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Gene information
General information
TC005134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 127617..139232
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005134
    iBeetle screen iB_03911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005134 RA
      MLPKLCSTS QIINRLETS LFNKDLGIK FLSTKEIID 
      REKKYAAHN YHPLPVALS RGEGVFVWD VKGKRYFDY 
      LSGYSANNF GHCHPKIVQ TIQKQAQIL HHTSRAFYS 
      DVFGEYAQF VTKLFGYDK VMPMNTGVE ADDTACKIS 
      RKWGYKVKK IPKNEAKMI FAVDNFWGR SLAAVSAST 
      DPSSFEGYG PFMPGFSCV PYNDLIALE KALQDPLVC 
      GFMVEPIQG EAGVVVPSE GYLKGVRNL CTKYNVLWT 
      ADEIQTGLG RTGKRLAVD YEDVKPDIL VLGKALGGG 
      VYPVSACLA NDPIMLVIE PGTHGSTFG GNPLGSKVA 
      IAALEVLEE ENLAQNAFE LGEIFRVQL RKRLSKDVV 
      IEVRGRGLL NAIVINKNY IEAWDVCLK MKENGLIAK 
      HTHDNIIRF APPLIMTKD QLQESIEII VKTINSIET 
      K
    CDS sequence
    • >TC005134 RA
      atgctaccg aaactgtgt tccacctct caaatcata 
      aatcggcta gaaacctcg ttgtttaat aaagattta 
      ggaatcaaa tttctgagt actaaagag atcatcgac 
      agggaaaag aaatacgct gctcacaat tatcatcct 
      ttacctgtg gctttatct agaggagaa ggtgttttt 
      gtatgggac gtcaaggga aaacgatat tttgattat 
      ttgagtggt tactcagcg aataatttc ggacattgt 
      catccaaaa attgttcaa actatacaa aagcaagca 
      caaattttg catcatact tccagggcg ttttactcg 
      gatgttttt ggggaatat gcacagttt gtgactaaa 
      ctatttgga tatgacaaa gttatgcca atgaacaca 
      ggtgtggag gccgacgat accgcttgc aaaatctct 
      cgtaaatgg ggttacaaa gtgaaaaaa atccccaaa 
      aatgaagca aagatgatt ttcgctgtt gataatttc 
      tgggggcgt tctttggct gcggtttcc gcttctacc 
      gatccatcg tctttcgaa ggttatggt ccttttatg 
      ccaggcttc agctgcgtt ccttataac gatctcatt 
      gcgttagag aaagccctt caagaccct ctagtctgc 
      ggatttatg gtcgaaccc attcaaggg gaggccgga 
      gtagttgtc ccaagcgaa ggttaccta aaaggcgtt 
      cgaaatttg tgtacaaaa tataacgtc ctatggacg 
      gccgatgaa atccaaact ggattagga agaacagga 
      aaaagacta gctgttgac tacgaggat gtcaaaccg 
      gatatttta gtcttgggc aaggcttta ggaggtggt 
      gtctatcca gtatctgcg tgtttggca aatgatccc 
      ataatgcta gtcattgaa cccggcacc catggctcc 
      acttttggt gggaatccc ctaggttcc aaagtagcg 
      atagcagcc ttagaagta ttagaagaa gagaattta 
      gctcagaat gcatttgaa cttggggaa attttccga 
      gttcagttg cgaaaacgg ttaagcaag gatgttgta 
      attgaagtg agaggaaga ggattactc aatgcgata 
      gtcataaat aaaaattat atcgaagct tgggatgtc 
      tgtctgaaa atgaaagaa aacggtctg atagcgaag 
      catactcat gacaacatc atcagattt gcgcctccg 
      cttataatg actaaagat caactgcaa gaatcaatt 
      gaaattatt gttaaaaca attaacagt attgaaaca 
      aagtaa
    mRNA sequence
    • >TC005134 RA
      ggggtgtag ttttgggta gtttttagt taagtttta 
      gttcataag atgactatg taagaaaca gtcagaaca 
      agtaaacgt ttcttccaa caaaattca aaatcttcg 
      tttctattt gggctctgg tccttcgac tacctttac 
      agttttgtt tataacctt tgtgtttgt acagagttt 
      tgaaaataa ttatgggag tgctttgta actggttaa 
      caatttaat tttgttgtc cttaaaatt ttatttaaa 
      acgtcgtaa actcttctc tcttcacac atgtgctgc 
      gtttttact tccagttta tagaacatt gcatttata 
      cagtaagcc gatactttc tcttattaa aactagctc 
      ctgtgcatt tagatgatt ttgacaaaa atgctaccg 
      aaactgtgt tccacctct caaatcata aatcggcta 
      gaaacctcg ttgtttaat aaagattta ggaatcaaa 
      tttctgagt actaaagag atcatcgac agggaaaag 
      aaatacgct gctcacaat tatcatcct ttacctgtg 
      gctttatct agaggagaa ggtgttttt gtatgggac 
      gtcaaggga aaacgatat tttgattat ttgagtggt 
      tactcagcg aataatttc ggacattgt catccaaaa 
      attgttcaa actatacaa aagcaagca caaattttg 
      catcatact tccagggcg ttttactcg gatgttttt 
      ggggaatat gcacagttt gtgactaaa ctatttgga 
      tatgacaaa gttatgcca atgaacaca ggtgtggag 
      gccgacgat accgcttgc aaaatctct cgtaaatgg 
      ggttacaaa gtgaaaaaa atccccaaa aatgaagca 
      aagatgatt ttcgctgtt gataatttc tgggggcgt 
      tctttggct gcggtttcc gcttctacc gatccatcg 
      tctttcgaa ggttatggt ccttttatg ccaggcttc 
      agctgcgtt ccttataac gatctcatt gcgttagag 
      aaagccctt caagaccct ctagtctgc ggatttatg 
      gtcgaaccc attcaaggg gaggccgga gtagttgtc 
      ccaagcgaa ggttaccta aaaggcgtt cgaaatttg 
      tgtacaaaa tataacgtc ctatggacg gccgatgaa 
      atccaaact ggattagga agaacagga aaaagacta 
      gctgttgac tacgaggat gtcaaaccg gatatttta 
      gtcttgggc aaggcttta ggaggtggt gtctatcca 
      gtatctgcg tgtttggca aatgatccc ataatgcta 
      gtcattgaa cccggcacc catggctcc acttttggt 
      gggaatccc ctaggttcc aaagtagcg atagcagcc 
      ttagaagta ttagaagaa gagaattta gctcagaat 
      gcatttgaa cttggggaa attttccga gttcagttg 
      cgaaaacgg ttaagcaag gatgttgta attgaagtg 
      agaggaaga ggattactc aatgcgata gtcataaat 
      aaaaattat atcgaagct tgggatgtc tgtctgaaa 
      atgaaagaa aacggtctg atagcgaag catactcat 
      gacaacatc atcagattt gcgcctccg cttataatg 
      actaaagat caactgcaa gaatcaatt gaaattatt 
      gttaaaaca attaacagt attgaaaca aagtaaatg 
      ttttgttag actttatta tttttgtat tatcatcat 
      tttcttaat cgtgtaatt ttacaaaaa taaatgttt 
      gttttttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY