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Gene information
General information
TC005274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid592: 48730..52047
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005274
      iBeetle screen iB_03932
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      iBeetle screen iB_05706
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005274 RA
        MAVNSIKIA TANSGGVTR KKAAIENFL FRDNIEILG 
        ITEVKAKSF PKFRGFNAI HRRYPAQQG QRETRGVAI 
        LIKKGFAFS EHQLPNNLQ HLDCIAVDT YIDSNIITF 
        FTYYNSPRQ CLSNELMQY AASLPKAII LGDFNARHT 
        DFGDTLTNR NGHILIDAI SNLPLYRIP NSSPTHINH 
        WEGYSIVDH ILVTDSLSN RASDSANIG TTITSDHLP 
        LVFDLSVSK PRTPTPEYI IIRDSKNTD WEKFRIILE 
        KEIVPLDPT TTPEEIERQ VFYISELIQ DAYLQCTPE 
        KQIPLHKTP LPPEIVDKI RLKRQVYRE YINTRDPDI 
        KTQWNRLNA TIRRDINRH REKLWRETC SGLNYRDGK 
        RFWNKLKIL TGQKSNPRH FLKVNNQFI TTDTDKANA 
        YAKHLEKIF QIPEDPLFN DSHKKRVET FHSQFIHRT 
        QARTCYITQ ADRHKDTQT DDVSTVLEP IQLEEIATK 
        IHLLKNNKA PGNDNIKNI TLKHLPHSV LQAIVQIFN 
        NCLSTSYFP CLWKSGIAI MIQKPGKPP DDPNSYRPV 
        TLLSGIGKL FERIITERL RNFCEGNSL FPPTQFGFR 
        AQRSTCDPL TALQTDVAR HLNLGQCVL GVFLDIEKA 
        FDKVWHAGL IYKLKQLHF SDQLTQLIH SFLSHRTLK 
        VREGNELSN PISINAGVP QGSVLSPLL YLIYCHDIP 
        IKSQIATKT ILFADDTAL WTGQKTSFK ATQVLQECL 
        DEFQKWASK WRIKPNPTK SSAILFRNG HQCQSWKFQ 
        TRNIHLSLW NIQIPLSNE VRYLGISFH KYGNFSSDL 
        NATLRKVRQ RAGIISRLR GGLQGCSSE TLHHTYKTF 
        IRPVIEFRA VLYVSLNSP EIERLISCE RKILRKIFD 
        LYFKFPSER LYEHIGAHS INERLKKLQ SSYVLRTLR 
        SQNEIAKQT LRTSWTDPH NKNLSTSQT FPSKPKMKK 
        VHLPSALLE ITARENELS ASLENIIEE TPLSIRVK
      CDS sequence
      • >TC005274 RA
        atggcggtt aattccatc aaaatcgcc acggccaac 
        tccgggggg gtcacccgg aagaaagcc gcaatagaa 
        aatttcctt ttccgggat aatattgaa attctgggt 
        atcaccgaa gttaaggcc aaatccttt ccaaaattc 
        agaggcttc aatgccatc cacaggaga tacccagct 
        caacagggt caacgcgaa actcgagga gtagccatt 
        ctaatcaaa aaaggtttt gccttcagc gaacaccaa 
        ctccccaac aacttacaa cacttggat tgcatcgcg 
        gtcgacact tacattgac tccaacatc attacattc 
        tttacctac tacaattcc ccaagacag tgtctaagc 
        aacgaatta atgcagtac gccgcatct ctccccaaa 
        gccatcatc ctaggcgat tttaatgct agacatact 
        gattttggc gacacttta accaatcga aacggccac 
        attctcatc gatgcaatc agtaatttg cccctgtac 
        agaattccc aactcttcc ccgacccac atcaatcat 
        tgggaagga tactctatc gtagatcac atcctcgtc 
        acagattcc ttgtccaac cgtgctagc gactctgct 
        aacatcgga acaacaatt acgtcagac caccttcca 
        ctagtattt gatctatct gtatccaag ccaagaacc 
        ccaactcca gagtacatc atcattcga gattccaaa 
        aacacagat tgggaaaag tttcgtatc atcctggaa 
        aaggaaatt gtacctctt gacccaacc accaccccc 
        gaggagatc gagcgccag gtcttctac atatcagaa 
        ctaatacaa gacgcatat cttcaatgc actcccgaa 
        aaacaaatt ccattgcac aaaaccccg ctcccccct 
        gaaattgtc gacaaaatt cggctgaaa cgacaagtt 
        tacagagaa tacattaac acccgcgat ccggacatt 
        aagacacaa tggaaccga cttaatgcc acaattcga 
        cgcgacatt aatcgtcat cgagagaaa ctttggaga 
        gaaacatgt tcaggactc aactaccgc gacgggaaa 
        cgcttttgg aacaaatta aaaatctta acgggacaa 
        aaatcaaac ccacgacac tttttgaaa gttaacaat 
        caattcatc acaactgac acagacaaa gcaaacgct 
        tacgcaaaa cacttagag aaaattttc caaattcct 
        gaagatccg ttgttcaat gactctcat aaaaaacgg 
        gtcgagaca ttccactcg cagttcatt caccgcacc 
        caagcacgc acctgctac ataacacaa gccgaccgc 
        cacaaggac acacaaaca gatgacgtc agcaccgta 
        ctcgaaccc attcaatta gaggaaatc gcgacaaaa 
        atacactta ctaaaaaac aacaaagca cccggaaat 
        gataacatt aaaaacatt acactcaaa caccttcca 
        cactctgtc ttgcaggca attgttcaa attttcaac 
        aattgtctt tctacgtct tactttcct tgtctctgg 
        aaatctgga atcgcaatt atgatccaa aaacccgga 
        aaaccccca gacgaccca aactcttat aggccagtc 
        acacttctg agtggaatt ggaaaactt tttgagaga 
        ataatcacc gaaagactt cgtaatttc tgcgaaggc 
        aactcgctc ttccccccc acgcagttt ggattccgt 
        gcacaaagg tcaacgtgc gacccactt acggcgctg 
        caaacagat gttgcgcgg cacttgaac ctaggtcag 
        tgtgtcttg ggagtcttt ctcgacatc gaaaaagct 
        ttcgataaa gtttggcac gctggattg atttacaaa 
        ttaaaacaa ttacacttt agcgaccaa ttaacacaa 
        ctaatacac tcgttttta tcgcacaga acactaaaa 
        gttcgagaa ggaaatgaa ctatctaac ccaatcagt 
        ataaacgcc ggggttccc caaggttct gttctgtcc 
        cctcttctc tacctaatc tactgtcat gatatacca 
        atcaaatct caaattgct acaaaaacc attctcttt 
        gctgatgac acggccctg tggacagga cagaaaaca 
        tctttcaag gccactcaa gtccttcaa gagtgcctg 
        gatgaattc caaaagtgg gcttccaaa tggaggata 
        aaacctaac ccaaccaag tcttcggcc attctcttc 
        cgaaacgga caccaatgc cagtcgtgg aaatttcaa 
        acccggaac atccacctt tctctttgg aatatccaa 
        attccccta agcaacgag gtccgatat ctaggtatc 
        tcattccat aaatatgga aacttttct tctgacttg 
        aatgctacc cttcgaaag gtgaggcaa agagcggga 
        attatttct aggttacgg ggagggttg caaggctgt 
        tcttccgaa accttgcac cacacatat aaaacattc 
        atacggccg gtcatcgaa ttccgtgct gtactctat 
        gtgtccctc aactctcct gaaatcgaa agactcatc 
        tcttgcgaa aggaagatc ttgcgtaaa atcttcgac 
        ttatatttc aaattcccg tccgaacga ctgtatgaa 
        cacattggc gcacactcg atcaatgag cgtttaaaa 
        aagctccag tcgtcttac gtacttcgt acgctccgg 
        tcccaaaat gaaatagca aagcaaacg ctccgtaca 
        tcttggact gacccccac aataaaaat ctctccact 
        tctcaaacc tttccaagc aaacccaaa atgaaaaaa 
        gtccacctt ccgagcgct ttgctagaa ataacagcc 
        cgtgaaaac gagctgtcg gccagtctg gaaaacatc 
        atcgaagaa acaccactt tccattaga gtcaaataa
      mRNA sequence
      • >TC005274 RA
        aagagaagt taaatacaa tttttctgg ggacatgct 
        tcatgtctc aattgacaa gttgagtac ccacaaaaa 
        tggcggtta attccatca aaatcgcca cggccaact 
        ccggggggg tcacccgga agaaagccg caatagaaa 
        atttccttt tccgggata atattgaaa ttctgggta 
        tcaccgaag ttaaggcca aatcctttc caaaattca 
        gaggcttca atgccatcc acaggagat acccagctc 
        aacagggtc aacgcgaaa ctcgaggag tagccattc 
        taatcaaaa aaggttttg ccttcagcg aacaccaac 
        tccccaaca acttacaac acttggatt gcatcgcgg 
        tcgacactt acattgact ccaacatca ttacattct 
        ttacctact acaattccc caagacagt gtctaagca 
        acgaattaa tgcagtacg ccgcatctc tccccaaag 
        ccatcatcc taggcgatt ttaatgcta gacatactg 
        attttggcg acactttaa ccaatcgaa acggccaca 
        ttctcatcg atgcaatca gtaatttgc ccctgtaca 
        gaattccca actcttccc cgacccaca tcaatcatt 
        gggaaggat actctatcg tagatcaca tcctcgtca 
        cagattcct tgtccaacc gtgctagcg actctgcta 
        acatcggaa caacaatta cgtcagacc accttccac 
        tagtatttg atctatctg tatccaagc caagaaccc 
        caactccag agtacatca tcattcgag attccaaaa 
        acacagatt gggaaaagt ttcgtatca tcctggaaa 
        aggaaattg tacctcttg acccaacca ccacccccg 
        aggagatcg agcgccagg tcttctaca tatcagaac 
        taatacaag acgcatatc ttcaatgca ctcccgaaa 
        aacaaattc cattgcaca aaaccccgc tcccccctg 
        aaattgtcg acaaaattc ggctgaaac gacaagttt 
        acagagaat acattaaca cccgcgatc cggacatta 
        agacacaat ggaaccgac ttaatgcca caattcgac 
        gcgacatta atcgtcatc gagagaaac tttggagag 
        aaacatgtt caggactca actaccgcg acgggaaac 
        gcttttgga acaaattaa aaatcttaa cgggacaaa 
        aatcaaacc cacgacact ttttgaaag ttaacaatc 
        aattcatca caactgaca cagacaaag caaacgctt 
        acgcaaaac acttagaga aaattttcc aaattcctg 
        aagatccgt tgttcaatg actctcata aaaaacggg 
        tcgagacat tccactcgc agttcattc accgcaccc 
        aagcacgca cctgctaca taacacaag ccgaccgcc 
        acaaggaca cacaaacag atgacgtca gcaccgtac 
        tcgaaccca ttcaattag aggaaatcg cgacaaaaa 
        tacacttac taaaaaaca acaaagcac ccggaaatg 
        ataacatta aaaacatta cactcaaac accttccac 
        actctgtct tgcaggcaa ttgttcaaa ttttcaaca 
        attgtcttt ctacgtctt actttcctt gtctctgga 
        aatctggaa tcgcaatta tgatccaaa aacccggaa 
        aacccccag acgacccaa actcttata ggccagtca 
        cacttctga gtggaattg gaaaacttt ttgagagaa 
        taatcaccg aaagacttc gtaatttct gcgaaggca 
        actcgctct tccccccca cgcagtttg gattccgtg 
        cacaaaggt caacgtgcg acccactta cggcgctgc 
        aaacagatg ttgcgcggc acttgaacc taggtcagt 
        gtgtcttgg gagtctttc tcgacatcg aaaaagctt 
        tcgataaag tttggcacg ctggattga tttacaaat 
        taaaacaat tacacttta gcgaccaat taacacaac 
        taatacact cgtttttat cgcacagaa cactaaaag 
        ttcgagaag gaaatgaac tatctaacc caatcagta 
        taaacgccg gggttcccc aaggttctg ttctgtccc 
        ctcttctct acctaatct actgtcatg atataccaa 
        tcaaatctc aaattgcta caaaaacca ttctctttg 
        ctgatgaca cggccctgt ggacaggac agaaaacat 
        ctttcaagg ccactcaag tccttcaag agtgcctgg 
        atgaattcc aaaagtggg cttccaaat ggaggataa 
        aacctaacc caaccaagt cttcggcca ttctcttcc 
        gaaacggac accaatgcc agtcgtgga aatttcaaa 
        cccggaaca tccaccttt ctctttgga atatccaaa 
        ttcccctaa gcaacgagg tccgatatc taggtatct 
        cattccata aatatggaa acttttctt ctgacttga 
        atgctaccc ttcgaaagg tgaggcaaa gagcgggaa 
        ttatttcta ggttacggg gagggttgc aaggctgtt 
        cttccgaaa ccttgcacc acacatata aaacattca 
        tacggccgg tcatcgaat tccgtgctg tactctatg 
        tgtccctca actctcctg aaatcgaaa gactcatct 
        cttgcgaaa ggaagatct tgcgtaaaa tcttcgact 
        tatatttca aattcccgt ccgaacgac tgtatgaac 
        acattggcg cacactcga tcaatgagc gtttaaaaa 
        agctccagt cgtcttacg tacttcgta cgctccggt 
        cccaaaatg aaatagcaa agcaaacgc tccgtacat 
        cttggactg acccccaca ataaaaatc tctccactt 
        ctcaaacct ttccaagca aacccaaaa tgaaaaaag 
        tccaccttc cgagcgctt tgctagaaa taacagccc 
        gtgaaaacg agctgtcgg ccagtctgg aaaacatca 
        tcgaagaaa caccacttt ccattagag tcaaataat 
        ccaaacatc tctaatctc taatcccct tttcaaacc 
        agactgacc atttaactc aaacggagc cgggaaact 
        atcgactta cgactttac actgctctt aataaacaa 
        tgacataac accatacac aaaacagat actgctttc 
        ctgttgact gttctagga aaaggccat gaagaggcc 
        caacgcgga atccctcta tgaaggatt cgcggcctc 
        ttcacctcc ccaactcca caaacaaat tacaccatc 
        acgctggaa cagaggagc cggaagtaa caactgaac 
        gggggccca aagtcaaca aagaccagc aagataaga 
        acatag