Status
Please wait ...
Gene information
General information
TC005381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13920618..13921653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005381
    iBeetle screen iB_03945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005381 RA
      MGQSQSTRR LKSIKTNVC EIAEKIRND ELSAREIDR 
      SHEILLDYV KELKYILGK NLRASKIRK VEKAIIGAL 
      QDLATLEGS LKTIEPRLK AKSMQTLAD IEKNIKTIG 
      DNLLNQNFE LDKEVISKY KKRISFIDG VDDDIGVRK 
      NELLDVVTC VESALSERE IEQQLKQAE EKHYNLNDN 
      KTLTELRNK VGEIPEVSE KVTKRKNYL LYNIDNTLV 
      ALSVTNPNL VTDVVERYN RFENSFTLN CNLDELVSV 
      FEQLSQLKV KLEQRLKVL EQQIELEKI DPFAEKIPV 
      KETNDKLNQ IREAVGKIQ T
    CDS sequence
    • >TC005381 RA
      atgggacaa agccagtca acccgacgc ctcaaatcg 
      atcaaaacc aacgtgtgt gaaatcgcc gaaaaaatt 
      cgaaacgac gaactttcg gctcgagaa atcgaccga 
      tcgcatgaa attctcctc gactacgtc aaagaactc 
      aagtacatc ttaggcaaa aacctacgg gcttccaaa 
      atccgaaaa gtcgagaag gccataatt ggggccttg 
      caagacctc gccaccttg gaaggctct ctgaaaacg 
      atcgaaccc cgacttaag gcaaaatcg atgcaaacc 
      ctcgccgac attgaaaaa aacatcaaa acgatcggt 
      gacaattta cttaaccag aacttcgag ctggacaag 
      gaagtgatc agcaagtac aagaaacgg atcagtttt 
      atcgacggt gttgacgac gatattggc gttcgcaag 
      aacgaactc ctggacgtt gtgacatgt gttgagagt 
      gctctgagt gaacgggaa attgaacaa cagttgaag 
      caagccgag gaaaaacac tacaacttg aacgacaac 
      aaaacacta actgaactg cgaaacaaa gttggggaa 
      attcctgaa gttagcgag aaagtgacg aaacgtaaa 
      aactatctc ttgtacaac attgacaac actttagtg 
      gccctaagt gttacgaac cctaacttg gtcactgac 
      gttgttgaa cgttacaac agattcgaa aattcgttc 
      acacttaac tgcaacttg gacgaactt gtgagtgtt 
      tttgagcaa ctgtcccaa ctgaaagtg aagctagag 
      caaagactt aaagtgtta gaacaacaa atcgaactc 
      gagaaaatt gatcctttt gccgaaaaa atcccagtg 
      aaggaaact aacgacaaa ctaaaccaa attagagaa 
      gccgttggc aaaattcaa acctaa
    mRNA sequence
    • >TC005381 RA
      aaggtgtgt cttgtgatt tacattaaa aatgggaca 
      aagccagtc aacccgacg cctcaaatc gatcaaaac 
      caacgtgtg tgaaatcgc cgaaaaaat tcgaaacga 
      cgaactttc ggctcgaga aatcgaccg atcgcatga 
      aattctcct cgactacgt caaagaact caagtacat 
      cttaggcaa aaacctacg ggcttccaa aatccgaaa 
      agtcgagaa ggccataat tggggcctt gcaagacct 
      cgccacctt ggaaggctc tctgaaaac gatcgaacc 
      ccgacttaa ggcaaaatc gatgcaaac cctcgccga 
      cattgaaaa aaacatcaa aacgatcgg tgacaattt 
      acttaacca gaacttcga gctggacaa ggaagtgat 
      cagcaagta caagaaacg gatcagttt tatcgacgg 
      tgttgacga cgatattgg cgttcgcaa gaacgaact 
      cctggacgt tgtgacatg tgttgagag tgctctgag 
      tgaacggga aattgaaca acagttgaa gcaagccga 
      ggaaaaaca ctacaactt gaacgacaa caaaacact 
      aactgaact gcgaaacaa agttgggga aattcctga 
      agttagcga gaaagtgac gaaacgtaa aaactatct 
      cttgtacaa cattgacaa cactttagt ggccctaag 
      tgttacgaa ccctaactt ggtcactga cgttgttga 
      acgttacaa cagattcga aaattcgtt cacacttaa 
      ctgcaactt ggacgaact tgtgagtgt ttttgagca 
      actgtccca actgaaagt gaagctaga gcaaagact 
      taaagtgtt agaacaaca aatcgaact cgagaaaat 
      tgatccttt tgccgaaaa aatcccagt gaaggaaac 
      taacgacaa actaaacca aattagaga agccgttgg 
      caaaattca aacctaaat ttcccgctt gtcctacag 
      tgaaaacag ccacattta caaaagttt ccacaaagc 
      gttgcaaaa atacaagac tgaagcgag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY