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Gene information
General information
TC005274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450720.1: 48729..52046
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC005274
      iBeetle screen iB_03932

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection

      iBeetle screen iB_05706

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005274 RA
        MAVNSIKIA TANSGGVTR KKAAIENFL FRDNIEILG 
ITEVKAKSF PKFRGFNAI HRRYPAQQG QRETRGVAI 
LIKKGFAFS EHQLPNNLQ HLDCIAVDT YIDSNIITF 
FTYYNSPRQ CLSNELMQY AASLPKAII LGDFNARHT 
DFGDTLTNR NGHILIDAI SNLPLYRIP NSSPTHINH 
WEGYSIVDH ILVTDSLSN RASDSANIG TTITSDHLP 
LVFDLSVSK PRTPTPEYI IIRDSKNTD WEKFRIILE 
KEIVPLDPT TTPEEIERQ VFYISELIQ DAYLQCTPE 
KQIPLHKTP LPPEIVDKI RLKRQVYRE YINTRDPDI 
KTQWNRLNA TIRRDINRH REKLWRETC SGLNYRDGK 
RFWNKLKIL TGQKSNPRH FLKVNNQFI TTDTDKANA 
YAKHLEKIF QIPEDPLFN DSHKKRVET FHSQFIHRT 
QARTCYITQ ADRHKDTQT DDVSTVLEP IQLEEIATK 
IHLLKNNKA PGNDNIKNI TLKHLPHSV LQAIVQIFN 
NCLSTSYFP CLWKSGIAI MIQKPGKPP DDPNSYRPV 
TLLSGIGKL FERIITERL RNFCEGNSL FPPTQFGFR 
AQRSTCDPL TALQTDVAR HLNLGQCVL GVFLDIEKA 
FDKVWHAGL IYKLKQLHF SDQLTQLIH SFLSHRTLK 
VREGNELSN PISINAGVP QGSVLSPLL YLIYCHDIP 
IKSQIATKT ILFADDTAL WTGQKTSFK ATQVLQECL 
DEFQKWASK WRIKPNPTK SSAILFRNG HQCQSWKFQ 
TRNIHLSLW NIQIPLSNE VRYLGISFH KYGNFSSDL 
NATLRKVRQ RAGIISRLR GGLQGCSSE TLHHTYKTF 
IRPVIEFRA VLYVSLNSP EIERLISCE RKILRKIFD 
LYFKFPSER LYEHIGAHS INERLKKLQ SSYVLRTLR 
SQNEIAKQT LRTSWTDPH NKNLSTSQT FPSKPKMKK 
VHLPSALLE ITARENELS ASLENIIEE TPLSIRVK
      CDS sequence
      • >TC005274 RA
        atggcggtt aattccatc aaaatcgcc acggccaac 
tccgggggg gtcacccgg aagaaagcc gcaatagaa 
aatttcctt ttccgggat aatattgaa attctgggt 
atcaccgaa gttaaggcc aaatccttt ccaaaattc 
agaggcttc aatgccatc cacaggaga tacccagct 
caacagggt caacgcgaa actcgagga gtagccatt 
ctaatcaaa aaaggtttt gccttcagc gaacaccaa 
ctccccaac aacttacaa cacttggat tgcatcgcg 
gtcgacact tacattgac tccaacatc attacattc 
tttacctac tacaattcc ccaagacag tgtctaagc 
aacgaatta atgcagtac gccgcatct ctccccaaa 
gccatcatc ctaggcgat tttaatgct agacatact 
gattttggc gacacttta accaatcga aacggccac 
attctcatc gatgcaatc agtaatttg cccctgtac 
agaattccc aactcttcc ccgacccac atcaatcat 
tgggaagga tactctatc gtagatcac atcctcgtc 
acagattcc ttgtccaac cgtgctagc gactctgct 
aacatcgga acaacaatt acgtcagac caccttcca 
ctagtattt gatctatct gtatccaag ccaagaacc 
ccaactcca gagtacatc atcattcga gattccaaa 
aacacagat tgggaaaag tttcgtatc atcctggaa 
aaggaaatt gtacctctt gacccaacc accaccccc 
gaggagatc gagcgccag gtcttctac atatcagaa 
ctaatacaa gacgcatat cttcaatgc actcccgaa 
aaacaaatt ccattgcac aaaaccccg ctcccccct 
gaaattgtc gacaaaatt cggctgaaa cgacaagtt 
tacagagaa tacattaac acccgcgat ccggacatt 
aagacacaa tggaaccga cttaatgcc acaattcga 
cgcgacatt aatcgtcat cgagagaaa ctttggaga 
gaaacatgt tcaggactc aactaccgc gacgggaaa 
cgcttttgg aacaaatta aaaatctta acgggacaa 
aaatcaaac ccacgacac tttttgaaa gttaacaat 
caattcatc acaactgac acagacaaa gcaaacgct 
tacgcaaaa cacttagag aaaattttc caaattcct 
gaagatccg ttgttcaat gactctcat aaaaaacgg 
gtcgagaca ttccactcg cagttcatt caccgcacc 
caagcacgc acctgctac ataacacaa gccgaccgc 
cacaaggac acacaaaca gatgacgtc agcaccgta 
ctcgaaccc attcaatta gaggaaatc gcgacaaaa 
atacactta ctaaaaaac aacaaagca cccggaaat 
gataacatt aaaaacatt acactcaaa caccttcca 
cactctgtc ttgcaggca attgttcaa attttcaac 
aattgtctt tctacgtct tactttcct tgtctctgg 
aaatctgga atcgcaatt atgatccaa aaacccgga 
aaaccccca gacgaccca aactcttat aggccagtc 
acacttctg agtggaatt ggaaaactt tttgagaga 
ataatcacc gaaagactt cgtaatttc tgcgaaggc 
aactcgctc ttccccccc acgcagttt ggattccgt 
gcacaaagg tcaacgtgc gacccactt acggcgctg 
caaacagat gttgcgcgg cacttgaac ctaggtcag 
tgtgtcttg ggagtcttt ctcgacatc gaaaaagct 
ttcgataaa gtttggcac gctggattg atttacaaa 
ttaaaacaa ttacacttt agcgaccaa ttaacacaa 
ctaatacac tcgttttta tcgcacaga acactaaaa 
gttcgagaa ggaaatgaa ctatctaac ccaatcagt 
ataaacgcc ggggttccc caaggttct gttctgtcc 
cctcttctc tacctaatc tactgtcat gatatacca 
atcaaatct caaattgct acaaaaacc attctcttt 
gctgatgac acggccctg tggacagga cagaaaaca 
tctttcaag gccactcaa gtccttcaa gagtgcctg 
gatgaattc caaaagtgg gcttccaaa tggaggata 
aaacctaac ccaaccaag tcttcggcc attctcttc 
cgaaacgga caccaatgc cagtcgtgg aaatttcaa 
acccggaac atccacctt tctctttgg aatatccaa 
attccccta agcaacgag gtccgatat ctaggtatc 
tcattccat aaatatgga aacttttct tctgacttg 
aatgctacc cttcgaaag gtgaggcaa agagcggga 
attatttct aggttacgg ggagggttg caaggctgt 
tcttccgaa accttgcac cacacatat aaaacattc 
atacggccg gtcatcgaa ttccgtgct gtactctat 
gtgtccctc aactctcct gaaatcgaa agactcatc 
tcttgcgaa aggaagatc ttgcgtaaa atcttcgac 
ttatatttc aaattcccg tccgaacga ctgtatgaa 
cacattggc gcacactcg atcaatgag cgtttaaaa 
aagctccag tcgtcttac gtacttcgt acgctccgg 
tcccaaaat gaaatagca aagcaaacg ctccgtaca 
tcttggact gacccccac aataaaaat ctctccact 
tctcaaacc tttccaagc aaacccaaa atgaaaaaa 
gtccacctt ccgagcgct ttgctagaa ataacagcc 
cgtgaaaac gagctgtcg gccagtctg gaaaacatc 
atcgaagaa acaccactt tccattaga gtcaaataa
      mRNA sequence
      • >TC005274 RA
        aagagaagt taaatacaa tttttctgg ggacatgct 
tcatgtctc aattgacaa gttgagtac ccacaaaaa 
tggcggtta attccatca aaatcgcca cggccaact 
ccggggggg tcacccgga agaaagccg caatagaaa 
atttccttt tccgggata atattgaaa ttctgggta 
tcaccgaag ttaaggcca aatcctttc caaaattca 
gaggcttca atgccatcc acaggagat acccagctc 
aacagggtc aacgcgaaa ctcgaggag tagccattc 
taatcaaaa aaggttttg ccttcagcg aacaccaac 
tccccaaca acttacaac acttggatt gcatcgcgg 
tcgacactt acattgact ccaacatca ttacattct 
ttacctact acaattccc caagacagt gtctaagca 
acgaattaa tgcagtacg ccgcatctc tccccaaag 
ccatcatcc taggcgatt ttaatgcta gacatactg 
attttggcg acactttaa ccaatcgaa acggccaca 
ttctcatcg atgcaatca gtaatttgc ccctgtaca 
gaattccca actcttccc cgacccaca tcaatcatt 
gggaaggat actctatcg tagatcaca tcctcgtca 
cagattcct tgtccaacc gtgctagcg actctgcta 
acatcggaa caacaatta cgtcagacc accttccac 
tagtatttg atctatctg tatccaagc caagaaccc 
caactccag agtacatca tcattcgag attccaaaa 
acacagatt gggaaaagt ttcgtatca tcctggaaa 
aggaaattg tacctcttg acccaacca ccacccccg 
aggagatcg agcgccagg tcttctaca tatcagaac 
taatacaag acgcatatc ttcaatgca ctcccgaaa 
aacaaattc cattgcaca aaaccccgc tcccccctg 
aaattgtcg acaaaattc ggctgaaac gacaagttt 
acagagaat acattaaca cccgcgatc cggacatta 
agacacaat ggaaccgac ttaatgcca caattcgac 
gcgacatta atcgtcatc gagagaaac tttggagag 
aaacatgtt caggactca actaccgcg acgggaaac 
gcttttgga acaaattaa aaatcttaa cgggacaaa 
aatcaaacc cacgacact ttttgaaag ttaacaatc 
aattcatca caactgaca cagacaaag caaacgctt 
acgcaaaac acttagaga aaattttcc aaattcctg 
aagatccgt tgttcaatg actctcata aaaaacggg 
tcgagacat tccactcgc agttcattc accgcaccc 
aagcacgca cctgctaca taacacaag ccgaccgcc 
acaaggaca cacaaacag atgacgtca gcaccgtac 
tcgaaccca ttcaattag aggaaatcg cgacaaaaa 
tacacttac taaaaaaca acaaagcac ccggaaatg 
ataacatta aaaacatta cactcaaac accttccac 
actctgtct tgcaggcaa ttgttcaaa ttttcaaca 
attgtcttt ctacgtctt actttcctt gtctctgga 
aatctggaa tcgcaatta tgatccaaa aacccggaa 
aacccccag acgacccaa actcttata ggccagtca 
cacttctga gtggaattg gaaaacttt ttgagagaa 
taatcaccg aaagacttc gtaatttct gcgaaggca 
actcgctct tccccccca cgcagtttg gattccgtg 
cacaaaggt caacgtgcg acccactta cggcgctgc 
aaacagatg ttgcgcggc acttgaacc taggtcagt 
gtgtcttgg gagtctttc tcgacatcg aaaaagctt 
tcgataaag tttggcacg ctggattga tttacaaat 
taaaacaat tacacttta gcgaccaat taacacaac 
taatacact cgtttttat cgcacagaa cactaaaag 
ttcgagaag gaaatgaac tatctaacc caatcagta 
taaacgccg gggttcccc aaggttctg ttctgtccc 
ctcttctct acctaatct actgtcatg atataccaa 
tcaaatctc aaattgcta caaaaacca ttctctttg 
ctgatgaca cggccctgt ggacaggac agaaaacat 
ctttcaagg ccactcaag tccttcaag agtgcctgg 
atgaattcc aaaagtggg cttccaaat ggaggataa 
aacctaacc caaccaagt cttcggcca ttctcttcc 
gaaacggac accaatgcc agtcgtgga aatttcaaa 
cccggaaca tccaccttt ctctttgga atatccaaa 
ttcccctaa gcaacgagg tccgatatc taggtatct 
cattccata aatatggaa acttttctt ctgacttga 
atgctaccc ttcgaaagg tgaggcaaa gagcgggaa 
ttatttcta ggttacggg gagggttgc aaggctgtt 
cttccgaaa ccttgcacc acacatata aaacattca 
tacggccgg tcatcgaat tccgtgctg tactctatg 
tgtccctca actctcctg aaatcgaaa gactcatct 
cttgcgaaa ggaagatct tgcgtaaaa tcttcgact 
tatatttca aattcccgt ccgaacgac tgtatgaac 
acattggcg cacactcga tcaatgagc gtttaaaaa 
agctccagt cgtcttacg tacttcgta cgctccggt 
cccaaaatg aaatagcaa agcaaacgc tccgtacat 
cttggactg acccccaca ataaaaatc tctccactt 
ctcaaacct ttccaagca aacccaaaa tgaaaaaag 
tccaccttc cgagcgctt tgctagaaa taacagccc 
gtgaaaacg agctgtcgg ccagtctgg aaaacatca 
tcgaagaaa caccacttt ccattagag tcaaataat 
ccaaacatc tctaatctc taatcccct tttcaaacc 
agactgacc atttaactc aaacggagc cgggaaact 
atcgactta cgactttac actgctctt aataaacaa 
tgacataac accatacac aaaacagat actgctttc 
ctgttgact gttctagga aaaggccat gaagaggcc 
caacgcgga atccctcta tgaaggatt cgcggcctc 
ttcacctcc ccaactcca caaacaaat tacaccatc 
acgctggaa cagaggagc cggaagtaa caactgaac 
gggggccca aagtcaaca aagaccagc aagataaga 
acatag
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