Status
Please wait ...
Gene information
General information
TC034199
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005323,TC005324
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14484865..14490962
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034199
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03936

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number >150% of expected -
    Comment: No phenotype found!!!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034199 RA
      MCEKSDETR CNLTNILMQ LRRHLSVNH NVAALSQRF 
      GALNSNLER VNFVEELIK SAEITIELQ KDVKNDEIA 
      TKYREKGNE MYKKRKLKE AWEFYTKSV CFAEENSTN 
      LGLAYANRS AVLFELNLH KQCLKDIKR ALNHNYPEH 
      LKSKLFVRE KNSKNTAIE ETCPHEAIP IVTNNNPNI 
      QSASACIAV REEENWGRF VVATRDIKV GEVLAVEKP 
      LVTLVVNEL SNHCHECVS LCYNLIPCK TCTQAMYCS 
      ESCRDYAFD MYHKYECSI LATLRFLQF DKLKLFALK 
      ISLQYNDLG ETSDLLYRS DRYREIHNL VTNTTKRSV 
      PDLFERATA AALIYDLVK THTNFFSAF NQNNFKEIL 
      LLHMQTGPS NFHEIVELV PNSRGIYEP EEIASGAFA 
      FLSLLNHSC CPNVARFSY GSTLVLRAI QNIQEGEQC 
      FDNYGYHFA LMDKSERKK HLQSQYYFN CVCQACEKN 
      WPLFDSLPS FNSHEIEDS VFLKLSSGD VETAVTVAG 
      DLCTKLGEL QKLQPCKDF CQLQEIVKQ YIVSVVPPK 
      WFGGGTAVP QCAVWSTAP AQPVAKMQL IYFFTLFSF 
      AVSEDLKLT ILHNNDLHS RFEETSRNS GTCKDKKEC 
      VGGFARTAH EIRRFRTES GDNPVLFLN AGDTYVGTA 
      WFAVHKWKI CVEFLNLLK PDVMSLGNH EFDFGVSSL 
      APFVKNAQF PIVAANLDF TKEPSLSEI KKSVVIDIS 
      GRKVGIVGH LTPDTRIMS QPGAVEFLD VVESVKNET 
      QVLDAAGVK IIIVLGHSG YDMDLKIAA DVPLADVVI 
      GGHTNTFLW NGAQPDLEK PEDMYPKVV VQKGGKKVP 
      VVQAYAYAK YLGVLNVTF DGQGDLVGF QGQPIFLDN 
      GIVQDQDVL DLLEKYRPA VDEINKEVV GKSAVVLDA 
      SANKCRVVE CNIGNLIAD SFVYYMAGQ YAGPGWTDT 
      PIGLINGGS IRTIIDPSV HGGNITRGE LMGTLPFDN 
      QVVSFKLTG SQILKTLEI GARSNGETS KGEFLQFSG 
      LHVVYNKKK PAFERVVSV KVRCGNCSV PLYEPLDPK 
      KTYGVVTAS FLTNGGDGH EVLANAPNK KVQDLGDVD 
      TVVWYLKRQ TEVYPEEQG RIKFVESEG GNTPSGATR 
      TFGVLALVL TLVTKVVIV
    CDS sequence
    • >TC034199 RA
      atgtgcgaa aaatccgat gaaacccgt tgtaacttg 
      accaacatc ctcatgcaa ttgcggcgg cacctatcg 
      gtaaaccat aacgttgcg gccttgtca caacgtttc 
      ggcgctcta aattcgaat ttggaacgt gttaatttt 
      gtcgaagaa ttgataaaa tcagcggaa atcactatc 
      gagttgcaa aaagacgtg aaaaatgat gaaattgcg 
      acgaaatat cgcgagaaa ggtaacgaa atgtataaa 
      aaaagaaaa ctgaaagaa gcgtgggaa ttttacacg 
      aaaagtgtg tgttttgcg gaggaaaat agcacgaat 
      ttgggctta gcgtacgcg aaccgatca gcagttttg 
      tttgaattg aatttgcac aagcaatgt ttaaaggat 
      attaaacgc gccttgaat cacaattat cccgaacat 
      ctcaaatcg aaactattt gtaagggaa aaaaattcg 
      aagaatacc gcaattgag gagacttgt ccacatgaa 
      gcgattcca attgtcaca aacaacaat ccgaatatt 
      caaagcgct agcgcttgt attgccgta cgagaagag 
      gaaaattgg ggacgtttt gtggtggct actagagac 
      attaaagtc ggggaagta ctcgctgta gaaaaacca 
      ttggttacc cttgttgtc aacgaattg tcaaatcat 
      tgccacgaa tgcgtttcc ttgtgttac aatttaatt 
      ccttgcaaa acttgcacc caggctatg tattgtagc 
      gaaagttgt cgcgattat gcttttgat atgtatcac 
      aagtacgaa tgttcaata cttgcaacg ctgcgattt 
      ttacaattc gataaactc aaattattc gctttaaaa 
      atctcactt cagtacaac gacttgggg gaaacctct 
      gatttgttg taccgttcg gatcgctac agagagatt 
      cacaattta gtgacaaac acgacaaaa cggtccgtt 
      ccagattta ttcgaaagg gccacagct gcggctttg 
      atttatgac ttggtcaag actcatacg aattttttt 
      agcgccttc aatcaaaat aattttaaa gaaatttta 
      ctgttgcat atgcaaacc ggtccttct aattttcac 
      gaaatcgtc gaactcgta cctaattcg agaggtatt 
      tacgaacct gaagaaatc gcaagcggt gcatttgca 
      tttttgagt cttttgaat cactcttgt tgccccaat 
      gtggcccga ttctcctac ggttctact ctagtgctc 
      agagcgatc caaaatatt caagaaggg gagcaatgt 
      tttgataat tacgggtat cacttcgct ttgatggat 
      aaaagcgag cgcaaaaaa caccttcaa agtcagtat 
      tacttcaac tgcgtttgt caggcttgt gagaaaaat 
      tggccgttg tttgactct ttgccctct tttaacagt 
      catgaaatt gaagactct gtcttttta aaattatca 
      tcgggtgat gttgagact gctgtgact gttgcgggg 
      gatttatgc accaaattg ggggaatta cagaagttg 
      caaccgtgc aaagacttt tgccaactg caggaaatc 
      gtaaaacaa tacattgtc tctgttgtc cctcccaaa 
      tggtttgga ggcggaaca gcagttccc caatgtgca 
      gtctggtca acggcgcca gcacaacca gtcgccaaa 
      atgcagctc atttatttc ttcacactc ttctccttt 
      gccgtatcc gaagacttg aagttgact attttgcac 
      aacaacgac ctgcacagc cgttttgag gagacttca 
      cgcaattcc gggacgtgt aaagacaag aaagagtgc 
      gttggagga tttgcaagg acggcacat gagatccgg 
      aggttccgg acggagtcc ggagataat ccggtgttg 
      ttcctaaat gccggggac acgtatgtg gggacggca 
      tggttcgca gtgcataag tggaagatt tgtgtcgaa 
      tttcttaat ttgcttaaa ccggatgtc atgtcttta 
      ggtaaccac gaatttgat tttggcgtc tcatccctt 
      gcccctttc gtcaaaaac gcccaattc cccatcgtt 
      gctgctaac ctcgacttc accaaagaa ccatctctc 
      agcgaaata aaaaaatcg gtggtaatc gacatttcc 
      ggtcgaaaa gtcggtatc gtcggtcac ctcaccccc 
      gatactcgc atcatgtct caacctgga gctgttgaa 
      tttttggac gtggtcgaa tccgtcaaa aacgaaacc 
      caagttctt gacgcagct ggcgtcaaa ataatcatc 
      gttttgggg cactcaggc tacgacatg gacttgaaa 
      atcgcagcg gacgtgcct ttggccgac gttgtcatt 
      gggggacac actaacaca tttttgtgg aatggggca 
      cagcctgat ttagagaaa cccgaagac atgtatccc 
      aaagtcgtg gtccaaaaa gggggcaag aaggtgcca 
      gtggtccaa gcgtacgct tatgcgaaa tatttgggg 
      gttttgaac gttacgttt gatgggcaa ggcgatttg 
      gtgggtttt cagggccaa ccgattttt ctcgataat 
      gggatagtg caagatcag gacgttttg gatttgctg 
      gagaaatac agaccggct gttgatgaa ataaataag 
      gaagttgtg gggaaatcg gctgtggtt ctggacgct 
      tcagcgaat aaatgtcga gtggttgaa tgtaatatt 
      ggaaatttg attgctgat tcgtttgtt tactacatg 
      gcgggtcaa tatgccggg cctggatgg accgataca 
      cccattggg ttgattaac ggtggtagt atacgtacc 
      atcattgac ccatcggtc catggcggt aacataaca 
      cgcggcgaa ctcatgggc actttacct ttcgataac 
      caagtcgtt tcattcaaa ctgaccggt tctcaaatc 
      cttaaaacg cttgaaatc ggagctagg agtaatgga 
      gagacgtcc aaaggcgag tttttgcaa ttttcgggt 
      ttgcatgtc gtttataat aagaaaaaa ccggcgttt 
      gaacgcgta gtatcggtt aaagtccgt tgtggaaat 
      tgtagtgtt cccctttat gaaccttta gatcctaag 
      aaaacttac ggagttgtt acggctagt tttttgact 
      aatgggggt gatggacat gaagttttg gcaaatgcg 
      cccaataaa aaagtacaa gatttgggc gatgttgat 
      accgttgtg tggtatttg aagaggcag actgaggta 
      tatccggag gaacaaggg aggattaaa tttgttgaa 
      agtgagggt ggaaacaca ccgtcgggg gccactagg 
      acttttggt gtactagct ttggttcta actttagtt 
      acgaaggtt gtaatagtc tga
    mRNA sequence
    • >TC034199 RA
      tgagtgatc tattttagt ttcgtgccc cgtgtttga 
      attaatatt ccctcaaaa ctccaccca acaacacaa 
      ttatttatt attaagaat tcataaata aaatatgtg 
      cgaaaaatc cgatgaaac ccgttgtaa cttgaccaa 
      catcctcat gcaattgcg gcggcacct atcggtaaa 
      ccataacgt tgcggcctt gtcacaacg tttcggcgc 
      tctaaattc gaatttgga acgtgttaa ttttgtcga 
      agaattgat aaaatcagc ggaaatcac tatcgagtt 
      gcaaaaaga cgtgaaaaa tgatgaaat tgcgacgaa 
      atatcgcga gaaaggtaa cgaaatgta taaaaaaag 
      aaaactgaa agaagcgtg ggaatttta cacgaaaag 
      tgtgtgttt tgcggagga aaatagcac gaatttggg 
      cttagcgta cgcgaaccg atcagcagt tttgtttga 
      attgaattt gcacaagca atgtttaaa ggatattaa 
      acgcgcctt gaatcacaa ttatcccga acatctcaa 
      atcgaaact atttgtaag ggaaaaaaa ttcgaagaa 
      taccgcaat tgaggagac ttgtccaca tgaagcgat 
      tccaattgt cacaaacaa caatccgaa tattcaaag 
      cgctagcgc ttgtattgc cgtacgaga agaggaaaa 
      ttggggacg ttttgtggt ggctactag agacattaa 
      agtcgggga agtactcgc tgtagaaaa accattggt 
      tacccttgt tgtcaacga attgtcaaa tcattgcca 
      cgaatgcgt ttccttgtg ttacaattt aattccttg 
      caaaacttg cacccaggc tatgtattg tagcgaaag 
      ttgtcgcga ttatgcttt tgatatgta tcacaagta 
      cgaatgttc aatacttgc aacgctgcg atttttaca 
      attcgataa actcaaatt attcgcttt aaaaatctc 
      acttcagta caacgactt gggggaaac ctctgattt 
      gttgtaccg ttcggatcg ctacagaga gattcacaa 
      tttagtgac aaacacgac aaaacggtc cgttccaga 
      tttattcga aagggccac agctgcggc tttgattta 
      tgacttggt caagactca tacgaattt ttttagcgc 
      cttcaatca aaataattt taaagaaat tttactgtt 
      gcatatgca aaccggtcc ttctaattt tcacgaaat 
      cgtcgaact cgtacctaa ttcgagagg tatttacga 
      acctgaaga aatcgcaag cggtgcatt tgcattttt 
      gagtctttt gaatcactc ttgttgccc caatgtggc 
      ccgattctc ctacggttc tactctagt gctcagagc 
      gatccaaaa tattcaaga aggggagca atgttttga 
      taattacgg gtatcactt cgctttgat ggataaaag 
      cgagcgcaa aaaacacct tcaaagtca gtattactt 
      caactgcgt ttgtcaggc ttgtgagaa aaattggcc 
      gttgtttga ctctttgcc ctcttttaa cagtcatga 
      aattgaaga ctctgtctt tttaaaatt atcatcggg 
      tgatgttga gactgctgt gactgttgc gggggattt 
      atgcaccaa attggggga attacagaa gttgcaacc 
      gtgcaaaga cttttgcca actgcagga aatcgtaaa 
      acaatacat tgtctctgt tgtccctcc caaatggtt 
      tggaggcgg aacagcagt tccccaatg tgcagtctg 
      gtcaacggc gccagcaca accagtcgc caaaatgca 
      gctcattta tttcttcac actcttctc ctttgccgt 
      atccgaaga cttgaagtt gactatttt gcacaacaa 
      cgacctgca cagccgttt tgaggagac ttcacgcaa 
      ttccgggac gtgtaaaga caagaaaga gtgcgttgg 
      aggatttgc aaggacggc acatgagat ccggaggtt 
      ccggacgga gtccggaga taatccggt gttgttcct 
      aaatgccgg ggacacgta tgtggggac ggcatggtt 
      cgcagtgca taagtggaa gatttgtgt cgaatttct 
      taatttgct taaaccgga tgtcatgtc tttaggtaa 
      ccacgaatt tgattttgg cgtctcatc ccttgcccc 
      tttcgtcaa aaacgccca attccccat cgttgctgc 
      taacctcga cttcaccaa agaaccatc tctcagcga 
      aataaaaaa atcggtggt aatcgacat ttccggtcg 
      aaaagtcgg tatcgtcgg tcacctcac ccccgatac 
      tcgcatcat gtctcaacc tggagctgt tgaattttt 
      ggacgtggt cgaatccgt caaaaacga aacccaagt 
      tcttgacgc agctggcgt caaaataat catcgtttt 
      ggggcactc aggctacga catggactt gaaaatcgc 
      agcggacgt gcctttggc cgacgttgt cattggggg 
      acacactaa cacattttt gtggaatgg ggcacagcc 
      tgatttaga gaaacccga agacatgta tcccaaagt 
      cgtggtcca aaaaggggg caagaaggt gccagtggt 
      ccaagcgta cgcttatgc gaaatattt gggggtttt 
      gaacgttac gtttgatgg gcaaggcga tttggtggg 
      ttttcaggg ccaaccgat ttttctcga taatgggat 
      agtgcaaga tcaggacgt tttggattt gctggagaa 
      atacagacc ggctgttga tgaaataaa taaggaagt 
      tgtggggaa atcggctgt ggttctgga cgcttcagc 
      gaataaatg tcgagtggt tgaatgtaa tattggaaa 
      tttgattgc tgattcgtt tgtttacta catggcggg 
      tcaatatgc cgggcctgg atggaccga tacacccat 
      tgggttgat taacggtgg tagtatacg taccatcat 
      tgacccatc ggtccatgg cggtaacat aacacgcgg 
      cgaactcat gggcacttt acctttcga taaccaagt 
      cgtttcatt caaactgac cggttctca aatccttaa 
      aacgcttga aatcggagc taggagtaa tggagagac 
      gtccaaagg cgagttttt gcaattttc gggtttgca 
      tgtcgttta taataagaa aaaaccggc gtttgaacg 
      cgtagtatc ggttaaagt ccgttgtgg aaattgtag 
      tgttcccct ttatgaacc tttagatcc taagaaaac 
      ttacggagt tgttacggc tagtttttt gactaatgg 
      gggtgatgg acatgaagt tttggcaaa tgcgcccaa 
      taaaaaagt acaagattt gggcgatgt tgataccgt 
      tgtgtggta tttgaagag gcagactga ggtatatcc 
      ggaggaaca agggaggat taaatttgt tgaaagtga 
      gggtggaaa cacaccgtc gggggccac taggacttt 
      tggtgtact agctttggt tctaacttt agttacgaa 
      ggttgtaat agtctgaat aaattctat tacactagt 
      ctcagattt tgttgctat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY