Status
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Gene information
General information
TC005359 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14096606..14101558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005359
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03943

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005359 RA
      MAESVTLVT LSQMIDLSL NCNEGIINF HVLHTFLHV 
      VVQQLKMHD VAVEFRGSD SEKIQALIS TVKPTSTHL 
      LPKYNVVPE ESVKLKKKT GPHQKKKAS LQDVMAEKE 
      RQKLEEKKE KEIRVIERR KDSKEDPDL NPLPLKSPP 
      GSLLGITPA DLQTVVVVE SQSTLPQYT VALTKTTFD 
      SLQNDLDSL KIQLKELQE LPANLGLIG ATRSQSSPI 
      LDMFQILHL TKRMDANET ALEKIGSLI EDLVKSDKG 
      QMPPTIIER VQEVRASVD ETQKYTAER RLSALNEVE 
      VRLTNLENA VNNLNENIA AVATGVGEF KPQEPILEK 
      PASAQPSLG PSDMASVAR TETSSVVPE GAKSPSRKS 
      QGTESVKIM APQVVYQSQ IQDVDLGEL PSNQALILI 
      QEEFQNLKN WMTESIQEV DKLKAFAPP APGEVDTRV 
      VDSKFNECS RKINELNDL YNTQIKLLS EQFNSVQSE 
      MNEVATKLD IILPGGDST GISAETTMD LANKLTLLE 
      KEMIAISDK AEELQQEKE QRDKQTEVI MEQIDILKI 
      VKANREDLE DALGEKADA CMINRKVSH DQFDAACDD 
      LYRGIEEAL SKLEEQEEM WHQALDSIQ REVGNKLDK 
      MEVDPLRDF VNSKLRALQ EKFKSLSAL KREQEAAGT 
      KSRFLRNVN CISCDKDVI MRKEMDASL KPKAYAMPA 
      SRNMAPYLA YELDQLRKQ QKCVPNSKN LNFIESALK 
      TRGNKEKNH LCNRYCGGS HTTTTPQQR VTRLGHFLE 
      QWGPEIAPL QDEYIRGND NHMYKARDE AIYTKKGSK 
      VENGPKGSI RPSTAGGAP MMEQEVPQG RNTYVSAEA 
      HMVVSKEAE EAANKKSLN IGAVPIEEV TQASQQIPV 
      KVSIEEPVS QKASQLGKA ASQLSNEQL RKTQSAEGK 
      SSQLSGERK SATIEGKSS QLSGERKSA TPEGKSSQL 
      SGERKSAAI EGKSSQLSN EQKRSQSAI VGKKGSEQA 
      VEKGQE
    CDS sequence
    • >TC005359 RA
      atggccgaa agcgttaca cttgtaaca ctctcacaa 
      atgatcgat ttatcgtta aattgcaac gagggcatt 
      atcaacttc cacgtcctc cacaccttc ctccacgtc 
      gttgtccaa caactaaaa atgcacgac gtagctgtg 
      gaattccga ggctccgac agcgaaaaa atccaagcc 
      ttgatcagt accgtcaaa cctacctcc acccacctc 
      ctcccaaaa tacaacgtc gtgcctgaa gaaagcgtc 
      aagttgaag aaaaaaaca ggaccccat cagaagaaa 
      aaagccagt ttacaggac gtgatggcg gaaaaagag 
      cgccagaag ttggaagag aaaaaagag aaggaaatc 
      cgtgttatc gaacgccgg aaagactca aaggaagac 
      cctgacctt aaccccctc ccgttgaaa tcaccccca 
      ggtagcctc cttggaatc actcctgcc gacctccag 
      acggttgtc gtggtcgag tcccagtca accctcccc 
      cagtacact gtggccctc accaaaacc accttcgac 
      agcctccaa aatgaccta gactcgctc aaaatccaa 
      ctcaaggag ttacaagaa ctcccagct aatctgggc 
      ttaattggg gccacgagg tcccagtca agcccgatc 
      ctggacatg ttccaaatc ctccacttg accaaacga 
      atggacgcc aatgagaca gcccttgag aaaatcggt 
      tcgttgatt gaagacttg gtcaagagc gacaaaggg 
      cagatgccc ccaaccatc atcgaaagg gtccaagaa 
      gtgcgcgct tcagtcgac gaaacccaa aaatacaca 
      gctgaacgg cggttaagt gcgttaaac gaggtcgaa 
      gtcaggcta actaacctt gaaaatgct gtcaataac 
      ttgaatgag aatatagca gctgtggcc acaggagtc 
      ggcgaattt aaaccgcaa gagccaatt ttggaaaaa 
      ccagcgtcg gctcagccc agtttgggg ccctctgat 
      atggccagc gttgccaga actgagact agtagtgtg 
      gtccccgaa ggggccaaa tctccctcg aggaaaagc 
      caagggacg gagtcggtc aaaatcatg gccccgcag 
      gtggtctac cagagccag atccaagat gtcgatttg 
      ggtgagttg ccctcgaac caggcccta atcctgatc 
      caggaggag ttccaaaat ttgaagaac tggatgact 
      gagagtatc caagaagtg gacaagttg aaagctttc 
      gctccgcca gcgcctggc gaagttgac acgcgagtt 
      gttgacagc aaattcaac gaatgtagt cggaaaatc 
      aacgaactg aacgactta tacaacacg cagatcaag 
      ctcctcagt gagcagttc aacagcgtc cagagcgaa 
      atgaacgaa gttgccacc aaacttgac ataattctc 
      ccgggaggt gattcgacc gggattagt gccgagacg 
      accatggat ttggccaat aaactcacg ctgcttgag 
      aaggagatg attgcaatt tcggataag gccgaagag 
      ttgcaacag gagaaggaa cagcgtgat aagcagact 
      gaagtgatt atggaacag attgatatt ttgaaaatt 
      gtgaaagcg aatcgggaa gatttggag gatgcgttg 
      ggtgaaaaa gccgatgct tgtatgatt aatcggaaa 
      gtgtcgcat gatcagttt gatgctgcc tgtgatgat 
      ttgtatagg ggtatcgag gaggctttg tccaagctt 
      gaggaacaa gaggaaatg tggcaccaa gccctcgac 
      tcgatccag cgcgaagtt ggcaataaa cttgacaaa 
      atggaagtg gatccatta cgcgacttt gtcaacagt 
      aaactaaga gcactgcaa gagaaattc aagtcgtta 
      agtgccctc aaacgagag caggaagct gcaggaaca 
      aagagtcga tttttgcga aatgttaat tgtatttcc 
      tgtgataag gacgtaata atgaggaaa gaaatggat 
      gcctcttta aaacccaaa gcctatgca atgccagct 
      agtagaaat atggcacct tatctagct tatgaattg 
      gaccagttg agaaagcaa caaaaatgt gtccccaat 
      agcaagaat ctgaacttt attgaaagt gccttgaaa 
      acacgagga aataaagag aaaaatcat ttatgtaac 
      cggtattgt ggagggagt catactacg accaccccc 
      caacagagg gtgacacga ttggggcat ttcctggaa 
      cagtggggt cctgaaata gccccctta caggatgaa 
      tacattcga gggaacgac aatcatatg tataaagct 
      cgtgacgag gctatttat acgaaaaaa gggagtaaa 
      gttgaaaat gggcctaag gggagcata aggccctct 
      acagcagga ggggcaccc atgatggaa caggaagtt 
      ccccaaggg aggaacact tacgtgagt gctgaagct 
      cacatggtt gtgagtaaa gaagccgaa gaagcagca 
      aataagaag tcgcttaat attggagct gtgcctatt 
      gaggaggtt actcaagcc tctcaacaa ataccagtc 
      aaggtctca attgaggaa ccagtgtct caaaaggcg 
      tcacagttg ggtaaagct gcgtctcaa ctttcgaat 
      gagcagcta aggaaaaca caaagtgcg gaagggaaa 
      agctcgcaa ctttcgggt gagagaaaa tcagcaacg 
      atagaaggg aaaagttcg caactttcc ggtgagaga 
      aaatcggcg actccagaa gggaaaagc tcgcaactt 
      tccggtgaa agaaaatca gcagcgata gaagggaaa 
      agttcgcag ctttctaat gaacagaag aggtcccaa 
      agtgccatt gtggggaaa aaggggtcg gagcaagcc 
      gtggaaaag ggacaagag taa
    mRNA sequence
    • >TC005359 RA
      atcccttcg aaccatcag attcgtgca aaatggccg 
      aaagcgtta cacttgtaa cactctcac aaatgatcg 
      atttatcgt taaattgca acgagggca ttatcaact 
      tccacgtcc tccacacct tcctccacg tcgttgtcc 
      aacaactaa aaatgcacg acgtagctg tggaattcc 
      gaggctccg acagcgaaa aaatccaag ccttgatca 
      gtaccgtca aacctacct ccacccacc tcctcccaa 
      aatacaacg tcgtgcctg aagaaagcg tcaagttga 
      agaaaaaaa caggacccc atcagaaga aaaaagcca 
      gtttacagg acgtgatgg cggaaaaag agcgccaga 
      agttggaag agaaaaaag agaaggaaa tccgtgtta 
      tcgaacgcc ggaaagact caaaggaag accctgacc 
      ttaaccccc tcccgttga aatcacccc caggtagcc 
      tccttggaa tcactcctg ccgacctcc agacggttg 
      tcgtggtcg agtcccagt caaccctcc cccagtaca 
      ctgtggccc tcaccaaaa ccaccttcg acagcctcc 
      aaaatgacc tagactcgc tcaaaatcc aactcaagg 
      agttacaag aactcccag ctaatctgg gcttaattg 
      gggccacga ggtcccagt caagcccga tcctggaca 
      tgttccaaa tcctccact tgaccaaac gaatggacg 
      ccaatgaga cagcccttg agaaaatcg gttcgttga 
      ttgaagact tggtcaaga gcgacaaag ggcagatgc 
      ccccaacca tcatcgaaa gggtccaag aagtgcgcg 
      cttcagtcg acgaaaccc aaaaataca cagctgaac 
      ggcggttaa gtgcgttaa acgaggtcg aagtcaggc 
      taactaacc ttgaaaatg ctgtcaata acttgaatg 
      agaatatag cagctgtgg ccacaggag tcggcgaat 
      ttaaaccgc aagagccaa ttttggaaa aaccagcgt 
      cggctcagc ccagtttgg ggccctctg atatggcca 
      gcgttgcca gaactgaga ctagtagtg tggtccccg 
      aaggggcca aatctccct cgaggaaaa gccaaggga 
      cggagtcgg tcaaaatca tggccccgc aggtggtct 
      accagagcc agatccaag atgtcgatt tgggtgagt 
      tgccctcga accaggccc taatcctga tccaggagg 
      agttccaaa atttgaaga actggatga ctgagagta 
      tccaagaag tggacaagt tgaaagctt tcgctccgc 
      cagcgcctg gcgaagttg acacgcgag ttgttgaca 
      gcaaattca acgaatgta gtcggaaaa tcaacgaac 
      tgaacgact tatacaaca cgcagatca agctcctca 
      gtgagcagt tcaacagcg tccagagcg aaatgaacg 
      aagttgcca ccaaacttg acataattc tcccgggag 
      gtgattcga ccgggatta gtgccgaga cgaccatgg 
      atttggcca ataaactca cgctgcttg agaaggaga 
      tgattgcaa tttcggata aggccgaag agttgcaac 
      aggagaagg aacagcgtg ataagcaga ctgaagtga 
      ttatggaac agattgata ttttgaaaa ttgtgaaag 
      cgaatcggg aagatttgg aggatgcgt tgggtgaaa 
      aagccgatg cttgtatga ttaatcgga aagtgtcgc 
      atgatcagt ttgatgctg cctgtgatg atttgtata 
      ggggtatcg aggaggctt tgtccaagc ttgaggaac 
      aagaggaaa tgtggcacc aagccctcg actcgatcc 
      agcgcgaag ttggcaata aacttgaca aaatggaag 
      tggatccat tacgcgact ttgtcaaca gtaaactaa 
      gagcactgc aagagaaat tcaagtcgt taagtgccc 
      tcaaacgag agcaggaag ctgcaggaa caaagagtc 
      gatttttgc gaaatgtta attgtattt cctgtgata 
      aggacgtaa taatgagga aagaaatgg atgcctctt 
      taaaaccca aagcctatg caatgccag ctagtagaa 
      atatggcac cttatctag cttatgaat tggaccagt 
      tgagaaagc aacaaaaat gtgtcccca atagcaaga 
      atctgaact ttattgaaa gtgccttga aaacacgag 
      gaaataaag agaaaaatc atttatgta accggtatt 
      gtggaggga gtcatacta cgaccaccc cccaacaga 
      gggtgacac gattggggc atttcctgg aacagtggg 
      gtcctgaaa tagccccct tacaggatg aatacattc 
      gagggaacg acaatcata tgtataaag ctcgtgacg 
      aggctattt atacgaaaa aagggagta aagttgaaa 
      atgggccta aggggagca taaggccct ctacagcag 
      gaggggcac ccatgatgg aacaggaag ttccccaag 
      ggaggaaca cttacgtga gtgctgaag ctcacatgg 
      ttgtgagta aagaagccg aagaagcag caaataaga 
      agtcgctta atattggag ctgtgccta ttgaggagg 
      ttactcaag cctctcaac aaataccag tcaaggtct 
      caattgagg aaccagtgt ctcaaaagg cgtcacagt 
      tgggtaaag ctgcgtctc aactttcga atgagcagc 
      taaggaaaa cacaaagtg cggaaggga aaagctcgc 
      aactttcgg gtgagagaa aatcagcaa cgatagaag 
      ggaaaagtt cgcaacttt ccggtgaga gaaaatcgg 
      cgactccag aagggaaaa gctcgcaac tttccggtg 
      aaagaaaat cagcagcga tagaaggga aaagttcgc 
      agctttcta atgaacaga agaggtccc aaagtgcca 
      ttgtgggga aaaaggggt cggagcaag ccgtggaaa 
      agggacaag agtaattta gaagtctaa tggtttaaa 
      tttttgtca agtcattta ttgtggacg tttttaaac 
      aaaataata aaattttta gtgcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY