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Gene information
General information
TC005371 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 13994059..13995378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005371
    iBeetle screen iB_03944

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    ommatidia sometimes number decreased - Number of animals showing the phenotype: 4

    eyes shape irregular - Number of animals showing the phenotype: 2

    oblique dorsal thoracic muscles potentially structure irregular - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005371 RA
      MTRPSVYIT RKINEEALK LLSATCDIT SWPGPDPVP 
      RSELLKNIP GKDALFCML TDKIDKEVL EKADKLKVI 
      STMSVGYDH LEIPEIKKR QIKIGYTPD ILTDATAEL 
      TVALLLATS RRLLEANAE ARTGGWKAW APFWMCGPG 
      LGGATVGIV GFGRIGQEV AKRLKPFNT RQILYHSRS 
      AKKEAEEIG ARKVTLDEL LTQSDFVVV CCALTPETK 
      GMFNEGAFK KMKKSAVFV NTSRGAVVD QDALVRALQ 
      SGEIWGAGL DVMTPEPLP LDDPLFGLK NCVILPHIG 
      SACIETRNQ MAVLTANNI LEALRGGKM PAELIL
    CDS sequence
    • >TC005371 RA
      atgacccga ccttcggtc tacataaca cgcaaaatc 
      aacgaagaa gccctcaaa ttactatca gcgacctgt 
      gatataaca tcatggcca ggaccggat ccagtcccg 
      cgctccgaa ctcctaaaa aacatcccc ggaaaggac 
      gcccttttc tgcatgctc accgacaag atcgacaaa 
      gaggtgctt gaaaaagcc gacaaactc aaagtaatc 
      tcgaccatg tcggtgggt tacgaccac ctggaaatc 
      cccgaaata aaaaagcgc caaatcaaa atcgggtac 
      acccccgat attctcacc gacgctacg gccgaactc 
      accgtggct ttgctccta gccaccagt cggcgcctc 
      cttgaggcc aacgccgag gcgcggact gggggctgg 
      aaggcctgg gcccccttc tggatgtgc ggcccaggc 
      ctcgggggc gccactgtg ggcattgtg ggcttcggc 
      cgaattggc caagaggtg gccaagcgg ctaaagccc 
      ttcaacacg cgtcagatt ctttaccac agccgatcg 
      gctaaaaaa gaggccgag gaaatcggg gcccggaaa 
      gtcactcta gatgagttg ctcacacaa agcgatttt 
      gtggtcgtt tgttgtgcg ttgaccccc gaaacgaag 
      gggatgttc aacgagggg gcgtttaaa aaaatgaaa 
      aaaagcgcc gtttttgtt aatacgagt cgaggggct 
      gttgtggat caggatgcc ttggttagg gcgcttcag 
      agtggggag atttggggc gctgggttg gatgtgatg 
      actcctgag cccttgccc ctcgatgat ccgcttttt 
      gggctgaaa aattgtgtc atcttgccg cacattggc 
      agtgcgtgt attgagaca aggaatcag atggctgtt 
      cttacagct aataatatt ttagaggct ttaagaggt 
      ggcaaaatg cctgccgag ctgattctg taa
    mRNA sequence
    • >TC005371 RA
      aagccggcg acattgcaa aatgccaac cagccaata 
      attaaaaaa ttaacttca caattaaat gctttttac 
      cacaaaaat gactgtcca atttggctt ttctttgac 
      ccgactttc tgcaacaat tactttcac tccccaacg 
      atttcttcc aaagtccag tcggacatt gctagtgca 
      aaaaaccaa aattcacac aaattgccc aaaaatgac 
      ccgaccttc ggtctacat aacacgcaa aatcaacga 
      agaagccct caaattact atcagcgac ctgtgatat 
      aacatcatg gccaggacc ggatccagt cccgcgctc 
      cgaactcct aaaaaacat ccccggaaa ggacgccct 
      tttctgcat gctcaccga caagatcga caaagaggt 
      gcttgaaaa agccgacaa actcaaagt aatctcgac 
      catgtcggt gggttacga ccacctgga aatccccga 
      aataaaaaa gcgccaaat caaaatcgg gtacacccc 
      cgatattct caccgacgc tacggccga actcaccgt 
      ggctttgct cctagccac cagtcggcg cctccttga 
      ggccaacgc cgaggcgcg gactggggg ctggaaggc 
      ctgggcccc cttctggat gtgcggccc aggcctcgg 
      gggcgccac tgtgggcat tgtgggctt cggccgaat 
      tggccaaga ggtggccaa gcggctaaa gcccttcaa 
      cacgcgtca gattcttta ccacagccg atcggctaa 
      aaaagaggc cgaggaaat cggggcccg gaaagtcac 
      tctagatga gttgctcac acaaagcga ttttgtggt 
      cgtttgttg tgcgttgac ccccgaaac gaaggggat 
      gttcaacga gggggcgtt taaaaaaat gaaaaaaag 
      cgccgtttt tgttaatac gagtcgagg ggctgttgt 
      ggatcagga tgccttggt tagggcgct tcagagtgg 
      ggagatttg gggcgctgg gttggatgt gatgactcc 
      tgagccctt gcccctcga tgatccgct ttttgggct 
      gaaaaattg tgtcatctt gccgcacat tggcagtgc 
      gtgtattga gacaaggaa tcagatggc tgttcttac 
      agctaataa tattttaga ggctttaag aggtggcaa 
      aatgcctgc cgagctgat tctgtaaaa ggggaaaaa 
      ataaagaat ttctaatga tttcgccgt gtttttgtt 
      tctaatttt acaccaatt catgttaat aaaccgttc 
      taaatgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY