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Gene information
General information
TC005167 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4610453..4618961
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005167
    iBeetle screen iB_03913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature specific subset lateral not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: wt larvae have two oblique lateral muscles. in larvae showing the phenotype one or both oblique muslces were missing. if remaining, these muscles showed a wrong orientation and were thinner than in wt larvae. leg musculature seems to be unaffected.
    abdomen musculature specific subset lateral orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax musculature specific subset partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: the oblique thoracic musculature was nt present. remaining longitudinal thoracic musculature was thinner than normal. penetrance of this phenotype is not sure (not able to see thoracic muscles when larvae is lying on the dorsal side).  
    thorax musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen musculature specific subset lateral thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: larvae showing decreased muscle fluorescence have also thinner muscles, and the oblique lateral muscles are also missing.  
    larval musculature rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially randomnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages random remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands completestructure necrotic - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection



    Fertility
    egg production: number <50% of expected -

    Comment: No phenotype found!
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005167 RA
      MAGIEVDIN SLLAQKDAI IREQRDQII RLKRELDGV 
      ASERDLLLC ENSNLRFEL EMVELKRLQ DESFPPKME 
      RSQIVNSMG SSDQFQEQT YIQAPSFVQ AQNYSTPTP 
      SPIYSRASP NNSTYVNVS QPLHKKGSL RNGDVLKRS 
      RVQTTYELS QDILDKQIE VLERKYGGV KARNAALTI 
      QRAFRRYTL LKKFAAITA MAKAEKRIS RRIPANSED 
      CRSTIGETE QCPEYMRNY EGHDCSNMR VLPVRSMSL 
      RERRNIDNM PIPRSQSGT PTVCWENYS SSSLQHYYS 
      PADSKSDSL GTQVTASPS SCSTPTTST NGYARSRHS 
      LNSRKVPPE VPKRTSSIT SRSVGDPRH HKSNGLTKT 
      NENGSLSSV QSSGSDSSI STERVQCEF SGSPVWKRK 
      GNHISEYAE PALETSLGN ISNTSTSTY TLERANEQQ 
      APASYKITE TIRKRQYRV GLNLFNKKP ERGISYLIR 
      RGFLENSPQ GVARFLISR KGLSKQMIG EYLGNLQNP 
      FCMAVLECF ANELDLSGM QVDVALRKF QQHFRMPGE 
      AQKIERLME VFSHRYCQC NVDIVARLR SPDTIFVLA 
      FAIIMLNTD LHTPNIKPE RRMKLEDFI KNLRGIDDC 
      GDIDSDMLT GVYERVKAN EFKPGSDHV TQVMKVQAT 
      IVGKKPNMA LPHRRLVCY CRLYEIPDI YKKERPGVH 
      QREVFLFND LLVITKILS KKKSSVTYT FRNSFPLCG 
      MTVTLFEAA HYPYGIRLS QRVDGKVLV TFNARNEHD 
      RCKFAEDLK EAVSEMDEM ENLRIEAEL ERQKSNRGT 
      RTGTENRDS GVADVEVCS YQCPCSQNQ ALGGSECAE 
      EVGHDTQIK RSALSNSLL DIHEQFAGE KAQRRGSVG 
      SLDSGMSVS FQSTSASTC SRSDKLANK GKPGLHNHQ 
      SFLGGLFNK KQQNNVAVK STEV
    CDS sequence
    • >TC005167 RA
      atggcagga atagaagtc gatataaat tcgttattg 
      gcccaaaaa gatgccatc atacgggaa caacgggac 
      cagataatt cggctaaag agggaactg gatggagtt 
      gcaagcgag cgggatctt ttgctgtgt gaaaacagc 
      aatctgagg ttcgagctg gaaatggtg gaactgaaa 
      cggttgcaa gatgaaagc tttccaccg aaaatggaa 
      aggtcccag atagtgaat tcgatgggt tcatccgac 
      cagtttcaa gaacagact tacatccag gctcccagt 
      tttgtccaa gctcaaaac tacagtact ccaaccccc 
      agtcctatc tattcccgt gcctcccct aacaactcg 
      acgtacgtg aatgtatcg cagcctctt cataagaag 
      ggttcttta cggaacgga gatgtcttg aaaaggtcg 
      agggttcag acaacatac gagctgtcg caagacatt 
      ctcgacaaa caaatcgaa gtgttggaa cggaaatat 
      ggcggtgtt aaagcgcga aatgcagct ctcacgatt 
      caacgggcg ttcagacga tacacacta cttaaaaaa 
      ttcgcagca attacagca atggccaaa gccgagaaa 
      cgtattagt cgccgaatt cctgcaaat tccgaagac 
      tgtcgtagc acaataggt gagactgag cagtgtccc 
      gaatatatg aggaattat gaaggacac gactgctcg 
      aatatgaga gttcttcca gttagatcg atgtctttg 
      cgcgagaga cgtaatatt gacaatatg ccaatacct 
      aggagtcag tcggggact cctacagtg tgttgggag 
      aattattcc tcatcctcc ctccaacac tactattca 
      cccgctgac tccaaatcg gattcgcta ggtacccaa 
      gtgactgca agtcccagt tcttgtagt acccctaca 
      acttccaca aacggctac gctcgcagc cgccacagt 
      ctgaacagt agaaaagta ccaccagaa gtacctaaa 
      cgaacctca agtattacc tctcgttcg gtcggagac 
      ccccgacac cacaaaagc aacggcttg accaaaaca 
      aacgaaaac ggctcgttg tcaagtgtc caaagttcc 
      ggtagtgac agtagtata tcgactgaa cgcgtgcaa 
      tgtgaattt tccggatcg ccagtctgg aagcgcaaa 
      ggaaatcac atttcggaa tacgccgaa ccggcctta 
      gagaccagt ttagggaac atttccaac actagcaca 
      tccacctac acactagaa cgagccaat gaacaacaa 
      gcacccgcc agctataaa ataaccgag actataaga 
      aaacgacaa tacagagta ggacttaac ttatttaat 
      aaaaaacca gaacgaggc atctcctat ctaatccga 
      agagggttc ttggagaac tcgccgcaa ggcgtcgcc 
      agatttctc atctcgaga aaaggactg agtaaacaa 
      atgatcgga gagtattta gggaattta caaaatccg 
      ttttgtatg gctgttttg gaatgcttc gctaatgag 
      ttagatttg tcgggaatg caagtcgat gttgccctt 
      cggaagttc cagcagcat ttccgaatg cctggagaa 
      gcacaaaag attgaaaga cttatggag gtgttctcg 
      catagatat tgccagtgc aatgtcgat atcgtcgcc 
      cggttaaga tcgcctgat acgattttt gtgctcgcc 
      tttgcgata ataatgctg aacactgac ctccacacc 
      ccgaatata aaacccgaa cgtcggatg aagctcgaa 
      gacttcata aaaaattta cgaggtatc gacgattgt 
      ggtgatatt gacagtgat atgttgact ggggtttac 
      gagcgtgtc aaagcgaac gaatttaag cccggttca 
      gaccacgtg actcaagta atgaaagtc caagcaacg 
      atcgttggt aaaaaacca aatatggcg ttaccacat 
      cgccgacta gtgtgttat tgtcgttta tacgaaatc 
      cctgatatt tataagaaa gaaagacct ggtgtacac 
      cagagagaa gtgttcctt tttaacgat ttgctcgtt 
      attacgaaa attctaagt aagaagaag tcttcagtt 
      acgtataca ttcaggaat agtttccct ctgtgcggg 
      atgactgtc actctcttc gaagctgcc cattaccct 
      tatggtata cgtttgagc caacgtgtc gatggcaaa 
      gtattggtc acatttaac gcgcgaaat gagcatgat 
      aggtgcaaa ttcgcggag gatttgaag gaggctgta 
      agcgagatg gatgaaatg gagaatttg aggattgag 
      gctgagcta gagcggcag aagtcgaac cgggggacc 
      aggacaggt accgaaaac cgggacagt ggcgtcgct 
      gacgtggaa gtgtgttct taccagtgc ccctgctcc 
      cagaaccag gcactagga gggtcggag tgtgccgaa 
      gaagtcggt catgacact caaatcaaa cgttcggcg 
      ctcagtaac tcgttgctg gatatccac gaacagttt 
      gcaggagaa aaggcacag cgccgaggt agtgtgggc 
      tcgttagat agcggcatg tctgtatca ttccagtcg 
      acgtcggct agtacttgc tcccggtct gacaagctg 
      gctaataaa ggtaagcca ggcctccat aaccaccag 
      tcattctta ggcggcctg ttcaacaag aaacaacag 
      aacaatgtg gcggtgaag tcgaccgaa gtgtaa
    mRNA sequence
    • >TC005167 RA
      taagacaag tcgctaagt taccatcct ggaagtgtt 
      actggaggg gatttccag atggggcaa cgtgtgtag 
      gtttgttca tcccgtttt gttctgtgc tctcgctcc 
      tgacgtaag agagacaga tgaataata gtgagaaaa 
      accaaggaa cagtgcggc gcgttgtat ttcgtctgt 
      caatatcgt ttcagaata agatgtgat aattttttt 
      gaaaaactt gtgtgaaaa aaggaaaaa ccaaatcgt 
      tgctggagg aaaatgccc gtcagatta aaaatttgg 
      acggtaccg taacaactg aggatcgtg tgtgtgtga 
      acagtagga gacctgcaa tggcaggaa tagaagtcg 
      atataaatt cgttattgg cccaaaaag atgccatca 
      tacgggaac aacgggacc agataattc ggctaaaga 
      gggaactgg atggagttg caagcgagc gggatcttt 
      tgctgtgtg aaaacagca atctgaggt tcgagctgg 
      aaatggtgg aactgaaac ggttgcaag atgaaagct 
      ttccaccga aaatggaaa ggtcccaga tagtgaatt 
      cgatgggtt catccgacc agtttcaag aacagactt 
      acatccagg ctcccagtt ttgtccaag ctcaaaact 
      acagtactc caaccccca gtcctatct attcccgtg 
      cctccccta acaactcga cgtacgtga atgtatcgc 
      agcctcttc ataagaagg gttctttac ggaacggag 
      atgtcttga aaaggtcga gggttcaga caacatacg 
      agctgtcgc aagacattc tcgacaaac aaatcgaag 
      tgttggaac ggaaatatg gcggtgtta aagcgcgaa 
      atgcagctc tcacgattc aacgggcgt tcagacgat 
      acacactac ttaaaaaat tcgcagcaa ttacagcaa 
      tggccaaag ccgagaaac gtattagtc gccgaattc 
      ctgcaaatt ccgaagact gtcgtagca caataggtg 
      agactgagc agtgtcccg aatatatga ggaattatg 
      aaggacacg actgctcga atatgagag ttcttccag 
      ttagatcga tgtctttgc gcgagagac gtaatattg 
      acaatatgc caataccta ggagtcagt cggggactc 
      ctacagtgt gttgggaga attattcct catcctccc 
      tccaacact actattcac ccgctgact ccaaatcgg 
      attcgctag gtacccaag tgactgcaa gtcccagtt 
      cttgtagta cccctacaa cttccacaa acggctacg 
      ctcgcagcc gccacagtc tgaacagta gaaaagtac 
      caccagaag tacctaaac gaacctcaa gtattacct 
      ctcgttcgg tcggagacc cccgacacc acaaaagca 
      acggcttga ccaaaacaa acgaaaacg gctcgttgt 
      caagtgtcc aaagttccg gtagtgaca gtagtatat 
      cgactgaac gcgtgcaat gtgaatttt ccggatcgc 
      cagtctgga agcgcaaag gaaatcaca tttcggaat 
      acgccgaac cggccttag agaccagtt tagggaaca 
      tttccaaca ctagcacat ccacctaca cactagaac 
      gagccaatg aacaacaag cacccgcca gctataaaa 
      taaccgaga ctataagaa aacgacaat acagagtag 
      gacttaact tatttaata aaaaaccag aacgaggca 
      tctcctatc taatccgaa gagggttct tggagaact 
      cgccgcaag gcgtcgcca gatttctca tctcgagaa 
      aaggactga gtaaacaaa tgatcggag agtatttag 
      ggaatttac aaaatccgt tttgtatgg ctgttttgg 
      aatgcttcg ctaatgagt tagatttgt cgggaatgc 
      aagtcgatg ttgcccttc ggaagttcc agcagcatt 
      tccgaatgc ctggagaag cacaaaaga ttgaaagac 
      ttatggagg tgttctcgc atagatatt gccagtgca 
      atgtcgata tcgtcgccc ggttaagat cgcctgata 
      cgatttttg tgctcgcct ttgcgataa taatgctga 
      acactgacc tccacaccc cgaatataa aacccgaac 
      gtcggatga agctcgaag acttcataa aaaatttac 
      gaggtatcg acgattgtg gtgatattg acagtgata 
      tgttgactg gggtttacg agcgtgtca aagcgaacg 
      aatttaagc ccggttcag accacgtga ctcaagtaa 
      tgaaagtcc aagcaacga tcgttggta aaaaaccaa 
      atatggcgt taccacatc gccgactag tgtgttatt 
      gtcgtttat acgaaatcc ctgatattt ataagaaag 
      aaagacctg gtgtacacc agagagaag tgttccttt 
      ttaacgatt tgctcgtta ttacgaaaa ttctaagta 
      agaagaagt cttcagtta cgtatacat tcaggaata 
      gtttccctc tgtgcggga tgactgtca ctctcttcg 
      aagctgccc attaccctt atggtatac gtttgagcc 
      aacgtgtcg atggcaaag tattggtca catttaacg 
      cgcgaaatg agcatgata ggtgcaaat tcgcggagg 
      atttgaagg aggctgtaa gcgagatgg atgaaatgg 
      agaatttga ggattgagg ctgagctag agcggcaga 
      agtcgaacc gggggacca ggacaggta ccgaaaacc 
      gggacagtg gcgtcgctg acgtggaag tgtgttctt 
      accagtgcc cctgctccc agaaccagg cactaggag 
      ggtcggagt gtgccgaag aagtcggtc atgacactc 
      aaatcaaac gttcggcgc tcagtaact cgttgctgg 
      atatccacg aacagtttg caggagaaa aggcacagc 
      gccgaggta gtgtgggct cgttagata gcggcatgt 
      ctgtatcat tccagtcga cgtcggcta gtacttgct 
      cccggtctg acaagctgg ctaataaag gtaagccag 
      gcctccata accaccagt cattcttag gcggcctgt 
      tcaacaaga aacaacaga acaatgtgg cggtgaagt 
      cgaccgaag tgtaaatca atccaaata tataagtgt 
      aatcggttc tttttcaaa tttaacttg tgcatgtat 
      gaataacca aatacattt tcaccattt ataagtgtt 
      accaaatat attaattat ggctgtcta atttcgccc 
      cagcgcgta atacttaat tttttgtta ttattattt 
      tttaatttc cttcttgtt actaggaga ggcaaactt 
      aatgtattt tctttacca ttatattat attcactcg 
      ctattttga cagctattc aacattaag gttgcctta 
      aaccggctc tctgtcttc aatggcatt gaacatatt 
      gataattgt cttctaatg ttgcctgta aaggatttg 
      taaatagtt aaattatta aagagagca acatgttct 
      agtcgattg taaatatgt tagcaatct ctactaatg 
      atattaaat aatttaaat gtatgtaat aagtgtttt 
      tttaataac caataaata gtcggacct tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY