacttt tttttgggc ctttttgtc tcacctttg 
      gccgatttg ctcgtaacc aagaaaatc tgttcgatt 
      agcacagtt aggaaaatt tttaatggt ttggcggct 
      tttgtgcca ggtgcggct ttaacttac ctggctttt 
      ctcgaaaat tttaccgaa gctctagtc accacgttt 
      ctggtcatt gcgtgtagt ttttcgtct tttatttat 
      tcaggttat attgttaat attattgac tttgcccca 
      aaccattct gggaccatt atgggcatg gctaactgc 
      atcgggaat gttttttcg attttgggg cccataacg 
      gtgggactt tttggcgag gataaaaat gacccgctt 
      ttgtggagg aaggttttt cttctagcg gctggaatt 
      tacggcggt tgtggactg ttttatgtc gtttttgcc 
      tctgcggaa gtacaaagt tggaacgag ccagaggat 
      gaagaaaaa aacgaaaat cattccacg aaacttcca 
      attgaaaat gccaaaccc acaccaaag catttcttt 
      ggcgtacga cacgtccaa atcgggttg atgttcttc 
      tcgattata attttttac gcacagagg acgtccatg 
      tccgttgct atcatcgca atgaccgac gagacgccc 
      ccaagccct aacataccc acgtaccca aaatgggac 
      aatactgac ataattttg tcgtcattt ttctggggg 
      tacattgtc ccacaagtc ggtgccggc caactgggc 
      gaatatttc ggccccaaa tggttcctc ttcaccacc 
      atgaccatt ggttccatt ttcaatatt ttggtacca 
      acaatggcc gaagttatg ggacccact ggggttata 
      atatgtcgg gtggtgcaa ggcttgaac caaggtttt 
      ctctaccca tccacaaac aatttgatt agtaaatgg 
      accccattg tacgaacgg tcacgagtt tcaagtttt 
      gtgttcggt ggttcaaat gttggtatt atcttgtct 
      atgattttt acgggggcc ctttcgggc tctagctgg 
      ggttggcct tcagcgttt tacatttat ggggctgtt 
      ggtatttta tgggccatt attttcgta attttcgtg 
      gccaattcc ccagcgctt cacccaagt atttcagat 
      gaggaaaga gaatacatc gaaagtaac aattcgtgt 
      gatagtggt gaaaaaaag aaagtacca acaccttgg 
      aaactaatc gcaaggtcg ctcccagtt tgggccgtt 
      ttggtcacg tcctgtggg aacagctgg ggtggtttc 
      accctcctg acggaaatt cccagctac atgagcaac 
      atcatgggc tttgatatc aattcaaat agtcaacta 
      tcggccctc ccgtacctt gccatgttc atagtggcc 
      atgggcagt gccccaatt gccgacaaa ttaatttcg 
      aataaaatt ctcacgatt ggaacaact cgaaaaatt 
      ttcaattct atgggttgt ctaatccca gcagctgcc 
      ttattcagc cttggtctg gtcgacagt tcgcagaaa 
      gacttagga acagttttg ctagttatt gctgttggt 
      gcttgtggt tttgtctat tctggagct ttggttaat 
      ttggtagac ttggcccca aaccatgct ggtactttg 
      ttgggtatt accaacggt acgtccacg attttttcg 
      attttgggg cctttatca gtccagttc ctcggaagt 
      gataagaaa gacccgatt ttgtggagg aaggtgttt 
      tggctaact gctgggatt tacgtcggt tgtggtttg 
      ttctacgcc atattttgt tcaggggag ttgcaagag 
      tggaatggg gaggaagac aaagtggac aggaaaaaa 
      tctgaaagt agtacaaat aaagaaaaa agccctgtt 
      tag
    mRNA sequence
    • >TC034169 RA
      aaaatttgg agattatca gtttcgcaa aatttaacc 
      cgtcgaaca aaaatgtca gaagttaaa gtaaaaata 
      caagacgaa ccaccaaaa ccccggaaa ctaatcggc 
      gtacggcac ttgcagttc gttttactg ttcctttca 
      ctcttgatt tatttcggg atgcgaatc tccctctcc 
      gttgccata atttcaatg accgacgac caacctcca 
      gactccaca attcccacg tacccagaa tgggacaac 
      accgacaca atcctctcg tcttttttc tggagttac 
      accatcccc caagtcttc gccggccaa ctgagcgag 
      tacttcggc cccaagtgg ttcctggtc agtaccatg 
      gtcctgacc tcgctggtc aacatcgcc atcccgccc 
      atggctgcc ctgatgggc tccactggg gtcataatc 
      tgccggtta cttcaaggt ctcctccag agtgttctg 
      tacccttcc gttcaccat ttgatcagc cggtggtcg 
      cctctttat gagcggtcc cgcgttgct aatttcgtg 
      tacgggggt gaggttttg gggacggca gtctccatg 
      ttactgaca ggggtgatt tgcgggtct agctggggg 
      tggcctggg tcgttttat gcctatggg ggtgttggg 
      gtgggtttt gcgatggtt tttgcatac ttggccgaa 
      aattcgccc tcttgtcac aaggggata acggaggag 
      gagcgtgat tacatagag agtagcaat tcggtcaat 
      gtcgaaaag aaaatacca acaccatgg tggcccctt 
      gtcacttct ttaccagtg tgggcgatt atcgtggct 
      gggattgcc cagtgtttt ggtagtttt gctctagtg 
      accgaaata ccgggctat atggacaat atcatgaat 
      tatgatatc aattcaaac agccaactg tcggcactc 
      ccttacgtt atccagttt ttcctaggc ctggctgtt 
      tcccctctg gccgacttg atcgcgaaa cgaaaatta 
      gtaacaatc agtacttcc cgaaaaatt ttcaattcc 
      ctggcgtcc gttttacca gcctcaggg ttaatttat 
      ttggccttt ttgaccaat tatgataaa actctagtc 
      acggtactg cttattata gccataagt acttcgact 
      tttgccagt tctggttat ttgatcaat gtggtcgac 
      gtggcaccg aatcattct ggaactctt tttgggatt 
      gtcaatggg attaacaac gttttttcc atgttggca 
      ccgctgagt gtgtctttt tttggcact aataagatc 
      agtgaaact cccccaaaa ccaaaaaat ctcatcggt 
      gtcagacac ttccagttc gtgttaatg ttcttctca 
      attttgatt tcattcgct atacgaacg tcgctatca 
      gtctcaatt atttcaatg acggccaag acaccgccc 
      gaccctagc ataccgaca taccctggg tggaacaac 
      gttgattta atcctctcc tcatttttc tggggctac 
      ataatccca cagattggt gccggccaa ctaagcgag 
      tttttcggc cctaagtgg ttcctggtc ggtaccatg 
      atcattggt tcccttgcc aacatcgcc ataccgcct 
      ctagcggca actttgggc ccttctggg gtgataaca 
      tgtcgtgtc atccagggc ctaactcag ggtttcctc 
      tacccttca atcctcaat ctcataagc cgatgggcg 
      cccttattc gaaagggcc cgaatttcg aattttgtc 
      tacgggagt aatagtcta gggatggca attgctatg 
      cccttgacc ggtgttata gccggttcg gatttaggg 
      tggcctgta gctttctac attttcggc ggtttgggg 
      ataatttgg gcccttatt tttgcggtt tttgcccaa 
      aactcaccc tcagaccat ggggggatt tcgcaagag 
      gagaaagaa tacataatg cggagcaat tttgtcaca 
      acagaaaag aaaaaagtg ccaacgccg tggtcaaaa 
      attgcgact tcgcgccca gtgtgggcc gttttattg 
      gcgtccatt gcccagtgc tttggttat ttcacccta 
      atggccgaa atgcccact tatatgagc cacatgttg 
      aagtttgac atcaattct cacagtgaa ttggcagcg 
      ttgccctat ttgggcaac ttttttttg ggccttttt 
      gtctcacct ttggccgat ttgctcgta accaagaaa 
      atctgttcg attagcaca gttaggaaa atttttaat 
      ggtttggcg gcttttgtg ccaggtgcg gctttaact 
      tacctggct tttctcgaa aattttacc gaagctcta 
      gtcaccacg tttctggtc attgcgtgt agtttttcg 
      tcttttatt tattcaggt tatattgtt aatattatt 
      gactttgcc ccaaaccat tctgggacc attatgggc 
      atggctaac tgcatcggg aatgttttt tcgattttg 
      gggcccata acggtggga ctttttggc gaggataaa 
      aatgacccg cttttgtgg aggaaggtt tttcttcta 
      gcggctgga atttacggc ggttgtgga ctgttttat 
      gtcgttttt gcctctgcg gaagtacaa agttggaac 
      gagccagag gatgaagaa aaaaacgaa aatcattcc 
      acgaaactt ccaattgaa aatgccaaa cccacacca 
      aagcatttc tttggcgta cgacacgtc caaatcggg 
      ttgatgttc ttctcgatt ataattttt tacgcacag 
      aggacgtcc atgtccgtt gctatcatc gcaatgacc 
      gacgagacg cccccaagc cctaacata cccacgtac 
      ccaaaatgg gacaatact gacataatt ttgtcgtca 
      tttttctgg gggtacatt gtcccacaa gtcggtgcc 
      ggccaactg ggcgaatat ttcggcccc aaatggttc 
      ctcttcacc accatgacc attggttcc attttcaat 
      attttggta ccaacaatg gccgaagtt atgggaccc 
      actggggtt ataatatgt cgggtggtg caaggcttg 
      aaccaaggt tttctctac ccatccaca aacaatttg 
      attagtaaa tggacccca ttgtacgaa cggtcacga 
      gtttcaagt tttgtgttc ggtggttca aatgttggt 
      attatcttg tctatgatt tttacgggg gccctttcg 
      ggctctagc tggggttgg ccttcagcg ttttacatt 
      tatggggct gttggtatt ttatgggcc attattttc 
      gtaattttc gtggccaat tccccagcg cttcaccca 
      agtatttca gatgaggaa agagaatac atcgaaagt 
      aacaattcg tgtgatagt ggtgaaaaa aagaaagta 
      ccaacacct tggaaacta atcgcaagg tcgctccca 
      gtttgggcc gttttggtc acgtcctgt gggaacagc 
      tggggtggt ttcaccctc ctgacggaa attcccagc 
      tacatgagc aacatcatg ggctttgat atcaattca 
      aatagtcaa ctatcggcc ctcccgtac cttgccatg 
      ttcatagtg gccatgggc agtgcccca attgccgac 
      aaattaatt tcgaataaa attctcacg attggaaca 
      actcgaaaa attttcaat tctatgggt tgtctaatc 
      ccagcagct gccttattc agccttggt ctggtcgac 
      agttcgcag aaagactta ggaacagtt ttgctagtt 
      attgctgtt ggtgcttgt ggttttgtc tattctgga 
      gctttggtt aatttggta gacttggcc ccaaaccat 
      gctggtact ttgttgggt attaccaac ggtacgtcc 
      acgattttt tcgattttg gggccttta tcagtccag 
      ttcctcgga agtgataag aaagacccg attttgtgg 
      aggaaggtg ttttggcta actgctggg atttacgtc 
      ggttgtggt ttgttctac gccatattt tgttcaggg 
      gagttgcaa gagtggaat ggggaggaa gacaaagtg 
      gacaggaaa aaatctgaa agtagtaca aataaagaa 
      aaaagccct gtttagttt ttatcacag tgataaagt 
      taagaacaa gtgacatta tagtaatgt tttatttat 
      tttttgtaa taaaataat acaaaatca