| General information | |||
| TC005292 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451839.1: 11318..15135 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005292 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03934 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC005292 RA
MSDTVSSLA KRSINLIRE YISKIKTLD EINSFSKYI NRSVKIVSK ALINKIHET NIHENPQSL EASLAHLKY YRLKLKKIK KDLLHNKSS KVGQGLSNV PKKTFGQKV LWEDLNSCF KGRIRSGFI SNIHIKDPA IFLTRAYKS FVLKIKKEL AKSMLKVNV VFSGNFIQP STLEIDLKT FATRNNHID STTNIREWY HENVVSVIL NKLESFAEK DSGWALYEI LGLKVNINQ YDPIKFGNN SSFVEVPNF IKKKHCIIN VQNNDQFCF LWSVVSALY PVKNNPHRT SSYPNFENV LRYDNISFP ITIRDIEKF EIMNNLSIN LYCIEEKNK IFPARLSTY NKNNDKNIP TINLLMLPT NNMDEEETH HNKKLYYHF VWIKHLSRL LSHQLSTHE HKTFICERC LNYFTTEDI LTKHKIHCQ KLNQYSVSL PRDTSLKFK NHLYKKKVP FVIYADLES ILVKCSQID NNIHSKTIP HQKHIACSI AYYLKCSFD DSLSKFQLY RNQDCIQWF VKELKDIAE QCDKIFKNK LPMEKLSYD QERSYKESN VCHICNKTI ESDQIKVRD HNHLTGKFR GPAHQRCNL NYQDSYTIP VVFHNLSGY DAHFIIKEL STAFAGLTF DAMLKSTGI CLELLSDID MIMFIERGI RGGVSQCSN RYAKANNHY MKDGYNSNE ELSYLMYFD VNNLYGAAM SQYLPYGNF RFLEDFNIT EILNTSDTS NDGYIIECD LEYPTPLHD LHSDLPLAP QHLKPPNSK SKLEKLLLT LFPKINYIV HYRNLKMYL RLGLKITKV HRVLTFNQS PWLKEYIDL NTRLRQQSQ NEFEKDFFK LMINAIYGK CMENVRKHK DIRIVTRWD GRYGMRDLI SKPNFHSSV IFEEDMSIV EMNKLNVVL NKPIYVGFC ILDISKIFI YEFHYDYIK NKFGNNAKL LYTDTDSLI YHFKTEDIY KNIKEDIHR FDTSDYKLD NKFNIELKN KKCPGLMKD ENHGKIMLE FVGLRSKMY FYTVDSDTE DEEHDYDEV GPAIKRKKF VKKSKGCTK SSIKHITFN DYKKCLFDL DIFRSTQRL IRSKKHSVY SIKQEKVVL SPYDDKRIL LRNTTDTRP WGYAINLT
- >TC005292 RA
atgtctgac acagtgagc tctttagct aaacgttct ataaattta attcgtgaa tacattagt aaaattaaa actttagat gaaattaat tcgttttcg aaatatatt aataggagt gttaaaata gttagcaaa gctttgatc aataaaatt catgaaaca aacattcat gaaaatcca caatctttg gaagccagt cttgctcat ttgaaatac tatcgactc aaattaaaa aaaattaaa aaagactta ctccacaat aaaagcagt aaagttggt cagggatta tcaaatgta ccgaaaaaa acatttggt caaaaggtt ttatgggag gatttaaat tcttgtttt aaaggaaga attcgttca ggttttatt tcaaatatt catataaaa gatccagca atattttta acacgagca tataaaagt tttgtatta aaaattaaa aaagaatta gcaaagtca atgcttaaa gtaaatgta gtgttttca ggtaacttt atacaacca tctacgttg gaaatagat ttaaaaaca tttgcaact agaaataat catatagat tcaacaact aatattaga gaatggtat catgaaaat gtagttagt gtaatatta aataaatta gaatcattt gctgaaaaa gatagtggt tgggcgtta tatgaaatt ttgggatta aaggtaaat attaatcaa tatgatcca ataaaattt ggaaataat tcaagtttt gtggaagta ccgaacttt attaaaaaa aaacattgt ataataaat gtacagaat aatgatcaa ttttgtttt ctttggtca gttgtgtct gcgttatat cctgtaaaa aataatccc cacagaaca tcatcatat ccaaatttt gaaaacgtt ttaaggtat gataatata agttttcca ataacaatt agagatatt gaaaaattt gaaataatg aacaattta tcaattaat ttatattgt attgaagaa aaaaataaa atatttccg gcacgttta agcacttat aataaaaat aatgataag aatattcct acaataaat ttactcatg ctaccaaca aataacatg gatgaagaa gaaacgcat cataataaa aaattatat taccatttt gtatggatt aaacattta tctcgcctt ttatcacat cagttgtct actcatgaa cataaaaca tttatttgt gaacgatgt ttaaattat tttacaacc gaagacatt ctaaccaaa cataaaatt cactgtcaa aagcttaat cagtattca gtgtcatta ccacgcgat acatctttg aaatttaaa aatcatctt tataagaaa aaagtgcca tttgtaatt tatgctgat ctcgaaagt attttggta aaatgttct caaattgat aataatatt cacagtaaa actatccct caccaaaaa catattgct tgtagtata gcttattac ttaaagtgt tcatttgat gactcgctt tcaaaattt caattatat cgtaatcaa gattgtatt cagtggttt gttaaggaa ctaaaagac attgccgaa caatgtgat aaaatattt aaaaataaa ttacccatg gaaaaactt agctacgat caagaacgt tcatataaa gagtccaat gtttgtcac atttgtaat aaaactatt gaatcagat caaattaaa gttcgagat cataatcat ttaacggga aaatttaga ggaccggct catcaacga tgtaattta aattatcaa gattcatat accattcct gttgtgttc cataatttg agtggatat gatgctcat tttattatt aaagaactg tcaacagct tttgccgga ttaactttc gatgcaatg ctgaaatct actggaata tgtttagag ctattaagt gatatagat atgataatg tttattgaa cggggtatt agaggaggt gtttcacag tgtagtaat agatacgca aaagccaat aatcattac atgaaagat ggatataat tcaaatgaa gaactttca tatctcatg tattttgat gtcaacaat ctctatggt gccgcaatg tctcaatat ttaccttat ggtaacttt agattttta gaagacttt aacattaca gaaatttta aatacatca gatacttcc aatgatggt tatataatt gaatgtgat ttagaatat ccaactcct ttacatgat ttacacagt gacctacct ttagcacca caacattta aaaccacca aattccaaa tcaaaatta gaaaaattg cttttaaca ttgtttcca aaaattaat tatatagtc cattatcgc aatttaaaa atgtattta agacttggt ttaaaaatt actaaagta catcgcgtc ctaacattt aatcagagc ccatggctt aaggaatat atagattta aatacacgc ttaagacag cagtcacaa aacgaattt gaaaaagat ttttttaaa ctaatgatc aatgctata tatggtaaa tgcatggaa aatgtacgt aaacataaa gacattaga attgtaact aggtgggat ggtaggtac ggtatgcgc gatttaatt tcaaaaccc aattttcat agttctgtt atatttgaa gaagacatg tcgatagtt gaaatgaat aaattaaat gtggtacta aataaacca atttatgta ggtttttgt attttagat atttcaaaa atatttatt tacgaattt cattatgac tatattaaa aataagttt ggtaataat gcaaaattg ttgtacaca gacaccgat agtttaata tatcatttt aagactgaa gatatttat aaaaatatt aaggaagat attcatcgc tttgatact tccgattat aaacttgat aataaattt aatattgaa ttaaaaaat aagaaatgt ccagggctc atgaaagat gaaaatcat ggcaaaatt atgcttgaa tttgttggg cttcgatct aaaatgtat ttctataca gttgattca gacactgaa gatgaagag catgattat gatgaagta ggtccagct ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg tgcacaaaa tcttcaata aaacatatt acatttaat gattataaa aaatgttta ttcgattta gatatattt cgtagcacg caaagactc attcgatct aaaaaacac tctgtgtat tctataaaa caagaaaaa gtggtctta agtccatat gatgataaa cgcatatta ttaagaaat actacggat acaagacct tggggatat gctattaat ctaacgtga
- >TC005292 RA
atgtctgac acagtgagc tctttagct aaacgttct ataaattta attcgtgaa tacattagt aaaattaaa actttagat gaaattaat tcgttttcg aaatatatt aataggagt gttaaaata gttagcaaa gctttgatc aataaaatt catgaaaca aacattcat gaaaatcca caatctttg gaagccagt cttgctcat ttgaaatac tatcgactc aaattaaaa aaaattaaa aaagactta ctccacaat aaaagcagt aaagttggt cagggatta tcaaatgta ccgaaaaaa acatttggt caaaaggtt ttatgggag gatttaaat tcttgtttt aaaggaaga attcgttca ggttttatt tcaaatatt catataaaa gatccagca atattttta acacgagca tataaaagt tttgtatta aaaattaaa aaagaatta gcaaagtca atgcttaaa gtaaatgta gtgttttca ggtaacttt atacaacca tctacgttg gaaatagat ttaaaaaca tttgcaact agaaataat catatagat tcaacaact aatattaga gaatggtat catgaaaat gtagttagt gtaatatta aataaatta gaatcattt gctgaaaaa gatagtggt tgggcgtta tatgaaatt ttgggatta aaggtaaat attaatcaa tatgatcca ataaaattt ggaaataat tcaagtttt gtggaagta ccgaacttt attaaaaaa aaacattgt ataataaat gtacagaat aatgatcaa ttttgtttt ctttggtca gttgtgtct gcgttatat cctgtaaaa aataatccc cacagaaca tcatcatat ccaaatttt gaaaacgtt ttaaggtat gataatata agttttcca ataacaatt agagatatt gaaaaattt gaaataatg aacaattta tcaattaat ttatattgt attgaagaa aaaaataaa atatttccg gcacgttta agcacttat aataaaaat aatgataag aatattcct acaataaat ttactcatg ctaccaaca aataacatg gatgaagaa gaaacgcat cataataaa aaattatat taccatttt gtatggatt aaacattta tctcgcctt ttatcacat cagttgtct actcatgaa cataaaaca tttatttgt gaacgatgt ttaaattat tttacaacc gaagacatt ctaaccaaa cataaaatt cactgtcaa aagcttaat cagtattca gtgtcatta ccacgcgat acatctttg aaatttaaa aatcatctt tataagaaa aaagtgcca tttgtaatt tatgctgat ctcgaaagt attttggta aaatgttct caaattgat aataatatt cacagtaaa actatccct caccaaaaa catattgct tgtagtata gcttattac ttaaagtgt tcatttgat gactcgctt tcaaaattt caattatat cgtaatcaa gattgtatt cagtggttt gttaaggaa ctaaaagac attgccgaa caatgtgat aaaatattt aaaaataaa ttacccatg gaaaaactt agctacgat caagaacgt tcatataaa gagtccaat gtttgtcac atttgtaat aaaactatt gaatcagat caaattaaa gttcgagat cataatcat ttaacggga aaatttaga ggaccggct catcaacga tgtaattta aattatcaa gattcatat accattcct gttgtgttc cataatttg agtggatat gatgctcat tttattatt aaagaactg tcaacagct tttgccgga ttaactttc gatgcaatg ctgaaatct actggaata tgtttagag ctattaagt gatatagat atgataatg tttattgaa cggggtatt agaggaggt gtttcacag tgtagtaat agatacgca aaagccaat aatcattac atgaaagat ggatataat tcaaatgaa gaactttca tatctcatg tattttgat gtcaacaat ctctatggt gccgcaatg tctcaatat ttaccttat ggtaacttt agattttta gaagacttt aacattaca gaaatttta aatacatca gatacttcc aatgatggt tatataatt gaatgtgat ttagaatat ccaactcct ttacatgat ttacacagt gacctacct ttagcacca caacattta aaaccacca aattccaaa tcaaaatta gaaaaattg cttttaaca ttgtttcca aaaattaat tatatagtc cattatcgc aatttaaaa atgtattta agacttggt ttaaaaatt actaaagta catcgcgtc ctaacattt aatcagagc ccatggctt aaggaatat atagattta aatacacgc ttaagacag cagtcacaa aacgaattt gaaaaagat ttttttaaa ctaatgatc aatgctata tatggtaaa tgcatggaa aatgtacgt aaacataaa gacattaga attgtaact aggtgggat ggtaggtac ggtatgcgc gatttaatt tcaaaaccc aattttcat agttctgtt atatttgaa gaagacatg tcgatagtt gaaatgaat aaattaaat gtggtacta aataaacca atttatgta ggtttttgt attttagat atttcaaaa atatttatt tacgaattt cattatgac tatattaaa aataagttt ggtaataat gcaaaattg ttgtacaca gacaccgat agtttaata tatcatttt aagactgaa gatatttat aaaaatatt aaggaagat attcatcgc tttgatact tccgattat aaacttgat aataaattt aatattgaa ttaaaaaat aagaaatgt ccagggctc atgaaagat gaaaatcat ggcaaaatt atgcttgaa tttgttggg cttcgatct aaaatgtat ttctataca gttgattca gacactgaa gatgaagag catgattat gatgaagta ggtccagct ataaaacgt aaaaaattt gtgaaaaag tcgaaaggg tgcacaaaa tcttcaata aaacatatt acatttaat gattataaa aaatgttta ttcgattta gatatattt cgtagcacg caaagactc attcgatct aaaaaacac tctgtgtat tctataaaa caagaaaaa gtggtctta agtccatat gatgataaa cgcatatta ttaagaaat actacggat acaagacct tggggatat gctattaat ctaacgtga
