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Gene information
General information
TC005415 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13504553..13508791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005415
    iBeetle screen iB_03946

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen posterior circumference decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    elytra dorsal orientation not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005415 RA
      MAESKAVDD LLLGDGFSI VDSTKGDAP ESESKKRSS 
      KDKDKEKKP EVKKEKSEE KKPKEEPPK DDTKVNGHD 
      TKSDALLIT TEEDLDMTE KDKKSEAET KDEDSKPKK 
      DSESGSKEK DKPVDKKKQ GDLWVSNIT RHVKATDLK 
      KHFSRHGKV LTAKIVTNG KSFFGYILM ETSDIANKC 
      VRSLHGSTL DGRKLTVSR QRPDQRRPM TERKPTVKS 
      SKTERKTEK KSDEKKTDK KDDALAAAK RTEDRLRRE 
      IDFHKREIT RLKRYLTDE QRRLRIEQQ KNRSLGREL 
      QEAEAKLRD ERRKMDRER ELFEKNQRT DRVKLENDR 
      SMIKKELEE VKKLREYLK RKLEEEARP VDRRPRASS 
      PYAYSSKRF RESSGGDSK SKYVEERTR TPPSPPKLS 
      SRSKRSPVN SFVEKDRRP HHYSMSERE EVRSLVIPA 
      KPSAPAAPR NIWPSFHQE VWRVPPGTA TSSPFSGQS 
      YSRQSEYSS STSKGPSYS SSQREFSKR DHYTSSSVR 
      KY
    CDS sequence
    • >TC005415 RA
      atggccgag tccaaggct gttgacgat ttgctcctc 
      ggtgatggc ttctccatt gtcgatagt acgaaaggg 
      gacgctccc gaatccgaa tccaaaaag cgatcatcg 
      aaggacaaa gacaaggag aagaagccc gaagtgaag 
      aaagaaaaa tccgaggag aagaaacca aaggaagag 
      cccccgaag gatgacacc aaagtcaac ggacacgac 
      accaaaagt gacgccctc ctcatcaca acggaggaa 
      gatttggac atgaccgag aaggacaag aagagcgaa 
      gccgaaacc aaagacgaa gactccaag ccgaagaaa 
      gactccgaa agcggcagc aaagagaag gacaaacca 
      gtcgataag aagaaacaa ggcgacctc tgggtctcc 
      aacatcacc cggcatgtc aaggccacc gacttgaag 
      aaacacttc tcccgccat ggcaaggtc ctaaccgcc 
      aaaatcgtc accaacggc aagtccttc ttcggctac 
      attctcatg gagacgtcc gacatcgcc aacaagtgt 
      gtgcggagt ctccacggc tcgaccctc gacggccgc 
      aaactcacc gtgagccgt cagagaccc gaccagagg 
      agacccatg accgagcgc aagcccaca gtcaagtcc 
      agcaagacc gagcgcaaa accgaaaaa aagtccgac 
      gagaagaaa accgacaag aaagacgac gccttggct 
      gcggcgaaa cgcacggaa gaccgactt cgccgcgaa 
      atcgacttc cacaagcgc gaaatcaca cgactaaag 
      cgctacctc accgacgaa caacgacgc ttgcgaatc 
      gaacaacag aagaatcgt tcgctgggt cgggaactg 
      caagaagcc gaagccaaa ctccgcgac gaacgcaga 
      aaaatggac cgcgaacgc gaactgttc gagaagaac 
      caacgcacc gaccgggtc aagctcgaa aacgaccgc 
      tccatgatc aagaaggag ctggaagaa gtgaagaaa 
      ctccgcgag tatctcaag cgcaagctg gaggaagag 
      gcgcgtccg gtggaccgg cggccgcgg gcttcgtca 
      ccgtacgcc tactcgtcg aagaggttc cgggagtcg 
      tcgggcggc gactccaag tccaagtac gtggaggag 
      cggacgagg acgccgccg tcgcccccg aagctctcc 
      tccaggtcc aaacgctcg ccggtcaat tccttcgtg 
      gagaaggat cgaaggccg catcattat agcatgtcc 
      gagagggag gaagtccgc tcgttggtg attccggcc 
      aagccgtcg gcaccggcc gcccccagg aatatatgg 
      ccgtcgttc caccaggaa gtttggagg gtgcctccg 
      gggacggcg accagttcg ccgttttca ggccagtcg 
      tatagcaga cagtccgag tatagtagc tccacgtcg 
      aaggggcca agttattcg tcgtcgcag agggagttc 
      agcaaacgc gatcattat acgagcagt tcagttagg 
      aagtattaa
    mRNA sequence
    • >TC005415 RA
      catcgattt agcgattga ttttctttg tttgtttgc 
      ttgttgtcg gaaaacagg catcgatcg ttgcatttt 
      cacaatgct gtccgaaag gccgtaaac ttccaactc 
      tccctaaaa acgtacgac tgcttttag cggccccga 
      cggctttgc gggtgtaat tcactctct cgcccccgc 
      ctttagcag aaggaaaac attctccac ggtcgggga 
      ttatcgccg aattcactt tccatattc ggaaggaaa 
      ctcggggaa attaatcga atcgcgtga tggaaataa 
      caaaaacat gatgggttg ttttaacga gatttccgc 
      gctcggaca ccgctcgta attgtaact ttttgacgg 
      aatgtgaga ttttaaacg acttgacaa agttccgta 
      tatatgaaa tgtttattt cttttaacc gtcctgagg 
      tacagcgta tttttattt catcaagac ccagcattg 
      gaatttaaa gctacgtcg aaaaaggct acgagggag 
      ggagttgcc cgttttctt tgcaaatat gagtagttt 
      agttgcaag gtgtcgcta ggtatagtc ttgtccccg 
      tttcgacat taatggcgc ataaatatc cagctcggc 
      tctttgttt tccgtcggg ggagcccct ctgtcagga 
      gcccgcata attaggagc gctgagcgt ctgcggctg 
      cacccgaca cgccatcgt ggacccggt gacttatgg 
      cctacattt ccgcaactc ctgggggcg gccaccgcg 
      ccgatcgaa gaggagaaa tagaggcaa gaggaggcc 
      gccgccact ggacacgtt ttggggcat ttttcactt 
      ggcgccata aatatgcac gctcgacgc gtctctaac 
      gttgaataa taattaggg cggcggcgg cggcggcgg 
      tggcgggct cccatgttt catttatac gccgtcgcc 
      gcagatgtg tacaacttc cgatgccaa tcaactaaa 
      ttactaaac aacttggac gatgaattt aatatgact 
      ttgctttga aactcgcta tacccacct atcgttagt 
      tttttgccg cgtctttca acttgttat cggagtaca 
      aattgctac tggtggacg agatagcag gcgacgaaa 
      ggaaaataa ccaccaaag tatacaagt cttatctgt 
      ggactacaa tgattccgc tccggctta tccttgcaa 
      ggaacctat ctgaatgtg tgtgctgtg tgttacaac 
      aaaacgact cgaagctcc ccattttat tactgtgtc 
      tgttggctg ttgtagcac aatagttga attttttgg 
      tactagctt tgcccccgc taccaaaaa atttatttt 
      gctacactg gccttgctt gtgttctaa caaaagtct 
      gattttacg caggtaaat aataggatt tcctgcaat 
      agtatttaa aaaataacc aatggccga gtccaaggc 
      tgttgacga tttgctcct cggtgatgg cttctccat 
      tgtcgatag tacgaaagg ggacgctcc cgaatccga 
      atccaaaaa gcgatcatc gaaggacaa agacaagga 
      gaagaagcc cgaagtgaa gaaagaaaa atccgagga 
      gaagaaacc aaaggaaga gcccccgaa ggatgacac 
      caaagtcaa cggacacga caccaaaag tgacgccct 
      cctcatcac aacggagga agatttgga catgaccga 
      gaaggacaa gaagagcga agccgaaac caaagacga 
      agactccaa gccgaagaa agactccga aagcggcag 
      caaagagaa ggacaaacc agtcgataa gaagaaaca 
      aggcgacct ctgggtctc caacatcac ccggcatgt 
      caaggccac cgacttgaa gaaacactt ctcccgcca 
      tggcaaggt cctaaccgc caaaatcgt caccaacgg 
      caagtcctt cttcggcta cattctcat ggagacgtc 
      cgacatcgc caacaagtg tgtgcggag tctccacgg 
      ctcgaccct cgacggccg caaactcac cgtgagccg 
      tcagagacc cgaccagag gagacccat gaccgagcg 
      caagcccac agtcaagtc cagcaagac cgagcgcaa 
      aaccgaaaa aaagtccga cgagaagaa aaccgacaa 
      gaaagacga cgccttggc tgcggcgaa acgcacgga 
      agaccgact tcgccgcga aatcgactt ccacaagcg 
      cgaaatcac acgactaaa gcgctacct caccgacga 
      acaacgacg cttgcgaat cgaacaaca gaagaatcg 
      ttcgctggg tcgggaact gcaagaagc cgaagccaa 
      actccgcga cgaacgcag aaaaatgga ccgcgaacg 
      cgaactgtt cgagaagaa ccaacgcac cgaccgggt 
      caagctcga aaacgaccg ctccatgat caagaagga 
      gctggaaga agtgaagaa actccgcga gtatctcaa 
      gcgcaagct ggaggaaga ggcgcgtcc ggtggaccg 
      gcggccgcg ggcttcgtc accgtacgc ctactcgtc 
      gaagaggtt ccgggagtc gtcgggcgg cgactccaa 
      gtccaagta cgtggagga gcggacgag gacgccgcc 
      gtcgccccc gaagctctc ctccaggtc caaacgctc 
      gccggtcaa ttccttcgt ggagaagga tcgaaggcc 
      gcatcatta tagcatgtc cgagaggga ggaagtccg 
      ctcgttggt gattccggc caagccgtc ggcaccggc 
      cgcccccag gaatatatg gccgtcgtt ccaccagga 
      agtttggag ggtgcctcc ggggacggc gaccagttc 
      gccgttttc aggccagtc gtatagcag acagtccga 
      gtatagtag ctccacgtc gaaggggcc aagttattc 
      gtcgtcgca gagggagtt cagcaaacg cgatcatta 
      tacgagcag ttcagttag gaagtatta attgtattg 
      gtttagttg tagtgttgc gttttttgg aaataaagc 
      cgtagatta gaagagaaa tttttaata aagtatcgt 
      cgtaacctt atttgaagt cgtttcgtg taatttgcc 
      aagtgcgaa atttaattt tgcgaagcg gaactttca 
      tctcatctc tggataaaa tgagcaata tattccgac 
      attacttat tttattaga ttaaaatct accagaaca 
      aactaggat acgaattac caaactaat ggcggaatt 
      attgtgcag acctttcac ttatctcga tttacattt 
      ttaacaggg atttgcagg gcgagggta agtcgcgat 
      tatatgcat cctcctgct cgaataaat ttttttctt 
      ctct
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