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Gene information
General information
TC005292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1711: 11319..15136
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005292
      iBeetle screen iB_03934
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005292 RA
        MSDTVSSLA KRSINLIRE YISKIKTLD EINSFSKYI 
        NRSVKIVSK ALINKIHET NIHENPQSL EASLAHLKY 
        YRLKLKKIK KDLLHNKSS KVGQGLSNV PKKTFGQKV 
        LWEDLNSCF KGRIRSGFI SNIHIKDPA IFLTRAYKS 
        FVLKIKKEL AKSMLKVNV VFSGNFIQP STLEIDLKT 
        FATRNNHID STTNIREWY HENVVSVIL NKLESFAEK 
        DSGWALYEI LGLKVNINQ YDPIKFGNN SSFVEVPNF 
        IKKKHCIIN VQNNDQFCF LWSVVSALY PVKNNPHRT 
        SSYPNFENV LRYDNISFP ITIRDIEKF EIMNNLSIN 
        LYCIEEKNK IFPARLSTY NKNNDKNIP TINLLMLPT 
        NNMDEEETH HNKKLYYHF VWIKHLSRL LSHQLSTHE 
        HKTFICERC LNYFTTEDI LTKHKIHCQ KLNQYSVSL 
        PRDTSLKFK NHLYKKKVP FVIYADLES ILVKCSQID 
        NNIHSKTIP HQKHIACSI AYYLKCSFD DSLSKFQLY 
        RNQDCIQWF VKELKDIAE QCDKIFKNK LPMEKLSYD 
        QERSYKESN VCHICNKTI ESDQIKVRD HNHLTGKFR 
        GPAHQRCNL NYQDSYTIP VVFHNLSGY DAHFIIKEL 
        STAFAGLTF DAMLKSTGI CLELLSDID MIMFIERGI 
        RGGVSQCSN RYAKANNHY MKDGYNSNE ELSYLMYFD 
        VNNLYGAAM SQYLPYGNF RFLEDFNIT EILNTSDTS 
        NDGYIIECD LEYPTPLHD LHSDLPLAP QHLKPPNSK 
        SKLEKLLLT LFPKINYIV HYRNLKMYL RLGLKITKV 
        HRVLTFNQS PWLKEYIDL NTRLRQQSQ NEFEKDFFK 
        LMINAIYGK CMENVRKHK DIRIVTRWD GRYGMRDLI 
        SKPNFHSSV IFEEDMSIV EMNKLNVVL NKPIYVGFC 
        ILDISKIFI YEFHYDYIK NKFGNNAKL LYTDTDSLI 
        YHFKTEDIY KNIKEDIHR FDTSDYKLD NKFNIELKN 
        KKCPGLMKD ENHGKIMLE FVGLRSKMY FYTVDSDTE 
        DEEHDYDEV GPAIKRKKF VKKSKGCTK SSIKHITFN 
        DYKKCLFDL DIFRSTQRL IRSKKHSVY SIKQEKVVL 
        SPYDDKRIL LRNTTDTRP WGYAINLT
      CDS sequence
      • >TC005292 RA
        atgtctgac acagtgagc tctttagct aaacgttct 
        ataaattta attcgtgaa tacattagt aaaattaaa 
        actttagat gaaattaat tcgttttcg aaatatatt 
        aataggagt gttaaaata gttagcaaa gctttgatc 
        aataaaatt catgaaaca aacattcat gaaaatcca 
        caatctttg gaagccagt cttgctcat ttgaaatac 
        tatcgactc aaattaaaa aaaattaaa aaagactta 
        ctccacaat aaaagcagt aaagttggt cagggatta 
        tcaaatgta ccgaaaaaa acatttggt caaaaggtt 
        ttatgggag gatttaaat tcttgtttt aaaggaaga 
        attcgttca ggttttatt tcaaatatt catataaaa 
        gatccagca atattttta acacgagca tataaaagt 
        tttgtatta aaaattaaa aaagaatta gcaaagtca 
        atgcttaaa gtaaatgta gtgttttca ggtaacttt 
        atacaacca tctacgttg gaaatagat ttaaaaaca 
        tttgcaact agaaataat catatagat tcaacaact 
        aatattaga gaatggtat catgaaaat gtagttagt 
        gtaatatta aataaatta gaatcattt gctgaaaaa 
        gatagtggt tgggcgtta tatgaaatt ttgggatta 
        aaggtaaat attaatcaa tatgatcca ataaaattt 
        ggaaataat tcaagtttt gtggaagta ccgaacttt 
        attaaaaaa aaacattgt ataataaat gtacagaat 
        aatgatcaa ttttgtttt ctttggtca gttgtgtct 
        gcgttatat cctgtaaaa aataatccc cacagaaca 
        tcatcatat ccaaatttt gaaaacgtt ttaaggtat 
        gataatata agttttcca ataacaatt agagatatt 
        gaaaaattt gaaataatg aacaattta tcaattaat 
        ttatattgt attgaagaa aaaaataaa atatttccg 
        gcacgttta agcacttat aataaaaat aatgataag 
        aatattcct acaataaat ttactcatg ctaccaaca 
        aataacatg gatgaagaa gaaacgcat cataataaa 
        aaattatat taccatttt gtatggatt aaacattta 
        tctcgcctt ttatcacat cagttgtct actcatgaa 
        cataaaaca tttatttgt gaacgatgt ttaaattat 
        tttacaacc gaagacatt ctaaccaaa cataaaatt 
        cactgtcaa aagcttaat cagtattca gtgtcatta 
        ccacgcgat acatctttg aaatttaaa aatcatctt 
        tataagaaa aaagtgcca tttgtaatt tatgctgat 
        ctcgaaagt attttggta aaatgttct caaattgat 
        aataatatt cacagtaaa actatccct caccaaaaa 
        catattgct tgtagtata gcttattac ttaaagtgt 
        tcatttgat gactcgctt tcaaaattt caattatat 
        cgtaatcaa gattgtatt cagtggttt gttaaggaa 
        ctaaaagac attgccgaa caatgtgat aaaatattt 
        aaaaataaa ttacccatg gaaaaactt agctacgat 
        caagaacgt tcatataaa gagtccaat gtttgtcac 
        atttgtaat aaaactatt gaatcagat caaattaaa 
        gttcgagat cataatcat ttaacggga aaatttaga 
        ggaccggct catcaacga tgtaattta aattatcaa 
        gattcatat accattcct gttgtgttc cataatttg 
        agtggatat gatgctcat tttattatt aaagaactg 
        tcaacagct tttgccgga ttaactttc gatgcaatg 
        ctgaaatct actggaata tgtttagag ctattaagt 
        gatatagat atgataatg tttattgaa cggggtatt 
        agaggaggt gtttcacag tgtagtaat agatacgca 
        aaagccaat aatcattac atgaaagat ggatataat 
        tcaaatgaa gaactttca tatctcatg tattttgat 
        gtcaacaat ctctatggt gccgcaatg tctcaatat 
        ttaccttat ggtaacttt agattttta gaagacttt 
        aacattaca gaaatttta aatacatca gatacttcc 
        aatgatggt tatataatt gaatgtgat ttagaatat 
        ccaactcct ttacatgat ttacacagt gacctacct 
        ttagcacca caacattta aaaccacca aattccaaa 
        tcaaaatta gaaaaattg cttttaaca ttgtttcca 
        aaaattaat tatatagtc cattatcgc aatttaaaa 
        atgtattta agacttggt ttaaaaatt actaaagta 
        catcgcgtc ctaacattt aatcagagc ccatggctt 
        aaggaatat atagattta aatacacgc ttaagacag 
        cagtcacaa aacgaattt gaaaaagat ttttttaaa 
        ctaatgatc aatgctata tatggtaaa tgcatggaa 
        aatgtacgt aaacataaa gacattaga attgtaact 
        aggtgggat ggtaggtac ggtatgcgc gatttaatt 
        tcaaaaccc aattttcat agttctgtt atatttgaa 
        gaagacatg tcgatagtt gaaatgaat aaattaaat 
        gtggtacta aataaacca atttatgta ggtttttgt 
        attttagat atttcaaaa atatttatt tacgaattt 
        cattatgac tatattaaa aataagttt ggtaataat 
        gcaaaattg ttgtacaca gacaccgat agtttaata 
        tatcatttt aagactgaa gatatttat aaaaatatt 
        aaggaagat attcatcgc tttgatact tccgattat 
        aaacttgat aataaattt aatattgaa ttaaaaaat 
        aagaaatgt ccagggctc atgaaagat gaaaatcat 
        ggcaaaatt atgcttgaa tttgttggg cttcgatct 
        aaaatgtat ttctataca gttgattca gacactgaa 
        gatgaagag catgattat gatgaagta ggtccagct 
        ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg 
        tgcacaaaa tcttcaata aaacatatt acatttaat 
        gattataaa aaatgttta ttcgattta gatatattt 
        cgtagcacg caaagactc attcgatct aaaaaacac 
        tctgtgtat tctataaaa caagaaaaa gtggtctta 
        agtccatat gatgataaa cgcatatta ttaagaaat 
        actacggat acaagacct tggggatat gctattaat 
        ctaacgtga
      mRNA sequence
      • >TC005292 RA
        atgtctgac acagtgagc tctttagct aaacgttct 
        ataaattta attcgtgaa tacattagt aaaattaaa 
        actttagat gaaattaat tcgttttcg aaatatatt 
        aataggagt gttaaaata gttagcaaa gctttgatc 
        aataaaatt catgaaaca aacattcat gaaaatcca 
        caatctttg gaagccagt cttgctcat ttgaaatac 
        tatcgactc aaattaaaa aaaattaaa aaagactta 
        ctccacaat aaaagcagt aaagttggt cagggatta 
        tcaaatgta ccgaaaaaa acatttggt caaaaggtt 
        ttatgggag gatttaaat tcttgtttt aaaggaaga 
        attcgttca ggttttatt tcaaatatt catataaaa 
        gatccagca atattttta acacgagca tataaaagt 
        tttgtatta aaaattaaa aaagaatta gcaaagtca 
        atgcttaaa gtaaatgta gtgttttca ggtaacttt 
        atacaacca tctacgttg gaaatagat ttaaaaaca 
        tttgcaact agaaataat catatagat tcaacaact 
        aatattaga gaatggtat catgaaaat gtagttagt 
        gtaatatta aataaatta gaatcattt gctgaaaaa 
        gatagtggt tgggcgtta tatgaaatt ttgggatta 
        aaggtaaat attaatcaa tatgatcca ataaaattt 
        ggaaataat tcaagtttt gtggaagta ccgaacttt 
        attaaaaaa aaacattgt ataataaat gtacagaat 
        aatgatcaa ttttgtttt ctttggtca gttgtgtct 
        gcgttatat cctgtaaaa aataatccc cacagaaca 
        tcatcatat ccaaatttt gaaaacgtt ttaaggtat 
        gataatata agttttcca ataacaatt agagatatt 
        gaaaaattt gaaataatg aacaattta tcaattaat 
        ttatattgt attgaagaa aaaaataaa atatttccg 
        gcacgttta agcacttat aataaaaat aatgataag 
        aatattcct acaataaat ttactcatg ctaccaaca 
        aataacatg gatgaagaa gaaacgcat cataataaa 
        aaattatat taccatttt gtatggatt aaacattta 
        tctcgcctt ttatcacat cagttgtct actcatgaa 
        cataaaaca tttatttgt gaacgatgt ttaaattat 
        tttacaacc gaagacatt ctaaccaaa cataaaatt 
        cactgtcaa aagcttaat cagtattca gtgtcatta 
        ccacgcgat acatctttg aaatttaaa aatcatctt 
        tataagaaa aaagtgcca tttgtaatt tatgctgat 
        ctcgaaagt attttggta aaatgttct caaattgat 
        aataatatt cacagtaaa actatccct caccaaaaa 
        catattgct tgtagtata gcttattac ttaaagtgt 
        tcatttgat gactcgctt tcaaaattt caattatat 
        cgtaatcaa gattgtatt cagtggttt gttaaggaa 
        ctaaaagac attgccgaa caatgtgat aaaatattt 
        aaaaataaa ttacccatg gaaaaactt agctacgat 
        caagaacgt tcatataaa gagtccaat gtttgtcac 
        atttgtaat aaaactatt gaatcagat caaattaaa 
        gttcgagat cataatcat ttaacggga aaatttaga 
        ggaccggct catcaacga tgtaattta aattatcaa 
        gattcatat accattcct gttgtgttc cataatttg 
        agtggatat gatgctcat tttattatt aaagaactg 
        tcaacagct tttgccgga ttaactttc gatgcaatg 
        ctgaaatct actggaata tgtttagag ctattaagt 
        gatatagat atgataatg tttattgaa cggggtatt 
        agaggaggt gtttcacag tgtagtaat agatacgca 
        aaagccaat aatcattac atgaaagat ggatataat 
        tcaaatgaa gaactttca tatctcatg tattttgat 
        gtcaacaat ctctatggt gccgcaatg tctcaatat 
        ttaccttat ggtaacttt agattttta gaagacttt 
        aacattaca gaaatttta aatacatca gatacttcc 
        aatgatggt tatataatt gaatgtgat ttagaatat 
        ccaactcct ttacatgat ttacacagt gacctacct 
        ttagcacca caacattta aaaccacca aattccaaa 
        tcaaaatta gaaaaattg cttttaaca ttgtttcca 
        aaaattaat tatatagtc cattatcgc aatttaaaa 
        atgtattta agacttggt ttaaaaatt actaaagta 
        catcgcgtc ctaacattt aatcagagc ccatggctt 
        aaggaatat atagattta aatacacgc ttaagacag 
        cagtcacaa aacgaattt gaaaaagat ttttttaaa 
        ctaatgatc aatgctata tatggtaaa tgcatggaa 
        aatgtacgt aaacataaa gacattaga attgtaact 
        aggtgggat ggtaggtac ggtatgcgc gatttaatt 
        tcaaaaccc aattttcat agttctgtt atatttgaa 
        gaagacatg tcgatagtt gaaatgaat aaattaaat 
        gtggtacta aataaacca atttatgta ggtttttgt 
        attttagat atttcaaaa atatttatt tacgaattt 
        cattatgac tatattaaa aataagttt ggtaataat 
        gcaaaattg ttgtacaca gacaccgat agtttaata 
        tatcatttt aagactgaa gatatttat aaaaatatt 
        aaggaagat attcatcgc tttgatact tccgattat 
        aaacttgat aataaattt aatattgaa ttaaaaaat 
        aagaaatgt ccagggctc atgaaagat gaaaatcat 
        ggcaaaatt atgcttgaa tttgttggg cttcgatct 
        aaaatgtat ttctataca gttgattca gacactgaa 
        gatgaagag catgattat gatgaagta ggtccagct 
        ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg 
        tgcacaaaa tcttcaata aaacatatt acatttaat 
        gattataaa aaatgttta ttcgattta gatatattt 
        cgtagcacg caaagactc attcgatct aaaaaacac 
        tctgtgtat tctataaaa caagaaaaa gtggtctta 
        agtccatat gatgataaa cgcatatta ttaagaaat 
        actacggat acaagacct tggggatat gctattaat 
        ctaacgtga