| General information | |||
| TC034173
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 13455725..13483321 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034173 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03947 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
| Comment: No phenotype found!!! |
- >TC034173 RA
MATFNNFPE EGRPPDSPS RKFTRKHYP PRNNSWSHP NSLICAENA EINRNVGDK KSHSLKNKL FCIKKPLDE DKTTLNILN VQTLGDSSA SKAVCDNDP SCSRDRNRN SFHQDVEEA LSSLLWQPY EYQNCSSCS SSVTLSDLD ESSPNKGPL HSSDLNLRT QMVNNLKPS PSQSRPGKP SRDAFSCYA ASRLNSQAS LLELLDGGT GDSVSDCDV LPCKNSDMH LEVTEPDPG ATTNVLSIP TGTHGLSSA NVVGLPENP RRASVPSRS VHPRNGSEP VRTQDPITH LRSASVPKQ MPNVSNINV NFYRAVPVN VVSSVPTIQ CLNTLEGNN VSNVQIMPL GSGTTQVTV HNPTSVNVV RTFTSTEAQ TDEIAVCPP PPETTATTR EQRRKERRE RRHQRRTNG TNHRHTVDS GTQANTLQN ERLPDLLNS HMPPPYSPL PSNVPPPNV VLPTPMMVP PPHLQSVGP NNVVPSGLV AFPPPQVVA GQGPVAVPV PPPSGFRFP FPAGGFRRG RFSEEPPKG CCGLLAWKP GSLRWFIAV IALVAVCCV LVGTALGAM RPAGRDHLT VSLLMIGVG IVLVTVSGV AWRLTSHDS STCRSMLGL GSTESVDVC TRRFVPRLP PSYGRPHHP YAAMMYPEF QYRPPPPSY QASMQEYRL RLLLLDRGN TPQIQAGIQ NAVSPPPTY RSHAGSLLR APLSNRRDT AQSEYSCPP SYRSQNSTN RPGTLQNSV LHSREQSLS LSESNQEES VNVVNILGS TEEDMALDN ITLDSLKME PESDINPLR MLLKGSSSD LEGSKDGNL VTIVQTSDQ SPVIVTVSG SSQIEGSTM QIPSEMEIL AHL
- >TC034173 RA
atggccact ttcaataat ttcccggaa gagggtcgt ccgccggac tcgccgtcc cggaagttc acccggaaa cattacccg ccgcggaat aactcctgg agtcacccc aatagctta atctgtgcg gaaaatgcc gaaatcaat cgtaacgtg ggcgataaa aagagccac agtctcaaa aacaagcta ttttgtatc aaaaagccc ctcgatgag gataaaacc acgttgaat atcctcaat gtccagaca ttgggcgat tcaagtgcc tctaaagcc gtttgtgat aatgacccg tcctgttcc cgtgatcgg aacaggaat tcgttccac caggacgtg gaggaggct ctgagttcg ctgttgtgg caaccgtac gagtaccag aactgcagc tcttgcagc agtagtgta acgttaagc gaccttgat gagtcgtcg ccaaacaaa ggcccttta cactcatca gacctaaat cttcgaacc caaatggtg aacaacctg aagccgtca cccagccaa tcgcggccc ggcaagccg tcacgtgac gcgttctct tgttatgct gcaagtagg ttgaacagt caagccagt ttactggag ttactcgac ggtggtacc ggtgatagt gtcagtgat tgtgatgtg ttgccctgc aaaaacagt gatatgcac ctggaagtc acggaaccc gaccccggt gccaccacc aacgtgctg agcatcccg acgggaacc catggcctg agcagcgcc aatgtcgtg ggtctgccc gagaacccg cgacgggcg tcggtacca tcgcggagc gtccatccg cgcaacggc tcggaaccc gtccgcacc caggacccc ataacccac ctccggagc gcctccgtg cccaagcaa atgcccaac gttagcaac atcaacgtg aatttctac cgagcggtt ccagtcaac gtcgtatct tcggtacca accatccaa tgcttgaat accctcgaa gggaacaat gtttccaac gtgcagatc atgccgctg ggctcggga accactcaa gtgacggtc cacaacccc acttccgtg aacgtggtg cgaaccttc acctccacg gaggcgcag acggacgag atcgccgtt tgtccgccg ccgccggag acgacggcc accaccagg gagcagaga cggaaggag cggcgggag cggcgccac caaagacga accaacggt accaatcat cgacatacg gtcgacagt ggtacccaa gccaatact ttgcaaaat gaaaggctt ccggatttg ctcaatagt cacatgccg ccgccgtat tcaccccta cctagtaat gtgccgccc cctaatgtg gtactaccg actcctatg atggtacca ccaccgcat cttcaaagt gttggacct aacaatgta gtgcctagt ggtttagtg gcgtttccg cccccgcaa gttgtggcg gggcaagga ccggtggcg gttccagtg ccgccgcct tcagggttt aggtttccg ttcccagcc gggggattc agaagaggc cgcttctcc gaggagccc cccaaaggc tgttgcggg ctgttggcg tggaaaccg ggctctttg cgctggttc atagcagtg atcgctttg gtggctgtc tgttgtgtg ttggttgga acagccctg ggtgccatg agacccgca ggacgggac catctcaca gtgtccctc ctcatgata ggtgtgggc atcgtcttg gtgacggtc agtggcgtg gcctggaga ctcacttcg cacgattcg tcaacttgt cgctcgatg ctcggctta ggttcgacc gaatcggtc gatgtgtgc actcgacgt ttcgttcct cgtttaccg ccgtcgtac ggccgtccc catcacccc tacgccgct atgatgtac ccagagttc cagtaccgg ccgccgccc cctagctac caagcctcc atgcaagaa tatagactg agacttttg cttctggac agaggcaac actccgcaa atccaggcc gggatacag aacgccgtc tcgccgcct ccgacgtac cgcagccac gctggcagt ctgcttagg gcgcctttg tcgaatcgg agagacacg gcacagtcg gagtacagt tgtcctccg tcttatcgt tcgcaaaac agtacgaat cgaccgggg actctacaa aattcggtt ctgcacagc cgggaacag agcttgagt ttgtcggag tcgaatcag gaagagtcg gtcaacgtt gtgaatatt ttaggcagt actgaagaa gacatggct ttagataat ataacttta gattcgttg aaaatggag ccggaaagt gatattaat ccgttgagg atgcttctt aaaggcagt tccagtgat cttgaaggt agcaaagac gggaatttg gtcactatt gtgcaaacg agcgaccaa agcccggtc attgttact gtgagcgga agctcgcag atcgaaggc agtacaatg caaatacca tccgaaatg gagatactg gcgcactta tag
- >TC034173 RA
gatatttgc cttatgcaa atggcgaat cgttccgtt gaactatga ccgttccga tgaaaaact atggccact ttcaataat ttcccggaa gagggtcgt ccgccggac tcgccgtcc cggaagttc acccggaaa cattacccg ccgcggaat aactcctgg agtcacccc aatagctta atctgtgcg gaaaatgcc gaaatcaat cgtaacgtg ggcgataaa aagagccac agtctcaaa aacaagcta ttttgtatc aaaaagccc ctcgatgag gataaaacc acgttgaat atcctcaat gtccagaca ttgggcgat tcaagtgcc tctaaagcc gtttgtgat aatgacccg tcctgttcc cgtgatcgg aacaggaat tcgttccac caggacgtg gaggaggct ctgagttcg ctgttgtgg caaccgtac gagtaccag aactgcagc tcttgcagc agtagtgta acgttaagc gaccttgat gagtcgtcg ccaaacaaa ggcccttta cactcatca gacctaaat cttcgaacc caaatggtg aacaacctg aagccgtca cccagccaa tcgcggccc ggcaagccg tcacgtgac gcgttctct tgttatgct gcaagtagg ttgaacagt caagccagt ttactggag ttactcgac ggtggtacc ggtgatagt gtcagtgat tgtgatgtg ttgccctgc aaaaacagt gatatgcac ctggaagtc acggaaccc gaccccggt gccaccacc aacgtgctg agcatcccg acgggaacc catggcctg agcagcgcc aatgtcgtg ggtctgccc gagaacccg cgacgggcg tcggtacca tcgcggagc gtccatccg cgcaacggc tcggaaccc gtccgcacc caggacccc ataacccac ctccggagc gcctccgtg cccaagcaa atgcccaac gttagcaac atcaacgtg aatttctac cgagcggtt ccagtcaac gtcgtatct tcggtacca accatccaa tgcttgaat accctcgaa gggaacaat gtttccaac gtgcagatc atgccgctg ggctcggga accactcaa gtgacggtc cacaacccc acttccgtg aacgtggtg cgaaccttc acctccacg gaggcgcag acggacgag atcgccgtt tgtccgccg ccgccggag acgacggcc accaccagg gagcagaga cggaaggag cggcgggag cggcgccac caaagacga accaacggt accaatcat cgacatacg gtcgacagt ggtacccaa gccaatact ttgcaaaat gaaaggctt ccggatttg ctcaatagt cacatgccg ccgccgtat tcaccccta cctagtaat gtgccgccc cctaatgtg gtactaccg actcctatg atggtacca ccaccgcat cttcaaagt gttggacct aacaatgta gtgcctagt ggtttagtg gcgtttccg cccccgcaa gttgtggcg gggcaagga ccggtggcg gttccagtg ccgccgcct tcagggttt aggtttccg ttcccagcc gggggattc agaagaggc cgcttctcc gaggagccc cccaaaggc tgttgcggg ctgttggcg tggaaaccg ggctctttg cgctggttc atagcagtg atcgctttg gtggctgtc tgttgtgtg ttggttgga acagccctg ggtgccatg agacccgca ggacgggac catctcaca gtgtccctc ctcatgata ggtgtgggc atcgtcttg gtgacggtc agtggcgtg gcctggaga ctcacttcg cacgattcg tcaacttgt cgctcgatg ctcggctta ggttcgacc gaatcggtc gatgtgtgc actcgacgt ttcgttcct cgtttaccg ccgtcgtac ggccgtccc catcacccc tacgccgct atgatgtac ccagagttc cagtaccgg ccgccgccc cctagctac caagcctcc atgcaagaa tatagactg agacttttg cttctggac agaggcaac actccgcaa atccaggcc gggatacag aacgccgtc tcgccgcct ccgacgtac cgcagccac gctggcagt ctgcttagg gcgcctttg tcgaatcgg agagacacg gcacagtcg gagtacagt tgtcctccg tcttatcgt tcgcaaaac agtacgaat cgaccgggg actctacaa aattcggtt ctgcacagc cgggaacag agcttgagt ttgtcggag tcgaatcag gaagagtcg gtcaacgtt gtgaatatt ttaggcagt actgaagaa gacatggct ttagataat ataacttta gattcgttg aaaatggag ccggaaagt gatattaat ccgttgagg atgcttctt aaaggcagt tccagtgat cttgaaggt agcaaagac gggaatttg gtcactatt gtgcaaacg agcgaccaa agcccggtc attgttact gtgagcgga agctcgcag atcgaaggc agtacaatg caaatacca tccgaaatg gagatactg gcgcactta tagtcgaaa ctcccattc cttaaggtt agtgtgcag taatgtatc acgttgtat tataattat gcgatgaca tgtgcctat attgtgtag atagtttat aaatagcac tctgtatat tactattat attttaaac tgtataaaa gtaaatttt taataagaa ttgttaaac cgtctcttc attcaaaac caccctcct tttatcctt aaatggacc gacattttt ttaataaat tctccccca tatg
