Status
Please wait ...
Gene information
General information
TC005133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid104: 114558..116752
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005133
    iBeetle screen iB_03910
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005133 RA
      MTKACLFVI FTLFQAGFL LGVPEVQLP TGVIRGRED 
      TTVNNKQYF AFEKVPYAA PPIGVLRFK APVPPPNWE 
      EPLDTVNLD VSCYQVSGN SDAESEDCL YINVFTPQL 
      PSGTTNVSL PVMLFIHGG GFMFGSGRN TYSDYIIEE 
      NVIFATINY RLGPFGFLS TEDHYIPGN NGLKDQHMA 
      IKWTHSNIH LFGGDPDKI TIMGQSAGS ASVAYHLLN 
      QQSQGLFQS AILESGSFL SPFAFQRRA REIAFATAA 
      FLNDTFDTT ENSKELYEF LKGVDAKDL DLASQKYHE 
      SETLESAQI LTGRYYSPV IEVKNPDAF LTKKMYGLL 
      KAGNLVRVP ILIGINSEE GLVYNTNPD ILTQNMATF 
      DENVSWLVP KDMMLIDDQ PLLEMGTQI KTMYVGSGN 
      LSEHIGDGV RYYSDHSFT RSIIKTAEL YSNFSETFF 
      YQFSYDGPL GGVNIHYDG AENVGHAEE LSYLFCHGS 
      GCNRDDIPD SDKLTRQRL IKLWTNFAK YQNPTPQPE 
      ELLQNVTWP KVSTESGDF LYLDIDENL EVKNHPKEE 
      TYSKWVELY ESLGYDNFD TY
    CDS sequence
    • >TC005133 RA
      atgacaaaa gcatgtttg tttgtaatt ttcacacta 
      ttccaagcc ggttttttg cttggggtc cctgaggtc 
      caactcccg actggagtg atcaggggc cgtgaagac 
      acaacagtt aacaataaa caatacttt gcttttgaa 
      aaagttcca tatgcggct cccccaatt ggagtcttg 
      cgttttaaa gccccggta ccacctcca aattgggag 
      gaaccactt gatacagtc aatttggat gtttcttgt 
      taccaagtt agcggaaat tcagacgct gagtctgag 
      gattgtctt tatatcaat gtttttaca ccacagctt 
      ccaagtggt accaccaat gttagttta ccagttatg 
      ttatttata catggtggg gggtttatg tttggttct 
      ggtcgaaac acttattcg gattatatc attgaggaa 
      aatgtgatt tttgcaaca attaactac agattggga 
      ccatttggt ttcctatca actgaggac cactatatt 
      ccgggaaac aatggtctt aaggaccag cacatggct 
      ataaaatgg acccatagc aacatacat ttattcgga 
      ggtgaccct gataagata acaataatg ggtcaaagt 
      gccgggagt gcttctgtt gcctatcac ttactaaat 
      caacaatca caaggattg tttcaaagc gctattttg 
      gaaagtggt tcgtttttg agccctttt gcgttccaa 
      agacgcgca agagaaatt gcgtttgcc accgctgct 
      tttctcaac gatactttt gacacaaca gagaattcg 
      aaagaattg tacgaattt ttgaaagga gttgacgcg 
      aaagattta gatctggct tcacagaag taccatgaa 
      tcggaaaca cttgaaagt gcccaaatt ttgactgga 
      aggtattat tcaccggtg attgaagta aaaaatccg 
      gacgctttc cttacgaaa aaaatgtac ggtttgttg 
      aaggctgga aatttggtg cgagtacca attttgatt 
      gggattaac tcggaggaa ggactcgtg tacaatact 
      aatccggat attttaaca caaaacatg gcgactttt 
      gacgaaaat gtgtcttgg ttggtaccc aaagacatg 
      atgctgatt gatgaccaa ccccttcta gagatggga 
      acccaaatt aaaacaatg tatgttggt tctggcaat 
      ttgagtgag catatcggt gacggcgtt cgctattac 
      agtgaccac tcttttacc cgatctatc atcaaaacc 
      gccgaactt tactccaac ttttctgaa actttcttt 
      taccaattt tcctacgat ggccctctt ggaggtgta 
      aacatccat tacgatggg gctgaaaat gtcggtcac 
      gccgaagaa ctgagttat ttattttgt catggttct 
      ggctgcaat cgtgacgat attcctgat tcggataaa 
      ttaactcgt caaagattg attaaactt tggacgaat 
      tttgcaaaa tatcagaat ccgacaccg caacctgaa 
      gaactctta caaaatgtt acttggcct aaagtttcg 
      actgagagt ggcgatttt ttgtatcta gatatcgac 
      gaaaatttg gaggtaaaa aatcacccg aaggaggaa 
      acttattcg aaatgggtg gagctctat gaaagtctt 
      ggatatgat aattttgat acatattaa
    mRNA sequence
    • >TC005133 RA
      tgataatca atttattat cacacaatg catcaattt 
      cgtttaaaa ataatcaaa acactcatt caacttgag 
      actatagca acatgacaa aagcatgtt tgtttgtaa 
      ttttcacac tattccaag ccggttttt tgcttgggg 
      tccctgagg tccaactcc cgactggag tgatcaggg 
      gccgtgaag acacaacag ttaacaata aacaatact 
      ttgcttttg aaaaagttc catatgcgg ctcccccaa 
      ttggagtct tgcgtttta aagccccgg taccacctc 
      caaattggg aggaaccac ttgatacag tcaatttgg 
      atgtttctt gttaccaag ttagcggaa attcagacg 
      ctgagtctg aggattgtc tttatatca atgttttta 
      caccacagc ttccaagtg gtaccacca atgttagtt 
      taccagtta tgttattta tacatggtg gggggttta 
      tgtttggtt ctggtcgaa acacttatt cggattata 
      tcattgagg aaaatgtga tttttgcaa caattaact 
      acagattgg gaccatttg gtttcctat caactgagg 
      accactata ttccgggaa acaatggtc ttaaggacc 
      agcacatgg ctataaaat ggacccata gcaacatac 
      atttattcg gaggtgacc ctgataaga taacaataa 
      tgggtcaaa gtgccggga gtgcttctg ttgcctatc 
      acttactaa atcaacaat cacaaggat tgtttcaaa 
      gcgctattt tggaaagtg gttcgtttt tgagccctt 
      ttgcgttcc aaagacgcg caagagaaa ttgcgtttg 
      ccaccgctg cttttctca acgatactt ttgacacaa 
      cagagaatt cgaaagaat tgtacgaat ttttgaaag 
      gagttgacg cgaaagatt tagatctgg cttcacaga 
      agtaccatg aatcggaaa cacttgaaa gtgcccaaa 
      ttttgactg gaaggtatt attcaccgg tgattgaag 
      taaaaaatc cggacgctt tccttacga aaaaaatgt 
      acggtttgt tgaaggctg gaaatttgg tgcgagtac 
      caattttga ttgggatta actcggagg aaggactcg 
      tgtacaata ctaatccgg atattttaa cacaaaaca 
      tggcgactt ttgacgaaa atgtgtctt ggttggtac 
      ccaaagaca tgatgctga ttgatgacc aaccccttc 
      tagagatgg gaacccaaa ttaaaacaa tgtatgttg 
      gttctggca atttgagtg agcatatcg gtgacggcg 
      ttcgctatt acagtgacc actctttta cccgatcta 
      tcatcaaaa ccgccgaac tttactcca acttttctg 
      aaactttct tttaccaat tttcctacg atggccctc 
      ttggaggtg taaacatcc attacgatg gggctgaaa 
      atgtcggtc acgccgaag aactgagtt atttatttt 
      gtcatggtt ctggctgca atcgtgacg atattcctg 
      attcggata aattaactc gtcaaagat tgattaaac 
      tttggacga attttgcaa aatatcaga atccgacac 
      cgcaacctg aagaactct tacaaaatg ttacttggc 
      ctaaagttt cgactgaga gtggcgatt ttttgtatc 
      tagatatcg acgaaaatt tggaggtaa aaaatcacc 
      cgaaggagg aaacttatt cgaaatggg tggagctct 
      atgaaagtc ttggatatg ataattttg atacatatt 
      aagtgtaat taaactttt atagtaaaa ataaacatg 
      ttacccggc g