Status
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Gene information
General information
TC034173
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005419,TC005418
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13455725..13483321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034173
    iBeetle screen iB_03947

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 2

    Fertility
    egg production: number <50% of expected -
    Comment: No phenotype found!!!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034173 RA
      MATFNNFPE EGRPPDSPS RKFTRKHYP PRNNSWSHP 
      NSLICAENA EINRNVGDK KSHSLKNKL FCIKKPLDE 
      DKTTLNILN VQTLGDSSA SKAVCDNDP SCSRDRNRN 
      SFHQDVEEA LSSLLWQPY EYQNCSSCS SSVTLSDLD 
      ESSPNKGPL HSSDLNLRT QMVNNLKPS PSQSRPGKP 
      SRDAFSCYA ASRLNSQAS LLELLDGGT GDSVSDCDV 
      LPCKNSDMH LEVTEPDPG ATTNVLSIP TGTHGLSSA 
      NVVGLPENP RRASVPSRS VHPRNGSEP VRTQDPITH 
      LRSASVPKQ MPNVSNINV NFYRAVPVN VVSSVPTIQ 
      CLNTLEGNN VSNVQIMPL GSGTTQVTV HNPTSVNVV 
      RTFTSTEAQ TDEIAVCPP PPETTATTR EQRRKERRE 
      RRHQRRTNG TNHRHTVDS GTQANTLQN ERLPDLLNS 
      HMPPPYSPL PSNVPPPNV VLPTPMMVP PPHLQSVGP 
      NNVVPSGLV AFPPPQVVA GQGPVAVPV PPPSGFRFP 
      FPAGGFRRG RFSEEPPKG CCGLLAWKP GSLRWFIAV 
      IALVAVCCV LVGTALGAM RPAGRDHLT VSLLMIGVG 
      IVLVTVSGV AWRLTSHDS STCRSMLGL GSTESVDVC 
      TRRFVPRLP PSYGRPHHP YAAMMYPEF QYRPPPPSY 
      QASMQEYRL RLLLLDRGN TPQIQAGIQ NAVSPPPTY 
      RSHAGSLLR APLSNRRDT AQSEYSCPP SYRSQNSTN 
      RPGTLQNSV LHSREQSLS LSESNQEES VNVVNILGS 
      TEEDMALDN ITLDSLKME PESDINPLR MLLKGSSSD 
      LEGSKDGNL VTIVQTSDQ SPVIVTVSG SSQIEGSTM 
      QIPSEMEIL AHL
    CDS sequence
    • >TC034173 RA
      atggccact ttcaataat ttcccggaa gagggtcgt 
      ccgccggac tcgccgtcc cggaagttc acccggaaa 
      cattacccg ccgcggaat aactcctgg agtcacccc 
      aatagctta atctgtgcg gaaaatgcc gaaatcaat 
      cgtaacgtg ggcgataaa aagagccac agtctcaaa 
      aacaagcta ttttgtatc aaaaagccc ctcgatgag 
      gataaaacc acgttgaat atcctcaat gtccagaca 
      ttgggcgat tcaagtgcc tctaaagcc gtttgtgat 
      aatgacccg tcctgttcc cgtgatcgg aacaggaat 
      tcgttccac caggacgtg gaggaggct ctgagttcg 
      ctgttgtgg caaccgtac gagtaccag aactgcagc 
      tcttgcagc agtagtgta acgttaagc gaccttgat 
      gagtcgtcg ccaaacaaa ggcccttta cactcatca 
      gacctaaat cttcgaacc caaatggtg aacaacctg 
      aagccgtca cccagccaa tcgcggccc ggcaagccg 
      tcacgtgac gcgttctct tgttatgct gcaagtagg 
      ttgaacagt caagccagt ttactggag ttactcgac 
      ggtggtacc ggtgatagt gtcagtgat tgtgatgtg 
      ttgccctgc aaaaacagt gatatgcac ctggaagtc 
      acggaaccc gaccccggt gccaccacc aacgtgctg 
      agcatcccg acgggaacc catggcctg agcagcgcc 
      aatgtcgtg ggtctgccc gagaacccg cgacgggcg 
      tcggtacca tcgcggagc gtccatccg cgcaacggc 
      tcggaaccc gtccgcacc caggacccc ataacccac 
      ctccggagc gcctccgtg cccaagcaa atgcccaac 
      gttagcaac atcaacgtg aatttctac cgagcggtt 
      ccagtcaac gtcgtatct tcggtacca accatccaa 
      tgcttgaat accctcgaa gggaacaat gtttccaac 
      gtgcagatc atgccgctg ggctcggga accactcaa 
      gtgacggtc cacaacccc acttccgtg aacgtggtg 
      cgaaccttc acctccacg gaggcgcag acggacgag 
      atcgccgtt tgtccgccg ccgccggag acgacggcc 
      accaccagg gagcagaga cggaaggag cggcgggag 
      cggcgccac caaagacga accaacggt accaatcat 
      cgacatacg gtcgacagt ggtacccaa gccaatact 
      ttgcaaaat gaaaggctt ccggatttg ctcaatagt 
      cacatgccg ccgccgtat tcaccccta cctagtaat 
      gtgccgccc cctaatgtg gtactaccg actcctatg 
      atggtacca ccaccgcat cttcaaagt gttggacct 
      aacaatgta gtgcctagt ggtttagtg gcgtttccg 
      cccccgcaa gttgtggcg gggcaagga ccggtggcg 
      gttccagtg ccgccgcct tcagggttt aggtttccg 
      ttcccagcc gggggattc agaagaggc cgcttctcc 
      gaggagccc cccaaaggc tgttgcggg ctgttggcg 
      tggaaaccg ggctctttg cgctggttc atagcagtg 
      atcgctttg gtggctgtc tgttgtgtg ttggttgga 
      acagccctg ggtgccatg agacccgca ggacgggac 
      catctcaca gtgtccctc ctcatgata ggtgtgggc 
      atcgtcttg gtgacggtc agtggcgtg gcctggaga 
      ctcacttcg cacgattcg tcaacttgt cgctcgatg 
      ctcggctta ggttcgacc gaatcggtc gatgtgtgc 
      actcgacgt ttcgttcct cgtttaccg ccgtcgtac 
      ggccgtccc catcacccc tacgccgct atgatgtac 
      ccagagttc cagtaccgg ccgccgccc cctagctac 
      caagcctcc atgcaagaa tatagactg agacttttg 
      cttctggac agaggcaac actccgcaa atccaggcc 
      gggatacag aacgccgtc tcgccgcct ccgacgtac 
      cgcagccac gctggcagt ctgcttagg gcgcctttg 
      tcgaatcgg agagacacg gcacagtcg gagtacagt 
      tgtcctccg tcttatcgt tcgcaaaac agtacgaat 
      cgaccgggg actctacaa aattcggtt ctgcacagc 
      cgggaacag agcttgagt ttgtcggag tcgaatcag 
      gaagagtcg gtcaacgtt gtgaatatt ttaggcagt 
      actgaagaa gacatggct ttagataat ataacttta 
      gattcgttg aaaatggag ccggaaagt gatattaat 
      ccgttgagg atgcttctt aaaggcagt tccagtgat 
      cttgaaggt agcaaagac gggaatttg gtcactatt 
      gtgcaaacg agcgaccaa agcccggtc attgttact 
      gtgagcgga agctcgcag atcgaaggc agtacaatg 
      caaatacca tccgaaatg gagatactg gcgcactta 
      tag
    mRNA sequence
    • >TC034173 RA
      gatatttgc cttatgcaa atggcgaat cgttccgtt 
      gaactatga ccgttccga tgaaaaact atggccact 
      ttcaataat ttcccggaa gagggtcgt ccgccggac 
      tcgccgtcc cggaagttc acccggaaa cattacccg 
      ccgcggaat aactcctgg agtcacccc aatagctta 
      atctgtgcg gaaaatgcc gaaatcaat cgtaacgtg 
      ggcgataaa aagagccac agtctcaaa aacaagcta 
      ttttgtatc aaaaagccc ctcgatgag gataaaacc 
      acgttgaat atcctcaat gtccagaca ttgggcgat 
      tcaagtgcc tctaaagcc gtttgtgat aatgacccg 
      tcctgttcc cgtgatcgg aacaggaat tcgttccac 
      caggacgtg gaggaggct ctgagttcg ctgttgtgg 
      caaccgtac gagtaccag aactgcagc tcttgcagc 
      agtagtgta acgttaagc gaccttgat gagtcgtcg 
      ccaaacaaa ggcccttta cactcatca gacctaaat 
      cttcgaacc caaatggtg aacaacctg aagccgtca 
      cccagccaa tcgcggccc ggcaagccg tcacgtgac 
      gcgttctct tgttatgct gcaagtagg ttgaacagt 
      caagccagt ttactggag ttactcgac ggtggtacc 
      ggtgatagt gtcagtgat tgtgatgtg ttgccctgc 
      aaaaacagt gatatgcac ctggaagtc acggaaccc 
      gaccccggt gccaccacc aacgtgctg agcatcccg 
      acgggaacc catggcctg agcagcgcc aatgtcgtg 
      ggtctgccc gagaacccg cgacgggcg tcggtacca 
      tcgcggagc gtccatccg cgcaacggc tcggaaccc 
      gtccgcacc caggacccc ataacccac ctccggagc 
      gcctccgtg cccaagcaa atgcccaac gttagcaac 
      atcaacgtg aatttctac cgagcggtt ccagtcaac 
      gtcgtatct tcggtacca accatccaa tgcttgaat 
      accctcgaa gggaacaat gtttccaac gtgcagatc 
      atgccgctg ggctcggga accactcaa gtgacggtc 
      cacaacccc acttccgtg aacgtggtg cgaaccttc 
      acctccacg gaggcgcag acggacgag atcgccgtt 
      tgtccgccg ccgccggag acgacggcc accaccagg 
      gagcagaga cggaaggag cggcgggag cggcgccac 
      caaagacga accaacggt accaatcat cgacatacg 
      gtcgacagt ggtacccaa gccaatact ttgcaaaat 
      gaaaggctt ccggatttg ctcaatagt cacatgccg 
      ccgccgtat tcaccccta cctagtaat gtgccgccc 
      cctaatgtg gtactaccg actcctatg atggtacca 
      ccaccgcat cttcaaagt gttggacct aacaatgta 
      gtgcctagt ggtttagtg gcgtttccg cccccgcaa 
      gttgtggcg gggcaagga ccggtggcg gttccagtg 
      ccgccgcct tcagggttt aggtttccg ttcccagcc 
      gggggattc agaagaggc cgcttctcc gaggagccc 
      cccaaaggc tgttgcggg ctgttggcg tggaaaccg 
      ggctctttg cgctggttc atagcagtg atcgctttg 
      gtggctgtc tgttgtgtg ttggttgga acagccctg 
      ggtgccatg agacccgca ggacgggac catctcaca 
      gtgtccctc ctcatgata ggtgtgggc atcgtcttg 
      gtgacggtc agtggcgtg gcctggaga ctcacttcg 
      cacgattcg tcaacttgt cgctcgatg ctcggctta 
      ggttcgacc gaatcggtc gatgtgtgc actcgacgt 
      ttcgttcct cgtttaccg ccgtcgtac ggccgtccc 
      catcacccc tacgccgct atgatgtac ccagagttc 
      cagtaccgg ccgccgccc cctagctac caagcctcc 
      atgcaagaa tatagactg agacttttg cttctggac 
      agaggcaac actccgcaa atccaggcc gggatacag 
      aacgccgtc tcgccgcct ccgacgtac cgcagccac 
      gctggcagt ctgcttagg gcgcctttg tcgaatcgg 
      agagacacg gcacagtcg gagtacagt tgtcctccg 
      tcttatcgt tcgcaaaac agtacgaat cgaccgggg 
      actctacaa aattcggtt ctgcacagc cgggaacag 
      agcttgagt ttgtcggag tcgaatcag gaagagtcg 
      gtcaacgtt gtgaatatt ttaggcagt actgaagaa 
      gacatggct ttagataat ataacttta gattcgttg 
      aaaatggag ccggaaagt gatattaat ccgttgagg 
      atgcttctt aaaggcagt tccagtgat cttgaaggt 
      agcaaagac gggaatttg gtcactatt gtgcaaacg 
      agcgaccaa agcccggtc attgttact gtgagcgga 
      agctcgcag atcgaaggc agtacaatg caaatacca 
      tccgaaatg gagatactg gcgcactta tagtcgaaa 
      ctcccattc cttaaggtt agtgtgcag taatgtatc 
      acgttgtat tataattat gcgatgaca tgtgcctat 
      attgtgtag atagtttat aaatagcac tctgtatat 
      tactattat attttaaac tgtataaaa gtaaatttt 
      taataagaa ttgttaaac cgtctcttc attcaaaac 
      caccctcct tttatcctt aaatggacc gacattttt 
      ttaataaat tctccccca tatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY