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Gene information
General information
TC005107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid464: 6176..10352
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005107
    iBeetle screen iB_03907
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    egg chamber potentially fused - Number of animals showing the phenotype: 3
    tropharium potentially shape irregular - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005107 RA
      MCDIKRRLS QMDDFNEKM FEGKPWAEK LLSESIKLT 
      KKAVANWCH IVCKIGEAE VDLDDYVTI FLQQIETLY 
      EDLIADVDT IHEKSLDKI MKLLEKLEN LCRDLHVPI 
      PTFNYEGVP LSEVRLQIV QKVDEFEAL AQIRRREFS 
      ELQQKELKL CACLGRAPR LLPGAPLPL PPELEELKA 
      YVENLETEA MEREEVFFQ LKEKIVEIV SELEMCPNL 
      EFEKDILNL SNTQVRITD AEMEQLRQF HDQLVKRLA 
      DVTEEVEEL RSRIDDLWK KLEVDLVER DDFRARHVG 
      HSLKTLEAL KEELNRLKA MKKAKLQSF ILKQRDELE 
      EWWEKCHCT QKQRDDFTF YYSDCFTED LFDIHEREI 
      EKWKNYHEE NSEIFALLK EFKTLWTEQ IELESNSKG 
      ANRYKNRGG QLLIEERKR NKLKKRLPV VEDKLRSLA 
      QAYLVRNGQ EFETFGQTI DDYIQQLHE NKERDFKQK 
      LSAKKQLRE QQPSCSSRL PTTPRHCSK RKVTATPAT 
      EPSKKAKLA VLKKVSKTG LPKFNTSKT KNNRRRSSA 
      HKQRLRSRL FKSSDKENQ SNVSTTYHD FEKEISARD 
      ACRSTIGGT IGLQVTNPE GIRIKQENF AIPSGIAPL 
      PRTPRPKNP SKF
    CDS sequence
    • >TC005107 RA
      atgtgcgac ataaagcgc cggctgtct caaatggac 
      gatttcaac gaaaaaatg tttgagggc aagccgtgg 
      gccgaaaaa ctgttatct gaatcgata aaattgaca 
      aaaaaggca gtggcgaac tggtgccac atcgtgtgt 
      aaaatcggc gaagccgag gtagacctg gacgattat 
      gtgacaatt ttcctccaa caaatcgaa accctttac 
      gaagacctg atcgccgat gtggacaca atccacgag 
      aaaagtctc gataaaata atgaaactg ttggagaaa 
      ctcgaaaat ctctgtcgc gacctccac gtcccaatc 
      cccactttc aactacgaa ggggttcct ctgagtgag 
      gtgcgcctc caaatcgtc caaaaggtg gacgaattc 
      gaggctttg gcccagatt cgccgacgg gagtttagc 
      gagttgcag cagaaagag ctcaaactg tgtgcctgt 
      ctggggcgg gccccccga ctgctcccc ggggcccca 
      ctcccgttg ccccccgag ctggaggag ttgaaggcc 
      tatgtcgag aacctggag acggaggcg atggaacgg 
      gaggaggtt ttcttccaa ctgaaggaa aaaatcgtc 
      gagattgtc tccgaacta gagatgtgc cctaatcta 
      gagttcgag aaggatatt ttgaatttg tccaatacc 
      caagtgcgc ataaccgac gcagaaatg gagcagttg 
      cggcagttc cacgaccag ttggtcaag cgcctcgct 
      gacgtgacc gaggaggtt gaagagttg aggagcagg 
      atcgacgat ttatggaaa aaattggag gttgatttg 
      gtggagcgt gatgatttc cgggcgaga cacgtgggg 
      cactcgttg aagacgctg gaggcgctt aaagaggag 
      ttgaatagg ttgaaggcg atgaaaaag gccaagctt 
      cagagtttc attttaaag caacgagat gagctcgaa 
      gaatggtgg gaaaagtgt cactgtaca caaaaacaa 
      cgcgatgat tttacgttt tactactcc gattgtttt 
      accgaagat ttgttcgat atacatgag cgtgaaatc 
      gagaaatgg aagaattat cacgaggag aactctgaa 
      atctttgca cttctcaag gagtttaag acgttgtgg 
      acggaacag atcgagcta gaatcgaat tctaaaggt 
      gctaatcgg tacaagaac cgcggaggg caattgttg 
      attgaggaa aggaaacgt aataagctc aaaaaacga 
      cttccagta gttgaggat aagctccga agtttggct 
      caagcgtat ttggtccga aatgggcaa gaattcgag 
      acgtttggg caaacaatc gacgactac attcaacaa 
      ctgcacgaa aataaagaa cgggatttt aaacagaaa 
      ctgagtgcc aagaaacaa ctgcgtgag caacagccg 
      tcctgttcg agcagactg cccacaact ccacgccac 
      tgctccaaa cgcaaagtc acagcgact ccagcgaca 
      gaaccctcc aaaaaagca aaacttgcg gttttgaaa 
      aaggtctca aagactggc ttacccaaa ttcaacaca 
      tcaaaaact aagaataat cgtcggcgg tcctctgca 
      cataaacaa cgactgaga agccgacta tttaagagc 
      tcggataag gaaaatcag agtaatgtt tcgaccaca 
      taccacgat tttgagaaa gaaatatcg gcaagagat 
      gcgtgcagg agtaccatc ggtggaacg attggtcta 
      caggttaca aatccagag gggattcgc ataaagcag 
      gaaaacttt gccattccc agtggcatt gctcccttg 
      cccaggaca ccaaggccc aaaaatccg agcaaattt 
      taa
    mRNA sequence
    • >TC005107 RA
      taaacagtg acactgaag gacgttaaa acgaattta 
      aaacttgtt accgtcctt tttatgtct ttaacgttt 
      cactgtgtt tgtcagtaa aagtttcca agtttttaa 
      gaaatatgt gcgacataa agcgccggc tgtctcaaa 
      tggacgatt tcaacgaaa aaatgtttg agggcaagc 
      cgtgggccg aaaaactgt tatctgaat cgataaaat 
      tgacaaaaa aggcagtgg cgaactggt gccacatcg 
      tgtgtaaaa tcggcgaag ccgaggtag acctggacg 
      attatgtga caattttcc tccaacaaa tcgaaaccc 
      tttacgaag acctgatcg ccgatgtgg acacaatcc 
      acgagaaaa gtctcgata aaataatga aactgttgg 
      agaaactcg aaaatctct gtcgcgacc tccacgtcc 
      caatcccca ctttcaact acgaagggg ttcctctga 
      gtgaggtgc gcctccaaa tcgtccaaa aggtggacg 
      aattcgagg ctttggccc agattcgcc gacgggagt 
      ttagcgagt tgcagcaga aagagctca aactgtgtg 
      cctgtctgg ggcgggccc cccgactgc tccccgggg 
      ccccactcc cgttgcccc ccgagctgg aggagttga 
      aggcctatg tcgagaacc tggagacgg aggcgatgg 
      aacgggagg aggttttct tccaactga aggaaaaaa 
      tcgtcgaga ttgtctccg aactagaga tgtgcccta 
      atctagagt tcgagaagg atattttga atttgtcca 
      atacccaag tgcgcataa ccgacgcag aaatggagc 
      agttgcggc agttccacg accagttgg tcaagcgcc 
      tcgctgacg tgaccgagg aggttgaag agttgagga 
      gcaggatcg acgatttat ggaaaaaat tggaggttg 
      atttggtgg agcgtgatg atttccggg cgagacacg 
      tggggcact cgttgaaga cgctggagg cgcttaaag 
      aggagttga ataggttga aggcgatga aaaaggcca 
      agcttcaga gtttcattt taaagcaac gagatgagc 
      tcgaagaat ggtgggaaa agtgtcact gtacacaaa 
      aacaacgcg atgatttta cgttttact actccgatt 
      gttttaccg aagatttgt tcgatatac atgagcgtg 
      aaatcgaga aatggaaga attatcacg aggagaact 
      ctgaaatct ttgcacttc tcaaggagt ttaagacgt 
      tgtggacgg aacagatcg agctagaat cgaattcta 
      aaggtgcta atcggtaca agaaccgcg gagggcaat 
      tgttgattg aggaaagga aacgtaata agctcaaaa 
      aacgacttc cagtagttg aggataagc tccgaagtt 
      tggctcaag cgtatttgg tccgaaatg ggcaagaat 
      tcgagacgt ttgggcaaa caatcgacg actacattc 
      aacaactgc acgaaaata aagaacggg attttaaac 
      agaaactga gtgccaaga aacaactgc gtgagcaac 
      agccgtcct gttcgagca gactgccca caactccac 
      gccactgct ccaaacgca aagtcacag cgactccag 
      cgacagaac cctccaaaa aagcaaaac ttgcggttt 
      tgaaaaagg tctcaaaga ctggcttac ccaaattca 
      acacatcaa aaactaaga ataatcgtc ggcggtcct 
      ctgcacata aacaacgac tgagaagcc gactattta 
      agagctcgg ataaggaaa atcagagta atgtttcga 
      ccacatacc acgattttg agaaagaaa tatcggcaa 
      gagatgcgt gcaggagta ccatcggtg gaacgattg 
      gtctacagg ttacaaatc cagagggga ttcgcataa 
      agcaggaaa actttgcca ttcccagtg gcattgctc 
      ccttgccca ggacaccaa ggcccaaaa atccgagca 
      aattttaat taatcgact gttacattt tgtttttgt 
      attattttt acgcacatg tacttaaaa ggagtttag 
      tttttaaat tacgtccat ttgtttttt atttaaagg 
      tgctcctct attatatgt tacaaatta ctaaatata 
      tttattgca tgact