Status
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Gene information
General information
TC034173
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005419,TC005418 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13455725..13483321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034173
    iBeetle screen iB_03947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 2

    Fertility
    egg production: number <50% of expected -
    Comment: No phenotype found!!!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034173 RA
      MATFNNFPE EGRPPDSPS RKFTRKHYP PRNNSWSHP 
NSLICAENA EINRNVGDK KSHSLKNKL FCIKKPLDE 
DKTTLNILN VQTLGDSSA SKAVCDNDP SCSRDRNRN 
SFHQDVEEA LSSLLWQPY EYQNCSSCS SSVTLSDLD 
ESSPNKGPL HSSDLNLRT QMVNNLKPS PSQSRPGKP 
SRDAFSCYA ASRLNSQAS LLELLDGGT GDSVSDCDV 
LPCKNSDMH LEVTEPDPG ATTNVLSIP TGTHGLSSA 
NVVGLPENP RRASVPSRS VHPRNGSEP VRTQDPITH 
LRSASVPKQ MPNVSNINV NFYRAVPVN VVSSVPTIQ 
CLNTLEGNN VSNVQIMPL GSGTTQVTV HNPTSVNVV 
RTFTSTEAQ TDEIAVCPP PPETTATTR EQRRKERRE 
RRHQRRTNG TNHRHTVDS GTQANTLQN ERLPDLLNS 
HMPPPYSPL PSNVPPPNV VLPTPMMVP PPHLQSVGP 
NNVVPSGLV AFPPPQVVA GQGPVAVPV PPPSGFRFP 
FPAGGFRRG RFSEEPPKG CCGLLAWKP GSLRWFIAV 
IALVAVCCV LVGTALGAM RPAGRDHLT VSLLMIGVG 
IVLVTVSGV AWRLTSHDS STCRSMLGL GSTESVDVC 
TRRFVPRLP PSYGRPHHP YAAMMYPEF QYRPPPPSY 
QASMQEYRL RLLLLDRGN TPQIQAGIQ NAVSPPPTY 
RSHAGSLLR APLSNRRDT AQSEYSCPP SYRSQNSTN 
RPGTLQNSV LHSREQSLS LSESNQEES VNVVNILGS 
TEEDMALDN ITLDSLKME PESDINPLR MLLKGSSSD 
LEGSKDGNL VTIVQTSDQ SPVIVTVSG SSQIEGSTM 
QIPSEMEIL AHL
    CDS sequence
    • >TC034173 RA
      atggccact ttcaataat ttcccggaa gagggtcgt 
ccgccggac tcgccgtcc cggaagttc acccggaaa 
cattacccg ccgcggaat aactcctgg agtcacccc 
aatagctta atctgtgcg gaaaatgcc gaaatcaat 
cgtaacgtg ggcgataaa aagagccac agtctcaaa 
aacaagcta ttttgtatc aaaaagccc ctcgatgag 
gataaaacc acgttgaat atcctcaat gtccagaca 
ttgggcgat tcaagtgcc tctaaagcc gtttgtgat 
aatgacccg tcctgttcc cgtgatcgg aacaggaat 
tcgttccac caggacgtg gaggaggct ctgagttcg 
ctgttgtgg caaccgtac gagtaccag aactgcagc 
tcttgcagc agtagtgta acgttaagc gaccttgat 
gagtcgtcg ccaaacaaa ggcccttta cactcatca 
gacctaaat cttcgaacc caaatggtg aacaacctg 
aagccgtca cccagccaa tcgcggccc ggcaagccg 
tcacgtgac gcgttctct tgttatgct gcaagtagg 
ttgaacagt caagccagt ttactggag ttactcgac 
ggtggtacc ggtgatagt gtcagtgat tgtgatgtg 
ttgccctgc aaaaacagt gatatgcac ctggaagtc 
acggaaccc gaccccggt gccaccacc aacgtgctg 
agcatcccg acgggaacc catggcctg agcagcgcc 
aatgtcgtg ggtctgccc gagaacccg cgacgggcg 
tcggtacca tcgcggagc gtccatccg cgcaacggc 
tcggaaccc gtccgcacc caggacccc ataacccac 
ctccggagc gcctccgtg cccaagcaa atgcccaac 
gttagcaac atcaacgtg aatttctac cgagcggtt 
ccagtcaac gtcgtatct tcggtacca accatccaa 
tgcttgaat accctcgaa gggaacaat gtttccaac 
gtgcagatc atgccgctg ggctcggga accactcaa 
gtgacggtc cacaacccc acttccgtg aacgtggtg 
cgaaccttc acctccacg gaggcgcag acggacgag 
atcgccgtt tgtccgccg ccgccggag acgacggcc 
accaccagg gagcagaga cggaaggag cggcgggag 
cggcgccac caaagacga accaacggt accaatcat 
cgacatacg gtcgacagt ggtacccaa gccaatact 
ttgcaaaat gaaaggctt ccggatttg ctcaatagt 
cacatgccg ccgccgtat tcaccccta cctagtaat 
gtgccgccc cctaatgtg gtactaccg actcctatg 
atggtacca ccaccgcat cttcaaagt gttggacct 
aacaatgta gtgcctagt ggtttagtg gcgtttccg 
cccccgcaa gttgtggcg gggcaagga ccggtggcg 
gttccagtg ccgccgcct tcagggttt aggtttccg 
ttcccagcc gggggattc agaagaggc cgcttctcc 
gaggagccc cccaaaggc tgttgcggg ctgttggcg 
tggaaaccg ggctctttg cgctggttc atagcagtg 
atcgctttg gtggctgtc tgttgtgtg ttggttgga 
acagccctg ggtgccatg agacccgca ggacgggac 
catctcaca gtgtccctc ctcatgata ggtgtgggc 
atcgtcttg gtgacggtc agtggcgtg gcctggaga 
ctcacttcg cacgattcg tcaacttgt cgctcgatg 
ctcggctta ggttcgacc gaatcggtc gatgtgtgc 
actcgacgt ttcgttcct cgtttaccg ccgtcgtac 
ggccgtccc catcacccc tacgccgct atgatgtac 
ccagagttc cagtaccgg ccgccgccc cctagctac 
caagcctcc atgcaagaa tatagactg agacttttg 
cttctggac agaggcaac actccgcaa atccaggcc 
gggatacag aacgccgtc tcgccgcct ccgacgtac 
cgcagccac gctggcagt ctgcttagg gcgcctttg 
tcgaatcgg agagacacg gcacagtcg gagtacagt 
tgtcctccg tcttatcgt tcgcaaaac agtacgaat 
cgaccgggg actctacaa aattcggtt ctgcacagc 
cgggaacag agcttgagt ttgtcggag tcgaatcag 
gaagagtcg gtcaacgtt gtgaatatt ttaggcagt 
actgaagaa gacatggct ttagataat ataacttta 
gattcgttg aaaatggag ccggaaagt gatattaat 
ccgttgagg atgcttctt aaaggcagt tccagtgat 
cttgaaggt agcaaagac gggaatttg gtcactatt 
gtgcaaacg agcgaccaa agcccggtc attgttact 
gtgagcgga agctcgcag atcgaaggc agtacaatg 
caaatacca tccgaaatg gagatactg gcgcactta 
tag
    mRNA sequence
    • >TC034173 RA
      gatatttgc cttatgcaa atggcgaat cgttccgtt 
gaactatga ccgttccga tgaaaaact atggccact 
ttcaataat ttcccggaa gagggtcgt ccgccggac 
tcgccgtcc cggaagttc acccggaaa cattacccg 
ccgcggaat aactcctgg agtcacccc aatagctta 
atctgtgcg gaaaatgcc gaaatcaat cgtaacgtg 
ggcgataaa aagagccac agtctcaaa aacaagcta 
ttttgtatc aaaaagccc ctcgatgag gataaaacc 
acgttgaat atcctcaat gtccagaca ttgggcgat 
tcaagtgcc tctaaagcc gtttgtgat aatgacccg 
tcctgttcc cgtgatcgg aacaggaat tcgttccac 
caggacgtg gaggaggct ctgagttcg ctgttgtgg 
caaccgtac gagtaccag aactgcagc tcttgcagc 
agtagtgta acgttaagc gaccttgat gagtcgtcg 
ccaaacaaa ggcccttta cactcatca gacctaaat 
cttcgaacc caaatggtg aacaacctg aagccgtca 
cccagccaa tcgcggccc ggcaagccg tcacgtgac 
gcgttctct tgttatgct gcaagtagg ttgaacagt 
caagccagt ttactggag ttactcgac ggtggtacc 
ggtgatagt gtcagtgat tgtgatgtg ttgccctgc 
aaaaacagt gatatgcac ctggaagtc acggaaccc 
gaccccggt gccaccacc aacgtgctg agcatcccg 
acgggaacc catggcctg agcagcgcc aatgtcgtg 
ggtctgccc gagaacccg cgacgggcg tcggtacca 
tcgcggagc gtccatccg cgcaacggc tcggaaccc 
gtccgcacc caggacccc ataacccac ctccggagc 
gcctccgtg cccaagcaa atgcccaac gttagcaac 
atcaacgtg aatttctac cgagcggtt ccagtcaac 
gtcgtatct tcggtacca accatccaa tgcttgaat 
accctcgaa gggaacaat gtttccaac gtgcagatc 
atgccgctg ggctcggga accactcaa gtgacggtc 
cacaacccc acttccgtg aacgtggtg cgaaccttc 
acctccacg gaggcgcag acggacgag atcgccgtt 
tgtccgccg ccgccggag acgacggcc accaccagg 
gagcagaga cggaaggag cggcgggag cggcgccac 
caaagacga accaacggt accaatcat cgacatacg 
gtcgacagt ggtacccaa gccaatact ttgcaaaat 
gaaaggctt ccggatttg ctcaatagt cacatgccg 
ccgccgtat tcaccccta cctagtaat gtgccgccc 
cctaatgtg gtactaccg actcctatg atggtacca 
ccaccgcat cttcaaagt gttggacct aacaatgta 
gtgcctagt ggtttagtg gcgtttccg cccccgcaa 
gttgtggcg gggcaagga ccggtggcg gttccagtg 
ccgccgcct tcagggttt aggtttccg ttcccagcc 
gggggattc agaagaggc cgcttctcc gaggagccc 
cccaaaggc tgttgcggg ctgttggcg tggaaaccg 
ggctctttg cgctggttc atagcagtg atcgctttg 
gtggctgtc tgttgtgtg ttggttgga acagccctg 
ggtgccatg agacccgca ggacgggac catctcaca 
gtgtccctc ctcatgata ggtgtgggc atcgtcttg 
gtgacggtc agtggcgtg gcctggaga ctcacttcg 
cacgattcg tcaacttgt cgctcgatg ctcggctta 
ggttcgacc gaatcggtc gatgtgtgc actcgacgt 
ttcgttcct cgtttaccg ccgtcgtac ggccgtccc 
catcacccc tacgccgct atgatgtac ccagagttc 
cagtaccgg ccgccgccc cctagctac caagcctcc 
atgcaagaa tatagactg agacttttg cttctggac 
agaggcaac actccgcaa atccaggcc gggatacag 
aacgccgtc tcgccgcct ccgacgtac cgcagccac 
gctggcagt ctgcttagg gcgcctttg tcgaatcgg 
agagacacg gcacagtcg gagtacagt tgtcctccg 
tcttatcgt tcgcaaaac agtacgaat cgaccgggg 
actctacaa aattcggtt ctgcacagc cgggaacag 
agcttgagt ttgtcggag tcgaatcag gaagagtcg 
gtcaacgtt gtgaatatt ttaggcagt actgaagaa 
gacatggct ttagataat ataacttta gattcgttg 
aaaatggag ccggaaagt gatattaat ccgttgagg 
atgcttctt aaaggcagt tccagtgat cttgaaggt 
agcaaagac gggaatttg gtcactatt gtgcaaacg 
agcgaccaa agcccggtc attgttact gtgagcgga 
agctcgcag atcgaaggc agtacaatg caaatacca 
tccgaaatg gagatactg gcgcactta tagtcgaaa 
ctcccattc cttaaggtt agtgtgcag taatgtatc 
acgttgtat tataattat gcgatgaca tgtgcctat 
attgtgtag atagtttat aaatagcac tctgtatat 
tactattat attttaaac tgtataaaa gtaaatttt 
taataagaa ttgttaaac cgtctcttc attcaaaac 
caccctcct tttatcctt aaatggacc gacattttt 
ttaataaat tctccccca tatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY