Status
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Gene information
General information
TC005191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451085.1: 1035..6215
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005191
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03917

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005191 RA
      MSTSAQYLI NDPKYSFLK DLGLKASNL GVYDGTWRG 
      SGSLVQSIC PSNGKVIAE VRQGTAEDY TSCVTASQS 
      AWSTWADLP APQRGEIVR QIGDALRAK LHPLGQLVS 
      LEMGKILPE GIGEVQEYV DICDYAVGL SRTLAGAIY 
      PSERPGHVL MEKWNPLGV VGVISAFNF PIAVYGWNS 
      AIAMVCGDT VLWKGAETT PLVSVATTR IVADVLERN 
      HLPGAVAAL CCGGADVGK AMAADERIK LLSFTGSSQ 
      VGHQVGVEV QKRFGRHLL ELGGNNALI VAEDADLNM 
      VVQATLFAC VGTAGQRCT TTRRLILHK NVYDEVLQR 
      LKKAYEQVL HRIGDALDE KTLIGPLHS QTSLQNYEN 
      RIAEIKKQG GTIEFGGKR IEGPGFFVQ PTIVTGLKH 
      DNPLVHSEC FAPIVYILK TESVDEAIS WNNEVPQGL 
      SSSIFTQSI GNIFKWIGP KGSDCGIVN VNIPTSGAE 
      IGGAFGGEK HTGGGRESG SDAWKQYMR RSTITINHS 
      KELPLAQGI KFE
    CDS sequence
    • >TC005191 RA
      atgtccact tcggcccaa tacctcata aacgacccc 
      aaatactca tttctgaag gacttgggc ttgaaggcc 
      tccaacttg ggcgtctac gacggcacg tggaggggc 
      tcaggctcg ttagtccag tcgatttgt cccagcaat 
      ggtaaagtg atcgccgaa gtccgccaa ggcacagcc 
      gaggactac acctcttgc gtcacagcc tcccaatca 
      gcctggagc acgtgggcc gacctccca gccccccag 
      cgcggggaa attgtgcgc cagatcggg gacgccctc 
      cgggccaaa ctccacccc ctggggcag ctcgtttcc 
      ctcgaaatg ggcaaaatc ctccccgag ggaatcggg 
      gaagtccag gagtacgtg gacatctgt gactacgcc 
      gtcggtctc tcgcgcact ctggcaggc gccatctac 
      ccctcggag cgcccaggc cacgtgctg atggaaaag 
      tggaaccct ctgggtgtc gtgggggtg atttcggcc 
      ttcaatttc cccatcgct gtctatggc tggaacagc 
      gccatcgcc atggtctgc ggggacacg gtgctgtgg 
      aagggggcg gaaaccacc cccttggtg tcggtcgcc 
      accacgagg atcgtggcc gacgtcctc gagcgcaac 
      cacctcccg ggggccgtc gcggcgctg tgctgtgga 
      ggcgccgat gtgggcaag gctatggcc gccgacgag 
      aggattaag ctgttgtcg ttcactggg agttcgcag 
      gttgggcac caagtcggg gtggaggtg cagaagagg 
      ttcgggaga catttgctg gagttgggg ggaaataat 
      gcacttatt gttgccgaa gacgctgat ttgaatatg 
      gtggtgcaa gcgacgttg tttgcttgt gtggggacg 
      gcggggcaa aggtgtacc accacgagg aggcttatt 
      ttgcacaaa aatgtctat gacgaggtt ctccaaagg 
      ctgaaaaaa gcctacgag caagtcttg catcgaatt 
      ggggacgca ctggatgaa aaaacctta atagggcca 
      ttacacagt caaacctcc ctacaaaac tatgaaaac 
      agaatagct gagattaaa aagcaaggc gggacgatt 
      gaattcggg ggcaaacga atcgaaggt cctggcttt 
      ttcgtacaa ccaaccatt gtcacaggc ctcaaacac 
      gataatccc ctagttcac agcgaatgt ttcgcgcct 
      attgtctat attttgaaa acggaaagt gtagatgaa 
      gccatttcg tggaataat gaagttccc caaggccta 
      tcgtcaagt attttcaca caaagcatt ggaaatatt 
      ttcaagtgg attggaccc aaaggttcg gactgtggc 
      attgtgaac gtgaatatt cccacttcg ggggctgaa 
      attgggggg gccttcggt ggggaaaaa cacactggt 
      ggggggagg gaatctgga agtgatgcc tggaaacag 
      tacatgagg aggtccact atcacgatc aatcattcc 
      aaggaattg cccttagca caaggaatt aaatttgaa 
      taa
    mRNA sequence
    • >TC005191 RA
      gttattttc cctgtgcaa aaaatttgc tagtgtcca 
      caggatgtc cacttcggc ccaatacct cataaacga 
      ccccaaata ctcatttct gaaggactt gggcttgaa 
      ggcctccaa cttgggcgt ctacgacgg cacgtggag 
      gggctcagg ctcgttagt ccagtcgat ttgtcccag 
      caatggtaa agtgatcgc cgaagtccg ccaaggcac 
      agccgagga ctacacctc ttgcgtcac agcctccca 
      atcagcctg gagcacgtg ggccgacct cccagcccc 
      ccagcgcgg ggaaattgt gcgccagat cggggacgc 
      cctccgggc caaactcca ccccctggg gcagctcgt 
      ttccctcga aatgggcaa aatcctccc cgagggaat 
      cggggaagt ccaggagta cgtggacat ctgtgacta 
      cgccgtcgg tctctcgcg cactctggc aggcgccat 
      ctacccctc ggagcgccc aggccacgt gctgatgga 
      aaagtggaa ccctctggg tgtcgtggg ggtgatttc 
      ggccttcaa tttccccat cgctgtcta tggctggaa 
      cagcgccat cgccatggt ctgcgggga cacggtgct 
      gtggaaggg ggcggaaac caccccctt ggtgtcggt 
      cgccaccac gaggatcgt ggccgacgt cctcgagcg 
      caaccacct cccgggggc cgtcgcggc gctgtgctg 
      tggaggcgc cgatgtggg caaggctat ggccgccga 
      cgagaggat taagctgtt gtcgttcac tgggagttc 
      gcaggttgg gcaccaagt cggggtgga ggtgcagaa 
      gaggttcgg gagacattt gctggagtt ggggggaaa 
      taatgcact tattgttgc cgaagacgc tgatttgaa 
      tatggtggt gcaagcgac gttgtttgc ttgtgtggg 
      gacggcggg gcaaaggtg taccaccac gaggaggct 
      tattttgca caaaaatgt ctatgacga ggttctcca 
      aaggctgaa aaaagccta cgagcaagt cttgcatcg 
      aattgggga cgcactgga tgaaaaaac cttaatagg 
      gccattaca cagtcaaac ctccctaca aaactatga 
      aaacagaat agctgagat taaaaagca aggcgggac 
      gattgaatt cgggggcaa acgaatcga aggtcctgg 
      ctttttcgt acaaccaac cattgtcac aggcctcaa 
      acacgataa tcccctagt tcacagcga atgtttcgc 
      gcctattgt ctatatttt gaaaacgga aagtgtaga 
      tgaagccat ttcgtggaa taatgaagt tccccaagg 
      cctatcgtc aagtatttt cacacaaag cattggaaa 
      tattttcaa gtggattgg acccaaagg ttcggactg 
      tggcattgt gaacgtgaa tattcccac ttcgggggc 
      tgaaattgg gggggcctt cggtgggga aaaacacac 
      tggtggggg gagggaatc tggaagtga tgcctggaa 
      acagtacat gaggaggtc cactatcac gatcaatca 
      ttccaagga attgccctt agcacaagg aattaaatt 
      tgaataaac gccaaaatt gtgattttt ataaataaa 
      tcatcaaaa acttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY