Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
Usually 10 injected animals
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
number of eggs/embryos/larvae on slide: > 50
abdomen musculature specific subset lateral not present -
Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen musculature specific subset lateral orientation irregular -
Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax musculature specific subset partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax musculature thinner -
Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen musculature specific subset lateral thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature rounded -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
| Comment: wt larvae have two oblique lateral muscles. in larvae showing the phenotype one or both oblique muslces were missing. if remaining, these muscles showed a wrong orientation and were thinner than in wt larvae. leg musculature seems to be unaffected. |
larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax musculature specific subset partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
| Comment: the oblique thoracic musculature was nt present. remaining longitudinal thoracic musculature was thinner than normal. penetrance of this phenotype is not sure (not able to see thoracic muscles when larvae is lying on the dorsal side). |
abdomen musculature specific subset lateral thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: larvae showing decreased muscle fluorescence have also thinner muscles, and the oblique lateral muscles are also missing. |
larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
number of eggs/embryos/larvae on slide: > 50
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
gnathal appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
gnathal appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
abdominal odoriferous glands completestructure necrotic -
Number of animals showing the phenotype: 3
(22 dpi)
egg production: number <50% of expected -
ovary without detectable defects -
Number of animals showing the phenotype: 3
| Comment: No phenotype found! |
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