General information | |||
TC005107 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450592.1: 6175..10351 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC005107 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03907 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
egg chamber potentially fused - Number of animals showing the phenotype: 3
tropharium potentially shape irregular - Number of animals showing the phenotype: 3
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC005107 RA
MCDIKRRLS QMDDFNEKM FEGKPWAEK LLSESIKLT KKAVANWCH IVCKIGEAE VDLDDYVTI FLQQIETLY EDLIADVDT IHEKSLDKI MKLLEKLEN LCRDLHVPI PTFNYEGVP LSEVRLQIV QKVDEFEAL AQIRRREFS ELQQKELKL CACLGRAPR LLPGAPLPL PPELEELKA YVENLETEA MEREEVFFQ LKEKIVEIV SELEMCPNL EFEKDILNL SNTQVRITD AEMEQLRQF HDQLVKRLA DVTEEVEEL RSRIDDLWK KLEVDLVER DDFRARHVG HSLKTLEAL KEELNRLKA MKKAKLQSF ILKQRDELE EWWEKCHCT QKQRDDFTF YYSDCFTED LFDIHEREI EKWKNYHEE NSEIFALLK EFKTLWTEQ IELESNSKG ANRYKNRGG QLLIEERKR NKLKKRLPV VEDKLRSLA QAYLVRNGQ EFETFGQTI DDYIQQLHE NKERDFKQK LSAKKQLRE QQPSCSSRL PTTPRHCSK RKVTATPAT EPSKKAKLA VLKKVSKTG LPKFNTSKT KNNRRRSSA HKQRLRSRL FKSSDKENQ SNVSTTYHD FEKEISARD ACRSTIGGT IGLQVTNPE GIRIKQENF AIPSGIAPL PRTPRPKNP SKF
- >TC005107 RA
atgtgcgac ataaagcgc cggctgtct caaatggac gatttcaac gaaaaaatg tttgagggc aagccgtgg gccgaaaaa ctgttatct gaatcgata aaattgaca aaaaaggca gtggcgaac tggtgccac atcgtgtgt aaaatcggc gaagccgag gtagacctg gacgattat gtgacaatt ttcctccaa caaatcgaa accctttac gaagacctg atcgccgat gtggacaca atccacgag aaaagtctc gataaaata atgaaactg ttggagaaa ctcgaaaat ctctgtcgc gacctccac gtcccaatc cccactttc aactacgaa ggggttcct ctgagtgag gtgcgcctc caaatcgtc caaaaggtg gacgaattc gaggctttg gcccagatt cgccgacgg gagtttagc gagttgcag cagaaagag ctcaaactg tgtgcctgt ctggggcgg gccccccga ctgctcccc ggggcccca ctcccgttg ccccccgag ctggaggag ttgaaggcc tatgtcgag aacctggag acggaggcg atggaacgg gaggaggtt ttcttccaa ctgaaggaa aaaatcgtc gagattgtc tccgaacta gagatgtgc cctaatcta gagttcgag aaggatatt ttgaatttg tccaatacc caagtgcgc ataaccgac gcagaaatg gagcagttg cggcagttc cacgaccag ttggtcaag cgcctcgct gacgtgacc gaggaggtt gaagagttg aggagcagg atcgacgat ttatggaaa aaattggag gttgatttg gtggagcgt gatgatttc cgggcgaga cacgtgggg cactcgttg aagacgctg gaggcgctt aaagaggag ttgaatagg ttgaaggcg atgaaaaag gccaagctt cagagtttc attttaaag caacgagat gagctcgaa gaatggtgg gaaaagtgt cactgtaca caaaaacaa cgcgatgat tttacgttt tactactcc gattgtttt accgaagat ttgttcgat atacatgag cgtgaaatc gagaaatgg aagaattat cacgaggag aactctgaa atctttgca cttctcaag gagtttaag acgttgtgg acggaacag atcgagcta gaatcgaat tctaaaggt gctaatcgg tacaagaac cgcggaggg caattgttg attgaggaa aggaaacgt aataagctc aaaaaacga cttccagta gttgaggat aagctccga agtttggct caagcgtat ttggtccga aatgggcaa gaattcgag acgtttggg caaacaatc gacgactac attcaacaa ctgcacgaa aataaagaa cgggatttt aaacagaaa ctgagtgcc aagaaacaa ctgcgtgag caacagccg tcctgttcg agcagactg cccacaact ccacgccac tgctccaaa cgcaaagtc acagcgact ccagcgaca gaaccctcc aaaaaagca aaacttgcg gttttgaaa aaggtctca aagactggc ttacccaaa ttcaacaca tcaaaaact aagaataat cgtcggcgg tcctctgca cataaacaa cgactgaga agccgacta tttaagagc tcggataag gaaaatcag agtaatgtt tcgaccaca taccacgat tttgagaaa gaaatatcg gcaagagat gcgtgcagg agtaccatc ggtggaacg attggtcta caggttaca aatccagag gggattcgc ataaagcag gaaaacttt gccattccc agtggcatt gctcccttg cccaggaca ccaaggccc aaaaatccg agcaaattt taa
- >TC005107 RA
taaacagtg acactgaag gacgttaaa acgaattta aaacttgtt accgtcctt tttatgtct ttaacgttt cactgtgtt tgtcagtaa aagtttcca agtttttaa gaaatatgt gcgacataa agcgccggc tgtctcaaa tggacgatt tcaacgaaa aaatgtttg agggcaagc cgtgggccg aaaaactgt tatctgaat cgataaaat tgacaaaaa aggcagtgg cgaactggt gccacatcg tgtgtaaaa tcggcgaag ccgaggtag acctggacg attatgtga caattttcc tccaacaaa tcgaaaccc tttacgaag acctgatcg ccgatgtgg acacaatcc acgagaaaa gtctcgata aaataatga aactgttgg agaaactcg aaaatctct gtcgcgacc tccacgtcc caatcccca ctttcaact acgaagggg ttcctctga gtgaggtgc gcctccaaa tcgtccaaa aggtggacg aattcgagg ctttggccc agattcgcc gacgggagt ttagcgagt tgcagcaga aagagctca aactgtgtg cctgtctgg ggcgggccc cccgactgc tccccgggg ccccactcc cgttgcccc ccgagctgg aggagttga aggcctatg tcgagaacc tggagacgg aggcgatgg aacgggagg aggttttct tccaactga aggaaaaaa tcgtcgaga ttgtctccg aactagaga tgtgcccta atctagagt tcgagaagg atattttga atttgtcca atacccaag tgcgcataa ccgacgcag aaatggagc agttgcggc agttccacg accagttgg tcaagcgcc tcgctgacg tgaccgagg aggttgaag agttgagga gcaggatcg acgatttat ggaaaaaat tggaggttg atttggtgg agcgtgatg atttccggg cgagacacg tggggcact cgttgaaga cgctggagg cgcttaaag aggagttga ataggttga aggcgatga aaaaggcca agcttcaga gtttcattt taaagcaac gagatgagc tcgaagaat ggtgggaaa agtgtcact gtacacaaa aacaacgcg atgatttta cgttttact actccgatt gttttaccg aagatttgt tcgatatac atgagcgtg aaatcgaga aatggaaga attatcacg aggagaact ctgaaatct ttgcacttc tcaaggagt ttaagacgt tgtggacgg aacagatcg agctagaat cgaattcta aaggtgcta atcggtaca agaaccgcg gagggcaat tgttgattg aggaaagga aacgtaata agctcaaaa aacgacttc cagtagttg aggataagc tccgaagtt tggctcaag cgtatttgg tccgaaatg ggcaagaat tcgagacgt ttgggcaaa caatcgacg actacattc aacaactgc acgaaaata aagaacggg attttaaac agaaactga gtgccaaga aacaactgc gtgagcaac agccgtcct gttcgagca gactgccca caactccac gccactgct ccaaacgca aagtcacag cgactccag cgacagaac cctccaaaa aagcaaaac ttgcggttt tgaaaaagg tctcaaaga ctggcttac ccaaattca acacatcaa aaactaaga ataatcgtc ggcggtcct ctgcacata aacaacgac tgagaagcc gactattta agagctcgg ataaggaaa atcagagta atgtttcga ccacatacc acgattttg agaaagaaa tatcggcaa gagatgcgt gcaggagta ccatcggtg gaacgattg gtctacagg ttacaaatc cagagggga ttcgcataa agcaggaaa actttgcca ttcccagtg gcattgctc ccttgccca ggacaccaa ggcccaaaa atccgagca aattttaat taatcgact gttacattt tgtttttgt attattttt acgcacatg tacttaaaa ggagtttag tttttaaat tacgtccat ttgtttttt atttaaagg tgctcctct attatatgt tacaaatta ctaaatata tttattgca tgact
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
