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Gene information
General information
TC005351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid58: 970429..972653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005351
    iBeetle screen iB_03942
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    only three eggs on the slide
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005351 RA
      MLTIILSVV LGALIYYKL IKPASFWDE RNITHASAW 
      PLIGSMWES VIQKKNFNE ISSDFYKKY PNDRYVGYM 
      QFSNPVLLI RDLDLIKQI GVKVFDNFH DHLAITGIK 
      TDPLFSKSL IVLRGEEWK QMRATLSPA FTSSKMKNM 
      YHLIYECAE NFAKHFQDE KKNVEVKDI FSKFTNDVI 
      ATVAFGIQI NSLKEPNNE FYSMGKLLT TFKGFELMK 
      FFFALALPQ IFELTKLSL FNQKVTNFF RNIIIDNMH 
      KREKDGIVR PDLIHLLME ARKGKLKHD NSNEGGDGF 
      ATVEESEIG KNSKKMELT DDLMVAQAL LFFFAGFET 
      ISTGFSFMA YELATNPDV QKKLQKEID LTLQENHGK 
      ISYNVLQSM KYLDQVVCE SLRLWPPAP QTDRFCNTD 
      FVLEPTKPH ERRFTIERG VTIIIPIYG IHRDPEYFP 
      NPDKFDPER FSDENKAKI VPGTYMPFG VGPRNCIGS 
      RLALLELKT LFFHLLSKC DIVTNSKTV IPLKISPNN 
      GNITPKEGI HLTFKPRNV A
    CDS sequence
    • >TC005351 RA
      atgttgact ataatccta agtgtggtt ttgggtgcg 
      ctcatttat tacaaatta ataaaaccg gccagtttt 
      tgggacgaa agaaacatc actcacgct tcagcatgg 
      cctctgatt ggaagtatg tgggaatcg gttattcaa 
      aagaagaat ttcaatgaa atatctagc gatttctac 
      aagaaatac ccaaacgat cgatacgtg ggttacatg 
      caattttca aacccagtt ctcctaatc agagacttg 
      gacttgatt aaacaaatt ggagtgaag gtctttgat 
      aattttcac gaccatctt gccataact ggtattaaa 
      actgatcct ttgttcagt aaaagtcta attgtttta 
      cgaggagag gaatggaag caaatgagg gcgacttta 
      agcccggct ttcactagc agcaaaatg aaaaatatg 
      tatcacttg atttacgaa tgtgccgaa aatttcgcc 
      aaacatttt caagacgag aaaaaaaat gtcgaagtg 
      aaggatatt ttttcgaaa tttaccaac gatgtgatc 
      gcaaccgta gcttttgga attcaaatt aattctcta 
      aaggagcca aacaacgag ttttactcg atgggaaaa 
      ttactgact acgtttaaa ggttttgaa ctaatgaag 
      ttttttttt gccctagca cttccccaa atttttgag 
      cttaccaaa ttaagttta ttcaatcaa aaagttaca 
      aacttcttc agaaatatt attattgac aatatgcac 
      aaacgtgaa aaagacgga attgttcgg cccgatttg 
      attcattta ttaatggaa gcccgtaaa ggcaaactt 
      aaacatgat aacagtaat gaaggtggt gatggtttt 
      gccacagtt gaagagtcg gaaattggc aaaaattcc 
      aaaaaaatg gaactaaca gacgaccta atggttgct 
      caagctttg ctcttcttt ttcgctggt tttgaaacg 
      atttcgacc ggtttcagt ttcatggct tatgaattg 
      gccacaaat cctgacgtt cagaagaaa ctacaaaag 
      gaaatagac ttgacactg caagaaaat cacggaaaa 
      atttcgtac aacgtgcta caatcgatg aaatatctg 
      gaccaagtt gtgtgtgaa agtttacga ctttggcca 
      ccggctccc caaaccgat cgtttctgc aacaccgac 
      tttgttctt gaaccaaca aaaccacac gaaagacgg 
      tttacgatt gaaagaggt gttacaata attatcccg 
      atttatgga atacataga gaccctgag tactttcca 
      aatccggac aaattcgat cctgagcgg tttagtgac 
      gaaaataag gccaaaatt gtgccaggg acttatatg 
      ccgtttggt gtcggtcca agaaattgc attggttcg 
      aggcttgct ttgttggaa ctgaaaacg ctattcttc 
      cacttgtta tcaaaatgt gatattgtg acgaacagc 
      aagacggta attccgctt aaaatcagc ccaaataat 
      gggaatatt acgccaaaa gagggaatc catctaaca 
      ttcaagccc agaaacgtg gcctaa
    mRNA sequence
    • >TC005351 RA
      agtgatgag atgattcag aaaactcaa atgactgtc 
      tattaaaaa tcatcataa tactaaaaa ctcgttagc 
      tacgaaata attttattt tcctgcttt ttttgaatc 
      aatgtactt cacatattt tttatgtta attaattag 
      tatattttt caaaataat aatttattt ttgatgacg 
      ctttcatac ttttttgtt tttttgtaa aattcataa 
      tttgtgatt ttacgccaa aaaatcaag tcattgatg 
      acaagtatt tttaaatga ttggaaatg ctcttaact 
      ctttatctt tctaaatag cttatgaca actcactgt 
      aaataaaca actcgcttg gtgggagaa tcagtctct 
      accaacatg ttgactata atcctaagt gtggttttg 
      ggtgcgctc atttattac aaattaata aaaccggcc 
      agtttttgg gacgaaaga aacatcact cacgcttca 
      gcatggcct ctgattgga agtatgtgg gaatcggtt 
      attcaaaag aagaatttc aatgaaata tctagcgat 
      ttctacaag aaataccca aacgatcga tacgtgggt 
      tacatgcaa ttttcaaac ccagttctc ctaatcaga 
      gacttggac ttgattaaa caaattgga gtgaaggtc 
      tttgataat tttcacgac catcttgcc ataactggt 
      attaaaact gatcctttg ttcagtaaa agtctaatt 
      gttttacga ggagaggaa tggaagcaa atgagggcg 
      actttaagc ccggctttc actagcagc aaaatgaaa 
      aatatgtat cacttgatt tacgaatgt gccgaaaat 
      ttcgccaaa cattttcaa gacgagaaa aaaaatgtc 
      gaagtgaag gatattttt tcgaaattt accaacgat 
      gtgatcgca accgtagct tttggaatt caaattaat 
      tctctaaag gagccaaac aacgagttt tactcgatg 
      ggaaaatta ctgactacg tttaaaggt tttgaacta 
      atgaagttt ttttttgcc ctagcactt ccccaaatt 
      tttgagctt accaaatta agtttattc aatcaaaaa 
      gttacaaac ttcttcaga aatattatt attgacaat 
      atgcacaaa cgtgaaaaa gacggaatt gttcggccc 
      gatttgatt catttatta atggaagcc cgtaaaggc 
      aaacttaaa catgataac agtaatgaa ggtggtgat 
      ggttttgcc acagttgaa gagtcggaa attggcaaa 
      aattccaaa aaaatggaa ctaacagac gacctaatg 
      gttgctcaa gctttgctc ttctttttc gctggtttt 
      gaaacgatt tcgaccggt ttcagtttc atggcttat 
      gaattggcc acaaatcct gacgttcag aagaaacta 
      caaaaggaa atagacttg acactgcaa gaaaatcac 
      ggaaaaatt tcgtacaac gtgctacaa tcgatgaaa 
      tatctggac caagttgtg tgtgaaagt ttacgactt 
      tggccaccg gctccccaa accgatcgt ttctgcaac 
      accgacttt gttcttgaa ccaacaaaa ccacacgaa 
      agacggttt acgattgaa agaggtgtt acaataatt 
      atcccgatt tatggaata catagagac cctgagtac 
      tttccaaat ccggacaaa ttcgatcct gagcggttt 
      agtgacgaa aataaggcc aaaattgtg ccagggact 
      tatatgccg tttggtgtc ggtccaaga aattgcatt 
      ggttcgagg cttgctttg ttggaactg aaaacgcta 
      ttcttccac ttgttatca aaatgtgat attgtgacg 
      aacagcaag acggtaatt ccgcttaaa atcagccca 
      aataatggg aatattacg ccaaaagag ggaatccat 
      ctaacattc aagcccaga aacgtggcc taattaaaa 
      gaccgttcc ataaattta taaatcaat tgtaacaaa 
      tgtaataaa aataaataa aatttttat aagttc