Status
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Gene information
General information
TC033284
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005157,TC005158
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451657.1: 21185..28369
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033284
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03912

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033284 RA
      MLSSKFNSI SLNIEGTIV KALIDTGSD ISLVKYSEL 
      NSNQRQRIT ESDLILRDV QGTTIKNIG KVKLTVKIG 
      KTKRAIHQF IAVGNEMRF RGTLLLGLD FLHRFEVFI 
      CWSEKTVKL LNQEIQLET EEDEIRLIE KEDASKKIE 
      ASHLQVVQK EEKEKSLIT NESEGQGDF PGEVEQPDE 
      GKHPQSHTG PKAGRIDPK ETSWKEVVT VMTESILRV 
      IRTLRQGEL ARRIPSSAR RRGIKTEKR KNNAYTMPD 
      NPPPPPPPP GNQLRNALG LSTAATQLI PNYSGEDSV 
      RTFLSYVED VSELEGWNE VQKVKVARL KLQGPARRL 
      VEWNEELKS PACTWIQLK NALIQRFEK KVMPVEQLH 
      RFRSCTQRE GESVTEFAE RLQMLGAAT MKKTADPNR 
      NEWIRAQLL EECLVQFLE GLNTAIQYK VVQKEEKEK 
      SLITNESEG QGDFPGEVE QPDEGKHPQ SHTSPKAGR 
      IDPKETFME GNCNTEDGE HSQSHTDPT AGGIGPQNT 
      VISSQEMNI NRKEGKQCV YVEDGRKKR NEKRNGRRW 
      YHQSG
    CDS sequence
    • >TC033284 RA
      atgttaagt tcaaaattc aattcaatc tcattaaac 
      atcgaagga accatcgta aaagcatta atcgacacc 
      ggctcggac atatcattg gttaaatac tctgaatta 
      aacagcaac caaagacag cgaattacg gagagcgac 
      ttaatcttg cgggatgtt caagggaca acaattaag 
      aatatcgga aaagtgaaa ttaactgtt aaaattggg 
      aaaacgaag agagcaatt catcagttt atagcagta 
      ggcaacgaa atgagattt cggggaaca ttgttactc 
      ggactagac tttttacat agattcgag gttttcatt 
      tgttggtct gaaaagaca gtgaagtta ctgaatcag 
      gaaatccaa ttggagacg gaagaagat gaaataaga 
      ttaatcgaa aaggaagat gcatcaaag aaaattgag 
      gcgtcacac ctgcaggta gttcaaaaa gaagagaaa 
      gaaaaaagc ctgatcact aatgagagt gaaggacaa 
      ggagacttc ccaggggaa gtggaacaa ccagatgaa 
      ggaaagcat cctcagagt catacgggc cctaaggca 
      gggagaatt gacccgaag gagacctca tggaaggaa 
      gttgtaaca gtgatgacg gagagcatt ctcagagtc 
      atacggacc ctacggcag ggggaattg gcccgcaga 
      ataccgtca tcagctcgc aggagagga ataaaaaca 
      gaaaagagg aaaaacaat gcgtatacg atgcctgac 
      aatccacca ccaccacca ccaccacca ggaaaccaa 
      ctaaggaat gcactaggg ttatcaact gctgcgacg 
      caattaata cctaattat tcgggcgaa gactcggta 
      cgcacgttt ttgagctat gtcgaagat gtctcggag 
      ctggaggga tggaacgag gtgcaaaag gtcaaagtg 
      gcacgtctt aaattgcaa ggacccgct cggagatta 
      gttgaatgg aatgaggaa ttgaaaagt cccgcttgc 
      acttggatt caattaaaa aatgcattg attcaaaga 
      tttgagaaa aaggtgatg cctgtagaa caactccat 
      cggttccgc tcatgtact caacgagaa ggggaatct 
      gtgaccgaa tttgcagaa agattgcaa atgttagga 
      gctgcaact atgaagaaa acagctgat ccgaatcga 
      aatgagtgg attagggca caacttcta gaggaatgc 
      ttagtacaa ttcttagag gggttgaac acagcaatt 
      cagtacaaa gtagttcaa aaagaagag aaagaaaaa 
      agcctgatc actaatgag agtgaagga caaggagac 
      ttcccaggg gaagtggaa caaccagat gaaggaaag 
      catcctcag agtcatacg agccctaag gcagggaga 
      attgacccg aaggagacc ttcatggaa ggaaattgt 
      aacactgaa gacggagag cattcccag agtcatacg 
      gaccctacg gcaggggga attggcccg cagaatacc 
      gtcatcagc tcgcaggag atgaatata aacagaaaa 
      gagggaaaa cagtgcgta tacgtagaa gatgggaga 
      aaaaaaaga aatgagaaa agaaacgga aggaggtgg 
      taccaccaa agcggataa
    mRNA sequence
    • >TC033284 RA
      acacaggaa aacatccaa cccagacaa caacgacaa 
      tttgcaaac acatcaaaa tttaacagc aatcaacaa 
      tcagaaaat tttaaatgt tgaggttgc ggtcaaaag 
      ggacacacg cgcgcgaat tgcaaaaat tgtttcgaa 
      tgcggtaaa tccggtcac atgcagcgc gactgtttt 
      atattcaag cgtaaacag gcaggtcaa agaacccag 
      ggtgggcct caaaacaag gcaaattta aacaggaac 
      agggctgtc tccaagaat tggggggca gcccgcaat 
      taaatttaa caaaaacaa gtacaacgt cccaaaacc 
      tcgtttgtc acattatgt taagttcaa aattcaatt 
      caatctcat taaacatcg aaggaacca tcgtaaaag 
      cattaatcg acaccggct cggacatat cattggtta 
      aatactctg aattaaaca gcaaccaaa gacagcgaa 
      ttacggaga gcgacttaa tcttgcggg atgttcaag 
      ggacaacaa ttaagaata tcggaaaag tgaaattaa 
      ctgttaaaa ttgggaaaa cgaagagag caattcatc 
      agtttatag cagtaggca acgaaatga gatttcggg 
      gaacattgt tactcggac tagactttt tacatagat 
      tcgaggttt tcatttgtt ggtctgaaa agacagtga 
      agttactga atcaggaaa tccaattgg agacggaag 
      aagatgaaa taagattaa tcgaaaagg aagatgcat 
      caaagaaaa ttgaggcgt cacacctgc aggtagttc 
      aaaaagaag agaaagaaa aaagcctga tcactaatg 
      agagtgaag gacaaggag acttcccag gggaagtgg 
      aacaaccag atgaaggaa agcatcctc agagtcata 
      cgggcccta aggcaggga gaattgacc cgaaggaga 
      cctcatgga aggaagttg taacagtga tgacggaga 
      gcattctca gagtcatac ggaccctac ggcaggggg 
      aattggccc gcagaatac cgtcatcag ctcgcagga 
      gaggaataa aaacagaaa agaggaaaa acaatgcgt 
      atacgatgc ctgacaatc caccaccac caccaccac 
      caccaggaa accaactaa ggaatgcac tagggttat 
      caactgctg cgacgcaat taataccta attattcgg 
      gcgaagact cggtacgca cgtttttga gctatgtcg 
      aagatgtct cggagctgg agggatgga acgaggtgc 
      aaaaggtca aagtggcac gtcttaaat tgcaaggac 
      ccgctcgga gattagttg aatggaatg aggaattga 
      aaagtcccg cttgcactt ggattcaat taaaaaatg 
      cattgattc aaagatttg agaaaaagg tgatgcctg 
      tagaacaac tccatcggt tccgctcat gtactcaac 
      gagaagggg aatctgtga ccgaatttg cagaaagat 
      tgcaaatgt taggagctg caactatga agaaaacag 
      ctgatccga atcgaaatg agtggatta gggcacaac 
      ttctagagg aatgcttag tacaattct tagaggggt 
      tgaacacag caattcagt acaaagtag ttcaaaaag 
      aagagaaag aaaaaagcc tgatcacta atgagagtg 
      aaggacaag gagacttcc caggggaag tggaacaac 
      cagatgaag gaaagcatc ctcagagtc atacgagcc 
      ctaaggcag ggagaattg acccgaagg agaccttca 
      tggaaggaa attgtaaca ctgaagacg gagagcatt 
      cccagagtc atacggacc ctacggcag ggggaattg 
      gcccgcaga ataccgtca tcagctcgc aggagatga 
      atataaaca gaaaagagg gaaaacagt gcgtatacg 
      tagaagatg ggagaaaaa aaagaaatg agaaaagaa 
      acggaagga ggtggtacc accaaagcg gataacgag 
      gttatcgag ggaacagag gtgtggaac gcgaaatac 
      gta
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