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Gene information
General information
TC005341 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14312241..14324998
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005341
    iBeetle screen iB_03939

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    dorsal thoracic muscles structure thinner - Number of animals showing the phenotype: 7

    Adult morphology
    elytra orientation not closed - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005341 RA
      MAVRMDSTQ TPNRKINYK VTNSNGGGG GARTASFGG 
      VKGGGGGGA PMATQHQTT GAYRQWGGV GGGHQHGHP 
      TTGSTTYYQ RYPVSQTAT AASTPASAV TQQQQQQAS 
      YTPSTTTTY NSSNSYGNQ RVPTATSPS NTSSSSSHT 
      GSQSGTVST SVSNNMPNN TNSSPNSSS GSEQLSKTN 
      LYIRGLNPT TTDKDLVNM CQQFGTIIS TKAILDKTT 
      NKCKGYGFV DFESPSAAE GAVKALTAN NIQAQMAKQ 
      QEQDPTNLY IANLPSHFK ESDLDNLLS KYGQVISTR 
      ILRDSMGIS KGVGFARME NKDKCEQII TIFNGNTLT 
      GCKEPLLVK FADGGNKKK NMYKNNENA KMWRDGTEG 
      MAVAGYDPN TLAQNGVAA QHMIPAAIS NFRAPYGQY 
      AGYAPGAQW VPQYVMPAP MPTVDDSYN IPGHMAPYK 
      SDGQGPRGI PMMLPSPEA AVQYANVIP QLTAQMQAM 
      QLGTGSYIS QPYSYYPSH VIHTVPMGD SEHTSNAAS 
      PEDPYQAYP PAPPK
    • >TC005341 RB
      MATQHQTTG AYRQWGGVG GGHQHGHPT TGSTTYYQR 
      YPVSQTATA ASTPASAVT QQQQQQASY TPSTTTTYN 
      SSNSYGNQR VPTATSPSN TSSSSSHTG SQSGTVSTS 
      VSNNMPNNT NSSPNSSSG SEQLSKTNL YIRGLNPTT 
      TDKDLVNMC QQFGTIIST KAILDKTTN KCKGYGFVD 
      FESPSAAEG AVKALTANN IQAQMAKQQ EQDPTNLYI 
      ANLPSHFKE SDLDNLLSK YGQVISTRI LRDSMGISK 
      GVGFARMEN KDKCEQIIT IFNGNTLTG CKEPLLVKF 
      ADGGNKKKN MYKNNENAK MWRDGTEGM AVAGYDPNT 
      LAQNGVAAQ HMIPAAISN FRAPYGQYA GYAPGAQWV 
      PQYVMPAPM PTVDDSYNI PGHMAPYKS DGQGPRGIP 
      MMLPSPEAA VQYANVIPQ LTAQMQAMQ LGTGSYISQ 
      PYSYYPSHV IHTVPMGDS EHTSNAASP EDPYQAYPP 
      APPK
    CDS sequence
    • >TC005341 RA
      atggccgtt cgtatggat agcacccag acacctaat 
      aggaaaatc aactataaa gtcacgaat agtaacgga 
      ggcggcggc ggagcgagg acagcgtcg ttcggcggc 
      gtgaagggc ggcggtggt ggcggtgcg cccatggcc 
      acccagcac caaacaacg ggggcctac cgccagtgg 
      ggcggcgtc ggcggcggc caccagcac ggccatcca 
      acaacagga agcaccact tactaccag agatatccg 
      gtgagtcaa acggcgacc gcagcttcg acgccggct 
      tcggccgtc acacaacag caacaacag caggcctcc 
      tacacacct tcaaccacc accacctac aattcgtcg 
      aattcgtac ggaaatcag agagtgcca acggccacg 
      tcgccatcg aacacgagt agctctagt agccacacg 
      ggcagtcaa agcggcact gtatccact tctgttagt 
      aacaacatg cccaacaat acgaactca tcgccaaac 
      agttcttcc gggtcagaa caactctcc aaaacgaat 
      ttgtacata agaggactg aatccgacc accactgac 
      aaggatctt gtcaacatg tgccagcag ttcggtacc 
      attatatcc accaaagcc atattggac aagacgaca 
      aataaatgt aaaggttat ggatttgtt gattttgaa 
      agtccgtca gcagcagag ggtgcagtc aaggctctt 
      actgcaaat aacattcag gctcaaatg gcgaaacaa 
      caagagcaa gaccccaca aatttgtac atagctaat 
      ctgccctct cattttaaa gaatcagac ttggacaat 
      ctgttgtcg aaatacggt caagtgata tcgacgagg 
      attcttcgc gattcgatg ggaatttcg aaaggtgtc 
      ggttttgca cgcatggaa aacaaagat aaatgcgag 
      caaattata acgatattt aatggaaat acgttgacc 
      ggttgtaaa gaacccctt ttggtcaag tttgccgac 
      ggtggtaac aagaagaaa aatatgtat aagaataac 
      gaaaatgcg aaaatgtgg cgcgatggg actgaagga 
      atggccgtt gctggatat gatcctaat acgttggcg 
      caaaacggc gtcgcggcg caacatatg ataccggcg 
      gctatatcg aactttagg gcaccctac ggacagtac 
      gccggctac gcccccggt gcccagtgg gtgccccaa 
      tacgtaatg ccggcccca atgcccacc gtggacgac 
      agctacaac atccctggc catatggcg ccgtacaag 
      agcgacggt caaggtcct cgaggcatc ccaatgatg 
      ttaccgtca ccggaggcg gccgttcag tacgccaac 
      gtcatacct cagttgact gctcaaatg caggccatg 
      caattaggc accggttca tacatctca caaccatac 
      tcgtactat ccgtcacat gtcatccat accgtacca 
      atgggtgac tccgaacac acatctaat gcggctagt 
      cccgaagat ccatatcag gcatacccg cctgcacca 
      cctaaataa
    • >TC005341 RB
      atggccacc cagcaccaa acaacgggg gcctaccgc 
      cagtggggc ggcgtcggc ggcggccac cagcacggc 
      catccaaca acaggaagc accacttac taccagaga 
      tatccggtg agtcaaacg gcgaccgca gcttcgacg 
      ccggcttcg gccgtcaca caacagcaa caacagcag 
      gcctcctac acaccttca accaccacc acctacaat 
      tcgtcgaat tcgtacgga aatcagaga gtgccaacg 
      gccacgtcg ccatcgaac acgagtagc tctagtagc 
      cacacgggc agtcaaagc ggcactgta tccacttct 
      gttagtaac aacatgccc aacaatacg aactcatcg 
      ccaaacagt tcttccggg tcagaacaa ctctccaaa 
      acgaatttg tacataaga ggactgaat ccgaccacc 
      actgacaag gatcttgtc aacatgtgc cagcagttc 
      ggtaccatt atatccacc aaagccata ttggacaag 
      acgacaaat aaatgtaaa ggttatgga tttgttgat 
      tttgaaagt ccgtcagca gcagagggt gcagtcaag 
      gctcttact gcaaataac attcaggct caaatggcg 
      aaacaacaa gagcaagac cccacaaat ttgtacata 
      gctaatctg ccctctcat tttaaagaa tcagacttg 
      gacaatctg ttgtcgaaa tacggtcaa gtgatatcg 
      acgaggatt cttcgcgat tcgatggga atttcgaaa 
      ggtgtcggt tttgcacgc atggaaaac aaagataaa 
      tgcgagcaa attataacg atatttaat ggaaatacg 
      ttgaccggt tgtaaagaa ccccttttg gtcaagttt 
      gccgacggt ggtaacaag aagaaaaat atgtataag 
      aataacgaa aatgcgaaa atgtggcgc gatgggact 
      gaaggaatg gccgttgct ggatatgat cctaatacg 
      ttggcgcaa aacggcgtc gcggcgcaa catatgata 
      ccggcggct atatcgaac tttagggca ccctacgga 
      cagtacgcc ggctacgcc cccggtgcc cagtgggtg 
      ccccaatac gtaatgccg gccccaatg cccaccgtg 
      gacgacagc tacaacatc cctggccat atggcgccg 
      tacaagagc gacggtcaa ggtcctcga ggcatccca 
      atgatgtta ccgtcaccg gaggcggcc gttcagtac 
      gccaacgtc atacctcag ttgactgct caaatgcag 
      gccatgcaa ttaggcacc ggttcatac atctcacaa 
      ccatactcg tactatccg tcacatgtc atccatacc 
      gtaccaatg ggtgactcc gaacacaca tctaatgcg 
      gctagtccc gaagatcca tatcaggca tacccgcct 
      gcaccacct aaataa
    mRNA sequence
    • >TC005341 RA
      gggcgccat gtgttcggt ggcgctctc gcggagttt 
      cggcgaaac cgatcgcga gacgaccga ccgacacca 
      ccgtcgtca accaagatg gtgtccgcg tagcgcaca 
      gcgcctact atccgaggg gagtggcga tagacgacg 
      aagagagtg tgcggcgtc ggagacggt gttgttact 
      ggtggtgtc gttgggaca ccaagtccc atctctaaa 
      agggggggc cctccggta attgacaaa atttacacc 
      acctaccaa caaaaataa taaaaaaag ataaaaatc 
      gtacctcct ccaagtgcc gtgcggcga cgatgacga 
      ccaccgtca tcatagtgt gccatgaca caacatcat 
      cacccaacg aataaaaga cacacccct acgcgagat 
      ccatcaaca acatcaact catctgtga tataataat 
      tactatggc cgttcgtat ggatagcac ccagacacc 
      taataggaa aatcaacta taaagtcac gaatagtaa 
      cggaggcgg cggcggagc gaggacagc gtcgttcgg 
      cggcgtgaa gggcggcgg tggtggcgg tgcgcccat 
      ggccaccca gcaccaaac aacgggggc ctaccgcca 
      gtggggcgg cgtcggcgg cggccacca gcacggcca 
      tccaacaac aggaagcac cacttacta ccagagata 
      tccggtgag tcaaacggc gaccgcagc ttcgacgcc 
      ggcttcggc cgtcacaca acagcaaca acagcaggc 
      ctcctacac accttcaac caccaccac ctacaattc 
      gtcgaattc gtacggaaa tcagagagt gccaacggc 
      cacgtcgcc atcgaacac gagtagctc tagtagcca 
      cacgggcag tcaaagcgg cactgtatc cacttctgt 
      tagtaacaa catgcccaa caatacgaa ctcatcgcc 
      aaacagttc ttccgggtc agaacaact ctccaaaac 
      gaatttgta cataagagg actgaatcc gaccaccac 
      tgacaagga tcttgtcaa catgtgcca gcagttcgg 
      taccattat atccaccaa agccatatt ggacaagac 
      gacaaataa atgtaaagg ttatggatt tgttgattt 
      tgaaagtcc gtcagcagc agagggtgc agtcaaggc 
      tcttactgc aaataacat tcaggctca aatggcgaa 
      acaacaaga gcaagaccc cacaaattt gtacatagc 
      taatctgcc ctctcattt taaagaatc agacttgga 
      caatctgtt gtcgaaata cggtcaagt gatatcgac 
      gaggattct tcgcgattc gatgggaat ttcgaaagg 
      tgtcggttt tgcacgcat ggaaaacaa agataaatg 
      cgagcaaat tataacgat atttaatgg aaatacgtt 
      gaccggttg taaagaacc ccttttggt caagtttgc 
      cgacggtgg taacaagaa gaaaaatat gtataagaa 
      taacgaaaa tgcgaaaat gtggcgcga tgggactga 
      aggaatggc cgttgctgg atatgatcc taatacgtt 
      ggcgcaaaa cggcgtcgc ggcgcaaca tatgatacc 
      ggcggctat atcgaactt tagggcacc ctacggaca 
      gtacgccgg ctacgcccc cggtgccca gtgggtgcc 
      ccaatacgt aatgccggc cccaatgcc caccgtgga 
      cgacagcta caacatccc tggccatat ggcgccgta 
      caagagcga cggtcaagg tcctcgagg catcccaat 
      gatgttacc gtcaccgga ggcggccgt tcagtacgc 
      caacgtcat acctcagtt gactgctca aatgcaggc 
      catgcaatt aggcaccgg ttcatacat ctcacaacc 
      atactcgta ctatccgtc acatgtcat ccataccgt 
      accaatggg tgactccga acacacatc taatgcggc 
      tagtcccga agatccata tcaggcata cccgcctgc 
      accacctaa ataagttga aaaaaaaaa aagagaaaa 
      aatacaagt ttttttgct aaagtcaca cgagttgaa 
      tgtacagat tgtacgaat tagcttcta atttaattt 
      tttgttttg ttcttgagt caaatacac tcaaagtgt 
      ttagtttaa cgatcggtg tatttaact gtgttgacc 
      tcttttggt ttgctgctt gatttcggt cacttttca 
      attgttaca attaaaaca aatgactga aaatacgtg 
      taaagtagg tatttttga tacagtatg ttcctagtt 
      tcaaacatt gtattgaaa atatgaatt atctgtgta 
      agaaaacag tggttttta cgtgttaag tttcgcgct 
      ttctgtgag tgtgctgta catagatgt tacgagcgg 
      ttttttttc ttacatgga tagaacaac ataccaaca 
      cagaaaaaa acttagaat ttttagact agtagagac 
      gaagaaaac atcgttttt taaaaaata acaaaggtc 
      tgacggtag atacagcaa gcaaaaccg tatttgatc 
      ttcccaagc caaaacggc ataggttat aggtaaatt 
      agcgatttt attttttga tgaagatct acataggta 
      gttgtgtgc gcaacgcag atgcgataa actgctcat 
      tgaaatcaa ataattctt cctaccatc tatacaata 
      tgaactga
    • >TC005341 RB
      cggcggcgt tcagcagtg tctcaccaa ccttcgagt 
      tttaatcat gtgatctgt gcatgtgtg tcatatgtt 
      tattatatg tgccagtgt tttgttttg tcgcgagga 
      ggcctactc gatccgtcg agggttttt ttcacatgt 
      gatatgtgc cgattgttt aatttttgc agtgtaata 
      aggactaga atagtaacg gaggcggcg gcggagcga 
      ggacagcgt cgttcggcg gcgtgaagg gcggcggtg 
      gtggcggtg cgcccatgg ccacccagc accaaacaa 
      cgggggcct accgccagt ggggcggcg tcggcggcg 
      gccaccagc acggccatc caacaacag gaagcacca 
      cttactacc agagatatc cggtgagtc aaacggcga 
      ccgcagctt cgacgccgg cttcggccg tcacacaac 
      agcaacaac agcaggcct cctacacac cttcaacca 
      ccaccacct acaattcgt cgaattcgt acggaaatc 
      agagagtgc caacggcca cgtcgccat cgaacacga 
      gtagctcta gtagccaca cgggcagtc aaagcggca 
      ctgtatcca cttctgtta gtaacaaca tgcccaaca 
      atacgaact catcgccaa acagttctt ccgggtcag 
      aacaactct ccaaaacga atttgtaca taagaggac 
      tgaatccga ccaccactg acaaggatc ttgtcaaca 
      tgtgccagc agttcggta ccattatat ccaccaaag 
      ccatattgg acaagacga caaataaat gtaaaggtt 
      atggatttg ttgattttg aaagtccgt cagcagcag 
      agggtgcag tcaaggctc ttactgcaa ataacattc 
      aggctcaaa tggcgaaac aacaagagc aagacccca 
      caaatttgt acatagcta atctgccct ctcatttta 
      aagaatcag acttggaca atctgttgt cgaaatacg 
      gtcaagtga tatcgacga ggattcttc gcgattcga 
      tgggaattt cgaaaggtg tcggttttg cacgcatgg 
      aaaacaaag ataaatgcg agcaaatta taacgatat 
      ttaatggaa atacgttga ccggttgta aagaacccc 
      ttttggtca agtttgccg acggtggta acaagaaga 
      aaaatatgt ataagaata acgaaaatg cgaaaatgt 
      ggcgcgatg ggactgaag gaatggccg ttgctggat 
      atgatccta atacgttgg cgcaaaacg gcgtcgcgg 
      cgcaacata tgataccgg cggctatat cgaacttta 
      gggcaccct acggacagt acgccggct acgcccccg 
      gtgcccagt gggtgcccc aatacgtaa tgccggccc 
      caatgccca ccgtggacg acagctaca acatccctg 
      gccatatgg cgccgtaca agagcgacg gtcaaggtc 
      ctcgaggca tcccaatga tgttaccgt caccggagg 
      cggccgttc agtacgcca acgtcatac ctcagttga 
      ctgctcaaa tgcaggcca tgcaattag gcaccggtt 
      catacatct cacaaccat actcgtact atccgtcac 
      atgtcatcc ataccgtac caatgggtg actccgaac 
      acacatcta atgcggcta gtcccgaag atccatatc 
      aggcatacc cgcctgcac cacctaaat aagttgaaa 
      aaaaaaaaa gagaaaaaa tacaagttt ttttgctaa 
      agtcacacg agttgaatg tacagattg tacgaatta 
      gcttctaat ttaattttt tgttttgtt cttgagtca 
      aatacactc aaagtgttt agtttaacg atcggtgta 
      tttaactgt gttgacctc ttttggttt gctgcttga 
      tttcggtca cttttcaat tgttacaat taaaacaaa 
      tgactgaaa atacgtgta aagtaggta tttttgata 
      cagtatgtt cctagtttc aaacattgt attgaaaat 
      atgaattat ctgtgtaag aaaacagtg gtttttacg 
      tgttaagtt tcgcgcttt ctgtgagtg tgctgtaca 
      tagatgtta cgagcggtt ttttttctt acatggata 
      gaacaacat accaacaca gaaaaaaac ttagaattt 
      ttagactag tagagacga agaaaacat cgtttttta 
      aaaaataac aaaggtctg acggtagat acagcaagc 
      aaaaccgta tttgatctt cccaagcca aaacggcat 
      aggttatag gtaaattag cgattttat tttttgatg 
      aagatctac ataggtagt tgtgtgcgc aacgcagat 
      gcgataaac tgctcattg aaatcaaat aattcttcc 
      taccatcta tacaatatg aactga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY