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Gene information
General information
TC032006
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005237,TC005236
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid5: 600986..664004
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032006
    iBeetle screen iB_00819
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature dorsal partiallyshortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    iBeetle screen iB_03928
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032006 RA
      MNFPLFLLV FCLAKDGDA GGSIPGIEL SMPPSHIAL 
      SGDLHIQIT SSSFPPLML QLSRLEGNI AQPLTTFPV 
      LPEARALSL NTTIVKIPC GYFSKGGQY YILIKKQPI 
      GGSNSTLVG DDDSVITRS LDVRWPMPQ LSVTPEHIQ 
      TYPETPVMA ILEFPEVVC PPVTDSPIS AIPEFWLEL 
      HYCGHSLLT CDNDDNRQN NSNVQVLDQ VRGFGGRRV 
      FTLRCELFG LAGFYALFL RPTATTQLL PHTAAYVKA 
      DWSEQFVFN VHARSILPC DGHSGIKVL FQYPSCILA 
      SGDRVRVFA RLRANVASL APPTSLEYV AEKRVIRGQ 
      HSLHFDCDL FTERYVEYC FVYVSQAIN EAVADVRMD 
      CVPTLPVTE NESGGWGPW SPWTPCSST CIGGTRSRY 
      RFCDSPPPR YGAKFCELC YINYNMAKG KKKGKKKKK 
      APKIDPNAL TEVDKTFYE LTITDLNRK LARLRSLTT 
      ELEEKNEQL QTDLSKLDE DRNDIIIYL KRMLQERSD 
      EIAELQERL RALEETRQD ETEQYEAKI TDLENEYKQ 
      MHEQLTSEI KLLEGKLNS LEEFRIQRD ELMKKFEDQ 
      EIAMEEQEK RHKNELYDI ERKFIIGKD KLKKDMEAR 
      LLQLSSEFQ EATELRIAA TTHRVIREN IAINNELDA 
      MLITHQKLH SDNAKMKDR DRTLRQETE LHEAEKKKA 
      LNKVRVQLK LIDRLTTEH EDMKQKLTK YETTEQEMH 
      QTSEELQKT KQDVLCLNH KIKLLEQNI HALKCRESS 
      LKTELSNLD FDNDRLTTV LAQAVLSIK EVLELQSRM 
      SDEALKASK RENLLTQLF VLLSEAETK KGRKASLDT 
      IGSLSATYE RGDLGFVPK PLTRRSTLS TLRDIQAQT 
      GPSFDEFLA IGKEEDLEQ QETSLVSSE EESKGSEGT 
      LVEEESDVF FDEEEEAAD ESMLEYDDV ELLDEEDEE 
      IKAAREVAA TKSANELIV ERALSSAAI AKKLSGIGS 
      KEKSRSRIS FKASKEKGV GQSSKEEVA EKKSQEKEL 
      GKASREGMS TDKLKRVSI GETTEMVIP GETAAPLPK 
      NGDKSAETS TSEPST
    • >TC032006 RB
      MKKIALCRA LMNFPLFLL VFCLAKDGD AGGSIPGIE 
      LSMPPSHIA LSGDLHIQI TSSSFPPLM LQLSRLEGN 
      IAQPLTTFP VLPEARALS LNTTIVKIP CGYFSKGGQ 
      YYILIKKQP IGGSNSTLV GDDDSVITR SLDVRWPMP 
      QLSVTPEHI QTYPETPVM AILEFPEVV CPPVTDSPI 
      SAIPEFWLE LHYCGHSLL TCDNDDNRQ NNSNVQVLD 
      QVRGFGGRR VFTLRCELF GLAGFYALF LRPTATTQL 
      LPHTAAYVK ADWSEQFVF NVHARSILP CDGHSGIKV 
      LFQYPSCIL ASGDRVRVF ARLRANVAS LAPPTSLEY 
      VAEKRVIRG QHSLHFDCD LFTERYVEY CFVYVSQAI 
      NEAVADVRM DCVPTLPVT ENESGGWGP WSPWTPCSS 
      TCIGGTRSR YRFCDSPPP RYGAKFCEL CYINYNMAK 
      GKKKGKKKK KAPKIDPNA LTEVDKTFY ELTITDLNR 
      KLARLRSLT TELEEKNEQ LQTDLSKLD EDRNDIIIY 
      LKRMLQERS DEIAELQER LRALEETRQ DETEQYEAK 
      ITDLENEYK QMHEQLTSE IKLLEGKLN SLEEFRIQR 
      DELMKKFED QEIAMEEQE KRHKNELYD IERKFIIGK 
      DKLKKDMEA RLLQLSSEF QEATELRIA ATTHRVIRE 
      NIAINNELD AMLITHQKL HSDNAKMKD RDRTLRQET 
      ELHEAEKKK ALNKVRVQL KLIDRLTTE HEDMKQKLT 
      KYETTEQEM HQTSEELQK TKQDVLCLN HKIKLLEQN 
      IHALKCRES SLKTELSNL DFDNDRLTT VLAQAVLSI 
      KEVLELQSR MSDEALKAS KRENLLTQL FVLLSEAET 
      KKGRKASLD TIGSLSATY ERGDLGFVP KPLTRRSTL 
      STLRDIQAQ TGPSFDEFL AIGKEEDLE QQETSLVSS 
      EEESKGSEG TLVEEESDV FFDEEEEAA DESMLEYDD 
      VELLDEEDE EIKAAREVA ATKSANELI VERALSSAA 
      IAKKLSGIG SKEKSRSRI SFKASKEKG VGQSSKEEV 
      AEKKSQEKE LGKASREGM STDKLKRVS IGETTEMVI 
      PGETAAPLP KNGDKSAET STSEPST
    CDS sequence
    • >TC032006 RA
      atgaatttt ccgttgttc ctgttggtt ttttgtcta 
      gcaaaggac ggagatgca ggaggaagc atcccagga 
      atagaatta tcgatgccg ccttcccac atcgccttg 
      agcggcgac ctccacatc caaattact tcatcgtct 
      tttccacca ctaatgctg caattatct cgactcgaa 
      ggcaacatc gcccagccc ctaaccact tttcccgtt 
      ctacccgaa gcgcgcgct ctttccctc aatacgaca 
      attgtgaaa ataccttgc ggatacttt tccaaaggc 
      ggccagtat tacatactt attaagaag caaccaatt 
      ggtggcagc aattcaact cttgttgga gacgacgat 
      tcggttatc acacgaagt ttggacgta cggtggcca 
      atgcctcaa ctatcggtg acaccggaa catatacaa 
      acttatccg gagactccg gtgatggcg attctcgag 
      tttccggaa gtggtgtgt cctccggtg acggacagt 
      ccgattagt gcgataccg gagttttgg ttagagtta 
      cattattgc ggccattcg ttgcttacc tgtgataat 
      gacgataac agacagaat aattcaaat gttcaggta 
      ttggatcaa gtgcgggga tttggagga aggcgggtg 
      tttacactt aggtgtgaa ctctttggt ttagctgga 
      ttttatgca ctttttcta cgaccgact gctactaca 
      caattactg cctcatact gctgcctac gtcaaggcc 
      gattggagc gagcagttc gtgtttaat gtccatgcc 
      cgaagcatt ctgccttgt gacggccac agcggcatc 
      aaagtcctg ttccaatac ccgtcgtgt attctggca 
      tcaggggac cgagtccga gttttcgct cggttgcga 
      gccaatgtt gcatcactg gcaccccca acatccctc 
      gaatatgtg gcagaaaag cgagttatc agaggacag 
      cacagtttg cactttgat tgtgattta ttcaccgaa 
      cgctatgtc gaatattgc ttcgtgtat gtgagccaa 
      gcaatcaac gaagctgtc gctgacgtg aggatggat 
      tgcgtgccc acccttcct gtcacagag aatgaaagt 
      ggtggttgg ggtccttgg agcccttgg acgccttgt 
      tcaagcaca tgcattggg ggtacaaga agtcgttat 
      cgtttttgt gactcccca cctcctcgt tacggcgcc 
      aagttttgc gagttgtgc tacatcaac tacaatatg 
      gcgaaaggt aaaaagaaa ggcaagaag aagaagaaa 
      gcgcctaaa atcgaccca aacgcctta accgaagtc 
      gataaaaca ttctatgag ttaacaata accgattta 
      aaccgaaaa ttggcccgc ttacgcagt ttaaccact 
      gaactcgag gagaaaaac gaacaatta caaaccgat 
      ttgtccaaa ctcgatgaa gatcgcaac gacattatc 
      atctatctt aagcggatg cttcaagaa cgttctgat 
      gaaatagcc gaacttcag gaacgtttg cgtgctttg 
      gaggaaaca cgtcaagat gaaacggaa caatatgaa 
      gcgaaaatt accgatttg gaaaacgaa tacaagcaa 
      atgcacgaa cagttaacc agcgagatt aaattactt 
      gaaggtaaa ttaaattcg ttggaagaa tttcgcatt 
      caaagggat gagttgatg aaaaaattc gaagaccag 
      gaaatcgca atggaagag caggagaaa cggcacaag 
      aacgaattg tatgacatt gaacgaaaa tttattatt 
      gggaaagat aaactgaag aaagatatg gaagcaagg 
      ttactccaa ctatcgtcc gaatttcag gaagcaact 
      gaactacga attgctgcc accactcac agagtgatt 
      cgagaaaac attgcaatt aataacgaa ctagatgca 
      atgttgatc actcaccaa aaactacac tccgataat 
      gcgaaaatg aaagacagg gatcgaact ttgcgccaa 
      gaaactgaa cttcatgaa gctgaaaag aagaaagct 
      ttaaataag gtcagggtt cagttgaaa ctaattgat 
      cgattaacc actgaacac gaagatatg aaacaaaaa 
      ttaaccaaa tatgaaaca acagaacaa gagatgcat 
      caaacttct gaagaactc cagaaaacg aaacaggac 
      gtcctttgc ctaaaccac aaaattaaa ctcttggaa 
      caaaacatt cacgcattg aaatgtcga gaaagctcg 
      ctcaagacg gagttaagt aaccttgat tttgataac 
      gatcgtctg acaacagtt cttgcccaa gctgtttta 
      tcaattaag gaagttttg gaattacag tcacgaatg 
      tcagatgaa gcactgaaa gcatcaaaa cgtgaaaat 
      ctcctaacc caacttttc gttttatta agtgaagct 
      gagacgaag aaaggtcgt aaagcttct ttggacact 
      attggttcc ttgtctgca acttacgaa agaggtgat 
      cttggtttt gttccgaaa ccattgaca cgtcgttca 
      actttgtca acacttcga gatattcaa gctcagaca 
      ggtccaagc tttgatgaa tttttggca attggtaaa 
      gaagaggat cttgagcaa caagaaact tctttggtt 
      agttctgaa gaagagtcc aaaggtagt gaaggaact 
      ttggtggag gaggaaagt gatgtgttc tttgacgag 
      gaggaagaa gctgcagac gagtccatg ttggaatat 
      gacgacgtt gaattactt gatgaagaa gatgaggaa 
      attaaagca gctcgtgaa gtagctgca acgaaatcc 
      gccaatgag ttaatagtc gaaagggca ctaagtagt 
      gcagctatt gcgaaaaaa ttgtcggga attggtagt 
      aaagaaaaa tcaagaagc cgaatttct ttcaaagca 
      agtaaagag aaaggggtt ggacaaagt agtaaagaa 
      gaagtggcg gaaaagaaa agtcaagaa aaagaattg 
      ggtaaagca agtcgtgag ggtatgtcc actgataaa 
      ttaaaaagg gtgtctatt ggagagact actgaaatg 
      gttattcct ggagaaaca gcagcgcct ttgccgaaa 
      aatggtgac aaatctgct gagacaagt acttctgaa 
      ccatctaca taa
    • >TC032006 RB
      atgaagaaa attgcactt tgtcgtgct ttgatgaat 
      tttccgttg ttcctgttg gttttttgt ctagcaaag 
      gacggagat gcaggagga agcatccca ggaatagaa 
      ttatcgatg ccgccttcc cacatcgcc ttgagcggc 
      gacctccac atccaaatt acttcatcg tcttttcca 
      ccactaatg ctgcaatta tctcgactc gaaggcaac 
      atcgcccag cccctaacc acttttccc gttctaccc 
      gaagcgcgc gctctttcc ctcaatacg acaattgtg 
      aaaatacct tgcggatac ttttccaaa ggcggccag 
      tattacata cttattaag aagcaacca attggtggc 
      agcaattca actcttgtt ggagacgac gattcggtt 
      atcacacga agtttggac gtacggtgg ccaatgcct 
      caactatcg gtgacaccg gaacatata caaacttat 
      ccggagact ccggtgatg gcgattctc gagtttccg 
      gaagtggtg tgtcctccg gtgacggac agtccgatt 
      agtgcgata ccggagttt tggttagag ttacattat 
      tgcggccat tcgttgctt acctgtgat aatgacgat 
      aacagacag aataattca aatgttcag gtattggat 
      caagtgcgg ggatttgga ggaaggcgg gtgtttaca 
      cttaggtgt gaactcttt ggtttagct ggattttat 
      gcacttttt ctacgaccg actgctact acacaatta 
      ctgcctcat actgctgcc tacgtcaag gccgattgg 
      agcgagcag ttcgtgttt aatgtccat gcccgaagc 
      attctgcct tgtgacggc cacagcggc atcaaagtc 
      ctgttccaa tacccgtcg tgtattctg gcatcaggg 
      gaccgagtc cgagttttc gctcggttg cgagccaat 
      gttgcatca ctggcaccc ccaacatcc ctcgaatat 
      gtggcagaa aagcgagtt atcagagga cagcacagt 
      ttgcacttt gattgtgat ttattcacc gaacgctat 
      gtcgaatat tgcttcgtg tatgtgagc caagcaatc 
      aacgaagct gtcgctgac gtgaggatg gattgcgtg 
      cccaccctt cctgtcaca gagaatgaa agtggtggt 
      tggggtcct tggagccct tggacgcct tgttcaagc 
      acatgcatt gggggtaca agaagtcgt tatcgtttt 
      tgtgactcc ccacctcct cgttacggc gccaagttt 
      tgcgagttg tgctacatc aactacaat atggcgaaa 
      ggtaaaaag aaaggcaag aagaagaag aaagcgcct 
      aaaatcgac ccaaacgcc ttaaccgaa gtcgataaa 
      acattctat gagttaaca ataaccgat ttaaaccga 
      aaattggcc cgcttacgc agtttaacc actgaactc 
      gaggagaaa aacgaacaa ttacaaacc gatttgtcc 
      aaactcgat gaagatcgc aacgacatt atcatctat 
      cttaagcgg atgcttcaa gaacgttct gatgaaata 
      gccgaactt caggaacgt ttgcgtgct ttggaggaa 
      acacgtcaa gatgaaacg gaacaatat gaagcgaaa 
      attaccgat ttggaaaac gaatacaag caaatgcac 
      gaacagtta accagcgag attaaatta cttgaaggt 
      aaattaaat tcgttggaa gaatttcgc attcaaagg 
      gatgagttg atgaaaaaa ttcgaagac caggaaatc 
      gcaatggaa gagcaggag aaacggcac aagaacgaa 
      ttgtatgac attgaacga aaatttatt attgggaaa 
      gataaactg aagaaagat atggaagca aggttactc 
      caactatcg tccgaattt caggaagca actgaacta 
      cgaattgct gccaccact cacagagtg attcgagaa 
      aacattgca attaataac gaactagat gcaatgttg 
      atcactcac caaaaacta cactccgat aatgcgaaa 
      atgaaagac agggatcga actttgcgc caagaaact 
      gaacttcat gaagctgaa aagaagaaa gctttaaat 
      aaggtcagg gttcagttg aaactaatt gatcgatta 
      accactgaa cacgaagat atgaaacaa aaattaacc 
      aaatatgaa acaacagaa caagagatg catcaaact 
      tctgaagaa ctccagaaa acgaaacag gacgtcctt 
      tgcctaaac cacaaaatt aaactcttg gaacaaaac 
      attcacgca ttgaaatgt cgagaaagc tcgctcaag 
      acggagtta agtaacctt gattttgat aacgatcgt 
      ctgacaaca gttcttgcc caagctgtt ttatcaatt 
      aaggaagtt ttggaatta cagtcacga atgtcagat 
      gaagcactg aaagcatca aaacgtgaa aatctccta 
      acccaactt ttcgtttta ttaagtgaa gctgagacg 
      aagaaaggt cgtaaagct tctttggac actattggt 
      tccttgtct gcaacttac gaaagaggt gatcttggt 
      tttgttccg aaaccattg acacgtcgt tcaactttg 
      tcaacactt cgagatatt caagctcag acaggtcca 
      agctttgat gaatttttg gcaattggt aaagaagag 
      gatcttgag caacaagaa acttctttg gttagttct 
      gaagaagag tccaaaggt agtgaagga actttggtg 
      gaggaggaa agtgatgtg ttctttgac gaggaggaa 
      gaagctgca gacgagtcc atgttggaa tatgacgac 
      gttgaatta cttgatgaa gaagatgag gaaattaaa 
      gcagctcgt gaagtagct gcaacgaaa tccgccaat 
      gagttaata gtcgaaagg gcactaagt agtgcagct 
      attgcgaaa aaattgtcg ggaattggt agtaaagaa 
      aaatcaaga agccgaatt tctttcaaa gcaagtaaa 
      gagaaaggg gttggacaa agtagtaaa gaagaagtg 
      gcggaaaag aaaagtcaa gaaaaagaa ttgggtaaa 
      gcaagtcgt gagggtatg tccactgat aaattaaaa 
      agggtgtct attggagag actactgaa atggttatt 
      cctggagaa acagcagcg cctttgccg aaaaatggt 
      gacaaatct gctgagaca agtacttct gaaccatct 
      acataa
    mRNA sequence
    • >TC032006 RA
      cgtccggaa ttaatcgca aagttatgg caagaggaa 
      gttgttgat gaattcgta gtggatgag tgcacgtgc 
      caaaaaact gttcaaaat gttgaatga gttagaaga 
      cggatatga agaaaattg cactttgtc gtgctttga 
      tgaattttc cgttgttcc tgttggttt tttgtctag 
      caaaggacg gagatgcag gaggaagca tcccaggaa 
      tagaattat cgatgccgc cttcccaca tcgccttga 
      gcggcgacc tccacatcc aaattactt catcgtctt 
      ttccaccac taatgctgc aattatctc gactcgaag 
      gcaacatcg cccagcccc taaccactt ttcccgttc 
      tacccgaag cgcgcgctc tttccctca atacgacaa 
      ttgtgaaaa taccttgcg gatactttt ccaaaggcg 
      gccagtatt acatactta ttaagaagc aaccaattg 
      gtggcagca attcaactc ttgttggag acgacgatt 
      cggttatca cacgaagtt tggacgtac ggtggccaa 
      tgcctcaac tatcggtga caccggaac atatacaaa 
      cttatccgg agactccgg tgatggcga ttctcgagt 
      ttccggaag tggtgtgtc ctccggtga cggacagtc 
      cgattagtg cgataccgg agttttggt tagagttac 
      attattgcg gccattcgt tgcttacct gtgataatg 
      acgataaca gacagaata attcaaatg ttcaggtat 
      tggatcaag tgcggggat ttggaggaa ggcgggtgt 
      ttacactta ggtgtgaac tctttggtt tagctggat 
      tttatgcac tttttctac gaccgactg ctactacac 
      aattactgc ctcatactg ctgcctacg tcaaggccg 
      attggagcg agcagttcg tgtttaatg tccatgccc 
      gaagcattc tgccttgtg acggccaca gcggcatca 
      aagtcctgt tccaatacc cgtcgtgta ttctggcat 
      caggggacc gagtccgag ttttcgctc ggttgcgag 
      ccaatgttg catcactgg cacccccaa catccctcg 
      aatatgtgg cagaaaagc gagttatca gaggacagc 
      acagtttgc actttgatt gtgatttat tcaccgaac 
      gctatgtcg aatattgct tcgtgtatg tgagccaag 
      caatcaacg aagctgtcg ctgacgtga ggatggatt 
      gcgtgccca cccttcctg tcacagaga atgaaagtg 
      gtggttggg gtccttgga gcccttgga cgccttgtt 
      caagcacat gcattgggg gtacaagaa gtcgttatc 
      gtttttgtg actccccac ctcctcgtt acggcgcca 
      agttttgcg agttgtgct acatcaact acaatatgg 
      cgaaaggta aaaagaaag gcaagaaga agaagaaag 
      cgcctaaaa tcgacccaa acgccttaa ccgaagtcg 
      ataaaacat tctatgagt taacaataa ccgatttaa 
      accgaaaat tggcccgct tacgcagtt taaccactg 
      aactcgagg agaaaaacg aacaattac aaaccgatt 
      tgtccaaac tcgatgaag atcgcaacg acattatca 
      tctatctta agcggatgc ttcaagaac gttctgatg 
      aaatagccg aacttcagg aacgtttgc gtgctttgg 
      aggaaacac gtcaagatg aaacggaac aatatgaag 
      cgaaaatta ccgatttgg aaaacgaat acaagcaaa 
      tgcacgaac agttaacca gcgagatta aattacttg 
      aaggtaaat taaattcgt tggaagaat ttcgcattc 
      aaagggatg agttgatga aaaaattcg aagaccagg 
      aaatcgcaa tggaagagc aggagaaac ggcacaaga 
      acgaattgt atgacattg aacgaaaat ttattattg 
      ggaaagata aactgaaga aagatatgg aagcaaggt 
      tactccaac tatcgtccg aatttcagg aagcaactg 
      aactacgaa ttgctgcca ccactcaca gagtgattc 
      gagaaaaca ttgcaatta ataacgaac tagatgcaa 
      tgttgatca ctcaccaaa aactacact ccgataatg 
      cgaaaatga aagacaggg atcgaactt tgcgccaag 
      aaactgaac ttcatgaag ctgaaaaga agaaagctt 
      taaataagg tcagggttc agttgaaac taattgatc 
      gattaacca ctgaacacg aagatatga aacaaaaat 
      taaccaaat atgaaacaa cagaacaag agatgcatc 
      aaacttctg aagaactcc agaaaacga aacaggacg 
      tcctttgcc taaaccaca aaattaaac tcttggaac 
      aaaacattc acgcattga aatgtcgag aaagctcgc 
      tcaagacgg agttaagta accttgatt ttgataacg 
      atcgtctga caacagttc ttgcccaag ctgttttat 
      caattaagg aagttttgg aattacagt cacgaatgt 
      cagatgaag cactgaaag catcaaaac gtgaaaatc 
      tcctaaccc aacttttcg ttttattaa gtgaagctg 
      agacgaaga aaggtcgta aagcttctt tggacacta 
      ttggttcct tgtctgcaa cttacgaaa gaggtgatc 
      ttggttttg ttccgaaac cattgacac gtcgttcaa 
      ctttgtcaa cacttcgag atattcaag ctcagacag 
      gtccaagct ttgatgaat ttttggcaa ttggtaaag 
      aagaggatc ttgagcaac aagaaactt ctttggtta 
      gttctgaag aagagtcca aaggtagtg aaggaactt 
      tggtggagg aggaaagtg atgtgttct ttgacgagg 
      aggaagaag ctgcagacg agtccatgt tggaatatg 
      acgacgttg aattacttg atgaagaag atgaggaaa 
      ttaaagcag ctcgtgaag tagctgcaa cgaaatccg 
      ccaatgagt taatagtcg aaagggcac taagtagtg 
      cagctattg cgaaaaaat tgtcgggaa ttggtagta 
      aagaaaaat caagaagcc gaatttctt tcaaagcaa 
      gtaaagaga aaggggttg gacaaagta gtaaagaag 
      aagtggcgg aaaagaaaa gtcaagaaa aagaattgg 
      gtaaagcaa gtcgtgagg gtatgtcca ctgataaat 
      taaaaaggg tgtctattg gagagacta ctgaaatgg 
      ttattcctg gagaaacag cagcgcctt tgccgaaaa 
      atggtgaca aatctgctg agacaagta cttctgaac 
      catctacat aaatatgtt ttgattttt tttaataaa 
      gactgcttt accat
    • >TC032006 RB
      cgtccggaa ttaatcgca aagttatgg caagaggaa 
      gttgttgat gaattcgta gtggatgag tgcacgtgc 
      caaaaaact gttcaaaat gttgaatga gttagaaga 
      cggatatga agaaaattg cactttgtc gtgctttga 
      tgaattttc cgttgttcc tgttggttt tttgtctag 
      caaaggacg gagatgcag gaggaagca tcccaggaa 
      tagaattat cgatgccgc cttcccaca tcgccttga 
      gcggcgacc tccacatcc aaattactt catcgtctt 
      ttccaccac taatgctgc aattatctc gactcgaag 
      gcaacatcg cccagcccc taaccactt ttcccgttc 
      tacccgaag cgcgcgctc tttccctca atacgacaa 
      ttgtgaaaa taccttgcg gatactttt ccaaaggcg 
      gccagtatt acatactta ttaagaagc aaccaattg 
      gtggcagca attcaactc ttgttggag acgacgatt 
      cggttatca cacgaagtt tggacgtac ggtggccaa 
      tgcctcaac tatcggtga caccggaac atatacaaa 
      cttatccgg agactccgg tgatggcga ttctcgagt 
      ttccggaag tggtgtgtc ctccggtga cggacagtc 
      cgattagtg cgataccgg agttttggt tagagttac 
      attattgcg gccattcgt tgcttacct gtgataatg 
      acgataaca gacagaata attcaaatg ttcaggtat 
      tggatcaag tgcggggat ttggaggaa ggcgggtgt 
      ttacactta ggtgtgaac tctttggtt tagctggat 
      tttatgcac tttttctac gaccgactg ctactacac 
      aattactgc ctcatactg ctgcctacg tcaaggccg 
      attggagcg agcagttcg tgtttaatg tccatgccc 
      gaagcattc tgccttgtg acggccaca gcggcatca 
      aagtcctgt tccaatacc cgtcgtgta ttctggcat 
      caggggacc gagtccgag ttttcgctc ggttgcgag 
      ccaatgttg catcactgg cacccccaa catccctcg 
      aatatgtgg cagaaaagc gagttatca gaggacagc 
      acagtttgc actttgatt gtgatttat tcaccgaac 
      gctatgtcg aatattgct tcgtgtatg tgagccaag 
      caatcaacg aagctgtcg ctgacgtga ggatggatt 
      gcgtgccca cccttcctg tcacagaga atgaaagtg 
      gtggttggg gtccttgga gcccttgga cgccttgtt 
      caagcacat gcattgggg gtacaagaa gtcgttatc 
      gtttttgtg actccccac ctcctcgtt acggcgcca 
      agttttgcg agttgtgct acatcaact acaatatgg 
      cgaaaggta aaaagaaag gcaagaaga agaagaaag 
      cgcctaaaa tcgacccaa acgccttaa ccgaagtcg 
      ataaaacat tctatgagt taacaataa ccgatttaa 
      accgaaaat tggcccgct tacgcagtt taaccactg 
      aactcgagg agaaaaacg aacaattac aaaccgatt 
      tgtccaaac tcgatgaag atcgcaacg acattatca 
      tctatctta agcggatgc ttcaagaac gttctgatg 
      aaatagccg aacttcagg aacgtttgc gtgctttgg 
      aggaaacac gtcaagatg aaacggaac aatatgaag 
      cgaaaatta ccgatttgg aaaacgaat acaagcaaa 
      tgcacgaac agttaacca gcgagatta aattacttg 
      aaggtaaat taaattcgt tggaagaat ttcgcattc 
      aaagggatg agttgatga aaaaattcg aagaccagg 
      aaatcgcaa tggaagagc aggagaaac ggcacaaga 
      acgaattgt atgacattg aacgaaaat ttattattg 
      ggaaagata aactgaaga aagatatgg aagcaaggt 
      tactccaac tatcgtccg aatttcagg aagcaactg 
      aactacgaa ttgctgcca ccactcaca gagtgattc 
      gagaaaaca ttgcaatta ataacgaac tagatgcaa 
      tgttgatca ctcaccaaa aactacact ccgataatg 
      cgaaaatga aagacaggg atcgaactt tgcgccaag 
      aaactgaac ttcatgaag ctgaaaaga agaaagctt 
      taaataagg tcagggttc agttgaaac taattgatc 
      gattaacca ctgaacacg aagatatga aacaaaaat 
      taaccaaat atgaaacaa cagaacaag agatgcatc 
      aaacttctg aagaactcc agaaaacga aacaggacg 
      tcctttgcc taaaccaca aaattaaac tcttggaac 
      aaaacattc acgcattga aatgtcgag aaagctcgc 
      tcaagacgg agttaagta accttgatt ttgataacg 
      atcgtctga caacagttc ttgcccaag ctgttttat 
      caattaagg aagttttgg aattacagt cacgaatgt 
      cagatgaag cactgaaag catcaaaac gtgaaaatc 
      tcctaaccc aacttttcg ttttattaa gtgaagctg 
      agacgaaga aaggtcgta aagcttctt tggacacta 
      ttggttcct tgtctgcaa cttacgaaa gaggtgatc 
      ttggttttg ttccgaaac cattgacac gtcgttcaa 
      ctttgtcaa cacttcgag atattcaag ctcagacag 
      gtccaagct ttgatgaat ttttggcaa ttggtaaag 
      aagaggatc ttgagcaac aagaaactt ctttggtta 
      gttctgaag aagagtcca aaggtagtg aaggaactt 
      tggtggagg aggaaagtg atgtgttct ttgacgagg 
      aggaagaag ctgcagacg agtccatgt tggaatatg 
      acgacgttg aattacttg atgaagaag atgaggaaa 
      ttaaagcag ctcgtgaag tagctgcaa cgaaatccg 
      ccaatgagt taatagtcg aaagggcac taagtagtg 
      cagctattg cgaaaaaat tgtcgggaa ttggtagta 
      aagaaaaat caagaagcc gaatttctt tcaaagcaa 
      gtaaagaga aaggggttg gacaaagta gtaaagaag 
      aagtggcgg aaaagaaaa gtcaagaaa aagaattgg 
      gtaaagcaa gtcgtgagg gtatgtcca ctgataaat 
      taaaaaggg tgtctattg gagagacta ctgaaatgg 
      ttattcctg gagaaacag cagcgcctt tgccgaaaa 
      atggtgaca aatctgctg agacaagta cttctgaac 
      catctacat aaatatgtt ttgattttt tttaataaa 
      gactgcttt accat