Status
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Gene information
General information
TC005249 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450462.1: 7175..21587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005249
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005249 RA
      MSFNEKLVK KEDIREIDV MKEGEVAVK EINDDLGLA 
      FDDTDLKYY TNLFKNVLE RNPTNVELF DLAQSNSEH 
      SRHWFFKGK MVIDGVEHK DSLIDMIVD TQNHSNPNN 
      VIKFSDNSS AVKGYVHRS LRPVTLGTT SELREISTE 
      SHLIFTAET HNFPTGVAP FSGAITGTG GRIRDVQCV 
      GRGGYCIAG TAGYSVGNL NIPGYNLPW EDSKYEYPN 
      NFAPPLEIL VEASNGASD YGNKFGEPL ISGFVRSFG 
      MVDAAGERK EWIKPIMFS GGIGTMEAN MTEKCPPKK 
      GHQIIKIGG PVYRIGVGG GSASSVEVQ GDNKAELDF 
      GAVQRGDAE MEQKLNRVV RACLELGKD NPIVSIHDQ 
      GAGGNGNVL KELVEPVGG IIYANKFEL GDPTINVLE 
      LWGAEYQEN NALLCEKEN LELLKNICK RERCPINIV 
      GEVTGTGRV VLAMDESQK VVPFNLELA HVLGEMPQK 
      VFKLERKTP LLKELTLPD ALSIYSTLE RVLRLPSVS 
      SKRYLTNKV DRCVTGLIA QQQCVGPLH TPLADVAVT 
      AISHFGYEG IASSIGEQP TKGLVNTAA GARMTVAEA 
      LSNLVFALI SDIRDVKCS GNWMWAAKL PGEGAALYD 
      ACKAMCDIM SGLGIAIDG GKDSLSMAA RVGRDIVKA 
      PGTLVVSTY APCPDVRKV VTPDLKAPA IGKVGHLLF 
      VDLSHGYNR LGGAAVAQV FGQLGKESP DVDNVEELK 
      NAFIATQKL IRDGAIQAG HDVSDGGLI VCLLEMSFA 
      GISGMEVQI GHKQGKTIP ILFNEEAGW VLEVLEADL 
      NHCMDVFQK HTVPVYTIG KSIGCGIDS KIRISVNNA 
      CIESTVLPL MRMWEETSY RLEL
    CDS sequence
    • >TC005249 RA
      atgagtttt aacgagaag ttggtcaaa aaggaggat 
      attagggag attgatgtg atgaaggag ggcgaagtc 
      gctgttaaa gaaataaat gatgaccta ggtttagct 
      ttcgacgat accgatttg aaatactat acgaatttg 
      tttaaaaat gtgctggaa cgaaatccg accaatgtt 
      gagctgttt gatttggcg caatcgaac agtgaacac 
      agtcggcat tggtttttt aagggaaaa atggttatt 
      gatggagtg gaacataag gactctctt attgacatg 
      attgtcgat acacagaat cactccaat ccgaataat 
      gttatcaag ttcagcgac aatagcagt gctgtgaaa 
      ggttacgtc catcgcagc cttcgcccc gtcacctta 
      ggtaccacc agcgagctt cgcgaaatc agcaccgaa 
      tctcaccta atttttacc gccgaaacg cacaatttt 
      cccaccggt gtggcccca ttcagtggc gccatcact 
      ggaaccggt ggccgaatc cgcgatgtt cagtgcgtc 
      ggacgcggc ggttattgc attgccggt acagccggt 
      tattcagtc ggtaatctc aacataccc ggttataac 
      cttccatgg gaagactcc aagtacgaa tatccgaac 
      aatttcgcg cccccgctt gaaattcta gtcgaagct 
      agtaatgga gcttccgat tacggaaat aagtttgga 
      gaaccgttg attagcggt tttgtacgg tcattcgga 
      atggtggat gcagcagga gagagaaaa gagtggatc 
      aaaccgatt atgtttagt ggaggaatc gggacaatg 
      gaagccaac atgacggag aaatgtccc ccaaaaaag 
      gggcaccag attattaag attggagga ccagtttat 
      agaattgga gtaggagga ggatccgct agttcagtg 
      gaggtccaa ggggacaat aaggccgaa cttgacttt 
      ggcgcagtg cagcgaggt gacgccgaa atggaacag 
      aaactgaat cgagtggtg cgtgcgtgt ctcgaattg 
      ggaaaagac aaccccatt gtcagtatc cacgatcaa 
      ggcgctggt ggcaacgga aacgtactt aaggaactc 
      gttgaacca gttggtggt ataatctac gccaacaaa 
      tttgaattg ggtgacccc actatcaac gtccttgaa 
      ctatggggc gccgaatac caagaaaac aacgccctc 
      ttgtgcgaa aaagaaaat ttagaactg ttgaaaaac 
      atatgcaaa cgcgaacga tgtccaata aacattgtt 
      ggtgaggtg accggaacc ggaagagtc gttctggcc 
      atggacgaa tcgcagaaa gtcgttccg ttcaattta 
      gaattggcg cacgtatta ggggaaatg ccacagaaa 
      gtgtttaaa ctggaacgc aaaacgccc cttcttaaa 
      gaactgaca cttcccgac gctctctcg atttacagc 
      acattggag cgcgtcctg cgtttaccg tcagtgtcc 
      agcaaacgc taccttacc aataaagtt gaccgttgc 
      gttaccggt ctcatagct caacaacaa tgcgtcggt 
      ccgcttcac acccctctt gccgatgtt gccgtcact 
      gcaatttct catttcggt tacgaaggt atagcgtca 
      tctatcggt gaacaacca accaaagga cttgtgaat 
      actgcagcc ggtgctcgt atgacagtc gccgaagcc 
      ctctcgaat cttgttttc gcactaatt agtgacatt 
      cgtgacgtc aaatgcagt ggcaactgg atgtgggcg 
      gcgaaattg ccaggagaa ggcgccgcc ctctacgat 
      gcttgtaag gccatgtgc gatatcatg tcaggcctt 
      ggaattgcg atagatggc ggtaaagac tctctaagt 
      atggcggcg agagttggt agagacatt gttaaggca 
      cccggaact ttggtcgtt tcgacttat gctccatgt 
      ccggatgtc cggaaagta gtaactccg gatttgaaa 
      gcaccagcg atcggaaaa gttgggcat ttgttgttt 
      gttgatttg tcgcatggg tataataga ctaggtggc 
      gctgcagtc gcacaagtt tttggacag ttgggcaag 
      gaaagtccc gatgtggat aatgtagaa gagttgaaa 
      aatgcattt atcgcaacg cagaaattg ataagagat 
      ggcgctatt caagccgga catgacgtc agcgacggt 
      ggcttgatt gtttgttta ttggaaatg tctttcgct 
      ggaattagc ggaatggaa gtccaaatc ggtcacaaa 
      caaggaaag acaataccg attttgttt aatgaggaa 
      gcgggctgg gttttagaa gtgttggag gctgattta 
      aatcattgt atggatgtt ttccagaaa catacagta 
      ccagtctac acaatcggc aaaagtatc ggttgcgga 
      atcgattcg aaaatcaga atttcagtc aacaacgca 
      tgtatcgaa agcacagtg ttgccactc atgcgcatg 
      tgggaggag actagctac agattggag ctttaa
    mRNA sequence
    • >TC005249 RA
      agtcattac aatgtgaaa cccaaatac gagttttat 
      ttcagtccc acctacaaa aatcacata caacacatg 
      acttacgtc aaaaacctg ctgcaaaat attacagta 
      agattctta taattttgg tcgaatgtc gtaaaacgt 
      tgtcttttt cactcttta agaaagcag gtgggagca 
      agtaacatc actcggctg acggatatc aaactctaa 
      tagtgattt tcccgtaat tatgttgac tgaagagtc 
      catctcgcg tttttacca aaaacctgg ttgtacgag 
      tgcgaaaaa agccgaact ccttttgaa actccagga 
      gaagaatga gaaattgtt cgatttgga aaccgagct 
      ttgctacta cgttgaaac aacagcccc cttgttgcc 
      caatgacaa gactttgat caaatggct cttgagcga 
      ccccttcca gcccgacaa tctgtccga aacgcctca 
      cttgtcgga aggggactc aagtgtgat tgtggaagt 
      ggggcccag atttagttt ctccacttc gaatcgacg 
      aatgctgtt tccatttca cggaatgtg gggctgtcg 
      cacgtggtg cgagtggaa gtctcgaga cggtataaa 
      cttgtcttc catgggact gtgtccaaa gttgacgaa 
      attgcgtcg gctttgtac gatcgcatg actgaatgt 
      cgctacacg cctgagagt tttcccaag atgagtttt 
      aacgagaag ttggtcaaa aaggaggat attagggag 
      attgatgtg atgaaggag ggcgaagtc gctgttaaa 
      gaaataaat gatgaccta ggtttagct ttcgacgat 
      accgatttg aaatactat acgaatttg tttaaaaat 
      gtgctggaa cgaaatccg accaatgtt gagctgttt 
      gatttggcg caatcgaac agtgaacac agtcggcat 
      tggtttttt aagggaaaa atggttatt gatggagtg 
      gaacataag gactctctt attgacatg attgtcgat 
      acacagaat cactccaat ccgaataat gttatcaag 
      ttcagcgac aatagcagt gctgtgaaa ggttacgtc 
      catcgcagc cttcgcccc gtcacctta ggtaccacc 
      agcgagctt cgcgaaatc agcaccgaa tctcaccta 
      atttttacc gccgaaacg cacaatttt cccaccggt 
      gtggcccca ttcagtggc gccatcact ggaaccggt 
      ggccgaatc cgcgatgtt cagtgcgtc ggacgcggc 
      ggttattgc attgccggt acagccggt tattcagtc 
      ggtaatctc aacataccc ggttataac cttccatgg 
      gaagactcc aagtacgaa tatccgaac aatttcgcg 
      cccccgctt gaaattcta gtcgaagct agtaatgga 
      gcttccgat tacggaaat aagtttgga gaaccgttg 
      attagcggt tttgtacgg tcattcgga atggtggat 
      gcagcagga gagagaaaa gagtggatc aaaccgatt 
      atgtttagt ggaggaatc gggacaatg gaagccaac 
      atgacggag aaatgtccc ccaaaaaag gggcaccag 
      attattaag attggagga ccagtttat agaattgga 
      gtaggagga ggatccgct agttcagtg gaggtccaa 
      ggggacaat aaggccgaa cttgacttt ggcgcagtg 
      cagcgaggt gacgccgaa atggaacag aaactgaat 
      cgagtggtg cgtgcgtgt ctcgaattg ggaaaagac 
      aaccccatt gtcagtatc cacgatcaa ggcgctggt 
      ggcaacgga aacgtactt aaggaactc gttgaacca 
      gttggtggt ataatctac gccaacaaa tttgaattg 
      ggtgacccc actatcaac gtccttgaa ctatggggc 
      gccgaatac caagaaaac aacgccctc ttgtgcgaa 
      aaagaaaat ttagaactg ttgaaaaac atatgcaaa 
      cgcgaacga tgtccaata aacattgtt ggtgaggtg 
      accggaacc ggaagagtc gttctggcc atggacgaa 
      tcgcagaaa gtcgttccg ttcaattta gaattggcg 
      cacgtatta ggggaaatg ccacagaaa gtgtttaaa 
      ctggaacgc aaaacgccc cttcttaaa gaactgaca 
      cttcccgac gctctctcg atttacagc acattggag 
      cgcgtcctg cgtttaccg tcagtgtcc agcaaacgc 
      taccttacc aataaagtt gaccgttgc gttaccggt 
      ctcatagct caacaacaa tgcgtcggt ccgcttcac 
      acccctctt gccgatgtt gccgtcact gcaatttct 
      catttcggt tacgaaggt atagcgtca tctatcggt 
      gaacaacca accaaagga cttgtgaat actgcagcc 
      ggtgctcgt atgacagtc gccgaagcc ctctcgaat 
      cttgttttc gcactaatt agtgacatt cgtgacgtc 
      aaatgcagt ggcaactgg atgtgggcg gcgaaattg 
      ccaggagaa ggcgccgcc ctctacgat gcttgtaag 
      gccatgtgc gatatcatg tcaggcctt ggaattgcg 
      atagatggc ggtaaagac tctctaagt atggcggcg 
      agagttggt agagacatt gttaaggca cccggaact 
      ttggtcgtt tcgacttat gctccatgt ccggatgtc 
      cggaaagta gtaactccg gatttgaaa gcaccagcg 
      atcggaaaa gttgggcat ttgttgttt gttgatttg 
      tcgcatggg tataataga ctaggtggc gctgcagtc 
      gcacaagtt tttggacag ttgggcaag gaaagtccc 
      gatgtggat aatgtagaa gagttgaaa aatgcattt 
      atcgcaacg cagaaattg ataagagat ggcgctatt 
      caagccgga catgacgtc agcgacggt ggcttgatt 
      gtttgttta ttggaaatg tctttcgct ggaattagc 
      ggaatggaa gtccaaatc ggtcacaaa caaggaaag 
      acaataccg attttgttt aatgaggaa gcgggctgg 
      gttttagaa gtgttggag gctgattta aatcattgt 
      atggatgtt ttccagaaa catacagta ccagtctac 
      acaatcggc aaaagtatc ggttgcgga atcgattcg 
      aaaatcaga atttcagtc aacaacgca tgtatcgaa 
      agcacagtg ttgccactc atgcgcatg tgggaggag 
      actagctac agattggag ctttaacaa acgaccaaa 
      tcgtgtgcc gactctgaa tacaactcg ctaacatca 
      cgaaaacgc cctgtgtat aaactaact tttgacccg 
      gacgcaaaa tctgtcctt aaaaagtcg acacttgtg 
      aaagtcgct gtcttaaga gaggaaggt acaaatggc 
      gatcgagag atggcagcg gctctagtt cgagtggga 
      ttcaaagtt tgggatgta acgatgcaa gatttactt 
      agcggaaag gtggatttg gcccaattc cgcgggatt 
      gtattcccc ggaggtttt agttatgcc ggtatggca 
      atttatagc gacaaaatt ccctagatg aaatttaat 
      aattttttt agatgtttt gggctcggc taaaggctg 
      ggctggaag tatcctctt caataaaac agttaagga 
      gcaatttga ccggttcta cgcccggtc tgatacttt 
      cagtcttgg tgtgtgcaa cggttgcca acttatggc 
      catgatagg gtgggttgg tgaactcag tgctgacaa 
      cactccgaa cattgtcct taaacacaa cgtttccga 
      acgattcga atgtcggtg gagtacaat ccgaatcga 
      aaaatcccc cgccattat gttgaaaga tatgcacca 
      gtctgtctt tggtgtgtg gtccgcaca cggtgaagg 
      ccagtttac tttcaaaaa ttcgtcggt ttatgatga 
      tttggttaa gaatcattg cgtctgttt gcgattcac 
      agacgacga ggggaatcc cactgaaac gtatccaat 
      gaaccccaa cggcagcat tgccggtac tgcagggat 
      ctgctcaaa aggtggccg ccatttggc catgatgcc 
      acatcctga acgttgtga ccaatcgta tttgtggcc 
      atacatgcc gccaagttg gcaacactt ccagaaaag 
      cccctggga aaaaatgtt ccgaaacgc ttacgaatg 
      gtgttgcag ttaaccttt ttcctgcgg gagacttaa 
      gcagaggac cgtctccat accgtgtta cgcagcaaa 
      ttagacctt acgtcttgc agtgataaa agtaatgtt 
      ttttatgtt gaaattaat aaaaaatct tgttatttt 
      gtttatata cgtctattt attacttga aaaaatatt 
      aatataaaa tgtgcacaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY