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Gene information
General information
TC005103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1765097..1770283
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005103
    iBeetle screen iB_03904

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005103 RA
      MAYSWDNRV SFVVKYLYD TDNNGYLDE 
      RDFACLALR ATIIEGKGE FSFSRLQEY 
      QHIMLSLWE EIAELADFN KDGKITTEE 
      FKQAVQQCC MGRRYEDFP QAMKMFIDS 
      NFKMVDLND DGVIGADEY RFNCVTKFA 
      IDDIEVVDE AFNNLLSDD DRRRGGLTL 
      SRYQELYAQ FLGNPDDKC PAIYLFGPL 
      AEFQL
    CDS sequence
    • >TC005103 RA
      atggcttac agctgggat aacagagtt agtttcgtc 
      gtcaaatac ctatacgat acggacaat aacggttat 
      ttggatgag cgcgatttc gcctgtctg gccctaagg 
      gcgacgata attgagggt aaaggagag ttcagcttc 
      tcacgtctt caggagtac caacatatc atgttgagt 
      ttgtgggaa gagattgct gaattagcc gatttcaat 
      aaggacggc aaaatcacc accgaagaa ttcaagcag 
      gcggtgcaa caatgttgc atgggccga agatatgag 
      gacttcccc caagccatg aaaatgttc atcgactca 
      aatttcaaa atggttgat ttgaacgat gatggagtt 
      atcggagcg gacgaatat agattcaac tgtgtgacg 
      aaattcgca atagacgac atcgaagtg gtcgatgaa 
      gctttcaac aacttatta agcgatgat gataggcga 
      agaggcggc ttgacttta tcacgttac caggaattg 
      tacgctcag tttcttggc aaccctgat gacaaatgc 
      cccgccatc tatctcttc ggaccgtta gcagaattc 
      caactttaa
    mRNA sequence
    • >TC005103 RA
      acctgacgc aaactcgac ttttcaaaa atagacacc 
      ggccggtcc gttcatttt tgccacagt acaaacaca 
      tttcggttc tagtgtagt tctctgtgt ttgatttta 
      ttaagtttc aatggctta cagctggga taacagagt 
      tagtttcgt cgtcaaata cctatacga tacggacaa 
      taacggtta tttggatga gcgcgattt cgcctgtct 
      ggccctaag ggcgacgat aattgaggg taaaggaga 
      gttcagctt ctcacgtct tcaggagta ccaacatat 
      catgttgag tttgtggga agagattgc tgaattagc 
      cgatttcaa taaggacgg caaaatcac caccgaaga 
      attcaagca ggcggtgca acaatgttg catgggccg 
      aagatatga ggacttccc ccaagccat gaaaatgtt 
      catcgactc aaatttcaa aatggttga tttgaacga 
      tgatggagt tatcggagc ggacgaata tagattcaa 
      ctgtgtgac gaaattcgc aatagacga catcgaagt 
      ggtcgatga agctttcaa caacttatt aagcgatga 
      tgataggcg aagaggcgg cttgacttt atcacgtta 
      ccaggaatt gtacgctca gtttcttgg caaccctga 
      tgacaaatg ccccgccat ctatctctt cggaccgtt 
      agcagaatt ccaacttta aggctgatg ttagataat 
      ttaagtgtt gttgttttt gttgcattt tttaaacaa 
      taaattttt gggtaataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY