Status
Please wait ...
Gene information
General information
TC033497
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005101
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 1745707..1747649
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033497
    iBeetle screen iB_03903

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033497 RA
      MYQDINHGI IEIGCPTRD KVVEITKPY FKALKSFSL 
      ESNSVHEVC RICFRLLKE EEIVKLYDE GPPSQETKE 
      LRDMLEFIL PELDLELYT NVVVCTKCR DLLAFSYEL 
      QHAWMCTEK KLQKVVEEK KSLTNLSSD LVIVTESSV 
      GDVMREHHQ DVINVDHDY EKNVENAPV LKRDEVQYV 
      ARRILDNIP EDPINVNED FLTKKKLKF FYRGVNLSS 
      YNLIGFRTK RKEVGTKWG EGPYICEMC AFFTKSLIS 
      FQRHLRKHA TNKLSGDIP KKKKPRIKS FKCDKCDQL 
      FTSQQHLER HFLKHQTYK CGICEKEFK LNSILRKHV 
      KHVHLKDYN YYQCHLCGK QFCYKDSLR LHFVTHNSV 
      KTVKCDLCP KKFATRVKL TKHLRSVHS DAPPLTCSY 
      CGKSIKNEK NLEKHIQVY HFHQGDICD ICGKPFSKK 
      DYLIKHKMR VHGVGTL
    CDS sequence
    • >TC033497 RA
      atgtatcaa gatattaat catggaata atcgaaatt 
      ggctgtccc acacgcgat aaagttgtt gaaataaca 
      aaaccctac ttcaaagca ctgaaaagt ttttcttta 
      gaaagtaat tcagtccac gaggtatgt cggatttgt 
      ttccgctta ttaaaagaa gaagaaatt gtcaaactg 
      tacgatgag gggcctcct tcccaagag acaaaagaa 
      ttgcgcgat atgttagaa tttattttg cccgaattg 
      gacctcgaa ttatataca aatgttgtt gtttgtacg 
      aaatgtaga gaccttttg gccttcagt tacgagtta 
      caacatgcc tggatgtgt acggagaag aaattgcaa 
      aaagtagtt gaagaaaag aaaagctta acaaattta 
      agctccgat ttagttatt gtaacggaa tcatctgtt 
      ggcgacgta atgagagaa caccatcaa gatgtaatt 
      aatgttgat catgattac gaaaagaat gttgaaaat 
      gcaccagtt ttgaaaaga gatgaagtc caatatgtg 
      gcgcgcaga attcttgat aatattccg gaagaccct 
      ataaatgta aatgaggat tttctaaca aagaagaag 
      ctgaaattc ttttacaga ggagttaat ttgagttcg 
      tataatctg attgggttc agaaccaaa agaaaagaa 
      gtcgggacc aaatggggt gagggacct tatatttgc 
      gaaatgtgt gcgtttttc accaaaagt ttgatttcc 
      ttccaacga catttgcgc aaacatgcg accaataaa 
      ctgtctggt gatattcca aagaagaaa aaaccaagg 
      attaaatcg ttcaaatgc gataaatgt gatcagttg 
      tttactagt caacagcat ctcgaacgg catttctta 
      aagcatcag acttataaa tgcggaata tgtgagaaa 
      gagtttaaa ctgaatagt atactacga aagcatgtt 
      aaacatgtg catttgaaa gattacaac tattaccag 
      tgccatttg tgcgggaaa caattttgt tacaaggat 
      tctttgagg ttgcacttc gtgacacat aattctgtt 
      aaaacagta aagtgtgat ttatgtccg aaaaagttt 
      gcaacgagg gttaaatta actaaacat cttcgatcc 
      gtacatagc gatgctcct ccattaact tgcagttat 
      tgtggcaag tctataaag aatgaaaag aatttggag 
      aaacatatt caggtgtat cattttcat caaggggat 
      atttgtgat atttgcggg aaaccattt tcgaaaaaa 
      gattatttg ataaaacat aaaatgcgg gttcatggt 
      gttggaaca ttgtga
    mRNA sequence
    • >TC033497 RA
      ttgtataaa tttacttta tgctcttaa ttattgtcc 
      taattgtta ttctaattc tgttatttc ttttcagag 
      aatcaaaga atccaaata gacctacca ataatacga 
      gccgataag gtaaatcgt caaaaattc aactttgaa 
      aatttagtt caaaatgtt aatccaata aaaacctca 
      ttaaaagaa gccttgggc gttttaacc gatccaaac 
      gtagaacct aatattttt cacgaagaa atacaacaa 
      tatgagact tttgataat gagagtgaa gaatgtgct 
      ccatcatgt ccctcacca cttccacct atggttccg 
      acaaacgtg cccccacct cttattatt ccgtcatat 
      agttcgatg tatcaagat attaatcat ggaataatc 
      gaaattggc tgtcccaca cgcgataaa gttgttgaa 
      ataacaaaa ccctacttc aaagcactg aaaagtttt 
      tctttagaa agtaattca gtccacgag gtatgtcgg 
      atttgtttc cgcttatta aaagaagaa gaaattgtc 
      aaactgtac gatgagggg cctccttcc caagagaca 
      aaagaattg cgcgatatg ttagaattt attttgccc 
      gaattggac ctcgaatta tatacaaat gttgttgtt 
      tgtacgaaa tgtagagac cttttggcc ttcagttac 
      gagttacaa catgcctgg atgtgtacg gagaagaaa 
      ttgcaaaaa gtagttgaa gaaaagaaa agcttaaca 
      aatttaagc tccgattta gttattgta acggaatca 
      tctgttggc gacgtaatg agagaacac catcaagat 
      gtaattaat gttgatcat gattacgaa aagaatgtt 
      gaaaatgca ccagttttg aaaagagat gaagtccaa 
      tatgtggcg cgcagaatt cttgataat attccggaa 
      gaccctata aatgtaaat gaggatttt ctaacaaag 
      aagaagctg aaattcttt tacagagga gttaatttg 
      agttcgtat aatctgatt gggttcaga accaaaaga 
      aaagaagtc gggaccaaa tggggtgag ggaccttat 
      atttgcgaa atgtgtgcg tttttcacc aaaagtttg 
      atttccttc caacgacat ttgcgcaaa catgcgacc 
      aataaactg tctggtgat attccaaag aagaaaaaa 
      ccaaggatt aaatcgttc aaatgcgat aaatgtgat 
      cagttgttt actagtcaa cagcatctc gaacggcat 
      ttcttaaag catcagact tataaatgc ggaatatgt 
      gagaaagag tttaaactg aatagtata ctacgaaag 
      catgttaaa catgtgcat ttgaaagat tacaactat 
      taccagtgc catttgtgc gggaaacaa ttttgttac 
      aaggattct ttgaggttg cacttcgtg acacataat 
      tctgttaaa acagtaaag tgtgattta tgtccgaaa 
      aagtttgca acgagggtt aaattaact aaacatctt 
      cgatccgta catagcgat gctcctcca ttaacttgc 
      agttattgt ggcaagtct ataaagaat gaaaagaat 
      ttggagaaa catattcag gtgtatcat tttcatcaa 
      ggggatatt tgtgatatt tgcgggaaa ccattttcg 
      aaaaaagat tatttgata aaacataaa atgcgggtt 
      catggtgtt ggaacattg tgaaactac ttttaaata 
      ataattttt tataatggc atttgattt tgttatttc 
      aaacccttg acctaccca aatccctac tacatttga 
      taagtgatc ttaaataca caaattttg tattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY