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Gene information
General information
TC034282
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005219
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1799168..1802513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034282
    iBeetle screen iB_03923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034282 RA
      MYIRGKNHI PTVAEEQQL FAAIRENDL NKVRDIIAT 
      GYNLNCQHF SSTPLIVAI KHKNLDMIK LLLDSGANV 
      NNGTRDGFL PLSAAISSN CPIEIIKVL ISYGANVNH 
      IGVDFFNYE NLLPLVIKL TPLSWAIVK NNKQVAQLL 
      LENGADING RNDDGTSAL RLSQIFNRL EFLQKNPHL 
      AQPHPVVEQ TYISKNLEG CTPLTAAIV SGKLDDAKA 
      LLEEVDSCK ADSNKQTPL LAAVRCENL EAVKLLLNY 
      KANLTIPTA FLEAINLKC LEIVKYLLE KGANVNKKY 
      RDGCTPLIK AIENESLKI VKELLNSGA EVNLANSEG 
      MTPLALAAN KRSILIVDL LVETGADIN LARQELEEF 
      AKDDEDFDT YYENLKEKR EKRDFRYGA HEAAD
    CDS sequence
    • >TC034282 RA
      atgtatatt agggggaaa aatcatata ccgaccgtc 
      gcagaagaa cagcaatta tttgcggca atacgtgaa 
      aatgatttg aacaaggtc agagacatt atcgccaca 
      ggatataat ctaaattgt caacacttc tcttccacg 
      ccactaatt gttgccatt aagcataaa aatttggat 
      atgattaaa ctattattg gatagtggc gccaatgtt 
      aataatgga accagagat ggtttttta ccattaagt 
      gcagccatt tccagtaac tgtccaata gaaattatc 
      aaagtattg ataagttat ggagcaaat gtaaaccac 
      attggagta gactttttc aactatgaa aatctattg 
      ccattagta ataaaacta actccactt tcatgggca 
      atagtgaaa aataataag caagtggct cagttattg 
      ttagaaaat ggcgcagac atcaatgga agaaatgac 
      gatgggaca tccgctctc agactttca caaattttc 
      aatcgttta gaattctta caaaaaaat ccacatctt 
      gctcaaccg catccagtt gttgaacaa acatacatc 
      tcaaaaaat ctagagggt tgtacacct ttgacagca 
      gccattgtg tccggaaaa ctagacgac gccaaagcg 
      cttttagaa gaagtcgat tcttgcaag gcagattcc 
      aacaaacaa actcctctt cttgcagca gtgcgttgt 
      gaaaatctc gaagctgtg aaacttttg ctaaattat 
      aaagcaaat ttaacgatt ccgactgct tttctcgaa 
      gctattaac ttgaagtgt ttggagatt gttaaatat 
      ttgttagaa aaaggagcc aatgtcaat aaaaagtac 
      cgcgacggt tgcacacct ctgatcaaa gctatagaa 
      aatgaaagt ttaaaaatt gtgaaagag cttttaaac 
      agtggtgct gaggtgaac ttggccaac agcgaagga 
      atgactcct ttggcacta gcagcaaat aaaagaagt 
      attcttatt gtggatctt ttagtcgag acaggagct 
      gatatcaat ttggcacga caagaactt gaagaattt 
      gcaaaagat gatgaagat tttgacacc tattacgaa 
      aacttgaaa gagaaaaga gagaaaaga gattttcgt 
      tacggagct cacgaagct gcggactag
    mRNA sequence
    • >TC034282 RA
      tggttgatt tgtggcatt ttacaattt ttgtaacaa 
      aatgtatat tagggggaa aaatcatat accgaccgt 
      cgcagaaga acagcaatt atttgcggc aatacgtga 
      aaatgattt gaacaaggt cagagacat tatcgccac 
      aggatataa tctaaattg tcaacactt ctcttccac 
      gccactaat tgttgccat taagcataa aaatttgga 
      tatgattaa actattatt ggatagtgg cgccaatgt 
      taataatgg aaccagaga tggtttttt accattaag 
      tgcagccat ttccagtaa ctgtccaat agaaattat 
      caaagtatt gataagtta tggagcaaa tgtaaacca 
      cattggagt agacttttt caactatga aaatctatt 
      gccattagt aataaaact aactccact ttcatgggc 
      aatagtgaa aaataataa gcaagtggc tcagttatt 
      gttagaaaa tggcgcaga catcaatgg aagaaatga 
      cgatgggac atccgctct cagactttc acaaatttt 
      caatcgttt agaattctt acaaaaaaa tccacatct 
      tgctcaacc gcatccagt tgttgaaca aacatacat 
      ctcaaaaaa tctagaggg ttgtacacc tttgacagc 
      agccattgt gtccggaaa actagacga cgccaaagc 
      gcttttaga agaagtcga ttcttgcaa ggcagattc 
      caacaaaca aactcctct tcttgcagc agtgcgttg 
      tgaaaatct cgaagctgt gaaactttt gctaaatta 
      taaagcaaa tttaacgat tccgactgc ttttctcga 
      agctattaa cttgaagtg tttggagat tgttaaata 
      tttgttaga aaaaggagc caatgtcaa taaaaagta 
      ccgcgacgg ttgcacacc tctgatcaa agctataga 
      aaatgaaag tttaaaaat tgtgaaaga gcttttaaa 
      cagtggtgc tgaggtgaa cttggccaa cagcgaagg 
      aatgactcc tttggcact agcagcaaa taaaagaag 
      tattcttat tgtggatct tttagtcga gacaggagc 
      tgatatcaa tttggcacg acaagaact tgaagaatt 
      tgcaaaaga tgatgaaga ttttgacac ctattacga 
      aaacttgaa agagaaaag agagaaaag agattttcg 
      ttacggagc tcacgaagc tgcggacta gaccctgga 
      gttctatat ctttgtagt taattgatt attttatct 
      tctaacaaa taaatattt tggcccttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY