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Gene information
General information
TC005104 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1835516..1854930
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005104
    iBeetle screen iB_03905

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: only six larvae on the slide showing this phenotype
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: only six larvae on the slide showing this phenotype
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: only six larvae on the slide showing this phenotype
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005104 RA
      MEREKQPLS TTIPSAVHD AAVQASWEP PHGGSLLPV 
      GETSGERAY VPTVGHCAL LGGPAVPPF VGGPFDIFT 
      TNNNAGDLC ELIERTQIE GSWPIDHPL PLPRWSCKS 
      QKCYQLETL THFENLAAK IELHVQRLL EEHNYNTVG 
      NFIDLYQNF KKSGCCNFF KYFQSYAPP ITPAHHTCV 
      GLALELWNR LHQLEVSFP EISQHLFLV SCEENIEAL 
      SEYTALSER LDTAAYDLE KEHVLLCLR FKINERQGL 
      LLCDPGYHV SRVVTIMQD RAYPNTGWF IQSEENNIR 
      KEYNYQFSP LNEKFVEWN ERTTRNGIQ EIFTGLIYV 
      AHPYLTAVD VTERRNLVY NFRSLLARD QKGHLIAGV 
      YFKVKENCD EFTIFYQDM GKQRMKMKF STLTQPFQV 
      KEKALNIIT ICNEQLNLP EGSLLDVLL QVGDLMRDI 
      SYLRQLLEV NESINIMSA NN
    CDS sequence
    • >TC005104 RA
      atggagcgt gagaaacaa ccgctaagt accacaata 
      ccatcagct gttcacgat gcagctgtc caagcaagc 
      tgggagccc ccacatggg ggctccttg ctccccgtt 
      ggtgagaca agcggcgag cgtgcctac gtacccacc 
      gtgggccat tgcgccctc ctagggggg ccagcggtg 
      ccccctttt gtagggggg ccttttgac attttcacc 
      accaataac aacgctggc gacttgtgc gaactcatc 
      gaaagaaca caaatcgaa ggatcatgg cccattgat 
      catccactg ccattgccc agatggtcg tgcaagtcc 
      caaaaatgc taccagctg gaaacgcta acccatttt 
      gagaatttg gccgccaaa atcgaacta cacgtgcag 
      aggttgttg gaggaacat aattacaac accgttggc 
      aatttcatt gatttgtac cagaatttt aagaagagt 
      ggatgttgc aatttcttc aaatatttt caaagttat 
      gcgcctcca atcactcca gcccaccac acatgcgtc 
      ggcttggct ttagaatta tggaatcgt cttcatcag 
      ctcgaagtg agctttccg gagattagt cagcacctg 
      tttttagta tcatgtgag gaaaatatt gaagcttta 
      agcgaatac acagccttg agtgaacga ttagacact 
      gccgcttac gacttagaa aaagagcac gtcctctta 
      tgcctccga tttaaaatt aacgaacgg caaggatta 
      ttattatgc gatcccggt tatcatgtt agtagagtt 
      gtcaccatc atgcaagat cgagcatat cctaacacc 
      ggttggttc atccaatcg gaagaaaac aacatccgg 
      aaagaatac aactatcaa ttctcgcct ttgaacgag 
      aaattcgtt gaatggaac gagcgaaca acaaggaat 
      ggaatacag gaaattttc actggattg atctacgtt 
      gcacatcct tatttgacc gcagttgat gtgacagaa 
      cgcagaaat ttggtctac aatttcagg agtttgttg 
      gcaagggat caaaaggga cacttgata gccggtgtt 
      tactttaaa gtgaaagag aactgcgat gaattcaca 
      atcttctat caagatatg ggcaaacag aggatgaaa 
      atgaaattc tcgacactc actcaaccg tttcaggtg 
      aaagagaaa gcacttaac attatcacc atttgcaac 
      gagcagtta aatctaccg gaaggttca cttttggat 
      gttttgctt caagtggga gatttgatg agggatata 
      tcgtatttg cgacagctt ttggaggtt aatgagagt 
      attaacatc atgtcggct aacaactag
    mRNA sequence
    • >TC005104 RA
      gtcgagacg ttactggag agacactca gttgatgga 
      aatgtcgcc taaaataca cgcccattt ctaaaagat 
      atgatcaaa ttcggcaca ataatgcga tttccaagt 
      cccgaagtc atcgtagtg attaataac aacttgtgc 
      aattcccat aaacacttt gtttatcag taccttttg 
      cggactgtg tgctacagt gattaagtg ctgttaaaa 
      cgtcacttg atagacagc tccattgtt tagcgactt 
      tacgtcaca cgagtgggg ggaccccca accttttaa 
      atatccccc atctacctt caaagataa ttcagttta 
      ttacaccga agtagagcg agcagagta gtgcgctac 
      aacctcgtt gtttcttcg tttcttatt tttattttg 
      accgttgta tggagcgtg agaaacaac cgctaagta 
      ccacaatac catcagctg ttcacgatg cagctgtcc 
      aagcaagct gggagcccc cacatgggg gctccttgc 
      tccccgttg gtgagacaa gcggcgagc gtgcctacg 
      tacccaccg tgggccatt gcgccctcc taggggggc 
      cagcggtgc ccccttttg taggggggc cttttgaca 
      ttttcacca ccaataaca acgctggcg acttgtgcg 
      aactcatcg aaagaacac aaatcgaag gatcatggc 
      ccattgatc atccactgc cattgccca gatggtcgt 
      gcaagtccc aaaaatgct accagctgg aaacgctaa 
      cccattttg agaatttgg ccgccaaaa tcgaactac 
      acgtgcaga ggttgttgg aggaacata attacaaca 
      ccgttggca atttcattg atttgtacc agaatttta 
      agaagagtg gatgttgca atttcttca aatattttc 
      aaagttatg cgcctccaa tcactccag cccaccaca 
      catgcgtcg gcttggctt tagaattat ggaatcgtc 
      ttcatcagc tcgaagtga gctttccgg agattagtc 
      agcacctgt ttttagtat catgtgagg aaaatattg 
      aagctttaa gcgaataca cagccttga gtgaacgat 
      tagacactg ccgcttacg acttagaaa aagagcacg 
      tcctcttat gcctccgat ttaaaatta acgaacggc 
      aaggattat tattatgcg atcccggtt atcatgtta 
      gtagagttg tcaccatca tgcaagatc gagcatatc 
      ctaacaccg gttggttca tccaatcgg aagaaaaca 
      acatccgga aagaataca actatcaat tctcgcctt 
      tgaacgaga aattcgttg aatggaacg agcgaacaa 
      caaggaatg gaatacagg aaattttca ctggattga 
      tctacgttg cacatcctt atttgaccg cagttgatg 
      tgacagaac gcagaaatt tggtctaca atttcagga 
      gtttgttgg caagggatc aaaagggac acttgatag 
      ccggtgttt actttaaag tgaaagaga actgcgatg 
      aattcacaa tcttctatc aagatatgg gcaaacaga 
      ggatgaaaa tgaaattct cgacactca ctcaaccgt 
      ttcaggtga aagagaaag cacttaaca ttatcacca 
      tttgcaacg agcagttaa atctaccgg aaggttcac 
      ttttggatg ttttgcttc aagtgggag atttgatga 
      gggatatat cgtatttgc gacagcttt tggaggtta 
      atgagagta ttaacatca tgtcggcta acaactagc 
      atatagcaa atacagatt ttcggtttg gttttgtgt 
      tctttttta ttgcggttg tcggtttgg ttttttact 
      tattttcac ttgtttgat taggttaga atttagttt 
      atatacagg gtgagtcta agttccttc atactctgt 
      aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY