Status
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Gene information
General information
TC031886
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004098,TC004099
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452165.1: 2792..6884
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031886
      iBeetle screen iB_03921

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031886 RA
        PPLTLEAIM GAMKTLLAP LSERLQRLE EAVFSDKAS 
        EAGSTSTFT GFSAHSLKS GESQDFMET EGFSPVKTG 
        KKRGAPSPS PVDNEIDTG NRFSSLSNE DDFEPTPSD 
        VENGEESEM EERRKKKKP SSPKPSPRI ATWASVAAA 
        PKCSTSAAP RISIPAAPR VSPPTAPQG STPTAPISK 
        APRIPPIFL RDAGKWQRV SSVANERKY GFTKARSVS 
        DQIRIQPTT VADFRSFTK FMDEEKIPY HTFTLPEEK 
        NTRVVLRGI PVQVSMEAV LQDLKLQGF NPVCVHRMH 
        AGKRQLPLV LVEAPLSQT KDVWQIKTV CSLMVKVEK 
        PRKSGKAAQ CHRCQRFFH AQRNCTAEH RCVKCGEAH 
        DTKVCTKES KEPPKCANF PQAAENRHP QDNRPRQDK 
        AAPAKAAPA KAVTPKAAA PKKAAAPKP TAAPKVVAP 
        KANPTATKG KGGAKKTAQ QRQTVTFGL QRNQIATAV 
        AMAKSPEEL AEKLSAFVA FWNANGLRA ARDELEEFV 
        DRLQLDIVL VCETKLQPQ TTDPKIRGF TLHRADRGI 
        GPGGGTAIF VSNRIKHSV LATPDLRNM EAVGINVAT 
        ANGPLRLFA CYNRPQIPI LEEDLQTLF DGNTPTIAA 
        GDFNAKHIN WGSRRSNRN GNFLNGFTD QNLDISVMA 
        PVEPTFYRN SDGAADILD VAIVKNVVH QVRLTAIND 
        LSSDHNPVL MQIGNEAND PIVCRYTSV DWRKFTKHL 
        VNNFGTIPP IRSKEEIDE AVQTFETKI RDAIASATR 
        ERRTPAPRL EISWEIRDL IRAKRRARR IAQRTGFPV 
        DRAEANRLR WEVRKALSD FRNERWEAK LQSLTTEDN 
        SVWRMSRVL RSDRKPLPP IHSENGIVF TDEEKAEAF 
        ALSMSRQCS LNLTNADLD HVEEIEDHV ESIATEDPD 
        EPLTPTTPE EVSGVIRKL KKRKASGPD EISNRALKN 
        LPLKVIVEL TGILNAMFS FRYFPQRWK MATVIFIPK 
        PGKDPKFPQ NHRPISLLS AVGKVAERL IRTRLLQLT 
        QERHIVPDE QFGFRSNHS TTDQLLRVV EHASISIER 
        KQVTGAVFL DVAKAFDAV WHDGLIHKL HQTGIPLAM 
        VQMIRSFLV GRRFQVRIN NSVSDPQDL EAGVPQGSV 
        LSPLLYSIF THDIPKTDR TTLAIYADD TAILTRSKQ 
        PYMATRYLQ ESVECIENW CRRWLINVN PDKSRALLL 
        ARRRVSPDG FVRMFNADI PWSDQVKYL GVILDKKLS 
        FGPHLDYAL AKGKMATGM LRSLVCRRS ALSIDNKLL 
        LYKSVIRPT MTYASVAWA FAPCKTRMH KLQTFQNKF 
        LRQAFNAPW FVRNNQLHR EVKMPTMEE FFRETAS
      CDS sequence
      • >TC031886 RA
        cccccattg acactcgaa gccatcatg ggtgccatg 
        aagactctt ctggcaccc ctcagcgag agactccag 
        cgtctcgag gaggcagtc ttttcagac aaagcctcc 
        gaagccggt tccacctcg actttcacc gggttttca 
        gcacattcg ctgaaatcc ggggaatcc caggatttc 
        atggaaacc gagggcttc agcccagtg aaaactggg 
        aagaagcga ggagctcca tccccatcc cccgtcgac 
        aatgagatc gacacaggg aacaggttc tcgagcctg 
        tcgaacgag gatgatttc gagccgacc ccatcggat 
        gtcgaaaat ggggaagaa agtgagatg gaagaaagg 
        aggaagaag aagaagcct tcttcgccc aagccctct 
        cccaggatc gccacttgg gcttccgtc gcggcggcc 
        ccaaagtgc tccacatcg gcagccccc cggatctcc 
        atcccggca gccccccgg gtctctccc ccgacagcc 
        ccccagggc tccaccccg acagccccc atttcaaag 
        gccccacga attccgccg attttcttg agagacgca 
        ggaaaatgg caacgcgtt tcgtccgtg gccaatgaa 
        cggaagtac ggtttcaca aaggcgagg agcgtgagt 
        gatcagatc cgaattcag ccaactacc gtagctgat 
        ttcagatca ttcacgaaa ttcatggac gaggagaaa 
        atcccgtac catactttc accctccct gaggagaag 
        aacaccagg gtcgtcctg cgcgggatt ccagtccag 
        gtatccatg gaggcagtg ctccaggac ttgaagctc 
        cagggcttc aatcctgtc tgcgtccac cggatgcac 
        gccggaaag aggcaactc ccgttggtg ctcgtcgag 
        gcaccactc agccagaca aaggatgtc tggcaaatc 
        aagaccgtc tgcagcctg atggtcaag gttgaaaaa 
        ccgaggaaa tcaggcaaa gcagcacag tgccatcgg 
        tgccagcga tttttccac gcacagagg aactgcacg 
        gcagaacac cgatgtgtc aagtgcggg gaagcacat 
        gacacaaaa gtgtgcacg aaggagagc aaggagcct 
        cccaagtgt gccaatttt ccccaagct gcagaaaac 
        cgccacccc caggacaac cgcccccgc caggacaag 
        gccgccccc gccaaggcc gcccccgcc aaggccgtc 
        acccccaag gcagcagcc cccaaaaag gccgccgct 
        cccaagcca acggccgcc cccaaggtc gtcgctccc 
        aaggcaaac cccaccgcc accaaaggc aaaggaggc 
        gccaaaaag accgctcaa cagcgtcaa acggtgacc 
        tttggtttg cagaggaac cagattgcc acggccgtg 
        gcaatggca aagagcccg gaagagttg gctgaaaaa 
        ctctcagcc ttcgtggcg ttctggaac gccaatggt 
        cttcgagcg gcccgggac gaactcgaa gaattcgtc 
        gacaggctg caacttgac atcgtactt gtgtgcgaa 
        acaaagttg caacctcaa acgacggac ccaaaaatt 
        cggggtttc actctccac agggcggac agagggatc 
        ggcccagga ggaggaacg gcaattttt gtaagtaac 
        agaataaaa cattctgtt ctagcaaca cccgatctt 
        cgcaacatg gaagccgtg ggcatcaac gtagccact 
        gcaaatggg cccctgcgc ctgtttgca tgctacaac 
        cgaccacaa attccaatt ctcgaagag gacctgcag 
        acacttttc gacggcaac acaccaacg atcgctgct 
        ggtgatttt aacgccaag cacatcaac tggggaagc 
        cgccgttcg aacagaaac ggaaacttc ctcaacggc 
        ttcacggat caaaacctg gacatctcc gtcatggct 
        ccggtagaa cccacgttc taccgaaac tcggacggt 
        gctgcagac atccttgac gtagcaatc gtcaaaaac 
        gtcgtgcac caggttcgg ctgacggca ataaacgac 
        ctctcttcg gatcataac cccgtcctg atgcaaatc 
        gggaacgag gcaaacgat ccgattgta tgtcgttat 
        acctcagtg gattggagg aagttcacc aagcacctg 
        gtgaacaac ttcggaaca atcccaccg atcagatcc 
        aaagaagaa atcgacgag gcggtccaa accttcgag 
        acgaagatc cgggacgcc atcgcctcc gccaccagg 
        gaacggaga actccggcc ccaagactg gagatttca 
        tgggaaatc agggacctc atcagggca aaaaggcga 
        gccagacgg attgcacaa cgtaccggt tttccggtg 
        gaccgtgca gaagccaac cggctgaga tgggaggtg 
        agaaaagca ctttctgat ttcagaaac gagagatgg 
        gaggccaag cttcaatct ctcaccacc gaggacaac 
        tccgtctgg agaatgtcg agggtgctg agatctgac 
        cgaaaaccg cttccacca atccacagt gagaacggg 
        atcgtcttc accgacgag gagaaagca gaagcgttc 
        gcgttgagc atgagcaga caatgctca ctcaacctc 
        acgaatgcg gacctggac cacgtcgaa gagatcgag 
        gatcacgtg gaatccatc gccaccgaa gacccagat 
        gaacccctg accccgaca acccccgaa gaagtttcc 
        ggagtgatt cggaaactg aagaaacgg aaggcatcc 
        ggaccagac gaaatcagc aacagggct ctcaagaac 
        ctcccgctg aaagtcatc gtcgaactg acaggtatt 
        ttaaatgca atgttcagt ttcagatat tttccacag 
        cgttggaaa atggcaacg gtcattttc atcccgaaa 
        ccggggaag gacccaaag tttccccag aaccacagg 
        ccaatcagc ttgctgtcg gccgtggga aaggtcgca 
        gaacgtctc atccgcacc cgcctcctc cagctcacc 
        caggagcgg cacatcgtc cccgatgaa caattcggc 
        tttcggtcg aatcattca acaactgat cagctacta 
        cgagtcgta gagcacgct tccatcagt atcgagcgc 
        aagcaagtt actggtgca gtgtttctc gacgtggct 
        aaagcattc gatgcggtt tggcatgat gggcttatt 
        cataagctc catcagact ggcattccg ctcgcaatg 
        gttcaaatg atcagatcg tttctcgtt ggacgccgt 
        ttccaggtg agaatcaat aactctgtt tcagatccc 
        caggacctg gaagccgga gtccctcag ggctccgtt 
        ctttcacct ctcctttac tccattttc acccacgac 
        atcccaaaa actgaccgt acgacactg gcaatatac 
        gcagatgac acagcgatt ctcaccaga tcgaagcaa 
        ccctacatg gctacccgc tacttgcag gagtctgtg 
        gaatgcatc gaaaactgg tgtcgcagg tggctcata 
        aacgtcaac cccgacaaa agccgagcg cttttgttg 
        gcacgacgt agagtcagc cctgatggg tttgttcga 
        atgttcaac gcggatatt ccctggtct gaccaggtc 
        aaatatctc ggggtcatt ctggacaaa aaactctcc 
        ttcggccca catcttgac tacgcactc gcgaagggc 
        aagatggcc acgggaatg ctgagatct ctcgtatgt 
        cggcggagc gcgttgtca attgacaac aagctcttg 
        ttgtacaaa tcagtgatc cgacctacc atgacttat 
        gcttctgtg gcttgggca ttcgcgccc tgtaagact 
        cggatgcat aagttacaa actttccaa aacaaattt 
        ctccgtcaa gcattcaac gccccgtgg tttgttcgc 
        aacaaccaa ttgcaccga gaggtgaag atgccgacg 
        atggaggaa ttcttccgt gagaccgca agcga
      mRNA sequence
      • >TC031886 RA
        tcccccatt gacactcga agccatcat gggtgccat 
        gaagactct tctggcacc cctcagcga gagactcca 
        gcgtctcga ggaggcagt cttttcaga caaagcctc 
        cgaagccgg ttccacctc gactttcac cgggttttc 
        agcacattc gctgaaatc cggggaatc ccaggattt 
        catggaaac cgagggctt cagcccagt gaaaactgg 
        gaagaagcg aggagctcc atccccatc ccccgtcga 
        caatgagat cgacacagg gaacaggtt ctcgagcct 
        gtcgaacga ggatgattt cgagccgac cccatcgga 
        tgtcgaaaa tggggaaga aagtgagat ggaagaaag 
        gaggaagaa gaagaagcc ttcttcgcc caagccctc 
        tcccaggat cgccacttg ggcttccgt cgcggcggc 
        cccaaagtg ctccacatc ggcagcccc ccggatctc 
        catcccggc agccccccg ggtctctcc cccgacagc 
        cccccaggg ctccacccc gacagcccc catttcaaa 
        ggccccacg aattccgcc gattttctt gagagacgc 
        aggaaaatg gcaacgcgt ttcgtccgt ggccaatga 
        acggaagta cggtttcac aaaggcgag gagcgtgag 
        tgatcagat ccgaattca gccaactac cgtagctga 
        tttcagatc attcacgaa attcatgga cgaggagaa 
        aatcccgta ccatacttt caccctccc tgaggagaa 
        gaacaccag ggtcgtcct gcgcgggat tccagtcca 
        ggtatccat ggaggcagt gctccagga cttgaagct 
        ccagggctt caatcctgt ctgcgtcca ccggatgca 
        cgccggaaa gaggcaact cccgttggt gctcgtcga 
        ggcaccact cagccagac aaaggatgt ctggcaaat 
        caagaccgt ctgcagcct gatggtcaa ggttgaaaa 
        accgaggaa atcaggcaa agcagcaca gtgccatcg 
        gtgccagcg atttttcca cgcacagag gaactgcac 
        ggcagaaca ccgatgtgt caagtgcgg ggaagcaca 
        tgacacaaa agtgtgcac gaaggagag caaggagcc 
        tcccaagtg tgccaattt tccccaagc tgcagaaaa 
        ccgccaccc ccaggacaa ccgcccccg ccaggacaa 
        ggccgcccc cgccaaggc cgcccccgc caaggccgt 
        cacccccaa ggcagcagc ccccaaaaa ggccgccgc 
        tcccaagcc aacggccgc ccccaaggt cgtcgctcc 
        caaggcaaa ccccaccgc caccaaagg caaaggagg 
        cgccaaaaa gaccgctca acagcgtca aacggtgac 
        ctttggttt gcagaggaa ccagattgc cacggccgt 
        ggcaatggc aaagagccc ggaagagtt ggctgaaaa 
        actctcagc cttcgtggc gttctggaa cgccaatgg 
        tcttcgagc ggcccggga cgaactcga agaattcgt 
        cgacaggct gcaacttga catcgtact tgtgtgcga 
        aacaaagtt gcaacctca aacgacgga cccaaaaat 
        tcggggttt cactctcca cagggcgga cagagggat 
        cggcccagg aggaggaac ggcaatttt tgtaagtaa 
        cagaataaa acattctgt tctagcaac acccgatct 
        tcgcaacat ggaagccgt gggcatcaa cgtagccac 
        tgcaaatgg gcccctgcg cctgtttgc atgctacaa 
        ccgaccaca aattccaat tctcgaaga ggacctgca 
        gacactttt cgacggcaa cacaccaac gatcgctgc 
        tggtgattt taacgccaa gcacatcaa ctggggaag 
        ccgccgttc gaacagaaa cggaaactt cctcaacgg 
        cttcacgga tcaaaacct ggacatctc cgtcatggc 
        tccggtaga acccacgtt ctaccgaaa ctcggacgg 
        tgctgcaga catccttga cgtagcaat cgtcaaaaa 
        cgtcgtgca ccaggttcg gctgacggc aataaacga 
        cctctcttc ggatcataa ccccgtcct gatgcaaat 
        cgggaacga ggcaaacga tccgattgt atgtcgtta 
        tacctcagt ggattggag gaagttcac caagcacct 
        ggtgaacaa cttcggaac aatcccacc gatcagatc 
        caaagaaga aatcgacga ggcggtcca aaccttcga 
        gacgaagat ccgggacgc catcgcctc cgccaccag 
        ggaacggag aactccggc cccaagact ggagatttc 
        atgggaaat cagggacct catcagggc aaaaaggcg 
        agccagacg gattgcaca acgtaccgg ttttccggt 
        ggaccgtgc agaagccaa ccggctgag atgggaggt 
        gagaaaagc actttctga tttcagaaa cgagagatg 
        ggaggccaa gcttcaatc tctcaccac cgaggacaa 
        ctccgtctg gagaatgtc gagggtgct gagatctga 
        ccgaaaacc gcttccacc aatccacag tgagaacgg 
        gatcgtctt caccgacga ggagaaagc agaagcgtt 
        cgcgttgag catgagcag acaatgctc actcaacct 
        cacgaatgc ggacctgga ccacgtcga agagatcga 
        ggatcacgt ggaatccat cgccaccga agacccaga 
        tgaacccct gaccccgac aacccccga agaagtttc 
        cggagtgat tcggaaact gaagaaacg gaaggcatc 
        cggaccaga cgaaatcag caacagggc tctcaagaa 
        cctcccgct gaaagtcat cgtcgaact gacaggtat 
        tttaaatgc aatgttcag tttcagata ttttccaca 
        gcgttggaa aatggcaac ggtcatttt catcccgaa 
        accggggaa ggacccaaa gtttcccca gaaccacag 
        gccaatcag cttgctgtc ggccgtggg aaaggtcgc 
        agaacgtct catccgcac ccgcctcct ccagctcac 
        ccaggagcg gcacatcgt ccccgatga acaattcgg 
        ctttcggtc gaatcattc aacaactga tcagctact 
        acgagtcgt agagcacgc ttccatcag tatcgagcg 
        caagcaagt tactggtgc agtgtttct cgacgtggc 
        taaagcatt cgatgcggt ttggcatga tgggcttat 
        tcataagct ccatcagac tggcattcc gctcgcaat 
        ggttcaaat gatcagatc gtttctcgt tggacgccg 
        tttccaggt gagaatcaa taactctgt ttcagatcc 
        ccaggacct ggaagccgg agtccctca gggctccgt 
        tctttcacc tctccttta ctccatttt cacccacga 
        catcccaaa aactgaccg tacgacact ggcaatata 
        cgcagatga cacagcgat tctcaccag atcgaagca 
        accctacat ggctacccg ctacttgca ggagtctgt 
        ggaatgcat cgaaaactg gtgtcgcag gtggctcat 
        aaacgtcaa ccccgacaa aagccgagc gcttttgtt 
        ggcacgacg tagagtcag ccctgatgg gtttgttcg 
        aatgttcaa cgcggatat tccctggtc tgaccaggt 
        caaatatct cggggtcat tctggacaa aaaactctc 
        cttcggccc acatcttga ctacgcact cgcgaaggg 
        caagatggc cacgggaat gctgagatc tctcgtatg 
        tcggcggag cgcgttgtc aattgacaa caagctctt 
        gttgtacaa atcagtgat ccgacctac catgactta 
        tgcttctgt ggcttgggc attcgcgcc ctgtaagac 
        tcggatgca taagttaca aactttcca aaacaaatt 
        tctccgtca agcattcaa cgccccgtg gtttgttcg 
        caacaacca attgcaccg agaggtgaa gatgccgac 
        gatggagga attcttccg tgagaccgc aagcga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY