Status
Please wait ...
Gene information
General information
TC034197
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005328,TC005327
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14421567..14432712
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034197
    iBeetle screen iB_03937

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034197 RA
      MSRIILLFV LVTTTTCDD LLDEYVRHV DDVNKPNKV 
      HQRRLRRDI ADKWTHSEV LDNVGDVVL RWQPRHREI 
      LFRVEARTK GYVAIGFSP DGGKENADI VMGWVDDRT 
      LRAYLLDCH GVPDSQGSV PIKDEIDNY TLMSGSQND 
      THTVIEFRR VLDTCDPND YVLTGDTVV VIWAYHNTD 
      PHLGAEMIY HGDKRGAQS LHLLGPPPV SKASGGNIR 
      NWDVTLRNF EIISNMNTV YWCKIFKAP TLQHKHHIV 
      GYEPLIGSN HTTFVHHMI LHECELDGE DVHKWERFS 
      KENGRLCYG SNMAPEWEK CLTPLVAWA IGSKGENFP 
      KHVGLPLAA KKNSFYMLE VHFDNPAMK QAQDTSGLR 
      LHYTSDLRE NEGGILTTG IALSSLHFI PPYQREYKT 
      AGYCNTDCT KATFPKEGI HVVSVMLHS HLAGRKLKL 
      RHIRAGREL PPLAQDEHY DFNYQQSRA LSQDVIILP 
      GDGLVTECT YSTLNRNKP TLGGYSTKE EMCLAFVLH 
      YPRTQLAGC YSMSPIKYF FKNLGVNKF YGKNMEEIE 
      NIFLHGEAE TNPVSPSTS PNKPLFPYQ PGDEMSPEA 
      NRRAIIALQ NAKDYTIEG ETHEGQTMF DKLVIEDPE 
      EFRNKSFMD HLQDIPYNE SLITRKMEE YFYTGLHLT 
      FCRKRDDSL AIKESIVNF PNFTVYSDD QKVQCSFKL 
      KRSPSSSVS IKSRFVILL ILVFLQSGL
    • >TC034197 RB
      MSNRDICIT LSLILLIYP KRSFAGCNR PFAITIRPA 
      RGSMQVCGG VLLHARWAL TAAHCVYGI ETPSSIAVM 
      AKLTKFKKI IVERIFIYD LFDPKTLES DYSLLYVGN 
      NFPKMTFAM LCSKALFSY LLAKEQRNV QVVTWEENN 
      PSVKKGRLM VEKCRQVDI LAFAHCRFF FATTLNPEV 
      MFCTTSDKN GQTPCRGDS GSPILYGRV VLGSISFGD 
      GCKKLRRPS VYSRIDQGL GFINSSIKT LGYALSTTT 
      LPKEKHPVY YISAGNVPA RQTLLFRLP DWKEEGLPK 
      APAVNQTTI VPHAHSSNT NTGCARNVF DFCDCASVI 
      FECFDHGIE AKKAKMSRI ILLFVLVTT TTCDDLLDE 
      YVRHVDDVN KPNKVHQRR LRRDIADKW THSEVLDNV 
      GDVVLRWQP RHREILFRV EARTKGYVA IGFSPDGGK 
      ENADIVMGW VDDRTLRAY LLDCHGVPD SQGSVPIKD 
      EIDNYTLMS GSQNDTHTV IEFRRVLDT CDPNDYVLT 
      GDTVVVIWA YHNTDPHLG AEMIYHGDK RGAQSLHLL 
      GPPPVSKAS GGNIRNWDV TLRNFEIIS NMNTVYWCK 
      IFKAPTLQH KHHIVGYEP LIGSNHTTF VHHMILHEC 
      ELDGEDVHK WERFSKENG RLCYGSNMA PEWEKCLTP 
      LVAWAIGSK GENFPKHVG LPLAAKKNS FYMLEVHFD 
      NPAMKQAQD TSGLRLHYT SDLRENEGG ILTTGIALS 
      SLHFIPPYQ REYKTAGYC NTDCTKATF PKEGIHVVS 
      VMLHSHLAG RKLKLRHIR AGRELPPLA QDEHYDFNY 
      QQSRALSQD VIILPGDGL VTECTYSTL NRNKPTLGG 
      YSTKEEMCL AFVLHYPRT QLAGCYSMS PIKYFFKNL 
      GVNKFYGKN MEEIENIFL HGEAETNPV SPSTSPNKP 
      LFPYQPGDE MSPEANRRA IIALQNAKD YTIEGETHE 
      GQTMFDKLV IEDPEEFRN KSFMDHLQD IPYNESLIT 
      RKMEEYFYT GLHLTFCRK RDDSLAIKE SIVNFPNFT 
      VYSDDQKVQ CSFKLKRSP SSSVSIKSR FVILLILVF 
      LQSGL
    CDS sequence
    • >TC034197 RA
      atgtctcgc atcattctc ttgtttgtc cttgtgacg 
      accactacc tgtgatgac ctcctggac gagtacgtc 
      cggcacgtt gacgacgtc aataaacca aataaagtg 
      caccaaaga cgcctccgt cgagacatt gcagataaa 
      tggactcat tccgaggtc ttggataat gtcggtgat 
      gtggttttg aggtggcaa ccgcgacac cgggagatc 
      ctcttcaga gtggaagca aggacgaaa ggatacgtt 
      gccataggt ttcagtcca gatggaggc aaggaaaac 
      gcagatatt gtcatggga tgggtcgat gacaggact 
      ctaagggcc tacctcttg gactgccat ggggtgccc 
      gacagccaa ggaagtgtt cccatcaaa gatgaaatc 
      gataattat acgcttatg agcgggagc caaaacgat 
      actcatacc gttatcgaa tttagaagg gttttggac 
      acgtgtgac cccaacgac tacgtcctt acgggagat 
      acagttgtg gtaatttgg gcgtatcac aacaccgac 
      ccgcaccta ggggccgaa atgatttat cacggggat 
      aaaagaggg gcccagagt cttcactta ttgggaccc 
      ccgcctgtc tccaaggct tcggggggc aacatcagg 
      aattgggac gtaacgctt cgaaacttt gagataatc 
      agcaacatg aacacggtc tactggtgc aaaatcttc 
      aaagctcca accctccaa cacaagcac catatcgtg 
      gggtacgaa cccctaatt ggctctaac cacaccacc 
      ttcgtccac catatgatc cttcacgag tgcgaactc 
      gacggagaa gacgtccac aaatgggaa cgcttctcg 
      aaagaaaac ggacgtttg tgctacggc tctaacatg 
      gccccagag tgggaaaaa tgtcttacg ccacttgta 
      gcctgggct attgggtcc aaaggcgag aatttcccc 
      aaacatgtt ggacttccg ctagcagca aagaaaaat 
      tcgttttac atgttggaa gtccatttt gataatccg 
      gcaatgaag caagcccaa gacacgtca ggacttcgg 
      ttacattac accagtgat ttgagagaa aatgaagga 
      gggattctt acaacaggg attgctcta tctagctta 
      catttcatt ccgccgtat cagagagaa tataaaacg 
      gccggttat tgtaacact gattgcacc aaagcaacg 
      tttccgaaa gagggaatt catgtggtt tcggttatg 
      ttgcattca catctagct ggcaggaaa ctaaagttg 
      agacacatc cgagctgga agggaactc ccacctttg 
      gcacaagac gagcattac gatttcaat taccaacaa 
      tcacgggct ttatcccag gatgttatt atcctacca 
      ggagatggt ctcgtaacc gaatgcacg tactccaca 
      cttaatcgc aacaaaccg acacttggg ggctactcc 
      accaaggag gagatgtgt ctagcgttc gttttacac 
      tacccgagg acgcaactc gctggttgt tactccatg 
      tctcctata aaatatttt ttcaaaaat ttgggagtc 
      aataaattc tacggcaag aatatggaa gagattgaa 
      aatattttc ttgcacgga gaggctgaa actaatccg 
      gtttcaccg tcaacttcg cccaataag ccacttttt 
      ccgtaccag ccgggtgac gaaatgtcg ccggaagcc 
      aacagaagg gccattata gccctacag aatgccaaa 
      gattatacc attgaagga gagacgcat gagggacaa 
      accatgttt gataaacta gtgatagag gatccggag 
      gagtttagg aacaagtcg tttatggat catttgcaa 
      gatattccc tacaacgag tcgctgatc actaggaaa 
      atggaggag tacttttac acggggctt cacttgact 
      ttttgtagg aaacgtgat gattcactt gcgattaag 
      gaaagcatc gtgaatttt ccaaatttt acggtttac 
      tccgatgac caaaaagtt caatgcagt tttaaactg 
      aaacgttca ccctcatcg tcagtgtcg attaaatcg 
      cgatttgtt attttactc attttggtg tttctccag 
      tcaggtctt taa
    • >TC034197 RB
      atgtcaaat cgtgacatt tgcataaca ttatcattg 
      atcctcctt atctaccca aaacgaagc tttgctggt 
      tgcaaccgt ccgttcgcc atcaccatc agaccggca 
      agaggttca atgcaagtc tgtggtgga gttctcctt 
      cacgcaaga tgggctcta acggcagct cattgtgtt 
      tacggcatt gaaacaccg tcaagcatc gccgtgatg 
      gcaaaactg accaaattc aagaaaatc atcgttgaa 
      cgaatcttc atttacgat ttgttcgac cccaaaaca 
      ttagaaagc gattacagt ttgttgtac gttgggaat 
      aattttccc aaaatgact ttcgctatg ctttgtagc 
      aaagcactg ttttcgtat cttttggca aaggagcaa 
      cgaaacgta caagttgta acgtgggaa gagaataac 
      ccgtctgtt aaaaaagga cggttaatg gtggagaag 
      tgtcgtcag gtcgatatt cttgcgttc gcacattgt 
      agatttttt ttcgctacg actttgaac cccgaagtt 
      atgttttgt acaacgagt gataaaaat ggtcaaaca 
      ccttgtaga ggtgattct gggagtccc attctttac 
      gggagggtg gtcttaggg tcgatttca tttggcgat 
      ggttgcaag aaattacgg agaccttca gtctacagc 
      aggattgat caaggactt ggtttcatc aatagttct 
      attaaaaca cttggctat gctttgagc acaaccaca 
      ctccccaaa gagaaacac cctgtgtac tacatttca 
      gcaggaaat gttcctgca agacaaaca ctcctattt 
      cggttgcct gattggaag gaagagggt ctgcccaag 
      gcaccggca gtcaaccag actaccatc gtaccacat 
      gcacacagt tctaacaca aacacagga tgtgctcgg 
      aacgttttc gatttttgt gattgtgcg agtgtgatt 
      ttcgagtgt tttgaccat ggaatagaa gcgaaaaaa 
      gccaaaatg tctcgcatc attctcttg tttgtcctt 
      gtgacgacc actacctgt gatgacctc ctggacgag 
      tacgtccgg cacgttgac gacgtcaat aaaccaaat 
      aaagtgcac caaagacgc ctccgtcga gacattgca 
      gataaatgg actcattcc gaggtcttg gataatgtc 
      ggtgatgtg gttttgagg tggcaaccg cgacaccgg 
      gagatcctc ttcagagtg gaagcaagg acgaaagga 
      tacgttgcc ataggtttc agtccagat ggaggcaag 
      gaaaacgca gatattgtc atgggatgg gtcgatgac 
      aggactcta agggcctac ctcttggac tgccatggg 
      gtgcccgac agccaagga agtgttccc atcaaagat 
      gaaatcgat aattatacg cttatgagc gggagccaa 
      aacgatact cataccgtt atcgaattt agaagggtt 
      ttggacacg tgtgacccc aacgactac gtccttacg 
      ggagataca gttgtggta atttgggcg tatcacaac 
      accgacccg cacctaggg gccgaaatg atttatcac 
      ggggataaa agaggggcc cagagtctt cacttattg 
      ggacccccg cctgtctcc aaggcttcg gggggcaac 
      atcaggaat tgggacgta acgcttcga aactttgag 
      ataatcagc aacatgaac acggtctac tggtgcaaa 
      atcttcaaa gctccaacc ctccaacac aagcaccat 
      atcgtgggg tacgaaccc ctaattggc tctaaccac 
      accaccttc gtccaccat atgatcctt cacgagtgc 
      gaactcgac ggagaagac gtccacaaa tgggaacgc 
      ttctcgaaa gaaaacgga cgtttgtgc tacggctct 
      aacatggcc ccagagtgg gaaaaatgt cttacgcca 
      cttgtagcc tgggctatt gggtccaaa ggcgagaat 
      ttccccaaa catgttgga cttccgcta gcagcaaag 
      aaaaattcg ttttacatg ttggaagtc cattttgat 
      aatccggca atgaagcaa gcccaagac acgtcagga 
      cttcggtta cattacacc agtgatttg agagaaaat 
      gaaggaggg attcttaca acagggatt gctctatct 
      agcttacat ttcattccg ccgtatcag agagaatat 
      aaaacggcc ggttattgt aacactgat tgcaccaaa 
      gcaacgttt ccgaaagag ggaattcat gtggtttcg 
      gttatgttg cattcacat ctagctggc aggaaacta 
      aagttgaga cacatccga gctggaagg gaactccca 
      cctttggca caagacgag cattacgat ttcaattac 
      caacaatca cgggcttta tcccaggat gttattatc 
      ctaccagga gatggtctc gtaaccgaa tgcacgtac 
      tccacactt aatcgcaac aaaccgaca cttgggggc 
      tactccacc aaggaggag atgtgtcta gcgttcgtt 
      ttacactac ccgaggacg caactcgct ggttgttac 
      tccatgtct cctataaaa tattttttc aaaaatttg 
      ggagtcaat aaattctac ggcaagaat atggaagag 
      attgaaaat attttcttg cacggagag gctgaaact 
      aatccggtt tcaccgtca acttcgccc aataagcca 
      ctttttccg taccagccg ggtgacgaa atgtcgccg 
      gaagccaac agaagggcc attatagcc ctacagaat 
      gccaaagat tataccatt gaaggagag acgcatgag 
      ggacaaacc atgtttgat aaactagtg atagaggat 
      ccggaggag tttaggaac aagtcgttt atggatcat 
      ttgcaagat attccctac aacgagtcg ctgatcact 
      aggaaaatg gaggagtac ttttacacg gggcttcac 
      ttgactttt tgtaggaaa cgtgatgat tcacttgcg 
      attaaggaa agcatcgtg aattttcca aattttacg 
      gtttactcc gatgaccaa aaagttcaa tgcagtttt 
      aaactgaaa cgttcaccc tcatcgtca gtgtcgatt 
      aaatcgcga tttgttatt ttactcatt ttggtgttt 
      ctccagtca ggtctttaa
    mRNA sequence
    • >TC034197 RA
      ttctgtaca aaaaggatg gcggtcagt ggcattacg 
      agcacaata aaactcccg gtgtaatat tggcgaagg 
      tcccatagg tagcggttg cctgattgg aaggaagag 
      ggtctgccc aaggcaccg gcagtcaac cagactacc 
      atcgtacca catgcacac agttctaac acaaacaca 
      ggatgtgct cggaacgtt ttcgatttt tgtgattgt 
      gcgagtgtg attttcgag tgttttgac catggaata 
      gaagcgaaa aaaggtaaa gaatgtcgt tcacacgga 
      cccattgtt attccaaca acaaactct ggcttcgct 
      tttaaagtg gcttctgga ctgctaatt aatttggtc 
      ttaatatta aaccagaac gattagtca ctcagacta 
      tctaaaaat aaataatgt gatcaaaaa tgtttttac 
      gttcgctcc ctttttttt tgataattt tattttcat 
      ttgacaaat tgacaaata attgtaaga tctttgatt 
      tttgttagt actgtatga ccatccatc gttttcttt 
      gttgtaaat agctgcgtt taaaacaat gctgttcca 
      catctagaa tttatgaaa acgcttagc gaaggctta 
      aatagctgc gagcttgag aatctcctc ggaattttg 
      ctgcaatgt cgcgagctt ccaactttt ttcaatgta 
      cctagctac attaaaata gttattttt ttgtaatga 
      cacgccaag acatgagtg tatttgtac gacacacct 
      gcagagtcc cctcaggca taccgatgg tatttttaa 
      ccttttcac catcaatat taaaactcg tagtttgtt 
      gatagattt tcagttgca atttcctga cggaattca 
      atttaaatc agtttactt tcagtaatc ccatcaatc 
      gcatttccg tcgaaattt attgcaagt cgaaaaaat 
      ttaattagt attttaaga gtctggaaa aactgagat 
      taataaaca acgtttgcc ttttcaaac gcagctgca 
      cgattatca aggggttaa cattgctag cagtgtatt 
      gtataataa ttttctagt tgtttgtca ttgtgttgt 
      agttacatg cctccaaac acaaacgac attaatatt 
      tattaggtg ggagagcct gccagcggg ggtgataaa 
      ttcattgag agacgaaga agttttata ctccaataa 
      ccgtaattc gaaatagag ttggacata tacatctat 
      ctctaaaac agattttta atgcaattt cttctgcga 
      gattattat atctctata aacaatgtc taagccgca 
      caatttcct accaactcg tctaaaaat gtaaaattt 
      tagccaaaa tgtctcgca tcattctct tgtttgtcc 
      ttgtgacga ccactacct gtgatgacc tcctggacg 
      agtacgtcc ggcacgttg acgacgtca ataaaccaa 
      ataaagtgc accaaagac gcctccgtc gagacattg 
      cagataaat ggactcatt ccgaggtct tggataatg 
      tcggtgatg tggttttga ggtggcaac cgcgacacc 
      gggagatcc tcttcagag tggaagcaa ggacgaaag 
      gatacgttg ccataggtt tcagtccag atggaggca 
      aggaaaacg cagatattg tcatgggat gggtcgatg 
      acaggactc taagggcct acctcttgg actgccatg 
      gggtgcccg acagccaag gaagtgttc ccatcaaag 
      atgaaatcg ataattata cgcttatga gcgggagcc 
      aaaacgata ctcataccg ttatcgaat ttagaaggg 
      ttttggaca cgtgtgacc ccaacgact acgtcctta 
      cgggagata cagttgtgg taatttggg cgtatcaca 
      acaccgacc cgcacctag gggccgaaa tgatttatc 
      acggggata aaagagggg cccagagtc ttcacttat 
      tgggacccc cgcctgtct ccaaggctt cggggggca 
      acatcagga attgggacg taacgcttc gaaactttg 
      agataatca gcaacatga acacggtct actggtgca 
      aaatcttca aagctccaa ccctccaac acaagcacc 
      atatcgtgg ggtacgaac ccctaattg gctctaacc 
      acaccacct tcgtccacc atatgatcc ttcacgagt 
      gcgaactcg acggagaag acgtccaca aatgggaac 
      gcttctcga aagaaaacg gacgtttgt gctacggct 
      ctaacatgg ccccagagt gggaaaaat gtcttacgc 
      cacttgtag cctgggcta ttgggtcca aaggcgaga 
      atttcccca aacatgttg gacttccgc tagcagcaa 
      agaaaaatt cgttttaca tgttggaag tccattttg 
      ataatccgg caatgaagc aagcccaag acacgtcag 
      gacttcggt tacattaca ccagtgatt tgagagaaa 
      atgaaggag ggattctta caacaggga ttgctctat 
      ctagcttac atttcattc cgccgtatc agagagaat 
      ataaaacgg ccggttatt gtaacactg attgcacca 
      aagcaacgt ttccgaaag agggaattc atgtggttt 
      cggttatgt tgcattcac atctagctg gcaggaaac 
      taaagttga gacacatcc gagctggaa gggaactcc 
      cacctttgg cacaagacg agcattacg atttcaatt 
      accaacaat cacgggctt tatcccagg atgttatta 
      tcctaccag gagatggtc tcgtaaccg aatgcacgt 
      actccacac ttaatcgca acaaaccga cacttgggg 
      gctactcca ccaaggagg agatgtgtc tagcgttcg 
      ttttacact acccgagga cgcaactcg ctggttgtt 
      actccatgt ctcctataa aatattttt tcaaaaatt 
      tgggagtca ataaattct acggcaaga atatggaag 
      agattgaaa atattttct tgcacggag aggctgaaa 
      ctaatccgg tttcaccgt caacttcgc ccaataagc 
      cactttttc cgtaccagc cgggtgacg aaatgtcgc 
      cggaagcca acagaaggg ccattatag ccctacaga 
      atgccaaag attatacca ttgaaggag agacgcatg 
      agggacaaa ccatgtttg ataaactag tgatagagg 
      atccggagg agtttagga acaagtcgt ttatggatc 
      atttgcaag atattccct acaacgagt cgctgatca 
      ctaggaaaa tggaggagt acttttaca cggggcttc 
      acttgactt tttgtagga aacgtgatg attcacttg 
      cgattaagg aaagcatcg tgaattttc caaatttta 
      cggtttact ccgatgacc aaaaagttc aatgcagtt 
      ttaaactga aacgttcac cctcatcgt cagtgtcga 
      ttaaatcgc gatttgtta ttttactca ttttggtgt 
      ttctccagt caggtcttt aaagcaacc aatgtaaat 
      aatggtctt gtacatatc attcaccaa ataattatt 
      tataaatag gtttaagtt tcaatgctg tgagttgat 
      taagtcatc cggatacat gtattcgac tttttgtac 
      ttaataaac gttctagtt ttac
    • >TC034197 RB
      agaggattc cgtaatcta gaaataatt ctgcatctc 
      gaagtttat cttgatgtc aaatcgtga catttgcat 
      aacattatc attgatcct ccttatcta cccaaaacg 
      aagctttgc tggttgcaa ccgtccgtt cgccatcac 
      catcagacc ggcaagagg ttcaatgca agtctgtgg 
      tggagttct ccttcacgc aagatgggc tctaacggc 
      agctcattg tgtttacgg cattgaaac accgtcaag 
      catcgccgt gatggcaaa actgaccaa attcaagaa 
      aatcatcgt tgaacgaat cttcattta cgatttgtt 
      cgaccccaa aacattaga aagcgatta cagtttgtt 
      gtacgttgg gaataattt tcccaaaat gactttcgc 
      tatgctttg tagcaaagc actgttttc gtatctttt 
      ggcaaagga gcaacgaaa cgtacaagt tgtaacgtg 
      ggaagagaa taacccgtc tgttaaaaa aggacggtt 
      aatggtgga gaagtgtcg tcaggtcga tattcttgc 
      gttcgcaca ttgtagatt ttttttcgc tacgacttt 
      gaaccccga agttatgtt ttgtacaac gagtgataa 
      aaatggtca aacaccttg tagaggtga ttctgggag 
      tcccattct ttacgggag ggtggtctt agggtcgat 
      ttcatttgg cgatggttg caagaaatt acggagacc 
      ttcagtcta cagcaggat tgatcaagg acttggttt 
      catcaatag ttctattaa aacacttgg ctatgcttt 
      gagcacaac cacactccc caaagagaa acaccctgt 
      gtactacat ttcagcagg aaatgttcc tgcaagaca 
      aacactcct atttcggtt gcctgattg gaaggaaga 
      gggtctgcc caaggcacc ggcagtcaa ccagactac 
      catcgtacc acatgcaca cagttctaa cacaaacac 
      aggatgtgc tcggaacgt tttcgattt ttgtgattg 
      tgcgagtgt gattttcga gtgttttga ccatggaat 
      agaagcgaa aaaagccaa aatgtctcg catcattct 
      cttgtttgt ccttgtgac gaccactac ctgtgatga 
      cctcctgga cgagtacgt ccggcacgt tgacgacgt 
      caataaacc aaataaagt gcaccaaag acgcctccg 
      tcgagacat tgcagataa atggactca ttccgaggt 
      cttggataa tgtcggtga tgtggtttt gaggtggca 
      accgcgaca ccgggagat cctcttcag agtggaagc 
      aaggacgaa aggatacgt tgccatagg tttcagtcc 
      agatggagg caaggaaaa cgcagatat tgtcatggg 
      atgggtcga tgacaggac tctaagggc ctacctctt 
      ggactgcca tggggtgcc cgacagcca aggaagtgt 
      tcccatcaa agatgaaat cgataatta tacgcttat 
      gagcgggag ccaaaacga tactcatac cgttatcga 
      atttagaag ggttttgga cacgtgtga ccccaacga 
      ctacgtcct tacgggaga tacagttgt ggtaatttg 
      ggcgtatca caacaccga cccgcacct aggggccga 
      aatgattta tcacgggga taaaagagg ggcccagag 
      tcttcactt attgggacc cccgcctgt ctccaaggc 
      ttcgggggg caacatcag gaattggga cgtaacgct 
      tcgaaactt tgagataat cagcaacat gaacacggt 
      ctactggtg caaaatctt caaagctcc aaccctcca 
      acacaagca ccatatcgt ggggtacga acccctaat 
      tggctctaa ccacaccac cttcgtcca ccatatgat 
      ccttcacga gtgcgaact cgacggaga agacgtcca 
      caaatggga acgcttctc gaaagaaaa cggacgttt 
      gtgctacgg ctctaacat ggccccaga gtgggaaaa 
      atgtcttac gccacttgt agcctgggc tattgggtc 
      caaaggcga gaatttccc caaacatgt tggacttcc 
      gctagcagc aaagaaaaa ttcgtttta catgttgga 
      agtccattt tgataatcc ggcaatgaa gcaagccca 
      agacacgtc aggacttcg gttacatta caccagtga 
      tttgagaga aaatgaagg agggattct tacaacagg 
      gattgctct atctagctt acatttcat tccgccgta 
      tcagagaga atataaaac ggccggtta ttgtaacac 
      tgattgcac caaagcaac gtttccgaa agagggaat 
      tcatgtggt ttcggttat gttgcattc acatctagc 
      tggcaggaa actaaagtt gagacacat ccgagctgg 
      aagggaact cccaccttt ggcacaaga cgagcatta 
      cgatttcaa ttaccaaca atcacgggc tttatccca 
      ggatgttat tatcctacc aggagatgg tctcgtaac 
      cgaatgcac gtactccac acttaatcg caacaaacc 
      gacacttgg gggctactc caccaagga ggagatgtg 
      tctagcgtt cgttttaca ctacccgag gacgcaact 
      cgctggttg ttactccat gtctcctat aaaatattt 
      tttcaaaaa tttgggagt caataaatt ctacggcaa 
      gaatatgga agagattga aaatatttt cttgcacgg 
      agaggctga aactaatcc ggtttcacc gtcaacttc 
      gcccaataa gccactttt tccgtacca gccgggtga 
      cgaaatgtc gccggaagc caacagaag ggccattat 
      agccctaca gaatgccaa agattatac cattgaagg 
      agagacgca tgagggaca aaccatgtt tgataaact 
      agtgataga ggatccgga ggagtttag gaacaagtc 
      gtttatgga tcatttgca agatattcc ctacaacga 
      gtcgctgat cactaggaa aatggagga gtactttta 
      cacggggct tcacttgac tttttgtag gaaacgtga 
      tgattcact tgcgattaa ggaaagcat cgtgaattt 
      tccaaattt tacggttta ctccgatga ccaaaaagt 
      tcaatgcag ttttaaact gaaacgttc accctcatc 
      gtcagtgtc gattaaatc gcgatttgt tattttact 
      cattttggt gtttctcca gtcaggtct ttaaagcaa 
      ccaatgtaa ataatggtc ttgtacata tcattcacc 
      aaataatta tttataaat aggtttaag tttcaatgc 
      tgtgagttg attaagtca tccggatac atgtattcg 
      actttttgt acttaataa acgttctag ttttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY