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Gene information
General information
TC005089 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 1419896..1425473
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005089
    iBeetle screen iB_03901
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005089 RA
      MHKDSDTQK NISPHFKKR SGTTDRGKD YEHLFIAEA 
      SLKLLKDAQ VEDFYISSN DEKFGSFDD VVLEIKHHN 
      IPDIHTYAL QLKHKKNNV LKIEDLTAK KGNFSISEY 
      YKHTKLEGP VKTILFTNC KLQKNSNEF QVILNETVT 
      VDVVIETSK PEDLVNTAP NGQCYKFKS DPKYENFFE 
      NFFLYVGQS SSEHLRTKC LDTFQRMYQ CDESVFNEY 
      LTFLTNWSM IEDKKEKLT KSWIKRVIV HQVLSPFIK 
      PLSFQFSNV EKKQKMLQN AMSNFKVTI LGEGGFDKV 
      RRLWRDAAL EVDMKELNK ICRKYSVGS KYIEKIDDS 
      VEISKLLWL MDKCPLVVE GDPKTLPAI DLSDLGRFV 
      IVNPQMQLA ERPHFQQLS HLKNEEPVI FEDIVNNFT 
      FSLEGQRET LLKVLLHQD EEFENIITT DHLVQMLVG 
      PLVIGEVKE VLPSYYVAR TMSRIMIKL QYLSEFHEN 
      TVVIISQVQ NLEMFKSRF KQLKIVVIG TKEECGDGN 
      KVIYVCQEE CTQEAFEEL CSQGVNCHH FRYFSEGLE 
      WIRSKNGVE ELRNFQIND YKDLTESEL FDSSSNLVN 
      VICENPGMG KTVLMKSLK INRKCWTIM IYSRNHGSY 
      FRKNGPDVE HFLNYIVDA NCKHYTTFD RTVYKKLAR 
      SRNFELIWD GLDEVSDTT LRHITSVIH GITKKGHKQ 
      WITSRNNLV QRLEREFGV FSRSVHQFK EEEQKSYIR 
      DRLSCSEDE LSSIFEKIQ SNIQLFPKN TILGIPLQI 
      YMITELFFE NIDKYLALL SQIFTVADL YEHFVHKII 
      YDNYKEKKD FNFDIDANW DEFEEKKIT MLEHYQRIA 
      LQVYFEDDP QIPLSKDAI GFIVNVGDG NGQFLHNSY 
      GEYFAALFF TKRENFAKI PPEFYVDEW FNNIRFFRD 
      LILAQSCKA HIAVLYKNP QLLEQCAQN DILQKDAAG 
      RSSFQVSCT WSKKYPRLE TMKRSNYYF ITNTAIYNL 
      SKGDFEHFD PNDNSLPSK FDKICDLTL RKILLLFPF 
      MTPFWESIF DEFHVPSLL YYAVKFDYD LVFKSFDQI 
      PFIKISNSS TTLLDFAIE SLAFNCITH LMSNQLYKS 
      RVFEDEKCS FKICILQST KLLRIFENH GWDINQLHR 
      GIGILHYIC NHGGDLTPF NFLLQNGIQ INKPDENGK 
      LPIHYACLK NDMYLVELL LMNGARGDI PDENGKTVI 
      NYACKSEEY GPSILKLLF QSGLKSDQS VLIDAVSHH 
      GIEIVKILV DNADISDGN QLLHHACKN ERNGYEIIK 
      FLLGKGVKV DKPDSDGKL LIHLIAERG WADLIQNDN 
      VNVCDNCGK MPIHYACSG GHFTIVEVL IQNGATVNV 
      CDNCGKMPI HYACSGGHF TIVEALIQN GAKVNVCDD 
      RGKTPIHYA CQSESTEIV ALLIQNGAT VNAEDGEGI 
      LPIYSSTRS DIIKLLIQN GANVNMPYP DGTFLIHFA 
      ALRGWTSDI AMFLIQQGT DLNIYDKYN QTALHYACR 
      YGRLEITNL LLQNGAKLT YDADDQTPI HYACRLDSF 
      DVLELFIQS GANINIPGK FGKMPIHIA SELGNIDIL 
      KNLIQHGCE IEIQDNKGL YPIHYACRN PFNGDQVVK 
      ILILNEANV NVVDHDGKF PIHYACQNE DYGYDIVRI 
      IFSRQDIIS LEDYKTLFD YVCKNRLNG FEIGKFLIK 
      MSWLGVVMA LVQKGVNRD IIDSKGRRR IDCVPKDSL 
      HYKEFLRVL QNTDQI
    CDS sequence
    • >TC005089 RA
      atgcacaag gacagtgat acgcaaaaa aatatttcc 
      ccgcatttc aaaaagagg tctggaacc acagacagg 
      ggcaaagac tacgaacac ttatttata gcagaagca 
      agtttaaaa ttactcaaa gatgcacaa gttgaggat 
      ttctacata tcttcaaat gacgaaaaa tttggttca 
      tttgacgac gtggtactc gaaataaaa caccacaac 
      atacctgac atacataca tatgccctc caactgaag 
      cacaaaaag aacaacgtt ttaaaaatt gaagatctt 
      actgcaaaa aagggtaat tttagtatt agcgaatac 
      tacaagcat accaaactg gaaggtcca gttaaaacg 
      attttattt actaactgc aaacttcaa aaaaactca 
      aacgaattt caagttatt ttgaacgaa acggtaacg 
      gttgatgtg gtaattgaa acttctaaa ccggaagat 
      ttagtaaac actgcgccc aatggccag tgttacaaa 
      ttcaaatca gatccaaag tatgagaat ttttttgaa 
      aactttttc ttgtacgtt ggacaatca agctcggaa 
      cacttacga acaaaatgt ctcgacaca tttcaacgc 
      atgtatcaa tgtgatgaa tcggtgttt aacgaatat 
      ctcacgttt ttaaccaac tggagtatg attgaagac 
      aagaaggaa aaattaacc aaatcgtgg attaaacgt 
      gtgatcgtc caccaagtg ttgtcgcct ttcattaaa 
      cctttgtca ttccaattt tcaaacgta gaaaagaaa 
      caaaaaatg ctccaaaat gccatgtca aattttaaa 
      gtaacaata cttggtgaa ggaggtttt gacaaagtg 
      aggagactc tggcgagat gctgcgctt gaagtggac 
      atgaaggaa cttaacaaa atctgcaga aaatattca 
      gtggggtcc aaatacata gaaaaaatt gatgactca 
      gttgagatc agcaaatta ttgtggctc atggataag 
      tgtcctttg gtggtcgaa ggggatcca aaaacattg 
      ccagcaatt gatttaagt gaccttgga cggtttgtc 
      atcgtaaat ccacagatg caattggcg gaaaggcct 
      cattttcaa caactatct catttaaaa aatgaagaa 
      ccggtgata tttgaagac attgtaaac aacttcacc 
      ttttccctg gaaggccaa agggaaaca ctgttgaaa 
      gtcttactc caccaagac gaagagttt gaaaatatt 
      atcacaacc gatcacttg gtgcaaatg cttgttggt 
      cctcttgta attggtgag gtcaaggaa gttttgcct 
      tcttactac gtcgcaagg acaatgtcc cgaatcatg 
      atcaagttg caatatttg agcgaattc cacgaaaac 
      acagtggtt ataatttcg caggtgcaa aatctcgaa 
      atgtttaaa tcacgtttc aagcaactt aaaattgtg 
      gtgattggg accaaggaa gagtgtgga gatggtaat 
      aaagtcatt tatgtgtgt caagaggag tgtactcaa 
      gaagcgttc gaagaattg tgctctcaa ggggtaaac 
      tgtcaccac tttagatat ttcagcgaa ggtttggag 
      tggattcgg agcaagaac ggtgtcgag gagctgcgc 
      aattttcaa attaacgat tataaagac ttgacagag 
      tcggaatta tttgactca tcaagtaac ctagttaac 
      gtcatttgt gagaaccct ggaatgggc aaaacagtt 
      ctgatgaaa agtctgaaa attaacagg aaatgttgg 
      acaatcatg atctattca cgaaatcat ggttcctac 
      ttccggaaa aatgggcca gacgttgaa cactttcta 
      aactatatt gtggacgcc aactgcaaa cactacaca 
      acttttgat agaacagtt tacaaaaag ttggcaagg 
      agcagaaat ttcgagttg atttgggac ggtctggat 
      gaagtttct gacaccacc ttgaggcat atcacaagt 
      gttattcat ggtatcacg aaaaaaggg cacaaacag 
      tggattacg tctcgcaac aatttggtc caaagattg 
      gaaagggag tttggcgtt ttttcccgt tcagttcat 
      cagttcaag gaggaggaa caaaagagt tatattcga 
      gatcggtta agttgttcc gaagatgag ttgtcttca 
      atttttgaa aaaatccaa tcaaacata caactcttc 
      cccaaaaac accattcta gggataccg ctacaaatc 
      tatatgatc accgaacta tttttcgaa aatatcgac 
      aagtactta gccctcttg tcgcaaatc ttcacggtc 
      gcagacttg tacgaacac tttgtgcac aaaatcatt 
      tacgataac tacaaggag aagaaggat ttcaatttc 
      gacattgac gccaattgg gacgaattc gaagagaag 
      aaaataaca atgttggaa cattaccaa agaattgcg 
      cttcaagtg tatttcgag gatgatcca caaatccct 
      ttaagcaaa gacgctatt ggtttcatt gtaaacgtt 
      ggcgatggc aatggtcaa tttttgcac aactcgtac 
      ggcgagtac tttgccgcg ttgtttttc acaaagcgc 
      gaaaatttt gcaaaaata ccaccagaa ttctatgtt 
      gacgaatgg tttaacaac attcgattt tttcgcgat 
      ttgatattg gctcaaagt tgcaaagcc cacattgct 
      gtcttgtac aaaaacccc cagttgttg gaacaatgc 
      gcccaaaac gatatttta cagaaagat gcagctggg 
      cggagcagt ttccaagtt tcgtgcact tggagtaaa 
      aagtaccca cgtttggag acaatgaaa cgctccaac 
      tattatttt attactaat accgccatt tataactta 
      agcaaaggc gatttcgaa cattttgac ccaaacgac 
      aactcctta ccgtcaaaa ttcgacaaa atatgcgac 
      ctgacattg cgaaaaatt ctgttactg ttcccgttt 
      atgacaccg ttctgggaa agtattttt gacgagttc 
      cacgtccct tcacttctg tactatgca gtcaaattt 
      gattacgac ttggttttt aagtcgttt gaccaaatt 
      ccttttatc aaaatatcg aattcctca acaacactg 
      ttagatttt gctatagaa tcactagct ttcaattgc 
      ataacccat ctgatgtcc aaccaattg tacaaaagt 
      cgtgtcttt gaagacgaa aaatgttca ttcaaaatt 
      tgcatccta caatcaact aaattgttg agaattttc 
      gaaaatcat ggatgggac ataaaccag ttgcaccgc 
      ggaattggg attttgcat tatatttgt aaccatggt 
      ggtgactta acaccattc aatttcttg ctccaaaat 
      ggaatccaa attaataaa cctgacgaa aacggcaag 
      ttacccatt cattatgct tgtttgaaa aacgacatg 
      tacttagta gaattgttg cttatgaat ggtgccaga 
      ggtgatatt ccagacgaa aacggtaaa actgtcatc 
      aattatgcc tgcaaaagt gaagagtac ggtccctca 
      attttaaag ttattgttc caaagtggt ttaaaaagt 
      gaccaaagt gttctaatt gacgccgtt tcacatcac 
      ggcattgaa attgtcaaa atccttgtc gataatgcc 
      gatatttct gacggaaat cagttgtta catcatgcc 
      tgtaagaac gaacgaaac ggttacgaa atcattaaa 
      ttcctgctt ggtaaaggt gtaaaagtt gataaacct 
      gattcagac ggcaaattg ttgattcac ttaatcgca 
      gagcgcggt tgggccgat ttgattcaa aatgacaat 
      gtgaatgtg tgtgacaac tgtggtaaa atgcccatt 
      cactatgcc tgcagtggt gggcatttt accattgtc 
      gaagtgttg attcaaaac ggcgcaacg gtgaatgtg 
      tgtgacaac tgtggtaaa atgcccatt cactatgcc 
      tgcagtggt gggcatttt actattgtc gaagcgttg 
      attcaaaac ggtgcaaag gtaaatgtg tgtgatgat 
      cggggtaaa acgcccatt cattatgcc tgccaaagt 
      gaatcaacc gaaatcgtg gcgttactt attcaaaat 
      ggcgctact gtgaatgct gaagatggg gaaggcata 
      ttaccaatt tattcctca acacggagc gacattatc 
      aagttgctt atccaaaat ggggccaac gtgaatatg 
      ccttatcca gacggaaca tttttaatt cactttgct 
      gctttgagg ggctggaca tcagatatt gcaatgttt 
      ctaattcag caaggaaca gatttaaat atttatgac 
      aaatacaac caaacggcg ttacattat gcttgtcgt 
      tacggtcgt ctggaaatc acaaatttg ttgcttcaa 
      aatggcgcc aaattaact tatgatgca gatgatcaa 
      actccaatt cactacgct tgtaggtta gattctttt 
      gatgttctc gagttgttt atccaaagt ggtgcaaac 
      ataaatatt cctggcaag tttggtaaa atgcccatc 
      catattgcc tccgaattg ggaaatatc gatatattg 
      aagaatttg atacaacac ggttgtgag atcgaaatc 
      caggacaat aaggggttg tatccaatt cattacgct 
      tgtcgcaat ccatttaac ggggaccaa gttgtaaaa 
      attttgata ttaaacgaa gcaaatgtg aatgtggtt 
      gatcatgat ggcaaattt ccaatacat tatgcgtgt 
      cagaatgag gattatggg tacgatatt gttcgaata 
      attttttcg cgacaagat atcatttcg ttagaggac 
      tataaaaca cttttcgat tatgtctgc aaaaatcgt 
      ttgaacggt tttgagatt gggaaattt ttgataaag 
      atgtcttgg ctgggtgtt gttatggca cttgtccag 
      aaaggtgtc aatagagat attattgat agtaagggg 
      agaaggcgt attgactgt gtgcccaaa gattccctg 
      cattataag gagtttctg agagtgttg caaaacact 
      gatcagata tag
    mRNA sequence
    • >TC005089 RA
      tgaaaaaca acacacgag gatgttatt gaataattc 
      gttttaata aaaaacata atgcacaag gacagtgat 
      acgcaaaaa aatatttcc ccgcatttc aaaaagagg 
      tctggaacc acagacagg ggcaaagac tacgaacac 
      ttatttata gcagaagca agtttaaaa ttactcaaa 
      gatgcacaa gttgaggat ttctacata tcttcaaat 
      gacgaaaaa tttggttca tttgacgac gtggtactc 
      gaaataaaa caccacaac atacctgac atacataca 
      tatgccctc caactgaag cacaaaaag aacaacgtt 
      ttaaaaatt gaagatctt actgcaaaa aagggtaat 
      tttagtatt agcgaatac tacaagcat accaaactg 
      gaaggtcca gttaaaacg attttattt actaactgc 
      aaacttcaa aaaaactca aacgaattt caagttatt 
      ttgaacgaa acggtaacg gttgatgtg gtaattgaa 
      acttctaaa ccggaagat ttagtaaac actgcgccc 
      aatggccag tgttacaaa ttcaaatca gatccaaag 
      tatgagaat ttttttgaa aactttttc ttgtacgtt 
      ggacaatca agctcggaa cacttacga acaaaatgt 
      ctcgacaca tttcaacgc atgtatcaa tgtgatgaa 
      tcggtgttt aacgaatat ctcacgttt ttaaccaac 
      tggagtatg attgaagac aagaaggaa aaattaacc 
      aaatcgtgg attaaacgt gtgatcgtc caccaagtg 
      ttgtcgcct ttcattaaa cctttgtca ttccaattt 
      tcaaacgta gaaaagaaa caaaaaatg ctccaaaat 
      gccatgtca aattttaaa gtaacaata cttggtgaa 
      ggaggtttt gacaaagtg aggagactc tggcgagat 
      gctgcgctt gaagtggac atgaaggaa cttaacaaa 
      atctgcaga aaatattca gtggggtcc aaatacata 
      gaaaaaatt gatgactca gttgagatc agcaaatta 
      ttgtggctc atggataag tgtcctttg gtggtcgaa 
      ggggatcca aaaacattg ccagcaatt gatttaagt 
      gaccttgga cggtttgtc atcgtaaat ccacagatg 
      caattggcg gaaaggcct cattttcaa caactatct 
      catttaaaa aatgaagaa ccggtgata tttgaagac 
      attgtaaac aacttcacc ttttccctg gaaggccaa 
      agggaaaca ctgttgaaa gtcttactc caccaagac 
      gaagagttt gaaaatatt atcacaacc gatcacttg 
      gtgcaaatg cttgttggt cctcttgta attggtgag 
      gtcaaggaa gttttgcct tcttactac gtcgcaagg 
      acaatgtcc cgaatcatg atcaagttg caatatttg 
      agcgaattc cacgaaaac acagtggtt ataatttcg 
      caggtgcaa aatctcgaa atgtttaaa tcacgtttc 
      aagcaactt aaaattgtg gtgattggg accaaggaa 
      gagtgtgga gatggtaat aaagtcatt tatgtgtgt 
      caagaggag tgtactcaa gaagcgttc gaagaattg 
      tgctctcaa ggggtaaac tgtcaccac tttagatat 
      ttcagcgaa ggtttggag tggattcgg agcaagaac 
      ggtgtcgag gagctgcgc aattttcaa attaacgat 
      tataaagac ttgacagag tcggaatta tttgactca 
      tcaagtaac ctagttaac gtcatttgt gagaaccct 
      ggaatgggc aaaacagtt ctgatgaaa agtctgaaa 
      attaacagg aaatgttgg acaatcatg atctattca 
      cgaaatcat ggttcctac ttccggaaa aatgggcca 
      gacgttgaa cactttcta aactatatt gtggacgcc 
      aactgcaaa cactacaca acttttgat agaacagtt 
      tacaaaaag ttggcaagg agcagaaat ttcgagttg 
      atttgggac ggtctggat gaagtttct gacaccacc 
      ttgaggcat atcacaagt gttattcat ggtatcacg 
      aaaaaaggg cacaaacag tggattacg tctcgcaac 
      aatttggtc caaagattg gaaagggag tttggcgtt 
      ttttcccgt tcagttcat cagttcaag gaggaggaa 
      caaaagagt tatattcga gatcggtta agttgttcc 
      gaagatgag ttgtcttca atttttgaa aaaatccaa 
      tcaaacata caactcttc cccaaaaac accattcta 
      gggataccg ctacaaatc tatatgatc accgaacta 
      tttttcgaa aatatcgac aagtactta gccctcttg 
      tcgcaaatc ttcacggtc gcagacttg tacgaacac 
      tttgtgcac aaaatcatt tacgataac tacaaggag 
      aagaaggat ttcaatttc gacattgac gccaattgg 
      gacgaattc gaagagaag aaaataaca atgttggaa 
      cattaccaa agaattgcg cttcaagtg tatttcgag 
      gatgatcca caaatccct ttaagcaaa gacgctatt 
      ggtttcatt gtaaacgtt ggcgatggc aatggtcaa 
      tttttgcac aactcgtac ggcgagtac tttgccgcg 
      ttgtttttc acaaagcgc gaaaatttt gcaaaaata 
      ccaccagaa ttctatgtt gacgaatgg tttaacaac 
      attcgattt tttcgcgat ttgatattg gctcaaagt 
      tgcaaagcc cacattgct gtcttgtac aaaaacccc 
      cagttgttg gaacaatgc gcccaaaac gatatttta 
      cagaaagat gcagctggg cggagcagt ttccaagtt 
      tcgtgcact tggagtaaa aagtaccca cgtttggag 
      acaatgaaa cgctccaac tattatttt attactaat 
      accgccatt tataactta agcaaaggc gatttcgaa 
      cattttgac ccaaacgac aactcctta ccgtcaaaa 
      ttcgacaaa atatgcgac ctgacattg cgaaaaatt 
      ctgttactg ttcccgttt atgacaccg ttctgggaa 
      agtattttt gacgagttc cacgtccct tcacttctg 
      tactatgca gtcaaattt gattacgac ttggttttt 
      aagtcgttt gaccaaatt ccttttatc aaaatatcg 
      aattcctca acaacactg ttagatttt gctatagaa 
      tcactagct ttcaattgc ataacccat ctgatgtcc 
      aaccaattg tacaaaagt cgtgtcttt gaagacgaa 
      aaatgttca ttcaaaatt tgcatccta caatcaact 
      aaattgttg agaattttc gaaaatcat ggatgggac 
      ataaaccag ttgcaccgc ggaattggg attttgcat 
      tatatttgt aaccatggt ggtgactta acaccattc 
      aatttcttg ctccaaaat ggaatccaa attaataaa 
      cctgacgaa aacggcaag ttacccatt cattatgct 
      tgtttgaaa aacgacatg tacttagta gaattgttg 
      cttatgaat ggtgccaga ggtgatatt ccagacgaa 
      aacggtaaa actgtcatc aattatgcc tgcaaaagt 
      gaagagtac ggtccctca attttaaag ttattgttc 
      caaagtggt ttaaaaagt gaccaaagt gttctaatt 
      gacgccgtt tcacatcac ggcattgaa attgtcaaa 
      atccttgtc gataatgcc gatatttct gacggaaat 
      cagttgtta catcatgcc tgtaagaac gaacgaaac 
      ggttacgaa atcattaaa ttcctgctt ggtaaaggt 
      gtaaaagtt gataaacct gattcagac ggcaaattg 
      ttgattcac ttaatcgca gagcgcggt tgggccgat 
      ttgattcaa aatgacaat gtgaatgtg tgtgacaac 
      tgtggtaaa atgcccatt cactatgcc tgcagtggt 
      gggcatttt accattgtc gaagtgttg attcaaaac 
      ggcgcaacg gtgaatgtg tgtgacaac tgtggtaaa 
      atgcccatt cactatgcc tgcagtggt gggcatttt 
      actattgtc gaagcgttg attcaaaac ggtgcaaag 
      gtaaatgtg tgtgatgat cggggtaaa acgcccatt 
      cattatgcc tgccaaagt gaatcaacc gaaatcgtg 
      gcgttactt attcaaaat ggcgctact gtgaatgct 
      gaagatggg gaaggcata ttaccaatt tattcctca 
      acacggagc gacattatc aagttgctt atccaaaat 
      ggggccaac gtgaatatg ccttatcca gacggaaca 
      tttttaatt cactttgct gctttgagg ggctggaca 
      tcagatatt gcaatgttt ctaattcag caaggaaca 
      gatttaaat atttatgac aaatacaac caaacggcg 
      ttacattat gcttgtcgt tacggtcgt ctggaaatc 
      acaaatttg ttgcttcaa aatggcgcc aaattaact 
      tatgatgca gatgatcaa actccaatt cactacgct 
      tgtaggtta gattctttt gatgttctc gagttgttt 
      atccaaagt ggtgcaaac ataaatatt cctggcaag 
      tttggtaaa atgcccatc catattgcc tccgaattg 
      ggaaatatc gatatattg aagaatttg atacaacac 
      ggttgtgag atcgaaatc caggacaat aaggggttg 
      tatccaatt cattacgct tgtcgcaat ccatttaac 
      ggggaccaa gttgtaaaa attttgata ttaaacgaa 
      gcaaatgtg aatgtggtt gatcatgat ggcaaattt 
      ccaatacat tatgcgtgt cagaatgag gattatggg 
      tacgatatt gttcgaata attttttcg cgacaagat 
      atcatttcg ttagaggac tataaaaca cttttcgat 
      tatgtctgc aaaaatcgt ttgaacggt tttgagatt 
      gggaaattt ttgataaag atgtcttgg ctgggtgtt 
      gttatggca cttgtccag aaaggtgtc aatagagat 
      attattgat agtaagggg agaaggcgt attgactgt 
      gtgcccaaa gattccctg cattataag gagtttctg 
      agagtgttg caaaacact gatcagata tagtttaaa 
      aaatgcaga tattaacta gagtttagt cctaaatta