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Gene information
General information
TC005292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451839.1: 11318..15135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC005292
      iBeetle screen iB_03934

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005292 RA
        MSDTVSSLA KRSINLIRE YISKIKTLD EINSFSKYI 
NRSVKIVSK ALINKIHET NIHENPQSL EASLAHLKY 
YRLKLKKIK KDLLHNKSS KVGQGLSNV PKKTFGQKV 
LWEDLNSCF KGRIRSGFI SNIHIKDPA IFLTRAYKS 
FVLKIKKEL AKSMLKVNV VFSGNFIQP STLEIDLKT 
FATRNNHID STTNIREWY HENVVSVIL NKLESFAEK 
DSGWALYEI LGLKVNINQ YDPIKFGNN SSFVEVPNF 
IKKKHCIIN VQNNDQFCF LWSVVSALY PVKNNPHRT 
SSYPNFENV LRYDNISFP ITIRDIEKF EIMNNLSIN 
LYCIEEKNK IFPARLSTY NKNNDKNIP TINLLMLPT 
NNMDEEETH HNKKLYYHF VWIKHLSRL LSHQLSTHE 
HKTFICERC LNYFTTEDI LTKHKIHCQ KLNQYSVSL 
PRDTSLKFK NHLYKKKVP FVIYADLES ILVKCSQID 
NNIHSKTIP HQKHIACSI AYYLKCSFD DSLSKFQLY 
RNQDCIQWF VKELKDIAE QCDKIFKNK LPMEKLSYD 
QERSYKESN VCHICNKTI ESDQIKVRD HNHLTGKFR 
GPAHQRCNL NYQDSYTIP VVFHNLSGY DAHFIIKEL 
STAFAGLTF DAMLKSTGI CLELLSDID MIMFIERGI 
RGGVSQCSN RYAKANNHY MKDGYNSNE ELSYLMYFD 
VNNLYGAAM SQYLPYGNF RFLEDFNIT EILNTSDTS 
NDGYIIECD LEYPTPLHD LHSDLPLAP QHLKPPNSK 
SKLEKLLLT LFPKINYIV HYRNLKMYL RLGLKITKV 
HRVLTFNQS PWLKEYIDL NTRLRQQSQ NEFEKDFFK 
LMINAIYGK CMENVRKHK DIRIVTRWD GRYGMRDLI 
SKPNFHSSV IFEEDMSIV EMNKLNVVL NKPIYVGFC 
ILDISKIFI YEFHYDYIK NKFGNNAKL LYTDTDSLI 
YHFKTEDIY KNIKEDIHR FDTSDYKLD NKFNIELKN 
KKCPGLMKD ENHGKIMLE FVGLRSKMY FYTVDSDTE 
DEEHDYDEV GPAIKRKKF VKKSKGCTK SSIKHITFN 
DYKKCLFDL DIFRSTQRL IRSKKHSVY SIKQEKVVL 
SPYDDKRIL LRNTTDTRP WGYAINLT
      CDS sequence
      • >TC005292 RA
        atgtctgac acagtgagc tctttagct aaacgttct 
ataaattta attcgtgaa tacattagt aaaattaaa 
actttagat gaaattaat tcgttttcg aaatatatt 
aataggagt gttaaaata gttagcaaa gctttgatc 
aataaaatt catgaaaca aacattcat gaaaatcca 
caatctttg gaagccagt cttgctcat ttgaaatac 
tatcgactc aaattaaaa aaaattaaa aaagactta 
ctccacaat aaaagcagt aaagttggt cagggatta 
tcaaatgta ccgaaaaaa acatttggt caaaaggtt 
ttatgggag gatttaaat tcttgtttt aaaggaaga 
attcgttca ggttttatt tcaaatatt catataaaa 
gatccagca atattttta acacgagca tataaaagt 
tttgtatta aaaattaaa aaagaatta gcaaagtca 
atgcttaaa gtaaatgta gtgttttca ggtaacttt 
atacaacca tctacgttg gaaatagat ttaaaaaca 
tttgcaact agaaataat catatagat tcaacaact 
aatattaga gaatggtat catgaaaat gtagttagt 
gtaatatta aataaatta gaatcattt gctgaaaaa 
gatagtggt tgggcgtta tatgaaatt ttgggatta 
aaggtaaat attaatcaa tatgatcca ataaaattt 
ggaaataat tcaagtttt gtggaagta ccgaacttt 
attaaaaaa aaacattgt ataataaat gtacagaat 
aatgatcaa ttttgtttt ctttggtca gttgtgtct 
gcgttatat cctgtaaaa aataatccc cacagaaca 
tcatcatat ccaaatttt gaaaacgtt ttaaggtat 
gataatata agttttcca ataacaatt agagatatt 
gaaaaattt gaaataatg aacaattta tcaattaat 
ttatattgt attgaagaa aaaaataaa atatttccg 
gcacgttta agcacttat aataaaaat aatgataag 
aatattcct acaataaat ttactcatg ctaccaaca 
aataacatg gatgaagaa gaaacgcat cataataaa 
aaattatat taccatttt gtatggatt aaacattta 
tctcgcctt ttatcacat cagttgtct actcatgaa 
cataaaaca tttatttgt gaacgatgt ttaaattat 
tttacaacc gaagacatt ctaaccaaa cataaaatt 
cactgtcaa aagcttaat cagtattca gtgtcatta 
ccacgcgat acatctttg aaatttaaa aatcatctt 
tataagaaa aaagtgcca tttgtaatt tatgctgat 
ctcgaaagt attttggta aaatgttct caaattgat 
aataatatt cacagtaaa actatccct caccaaaaa 
catattgct tgtagtata gcttattac ttaaagtgt 
tcatttgat gactcgctt tcaaaattt caattatat 
cgtaatcaa gattgtatt cagtggttt gttaaggaa 
ctaaaagac attgccgaa caatgtgat aaaatattt 
aaaaataaa ttacccatg gaaaaactt agctacgat 
caagaacgt tcatataaa gagtccaat gtttgtcac 
atttgtaat aaaactatt gaatcagat caaattaaa 
gttcgagat cataatcat ttaacggga aaatttaga 
ggaccggct catcaacga tgtaattta aattatcaa 
gattcatat accattcct gttgtgttc cataatttg 
agtggatat gatgctcat tttattatt aaagaactg 
tcaacagct tttgccgga ttaactttc gatgcaatg 
ctgaaatct actggaata tgtttagag ctattaagt 
gatatagat atgataatg tttattgaa cggggtatt 
agaggaggt gtttcacag tgtagtaat agatacgca 
aaagccaat aatcattac atgaaagat ggatataat 
tcaaatgaa gaactttca tatctcatg tattttgat 
gtcaacaat ctctatggt gccgcaatg tctcaatat 
ttaccttat ggtaacttt agattttta gaagacttt 
aacattaca gaaatttta aatacatca gatacttcc 
aatgatggt tatataatt gaatgtgat ttagaatat 
ccaactcct ttacatgat ttacacagt gacctacct 
ttagcacca caacattta aaaccacca aattccaaa 
tcaaaatta gaaaaattg cttttaaca ttgtttcca 
aaaattaat tatatagtc cattatcgc aatttaaaa 
atgtattta agacttggt ttaaaaatt actaaagta 
catcgcgtc ctaacattt aatcagagc ccatggctt 
aaggaatat atagattta aatacacgc ttaagacag 
cagtcacaa aacgaattt gaaaaagat ttttttaaa 
ctaatgatc aatgctata tatggtaaa tgcatggaa 
aatgtacgt aaacataaa gacattaga attgtaact 
aggtgggat ggtaggtac ggtatgcgc gatttaatt 
tcaaaaccc aattttcat agttctgtt atatttgaa 
gaagacatg tcgatagtt gaaatgaat aaattaaat 
gtggtacta aataaacca atttatgta ggtttttgt 
attttagat atttcaaaa atatttatt tacgaattt 
cattatgac tatattaaa aataagttt ggtaataat 
gcaaaattg ttgtacaca gacaccgat agtttaata 
tatcatttt aagactgaa gatatttat aaaaatatt 
aaggaagat attcatcgc tttgatact tccgattat 
aaacttgat aataaattt aatattgaa ttaaaaaat 
aagaaatgt ccagggctc atgaaagat gaaaatcat 
ggcaaaatt atgcttgaa tttgttggg cttcgatct 
aaaatgtat ttctataca gttgattca gacactgaa 
gatgaagag catgattat gatgaagta ggtccagct 
ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg 
tgcacaaaa tcttcaata aaacatatt acatttaat 
gattataaa aaatgttta ttcgattta gatatattt 
cgtagcacg caaagactc attcgatct aaaaaacac 
tctgtgtat tctataaaa caagaaaaa gtggtctta 
agtccatat gatgataaa cgcatatta ttaagaaat 
actacggat acaagacct tggggatat gctattaat 
ctaacgtga
      mRNA sequence
      • >TC005292 RA
        atgtctgac acagtgagc tctttagct aaacgttct 
ataaattta attcgtgaa tacattagt aaaattaaa 
actttagat gaaattaat tcgttttcg aaatatatt 
aataggagt gttaaaata gttagcaaa gctttgatc 
aataaaatt catgaaaca aacattcat gaaaatcca 
caatctttg gaagccagt cttgctcat ttgaaatac 
tatcgactc aaattaaaa aaaattaaa aaagactta 
ctccacaat aaaagcagt aaagttggt cagggatta 
tcaaatgta ccgaaaaaa acatttggt caaaaggtt 
ttatgggag gatttaaat tcttgtttt aaaggaaga 
attcgttca ggttttatt tcaaatatt catataaaa 
gatccagca atattttta acacgagca tataaaagt 
tttgtatta aaaattaaa aaagaatta gcaaagtca 
atgcttaaa gtaaatgta gtgttttca ggtaacttt 
atacaacca tctacgttg gaaatagat ttaaaaaca 
tttgcaact agaaataat catatagat tcaacaact 
aatattaga gaatggtat catgaaaat gtagttagt 
gtaatatta aataaatta gaatcattt gctgaaaaa 
gatagtggt tgggcgtta tatgaaatt ttgggatta 
aaggtaaat attaatcaa tatgatcca ataaaattt 
ggaaataat tcaagtttt gtggaagta ccgaacttt 
attaaaaaa aaacattgt ataataaat gtacagaat 
aatgatcaa ttttgtttt ctttggtca gttgtgtct 
gcgttatat cctgtaaaa aataatccc cacagaaca 
tcatcatat ccaaatttt gaaaacgtt ttaaggtat 
gataatata agttttcca ataacaatt agagatatt 
gaaaaattt gaaataatg aacaattta tcaattaat 
ttatattgt attgaagaa aaaaataaa atatttccg 
gcacgttta agcacttat aataaaaat aatgataag 
aatattcct acaataaat ttactcatg ctaccaaca 
aataacatg gatgaagaa gaaacgcat cataataaa 
aaattatat taccatttt gtatggatt aaacattta 
tctcgcctt ttatcacat cagttgtct actcatgaa 
cataaaaca tttatttgt gaacgatgt ttaaattat 
tttacaacc gaagacatt ctaaccaaa cataaaatt 
cactgtcaa aagcttaat cagtattca gtgtcatta 
ccacgcgat acatctttg aaatttaaa aatcatctt 
tataagaaa aaagtgcca tttgtaatt tatgctgat 
ctcgaaagt attttggta aaatgttct caaattgat 
aataatatt cacagtaaa actatccct caccaaaaa 
catattgct tgtagtata gcttattac ttaaagtgt 
tcatttgat gactcgctt tcaaaattt caattatat 
cgtaatcaa gattgtatt cagtggttt gttaaggaa 
ctaaaagac attgccgaa caatgtgat aaaatattt 
aaaaataaa ttacccatg gaaaaactt agctacgat 
caagaacgt tcatataaa gagtccaat gtttgtcac 
atttgtaat aaaactatt gaatcagat caaattaaa 
gttcgagat cataatcat ttaacggga aaatttaga 
ggaccggct catcaacga tgtaattta aattatcaa 
gattcatat accattcct gttgtgttc cataatttg 
agtggatat gatgctcat tttattatt aaagaactg 
tcaacagct tttgccgga ttaactttc gatgcaatg 
ctgaaatct actggaata tgtttagag ctattaagt 
gatatagat atgataatg tttattgaa cggggtatt 
agaggaggt gtttcacag tgtagtaat agatacgca 
aaagccaat aatcattac atgaaagat ggatataat 
tcaaatgaa gaactttca tatctcatg tattttgat 
gtcaacaat ctctatggt gccgcaatg tctcaatat 
ttaccttat ggtaacttt agattttta gaagacttt 
aacattaca gaaatttta aatacatca gatacttcc 
aatgatggt tatataatt gaatgtgat ttagaatat 
ccaactcct ttacatgat ttacacagt gacctacct 
ttagcacca caacattta aaaccacca aattccaaa 
tcaaaatta gaaaaattg cttttaaca ttgtttcca 
aaaattaat tatatagtc cattatcgc aatttaaaa 
atgtattta agacttggt ttaaaaatt actaaagta 
catcgcgtc ctaacattt aatcagagc ccatggctt 
aaggaatat atagattta aatacacgc ttaagacag 
cagtcacaa aacgaattt gaaaaagat ttttttaaa 
ctaatgatc aatgctata tatggtaaa tgcatggaa 
aatgtacgt aaacataaa gacattaga attgtaact 
aggtgggat ggtaggtac ggtatgcgc gatttaatt 
tcaaaaccc aattttcat agttctgtt atatttgaa 
gaagacatg tcgatagtt gaaatgaat aaattaaat 
gtggtacta aataaacca atttatgta ggtttttgt 
attttagat atttcaaaa atatttatt tacgaattt 
cattatgac tatattaaa aataagttt ggtaataat 
gcaaaattg ttgtacaca gacaccgat agtttaata 
tatcatttt aagactgaa gatatttat aaaaatatt 
aaggaagat attcatcgc tttgatact tccgattat 
aaacttgat aataaattt aatattgaa ttaaaaaat 
aagaaatgt ccagggctc atgaaagat gaaaatcat 
ggcaaaatt atgcttgaa tttgttggg cttcgatct 
aaaatgtat ttctataca gttgattca gacactgaa 
gatgaagag catgattat gatgaagta ggtccagct 
ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg 
tgcacaaaa tcttcaata aaacatatt acatttaat 
gattataaa aaatgttta ttcgattta gatatattt 
cgtagcacg caaagactc attcgatct aaaaaacac 
tctgtgtat tctataaaa caagaaaaa gtggtctta 
agtccatat gatgataaa cgcatatta ttaagaaat 
actacggat acaagacct tggggatat gctattaat 
ctaacgtga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY