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Gene information
General information
TC005211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2262992..2270327
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC005211
      iBeetle screen iB_03922

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005211 RA
        MQTCSSNKE ETCSEISNI AADAKDSLL PSKSKHLYE 
ETYNAYRKW RSNKKIDTT CEDTILAYF SSELSRYKS 
SSLWSKYSM LRSTINLRE GIDISKFPS VIPYLKRKG 
EGHKPKKSL ILSKDHIDE FLRKADTKE HLFNKVVLI 
FGVAGACRR QELVTLTTT CVQDCKTHF LIKLEDTKT 
KVDRCFIIT AGKLENVNL LELVRRYME IRPTKTPHN 
RFFINYTKE KCTIQPVGI HKIGGVPAI VAKYLGLEN 
ASSYTGHCF RRSSASLLA NAGATMERI KRHGGWRST 
TVAEGYIEE CENTKIKVA NLILGEEPL ACNSISSEN 
HEKFTSLRG TESEAKNNE VVGMNICGN NNSVINVNH 
YYNKQ
      CDS sequence
      • >TC005211 RA
        atgcaaacg tgcagttca aataaagaa gaaacttgc 
agcgaaata tcaaatatt gctgctgat gcgaaagat 
tctctgtta ccatcaaaa tccaagcat ttgtacgaa 
gaaacatat aatgcgtat cggaaatgg cgttctaat 
aagaaaatt gatacaact tgcgaagat accattcta 
gcgtacttc agcagcgag ctgtcccgg tataaaagt 
tcatcatta tggagcaaa tattctatg ctaagatca 
accatcaat ctacgagag ggaattgac attagtaaa 
tttccaagc gtcatacct tatttgaaa cgaaaaggt 
gaaggacac aaaccaaaa aaatctctt atattgagt 
aaagaccat atagacgaa tttttgaga aaagctgac 
accaaggaa catttattt aataaggtt gttctcata 
tttggagta gccggtgca tgtagacgt caagagcta 
gtgacatta acgacaaca tgtgttcaa gactgcaaa 
acccatttt ttaattaag ctggaagac accaaaacc 
aaagtggat cgatgtttt ataataaca gcaggtaaa 
ctcgaaaat gttaatcta cttgaactt gtcagaaga 
tatatggaa attcgaccc accaagaca ccgcacaac 
agatttttt atcaactac accaaggaa aaatgtacc 
attcaacca gttggaatc cacaaaatt gggggtgtg 
cctgctata gtggccaag tatttgggt cttgaaaat 
gcttcttca tatacaggt cactgcttc agacgtagc 
tcagcttct cttctggca aatgcaggg gccactatg 
gaaaggata aaaagacac ggaggttgg cgttcaact 
acagtggca gaaggttat atcgaagaa tgtgaaaat 
actaaaatt aaggttgca aacttgatc ttgggtgaa 
gaaccattg gcctgcaat agcatctct tcggaaaat 
cacgaaaaa tttacaagt ttgcgtgga accgagagt 
gaagcaaaa aacaacgaa gttgtagga atgaatatt 
tgtggcaac aataatagt gttataaat gtaaatcat 
tattacaac aagcaatag
      mRNA sequence
      • >TC005211 RA
        tacgaagat tgctacata aagccttca ggtcaagac 
attttaaag ttaaacaaa atgcaaacg tgcagttca 
aataaagaa gaaacttgc agcgaaata tcaaatatt 
gctgctgat gcgaaagat tctctgtta ccatcaaaa 
tccaagcat ttgtacgaa gaaacatat aatgcgtat 
cggaaatgg cgttctaat aagaaaatt gatacaact 
tgcgaagat accattcta gcgtacttc agcagcgag 
ctgtcccgg tataaaagt tcatcatta tggagcaaa 
tattctatg ctaagatca accatcaat ctacgagag 
ggaattgac attagtaaa tttccaagc gtcatacct 
tatttgaaa cgaaaaggt gaaggacac aaaccaaaa 
aaatctctt atattgagt aaagaccat atagacgaa 
tttttgaga aaagctgac accaaggaa catttattt 
aataaggtt gttctcata tttggagta gccggtgca 
tgtagacgt caagagcta gtgacatta acgacaaca 
tgtgttcaa gactgcaaa acccatttt ttaattaag 
ctggaagac accaaaacc aaagtggat cgatgtttt 
ataataaca gcaggtaaa ctcgaaaat gttaatcta 
cttgaactt gtcagaaga tatatggaa attcgaccc 
accaagaca ccgcacaac agatttttt atcaactac 
accaaggaa aaatgtacc attcaacca gttggaatc 
cacaaaatt gggggtgtg cctgctata gtggccaag 
tatttgggt cttgaaaat gcttcttca tatacaggt 
cactgcttc agacgtagc tcagcttct cttctggca 
aatgcaggg gccactatg gaaaggata aaaagacac 
ggaggttgg cgttcaact acagtggca gaaggttat 
atcgaagaa tgtgaaaat actaaaatt aaggttgca 
aacttgatc ttgggtgaa gaaccattg gcctgcaat 
agcatctct tcggaaaat cacgaaaaa tttacaagt 
ttgcgtgga accgagagt gaagcaaaa aacaacgaa 
gttgtagga atgaatatt tgtggcaac aataatagt 
gttataaat gtaaatcat tattacaac aagcaatag 
tatgtttat atggttaca atgttacaa agttgatat 
gtatgtatt attataact tcaaataaa ttttgacat 
ttctg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY