| General information | |||
| TC005249 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450462.1: 7175..21587 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005249 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03930 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC005249 RA
MSFNEKLVK KEDIREIDV MKEGEVAVK EINDDLGLA FDDTDLKYY TNLFKNVLE RNPTNVELF DLAQSNSEH SRHWFFKGK MVIDGVEHK DSLIDMIVD TQNHSNPNN VIKFSDNSS AVKGYVHRS LRPVTLGTT SELREISTE SHLIFTAET HNFPTGVAP FSGAITGTG GRIRDVQCV GRGGYCIAG TAGYSVGNL NIPGYNLPW EDSKYEYPN NFAPPLEIL VEASNGASD YGNKFGEPL ISGFVRSFG MVDAAGERK EWIKPIMFS GGIGTMEAN MTEKCPPKK GHQIIKIGG PVYRIGVGG GSASSVEVQ GDNKAELDF GAVQRGDAE MEQKLNRVV RACLELGKD NPIVSIHDQ GAGGNGNVL KELVEPVGG IIYANKFEL GDPTINVLE LWGAEYQEN NALLCEKEN LELLKNICK RERCPINIV GEVTGTGRV VLAMDESQK VVPFNLELA HVLGEMPQK VFKLERKTP LLKELTLPD ALSIYSTLE RVLRLPSVS SKRYLTNKV DRCVTGLIA QQQCVGPLH TPLADVAVT AISHFGYEG IASSIGEQP TKGLVNTAA GARMTVAEA LSNLVFALI SDIRDVKCS GNWMWAAKL PGEGAALYD ACKAMCDIM SGLGIAIDG GKDSLSMAA RVGRDIVKA PGTLVVSTY APCPDVRKV VTPDLKAPA IGKVGHLLF VDLSHGYNR LGGAAVAQV FGQLGKESP DVDNVEELK NAFIATQKL IRDGAIQAG HDVSDGGLI VCLLEMSFA GISGMEVQI GHKQGKTIP ILFNEEAGW VLEVLEADL NHCMDVFQK HTVPVYTIG KSIGCGIDS KIRISVNNA CIESTVLPL MRMWEETSY RLEL
- >TC005249 RA
atgagtttt aacgagaag ttggtcaaa aaggaggat attagggag attgatgtg atgaaggag ggcgaagtc gctgttaaa gaaataaat gatgaccta ggtttagct ttcgacgat accgatttg aaatactat acgaatttg tttaaaaat gtgctggaa cgaaatccg accaatgtt gagctgttt gatttggcg caatcgaac agtgaacac agtcggcat tggtttttt aagggaaaa atggttatt gatggagtg gaacataag gactctctt attgacatg attgtcgat acacagaat cactccaat ccgaataat gttatcaag ttcagcgac aatagcagt gctgtgaaa ggttacgtc catcgcagc cttcgcccc gtcacctta ggtaccacc agcgagctt cgcgaaatc agcaccgaa tctcaccta atttttacc gccgaaacg cacaatttt cccaccggt gtggcccca ttcagtggc gccatcact ggaaccggt ggccgaatc cgcgatgtt cagtgcgtc ggacgcggc ggttattgc attgccggt acagccggt tattcagtc ggtaatctc aacataccc ggttataac cttccatgg gaagactcc aagtacgaa tatccgaac aatttcgcg cccccgctt gaaattcta gtcgaagct agtaatgga gcttccgat tacggaaat aagtttgga gaaccgttg attagcggt tttgtacgg tcattcgga atggtggat gcagcagga gagagaaaa gagtggatc aaaccgatt atgtttagt ggaggaatc gggacaatg gaagccaac atgacggag aaatgtccc ccaaaaaag gggcaccag attattaag attggagga ccagtttat agaattgga gtaggagga ggatccgct agttcagtg gaggtccaa ggggacaat aaggccgaa cttgacttt ggcgcagtg cagcgaggt gacgccgaa atggaacag aaactgaat cgagtggtg cgtgcgtgt ctcgaattg ggaaaagac aaccccatt gtcagtatc cacgatcaa ggcgctggt ggcaacgga aacgtactt aaggaactc gttgaacca gttggtggt ataatctac gccaacaaa tttgaattg ggtgacccc actatcaac gtccttgaa ctatggggc gccgaatac caagaaaac aacgccctc ttgtgcgaa aaagaaaat ttagaactg ttgaaaaac atatgcaaa cgcgaacga tgtccaata aacattgtt ggtgaggtg accggaacc ggaagagtc gttctggcc atggacgaa tcgcagaaa gtcgttccg ttcaattta gaattggcg cacgtatta ggggaaatg ccacagaaa gtgtttaaa ctggaacgc aaaacgccc cttcttaaa gaactgaca cttcccgac gctctctcg atttacagc acattggag cgcgtcctg cgtttaccg tcagtgtcc agcaaacgc taccttacc aataaagtt gaccgttgc gttaccggt ctcatagct caacaacaa tgcgtcggt ccgcttcac acccctctt gccgatgtt gccgtcact gcaatttct catttcggt tacgaaggt atagcgtca tctatcggt gaacaacca accaaagga cttgtgaat actgcagcc ggtgctcgt atgacagtc gccgaagcc ctctcgaat cttgttttc gcactaatt agtgacatt cgtgacgtc aaatgcagt ggcaactgg atgtgggcg gcgaaattg ccaggagaa ggcgccgcc ctctacgat gcttgtaag gccatgtgc gatatcatg tcaggcctt ggaattgcg atagatggc ggtaaagac tctctaagt atggcggcg agagttggt agagacatt gttaaggca cccggaact ttggtcgtt tcgacttat gctccatgt ccggatgtc cggaaagta gtaactccg gatttgaaa gcaccagcg atcggaaaa gttgggcat ttgttgttt gttgatttg tcgcatggg tataataga ctaggtggc gctgcagtc gcacaagtt tttggacag ttgggcaag gaaagtccc gatgtggat aatgtagaa gagttgaaa aatgcattt atcgcaacg cagaaattg ataagagat ggcgctatt caagccgga catgacgtc agcgacggt ggcttgatt gtttgttta ttggaaatg tctttcgct ggaattagc ggaatggaa gtccaaatc ggtcacaaa caaggaaag acaataccg attttgttt aatgaggaa gcgggctgg gttttagaa gtgttggag gctgattta aatcattgt atggatgtt ttccagaaa catacagta ccagtctac acaatcggc aaaagtatc ggttgcgga atcgattcg aaaatcaga atttcagtc aacaacgca tgtatcgaa agcacagtg ttgccactc atgcgcatg tgggaggag actagctac agattggag ctttaa
- >TC005249 RA
agtcattac aatgtgaaa cccaaatac gagttttat ttcagtccc acctacaaa aatcacata caacacatg acttacgtc aaaaacctg ctgcaaaat attacagta agattctta taattttgg tcgaatgtc gtaaaacgt tgtcttttt cactcttta agaaagcag gtgggagca agtaacatc actcggctg acggatatc aaactctaa tagtgattt tcccgtaat tatgttgac tgaagagtc catctcgcg tttttacca aaaacctgg ttgtacgag tgcgaaaaa agccgaact ccttttgaa actccagga gaagaatga gaaattgtt cgatttgga aaccgagct ttgctacta cgttgaaac aacagcccc cttgttgcc caatgacaa gactttgat caaatggct cttgagcga ccccttcca gcccgacaa tctgtccga aacgcctca cttgtcgga aggggactc aagtgtgat tgtggaagt ggggcccag atttagttt ctccacttc gaatcgacg aatgctgtt tccatttca cggaatgtg gggctgtcg cacgtggtg cgagtggaa gtctcgaga cggtataaa cttgtcttc catgggact gtgtccaaa gttgacgaa attgcgtcg gctttgtac gatcgcatg actgaatgt cgctacacg cctgagagt tttcccaag atgagtttt aacgagaag ttggtcaaa aaggaggat attagggag attgatgtg atgaaggag ggcgaagtc gctgttaaa gaaataaat gatgaccta ggtttagct ttcgacgat accgatttg aaatactat acgaatttg tttaaaaat gtgctggaa cgaaatccg accaatgtt gagctgttt gatttggcg caatcgaac agtgaacac agtcggcat tggtttttt aagggaaaa atggttatt gatggagtg gaacataag gactctctt attgacatg attgtcgat acacagaat cactccaat ccgaataat gttatcaag ttcagcgac aatagcagt gctgtgaaa ggttacgtc catcgcagc cttcgcccc gtcacctta ggtaccacc agcgagctt cgcgaaatc agcaccgaa tctcaccta atttttacc gccgaaacg cacaatttt cccaccggt gtggcccca ttcagtggc gccatcact ggaaccggt ggccgaatc cgcgatgtt cagtgcgtc ggacgcggc ggttattgc attgccggt acagccggt tattcagtc ggtaatctc aacataccc ggttataac cttccatgg gaagactcc aagtacgaa tatccgaac aatttcgcg cccccgctt gaaattcta gtcgaagct agtaatgga gcttccgat tacggaaat aagtttgga gaaccgttg attagcggt tttgtacgg tcattcgga atggtggat gcagcagga gagagaaaa gagtggatc aaaccgatt atgtttagt ggaggaatc gggacaatg gaagccaac atgacggag aaatgtccc ccaaaaaag gggcaccag attattaag attggagga ccagtttat agaattgga gtaggagga ggatccgct agttcagtg gaggtccaa ggggacaat aaggccgaa cttgacttt ggcgcagtg cagcgaggt gacgccgaa atggaacag aaactgaat cgagtggtg cgtgcgtgt ctcgaattg ggaaaagac aaccccatt gtcagtatc cacgatcaa ggcgctggt ggcaacgga aacgtactt aaggaactc gttgaacca gttggtggt ataatctac gccaacaaa tttgaattg ggtgacccc actatcaac gtccttgaa ctatggggc gccgaatac caagaaaac aacgccctc ttgtgcgaa aaagaaaat ttagaactg ttgaaaaac atatgcaaa cgcgaacga tgtccaata aacattgtt ggtgaggtg accggaacc ggaagagtc gttctggcc atggacgaa tcgcagaaa gtcgttccg ttcaattta gaattggcg cacgtatta ggggaaatg ccacagaaa gtgtttaaa ctggaacgc aaaacgccc cttcttaaa gaactgaca cttcccgac gctctctcg atttacagc acattggag cgcgtcctg cgtttaccg tcagtgtcc agcaaacgc taccttacc aataaagtt gaccgttgc gttaccggt ctcatagct caacaacaa tgcgtcggt ccgcttcac acccctctt gccgatgtt gccgtcact gcaatttct catttcggt tacgaaggt atagcgtca tctatcggt gaacaacca accaaagga cttgtgaat actgcagcc ggtgctcgt atgacagtc gccgaagcc ctctcgaat cttgttttc gcactaatt agtgacatt cgtgacgtc aaatgcagt ggcaactgg atgtgggcg gcgaaattg ccaggagaa ggcgccgcc ctctacgat gcttgtaag gccatgtgc gatatcatg tcaggcctt ggaattgcg atagatggc ggtaaagac tctctaagt atggcggcg agagttggt agagacatt gttaaggca cccggaact ttggtcgtt tcgacttat gctccatgt ccggatgtc cggaaagta gtaactccg gatttgaaa gcaccagcg atcggaaaa gttgggcat ttgttgttt gttgatttg tcgcatggg tataataga ctaggtggc gctgcagtc gcacaagtt tttggacag ttgggcaag gaaagtccc gatgtggat aatgtagaa gagttgaaa aatgcattt atcgcaacg cagaaattg ataagagat ggcgctatt caagccgga catgacgtc agcgacggt ggcttgatt gtttgttta ttggaaatg tctttcgct ggaattagc ggaatggaa gtccaaatc ggtcacaaa caaggaaag acaataccg attttgttt aatgaggaa gcgggctgg gttttagaa gtgttggag gctgattta aatcattgt atggatgtt ttccagaaa catacagta ccagtctac acaatcggc aaaagtatc ggttgcgga atcgattcg aaaatcaga atttcagtc aacaacgca tgtatcgaa agcacagtg ttgccactc atgcgcatg tgggaggag actagctac agattggag ctttaacaa acgaccaaa tcgtgtgcc gactctgaa tacaactcg ctaacatca cgaaaacgc cctgtgtat aaactaact tttgacccg gacgcaaaa tctgtcctt aaaaagtcg acacttgtg aaagtcgct gtcttaaga gaggaaggt acaaatggc gatcgagag atggcagcg gctctagtt cgagtggga ttcaaagtt tgggatgta acgatgcaa gatttactt agcggaaag gtggatttg gcccaattc cgcgggatt gtattcccc ggaggtttt agttatgcc ggtatggca atttatagc gacaaaatt ccctagatg aaatttaat aattttttt agatgtttt gggctcggc taaaggctg ggctggaag tatcctctt caataaaac agttaagga gcaatttga ccggttcta cgcccggtc tgatacttt cagtcttgg tgtgtgcaa cggttgcca acttatggc catgatagg gtgggttgg tgaactcag tgctgacaa cactccgaa cattgtcct taaacacaa cgtttccga acgattcga atgtcggtg gagtacaat ccgaatcga aaaatcccc cgccattat gttgaaaga tatgcacca gtctgtctt tggtgtgtg gtccgcaca cggtgaagg ccagtttac tttcaaaaa ttcgtcggt ttatgatga tttggttaa gaatcattg cgtctgttt gcgattcac agacgacga ggggaatcc cactgaaac gtatccaat gaaccccaa cggcagcat tgccggtac tgcagggat ctgctcaaa aggtggccg ccatttggc catgatgcc acatcctga acgttgtga ccaatcgta tttgtggcc atacatgcc gccaagttg gcaacactt ccagaaaag cccctggga aaaaatgtt ccgaaacgc ttacgaatg gtgttgcag ttaaccttt ttcctgcgg gagacttaa gcagaggac cgtctccat accgtgtta cgcagcaaa ttagacctt acgtcttgc agtgataaa agtaatgtt ttttatgtt gaaattaat aaaaaatct tgttatttt gtttatata cgtctattt attacttga aaaaatatt aatataaaa tgtgcacaa at
