Status
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Gene information
General information
TC005347 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14196382..14199538
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005347
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03941

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005347 RA
      MIITRVVQN PTVQSLFRR LSLSHADLH NISNNFQAT 
      ASTLPRLRD HEKKPTLKP LLRSMSLGP NLARTMSTS 
      TKTNRLALE KSPYLLQHA TNPVDWYPW GQEAFDRAK 
      KENKLIFLS VGYSTCHWC HVMEKESFE DEEVAKIMN 
      QHFINVKVD REERPDVDK LYMAFIQAS VGGGGWPMS 
      VFLTPTLEP LAGGTYFPP EDKYGRPGF KTVLKSIAE 
      QWRTKQSAI ANSGKYSLE VLRKVSERE ISAKQDINV 
      PGEDVWKKC LLQLSHSYE DDFGGFSAQ PKFPQPCNL 
      NFLFHMYSR DKHSEQGFR CLHMCLNTL RKMAYGGIH 
      DHVNCGFAR YSVDDRWHV PHFEKMLYD QAQLAVSYA 
      DAFVVTKDD FFAEVLRDI LLYVSRDLS HPLGGFYGA 
      EDADSYPYE GASHKREGA FCVWEFEEI SKLLGETKT 
      DDISHRDLF IYHYNVKED GNVNPAQDP HHELEKKNI 
      LVCFGSFED TSRKFKTSV ETVKEILKS CHEILYKER 
      QKRPKPHVD TKIVTSWNG LMISGFAKA GFVLKDQEY 
      INRAILAAT FIKKFLYNE QDKTLLRCC YKGDNAKIV 
      QTPTPVNGF LDDYAFLIR GLLDLYEAS LDADWLSWA 
      EVLQEQQDR LFWDTKGSG YFTSPANDS SILIRGKED 
      QDGAEPCGN SIAVHNLIR LAAYLDRAD LRAKAGRTL 
      TVFADRLKS IPVALPEMT SALLFYHNS PTQVFIAGP 
      TEDNNTQAL IDVVRSRFI PGRILAVTD GPGGLLYRR 
      HESLARLRP IQGKPAAYV CRNFACSLP VTEPEELAS 
      NLDGNLAEN PDE
    CDS sequence
    • >TC005347 RA
      atgattata acgcgtgtc gtgcaaaac ccaaccgtc 
      cagagcctg tttcgcaga ctgtccttg tcacatgca 
      gatttgcat aatatttca aacaacttt caagcgacc 
      gcttccaca ctccccaga ttacgcgac cacgagaaa 
      aaaccaact ttgaagcca cttcttagg tcaatgtca 
      ttgggccca aacctcgct cgaacaatg tcaacttcg 
      accaaaaca aatcgactg gcattggaa aaatctccg 
      tatttgcta caacatgcc actaacccc gtcgattgg 
      tatccctgg ggccaggag gccttcgat cgcgcaaaa 
      aaggaaaac aaactcatt ttcctctca gttggctac 
      tccacttgc cactggtgt cacgtgatg gagaaagag 
      tcgtttgaa gatgaagag gtggcaaaa atcatgaac 
      caacacttt atcaatgtt aaagtggac cgcgaggag 
      cgtcctgac gttgataaa ctctacatg gcgtttatt 
      caggctagt gttgggggt gggggctgg ccaatgtct 
      gtgttcctc accccaaca cttgagccc ttagctggt 
      ggtacgtac ttcccccct gaggataag tacggacga 
      cctggcttt aaaaccgtg ttgaaatca atcgccgag 
      caatggcgg accaaacag tcggccatt gccaactcg 
      gggaagtac tcattagag gtgttacgt aaagtgagc 
      gagcgggaa atttccgcc aaacaggat attaacgtt 
      ccgggtgag gacgtttgg aagaagtgt cttctccaa 
      ttgtcacac tcttacgag gatgatttt gggggattt 
      tcagcacaa ccgaaattc ccccaaccg tgtaacttg 
      aattttttg tttcacatg tattcgaga gataaacac 
      tcagaacag gggtttagg tgcctccat atgtgtttg 
      aacacactg agaaaaatg gcatatggt ggtatacat 
      gatcatgtt aattgtggg tttgccaga tattcggtt 
      gatgaccgt tggcatgtc ccacatttc gaaaaaatg 
      ctttacgat caagcacaa ttggcggtt tcttacgcc 
      gatgcgttt gttgttacc aaagacgat ttctttgct 
      gaggtcctc agagacatc ctcttgtat gtctcgagg 
      gatttgagt cacccttta ggcgggttc tatggggct 
      gaagatgcc gattcctac ccttatgaa ggggcttcc 
      cataaacgc gaaggtgcg ttttgtgtt tgggagttt 
      gaagaaatc tcaaaactt ctcggagaa actaaaacc 
      gacgatata tctcatcgt gatttgttc atctatcat 
      tacaatgtc aaagaagat ggtaatgtt aacccagcg 
      caagacccg catcatgaa ttggaaaaa aagaacatt 
      ttggtttgt ttcgggtcg tttgaagac acgtcaaga 
      aagtttaag acgtcggtg gaaaccgtt aaagagatt 
      ttgaaaagt tgccacgaa attttgtac aaggagaga 
      caaaaaagg ccaaaaccg cacgttgat actaagatt 
      gtaacgtcc tggaacggg ttaatgatc tccgggttt 
      gctaaagct gggtttgtt cttaaagat caagagtat 
      ataaatcgg gccatcctg gcggccact ttcattaaa 
      aagtttctc tacaatgag caagataag acactgttg 
      cggtgttgc tataaggga gacaacgcc aaaatcgtc 
      caaacacca acccctgtc aacggtttt ttggacgac 
      tacgccttc ttgattcgg ggccttctc gatttatac 
      gaggcatct ctcgatgct gattggtta tcgtgggcg 
      gaagtgttg caagagcaa caagatcgc ctcttttgg 
      gacactaaa ggttccggt tatttcaca agtcctgca 
      aacgactcg agtatattg attcggggc aaggaagat 
      caagacggt gctgagccc tgtggcaac tcgattgcc 
      gttcacaat ttaatccga ctcgccgcc tacttggac 
      cgggcggat ttgagggca aaggccggt cggacgttg 
      acagtcttt gccgatcgg ttaaaatcg attccggtg 
      gctttgcct gaaatgact tcggctttg ctcttttat 
      cataattcg ccgacacag gtttttata gccggaccg 
      acggaggat aacaacact caggctttg attgacgtg 
      gtcaggtct aggtttata cctgggagg attttggcg 
      gtgactgat ggaccagga ggattgttg taccgccgg 
      catgaatcc ttggctagg cttcggccg attcaaggc 
      aaacctgcg gcgtatgtt tgccgaaat ttcgcgtgt 
      tcgttaccg gttaccgaa ccggaggaa ttggccagt 
      aacctggac gggaatttg gccgaaaat cccgacgaa 
      taa
    mRNA sequence
    • >TC005347 RA
      agccaaacc aaaatattc cttcctttc acttttcta 
      catttaaaa ataatcaaa cgtcgcatt tcttctatt 
      ttgctcgta ccatgtagt accccaagc accaccgcg 
      atgattata acgcgtgtc gtgcaaaac ccaaccgtc 
      cagagcctg tttcgcaga ctgtccttg tcacatgca 
      gatttgcat aatatttca aacaacttt caagcgacc 
      gcttccaca ctccccaga ttacgcgac cacgagaaa 
      aaaccaact ttgaagcca cttcttagg tcaatgtca 
      ttgggccca aacctcgct cgaacaatg tcaacttcg 
      accaaaaca aatcgactg gcattggaa aaatctccg 
      tatttgcta caacatgcc actaacccc gtcgattgg 
      tatccctgg ggccaggag gccttcgat cgcgcaaaa 
      aaggaaaac aaactcatt ttcctctca gttggctac 
      tccacttgc cactggtgt cacgtgatg gagaaagag 
      tcgtttgaa gatgaagag gtggcaaaa atcatgaac 
      caacacttt atcaatgtt aaagtggac cgcgaggag 
      cgtcctgac gttgataaa ctctacatg gcgtttatt 
      caggctagt gttgggggt gggggctgg ccaatgtct 
      gtgttcctc accccaaca cttgagccc ttagctggt 
      ggtacgtac ttcccccct gaggataag tacggacga 
      cctggcttt aaaaccgtg ttgaaatca atcgccgag 
      caatggcgg accaaacag tcggccatt gccaactcg 
      gggaagtac tcattagag gtgttacgt aaagtgagc 
      gagcgggaa atttccgcc aaacaggat attaacgtt 
      ccgggtgag gacgtttgg aagaagtgt cttctccaa 
      ttgtcacac tcttacgag gatgatttt gggggattt 
      tcagcacaa ccgaaattc ccccaaccg tgtaacttg 
      aattttttg tttcacatg tattcgaga gataaacac 
      tcagaacag gggtttagg tgcctccat atgtgtttg 
      aacacactg agaaaaatg gcatatggt ggtatacat 
      gatcatgtt aattgtggg tttgccaga tattcggtt 
      gatgaccgt tggcatgtc ccacatttc gaaaaaatg 
      ctttacgat caagcacaa ttggcggtt tcttacgcc 
      gatgcgttt gttgttacc aaagacgat ttctttgct 
      gaggtcctc agagacatc ctcttgtat gtctcgagg 
      gatttgagt cacccttta ggcgggttc tatggggct 
      gaagatgcc gattcctac ccttatgaa ggggcttcc 
      cataaacgc gaaggtgcg ttttgtgtt tgggagttt 
      gaagaaatc tcaaaactt ctcggagaa actaaaacc 
      gacgatata tctcatcgt gatttgttc atctatcat 
      tacaatgtc aaagaagat ggtaatgtt aacccagcg 
      caagacccg catcatgaa ttggaaaaa aagaacatt 
      ttggtttgt ttcgggtcg tttgaagac acgtcaaga 
      aagtttaag acgtcggtg gaaaccgtt aaagagatt 
      ttgaaaagt tgccacgaa attttgtac aaggagaga 
      caaaaaagg ccaaaaccg cacgttgat actaagatt 
      gtaacgtcc tggaacggg ttaatgatc tccgggttt 
      gctaaagct gggtttgtt cttaaagat caagagtat 
      ataaatcgg gccatcctg gcggccact ttcattaaa 
      aagtttctc tacaatgag caagataag acactgttg 
      cggtgttgc tataaggga gacaacgcc aaaatcgtc 
      caaacacca acccctgtc aacggtttt ttggacgac 
      tacgccttc ttgattcgg ggccttctc gatttatac 
      gaggcatct ctcgatgct gattggtta tcgtgggcg 
      gaagtgttg caagagcaa caagatcgc ctcttttgg 
      gacactaaa ggttccggt tatttcaca agtcctgca 
      aacgactcg agtatattg attcggggc aaggaagat 
      caagacggt gctgagccc tgtggcaac tcgattgcc 
      gttcacaat ttaatccga ctcgccgcc tacttggac 
      cgggcggat ttgagggca aaggccggt cggacgttg 
      acagtcttt gccgatcgg ttaaaatcg attccggtg 
      gctttgcct gaaatgact tcggctttg ctcttttat 
      cataattcg ccgacacag gtttttata gccggaccg 
      acggaggat aacaacact caggctttg attgacgtg 
      gtcaggtct aggtttata cctgggagg attttggcg 
      gtgactgat ggaccagga ggattgttg taccgccgg 
      catgaatcc ttggctagg cttcggccg attcaaggc 
      aaacctgcg gcgtatgtt tgccgaaat ttcgcgtgt 
      tcgttaccg gttaccgaa ccggaggaa ttggccagt 
      aacctggac gggaatttg gccgaaaat cccgacgaa 
      taaattttc agtttgtta tatactgtt attaaataa 
      aaaagcgac ctgtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY